본 연구는 반밀폐형 토마토 재배 온실에서 광합성율 극대화를 위한 적정 탄산가스 시비 농도를 구명하고자 광합성 모델을 이용하여 잎의 최대 카복실화율(Vcmax), 최대 전자전달속도(Jmax), 열파괴, 잎 호흡 등을 계산하고 실제 측정값과 비교하였다. 다양한 광도(PAR 200μmol·m -2 ·s -1 to 1500μmol·m -2 ·s -1 )와 온도(20°C to 35°C) 조건에서 CO2 농도에 대한 A-Ci curve는 광합성 측정 기기를 사용하여 측정하였고, 모델링 방정식으로 아레니우스 함수값 (Arrhenius function), 순광합성율(net CO2 assimilation, An), 열파괴(thermal breakdown), Rd(주간의 잎호흡)를 계산 하였다. 엽온이 30°C 이상으로 상승하였을 때 Jmax, An 및 thermal breakdown 예측치가 모두 감소하였고, 예측 Jmax의 가장 최고점은 엽온 30°C였으며 그 이상의 온도에서는 감소하였다. 생장점 아래 5번째 잎의 광합성율은 PAR 200－ 400μmol·m -2 ·s -1 수준에서는 CO2 600ppm, PAR 600－800μmol·m -2 ·s -1 수준에서는 CO2 800ppm, PAR 1000μmol·m -2 ·s -1 수 준에서는 CO2 1000ppm, PAR 1200－1500μmol·m -2 ·s -1 수준에서는 CO2 1500ppm을 공급했을 때 포화점에 도달하였다. 앞으로 광합성 모델식을 활용하여 과채류 온실 재배 시 광합성을 높일 수 있는 탄산시비 농도를 추정할 수 있을 것으로 판단된다.

The aim of the present study was to investigate the ovulation rate and its relationship to fertilization ability in Landrace, Durock and Crossbred pigs. Gilts were natural mated at a body weight of at least 120 kg under the same hormone treatment. Embryos were surgically collected 1 day after natural mating (Day 0). Embryos derived from in vivo-fertilized oocytes were cultured in medium PZM-3. The ovaries were examined and the pathological findings were recorded. The number of corpus hemorrhagicum was counted, and was assumed to equal the ovulation rate. There was no difference in the number of corpus hemorrhagicum (20.4, 28.8 and 23.2) and ovulation (13.5, 26.8 and 17.2) in the Landrace, Durock and Crossbred pigs. The two pronucleus formation was 76.0, 80.0 and 86.9%. The Day-7 embryos had blastocyst rates of 68.0, 75.0 and 73.9%. There was no difference in the number of total cells and apoptotic cells. In the future, more studies require determining relationships between ovulation and fertilization rate in different species of pigs.

신간척지인 새만금간척지에서 청보리 생산성 증대를 위한 적정 질소시비량과 파종량을 검토한 결과를 요약하면 다음과 같다. 1. 출현율은 69 ~ 77%, 월동율은 60 ~ 65% 수준이었다. 2. 초장은 질소시비량이 증가할수록 길어졌으며, 파종량간에 는 차이가 없었다. 3. 당 경수는 질소시비량 250 kg/ha까지, 파종량 300 kg/ha까 지 증가하였다. 4. 포장도복은 질소시비량 300 kg/ha와 파종량 400 kg/ha에 서 경미하게 발생하였다. 5. 생체수량과 건물수량은 질소시비량 250 kg/ha까지, 파종 량 300 kg/ha까지 증가하였다. 6. 따라서 신간척지에서 청보리재배시 생산성 증대를 위한 적정 질소시비량은 250 kg/ha, 파종량은 300 kg/ha정도가 적당 할 것으로 생각된다.

This study examined the motility of either the unattached(upper) or attached(lower) Hanwoo sperm to bovine oviduct epithelial cell(BOEC) monolayers to determine whether there are any changes in their motility during co-culture. The cleavage and blastocyst development rate were compared among different preincubation methods in-vitro, after oocytes were fertilized in-vitro with Hanwoo sperm on BOEC monolayers. The motility of frozen-thawed sperm in BOEC co-culture group was significantly higher than controls, especially at 5 hours and 6 hours (p<0.05) of incubation, in sperm treatment medium without heparin and caffeine. The motility of frozen-thawed sperm in BOEC co-culture group was significantly higher than controls, especially at 3 hours (p<0.05) and 6 hours (p<0.01), in sperm treatment medium containing heparin and caffeine. The motility of the attached( lower) sperm was significantly higher than the unattached(upper) sperm during co-culture with BOEC at all times(p<0.01 or p<0.05), except for 6 hours. After Hanwoo oocytes were fertilized in-vitro with the sperm that had been co-cultured with BOEC in sperm treatment medium containing heparin and caffeine, we determined the cleavage and blastocyst development rate, according to the preincubation methods. Both the cleavage and blastocyst development rate from 2 hour preincubation group were the highest, but significant difference was not recognized. These results show that BOEC plays an important role on sperm hyperactivation related to capacitation regardless of heparin and caffeine in sperm treatment medium. However, oviduct epithelial cell had no significant effect on the development of embryos after in-vitro fertilization in the presence of added heparin and caffeine in sperm treatment medium.

