Protein is an essential nutrient for humans to sustain life, but it is predicted that it will be challenging to secure protein through the traditional livestock industry in the future. Microalgae has high future value as an alternative protein food source due to resource utilization and sustainability advantages. In order to increase productivity, the culture conditions of microalgae, Chlorella vulgaris, Dunaliella salina, and Scenedesmus obliquus were examined in this study. The optimal culture conditions of C. vulgaris were mixotrophic culture, 25oC culture temperature, 7.0 initial pH, 10% initial inoculation, stirring culture, 3000 Lux light intensity, and 24L:0D light/dark cycle period with red LED. For D. salina, the optimal culture conditions were mixotrophic culture, 20oC culture temperature, 8.0 initial pH, 10% initial inoculation, stirring culture, 6000 Lux light intensity, and 12L:12D light/dark cycle period with white LED. For S. obliquus, the optimal culture conditions were mixotrophic culture, 30oC culture temperature, 8.0 initial pH, 10% initial inoculation, stirring culture, 4500 Lux light intensity, and 14L:10D light/dark cycle period with fluorescent light. These findings can be used as important information for increasing the production of microalgae as an alternative protein material resource in the future.

A new high-tech product - lab grown meat (LGM), has been gaining media attention while initiating public discourse on social media (SM) platforms. This netnographic study is based on a dataset of selected SM public posts, comments and discussions collected during 30 days in early 2023. The findings indicate that LGM is highly contradictory, while not being fully understood how it is produced or when it will become commercially available. The findings indicate that this novel food requires carefully designed marketing strategies: when naming a new product category; must allow transparency and sensibly explain all product’s attributes; and invest time and efforts to educate consumers, leading to higher adoption rates when launched on mass consumer markets, as an alternative to conventionally grown foods.

Additives in plastics are capable of migrating from the packaging materials into the foodstuffs, thereby presenting a source of contamination and a potential health risk to the consumer. The migration from packaging materials into foodstuffs is first of all regulated by examining the amounts of global and specific migrated components. Besides, there is worldwide still a need for practical methods for measuring and monitoring migration from polymers, especially for the testing of migration into fatty foodstuffs. Therefore, these studies were undertaken to investigate the safety status of domestic plastic packaging materials with respect to migration. Another objective of this study was to examine the applicability of ethanol as an alternative fatty food simulant substituting for olive oil and n-heptane. The evaporation residues for various domestic plastic samples determined as described in Korean food laws were in the level from 4.3 to 14.5 mg/l, which were much lower than the limit value of 150 mg/l. The global migration values into 95 % ethanol showed to be comparable to those into n-heptane, while the olive oil migration values were comparably higher than those into ethanol or n-heptane and moreover they were not reproducible. The kinetic migration behavior of additives in polyolefin samples into 95% ethanol showed a Fickian diffusion process. The results of these studies on global migration and kinetic testings demonstrate that the ethanol could be successfully substitute for the olive oil and n-heptane as an alternative fatty food simulant, at least in contact with polyolefins.

Biological nitrogen removal is generally accomplished by aerobic nitrification coupled with anoxic denitrification. Many commercial wastewater treatment plants (WWTPs) use external carbon source, such as methanol, to support heterotrophic denitrification process. Using organic wastes as an alternative to commercial carbon sources could thus be beneficial by saving the expense as well as reducing the environmental footprint. Here we report a full-scale (treating 2300 m3 wastewater/d) WWTP that previously utilized a butanediol-based organic waste as the sole external carbon source, which diversified the carbon sources by using a second organic waste generated from food waste recycling. Process parameters were extensively monitored for seven months at all biological unit processes, the aerobic and anoxic tanks, as well as the recirculation flow. Bacterial community structures were analyzed at anoxic tank using next-generation sequencing. The WWTP showed a stable nitrogen-removing performance over the seven months period. The estimated COD/N utilization ratio for food waste-recycling wastewater (FRW) was near 30. The bacterial populations significantly shifted during the operation. Lactobacillaceae and Prevotellaceae were the major bacterial families in the FRW, whereas the denitrification tank was populated by many families including Saprospiraceae, Nannocystaceae, Chitinophagaceae, Eubacteriaceae, and Rhodocyclaceae. Detailed discussion of the results will be presented at the conference.