마늘에 있어서 이차생장은 구의 품위를 떨어뜨리며 심할 경우에는 작은 인편이 많이 착생하는 등 종구로 사용하기에도 어렵게 된다. 따라서 남도마늘 대주아 재배시 지온, 파종시기 및 시비량이 이차생장에 미치는 영향을 알아보고자 하였다. 시비수준별 남도마늘 대주아 재배시 생장특성을 살펴보면 초장, 엽수, 엽초경, 엽장, 엽폭은 검정시비구의 50% 증비를 하였을 경우가 가장 좋았다. 이차생장 발생율은 검정시비구의 50% 감비시 34.2%, 검정시비구에서는 44.3%, 검정시비구의 50% 증비하였을 경우는 54.1%로 시비량이 증가할수록 이차생장 발생률도 증가하였다. 수확한 구의 평균무게는 검정시비구의 50% 감비시 34.1g, 검정시비구에서는 35.1g 이었으며 검정시비구의 50% 증비시에는 33.9g로 시비량에 따른 수량변화는 없었다. 지중온도와 이차생장 발생과의 상관도를 보면 0.982~0.997로 매우 높게 나타나는 것으로 보아 남도마늘 대주아재배시 이차생장 발생은 지중온도와 상당히 밀접한 상관관계가 있었다. 파종시기가 빠를수록 초장, 엽수, 엽초경 등 지상부 생육이 좋았으며 평균구중도 9월 중순파종구가 36g, 9월 하순 파종구가 29.6g, 10월 상순처리구가 27.9g으로 파종이 빠를수록 구가 컸다. 반면 파종시기와 이차생장발생 간에는 별영향이 없는 것으로 조사되었다.

The study was carried out to investigate the effects of morphology, reproductive cycle, incubation time and activation of oocytes on in vitro maturation of cat oocytes and development of IVM/IVF embryos. The results were summarized as follows: 1. When recovered from ovaries collected at different stages of the reproductive cycle (inactive, follicular and luteal stage), the developmental rates of oocytes to GV and MI stage were 72.5% and 27.5%, 57.5% and 7.5%, 62.5% and 17.5%, respectively. 2. The developmental rates of oocytes with cumulus cells to GV and MI stage in different conditions of incubation (5% CO₂, 95% O₂ and 10% CO₂, 90% O₂) were 70.0% and 27.5%, 52.5% and 20.0%, 55.0% and 12.5%, respectively. 3. The developmental rates to GV and MI oocytes when cultured at different time of incubation (17∼20, 21∼24, 25∼28 and 29∼32 h) were 67.5% and 20.0%, 67.5% and 30.0%, 62.5% and 22.5%, 65.0% and 15.0%, respectively. 4. The fertilization and cleavage rates of freshly collected oocytes with and without cumulus cells were 72.5% and 25.0%, 37.5% and 7.5%, respectively. The rates were greater in oocytes with cumulus cells than those without cumulus cells. 5. The fertilization and cleavage rates of oocytes recovered from ovaries collected at different stages of the reproductive cycle (inactive, follicular and luteal stage) were 75.0% and 25.0%, 40.0% and 7.5%, 50.0% and 15.0%, respectively.

This experiment was canied out to study the effect of cutting frequencies (3, 4 and 5 cuts per year) and mineral nitrogen fertilization on dry matter yields, in order to estimate optimum level of fertilizing mineral nitrogen in orchardgrass(Dacry1is glome

The studies on the carried out to investigate the effects of co-culture with uterine fluids and uterine epithelial cells on the in-vitro fertilization and developmental rate of porcine follicular oocytes. The ovaries were obtained from slaughtered swine. The follicular oocytes surrounded with cumulus cells were recovered by aspirating follicular fluids from the visible follicles of diameter 3~5 mm. The follicular oocytes were cultured in TCM-199 medium containing hormones and 10% ECS for 46~48 hrs in a incubator with 5% in air at 38.5 and then matured oocytes were again cultured for 12~18 hrs with motile capacitated sperm by preincubation of heparin. The results obtained in these experiments were summarized as follows ; 1.The in-vitro maturation and fertilization rate of porcine oocytes co-cultured with uterine fluids in TCM-199 medium were 68.0% arid 55.7%, the rates were higher than of control, 56.5% arid 38.7%. 2. When the in-vitro fertilized oocytes were co-cultured with porcine uterine epithelial cells, the fertilization rate was 60.3%, the rates were higher than that of control, 35.7%. 3. When the in-vitro fertilized oocytes were co-cultured with porcine uterine epithelial cells, the development rate to be blastocyst was 12.4%, the rates were higher than that of control, 9.2%(p<0.05).

본 시험은 수단그라스계 잡종의 수량, 그루터기의 저장탄수화물 및 주수저하에 미치는 파종량과 질소시비수준의 영향을 구명하기 위하여 1983년 서울대학교 농과대학 부속실험목장의 사초시험포장에서 실시되었으며 얻어진 결과를 요약하면 다음과 같다. 1.파종량은 사초수량에 미치지 못하였으나 질소시비수준에 의한 사초수량의 차이는 고도의 유의성이 인정되었다.높은 질소시비 수준에서 최대 건물수량이 생산되었다.2.1회 예취시 파종량과 질소시비수준은 공히 저장탄수화물 수준