Feline ovulation time after LH surge have not been defined because its LH surge is occurred by several times of coital vaginal induction and cat has relatively longer time between LH surge and ovulation compared with other mammalian species. This study was performed to investigate the feline ovulation time after LH surge that was induced by hCG injection for superovulation with PMSG. For superovulation, all cats were received an initial injection of PMSG (200 IU, i.m.) followed 80 hrs later with an injection of hCG (200 IU, i.m.). And then, sampling of both ovaries was surgically performed at each 6 different times (10, 18, 22, 26, 29, and 32 hrs) after hCG injection. Cumulus-oocyte-complexes (COCs) were collected from 2 sides of oviducts and ovaries were fixed for ovarian histology. Total 38 COCs were collected only at hCG 32 hrs and no COCs were shown at earlier 5 times. However, in the ovarian histology, corpus haemorrhagicum or corpus luteum was not shown in all groups including ovary at hCG 32 hrs that COCs were collected. In conclusion, it was suggested that feline ovulation was occurred at 29~32 hrs after LH surge and taken relatively long time for CL formation after ovulation.

This is about the successful use of eCG and hCG for producing a Siberian tiger pup born from 10-year-old, primiparous, albino Siberian tiger. From February 2010 to July 2010, natural breeding had been tried three times with no conception. During this period of five months, estrus behaviors appeared to be typically normal and a lot of matings were observed. After consecutive failures, 1000 IU eCG (equine chorionic gonadotropin) were intramuscularly injected on the day showing estrus behavior, followed with an injection of 750 IU hCG (human chorionic gonadotropin) 80 hours later. The tiger stopped recurrence of estrus, and a cub, weighed 780 gram, was born alive 104 days after hCG injection. This study is the first report showing the unique, successful use of exogenous hormones as one of artificial breeding programs in the long history of captive breeding of carnivorous zoo animals in Korea.

This study was performed to investigate the effects of hCG treatment on pregnancy and delivery rates in the Hanwoo recipients. There were significantly higher pregnancy and delivery rates in the recipients treated with hCG at 7 days after artificial insemination (p<0.05), respectively. The SCNT embryos from bovine fetal fibroblast cells were transferred into the synchronized recipients. The recipients were administered saline (n=89) or hCG (1,500 IU) (n=48) at 7 days after heat, respectively. The pregnancy rate was significantly higher in the recipients treated with hCG compared to that of saline treated group (p<0.01), however, the delivery rate was not different in both treated groups. The concentration of plasma progesterone (P4) was not different in both groups before hCG treatment, but the P4 level was increased significantly in hCG treated group after hCG injection (p<0.05). Although the pregnancy rate was very high in early stage of pregnancy, it was decreased dramatically after 50 days of pregnancy and maintained basal level. Taken together, the treatment of hCG in the SCNT recipients after day 7 of heat was effective method to increase the P4 concentration and to increase the pregnancy rate. But it did not affect directly to delivery.

무발정기에 속한 Shih-tzu 견 9두를 대상으로 하여 PMSG(50IU/kg)를 10일 동안 매일 근육주사 후 마지막 10일째에 hCG(1,000IU/head)를 정맥 주사하여 인공적으로 발정을 유기 하였다. 실험견 9두 중 9두(100%)에서 발정 출혈, 회음부 반사, 외음부 종대 및 수컷 허용 등의 임상적인 발정 증상이 관찰되었으며 그중 5두(55.6%)가 임신하였고 4두는 임신되지 않았다(44.4%). 임신견 5두 중 3두(33.3%)가 자연분

본 연구는 한우미경산우에 인공수정 7일째와 복제수정란 이식시 hCG를 투여에 의해 수태율 향상을 위하여 실시하였다 인공수정 후 임신 60일째에 임신감정을 실시한 결과 대조구, CIDR 그리고 hCG를 투여한 결과 53%, 46%, 71%였으며, 처리간에 유의적인 차이를 보였다(P<0.05). 복제수정 란의 이식시 hCG를 투여한 결과 대조구, hCG투여구는 각각 5.8%, 10.4%를 나타냈으며, 유의차는 인정되지 않았다. 인공수정 7일째에 hCG를 투

This study was undertaken to test the hypothesis the hypothesis that treatment with hCG (5,000 IU) at the time of embryo transfer would enhance pregnancy rates in recipients, and the concentration of plasma urea nitrogen(PUN) in recipients was related to the effect of hCG on the reproductive performance. Blood samples were taken according to experimental condition for the assessment of the endogenous plasma progesterone concentration and plasma urea nitrogen. Concentrations of progesterone in plasma were higher in cows treated with hCG on day 7(estrus=day 0) than in those untreated during 7∼43 days after insemination. The pregnancy rates were 65.5 and 54.6% for the hCG treated and untreated groups, respectively. In recipient group categorized with PUN concentration of <12 mg/이, the pregnancy rates were 68.8 and 46.7% for the hCG treated and untreated groups, respectively. The results suggest that hCG treatment at 7 days after insemination could be used to increase the pregnancy rate of embryo transfer, and transfer, and only the recipients with PUN concentration of <12 mg/dl were influenced by treatment with hCG.

We performed VRI CCD surface photometry of three spiral galaxies of HCG10 in order to understand the effect of interactions in the compact group. The morphology of the largest member NGC 536 seems to be normal but its bulge is thought to be of an oblate spheroid. The central surface brightness of the disk of NGC 536 is much fainter than that of disks of nearby spiral galaxies. The morphologies of NGC 531 and NGG 542 appear to be affected by interactions which lead to a warped disk in NGC 531 and a slightly bent disk in NGC 542. NGC 531 have a boxy bulge and a Freeman Type II disk both of which strongly suggest the presence of a bar in the galaxy. There is a break in the slope of the luminosity profile of NGC 542 which is dominated by the disk component.

임신랫드에 융모성성선자극호르몬을 투여한 후 모체의 황체와 태아에 미치는 영향을 조사하기 위하여 임신랫드에 융모성성선자극호르몬제제인 Profasai 1000(Serona, Switzland) 50 또는 100IU를 임신 4, 8, 12 또는 16일에 각각 투여한 후 임신20일에 부검하여 태아와 모체에 대한 조사를 한 결과를 요약하면 다음과 같다. 1. 황체는 모든 대조군과 HCG투여군에서 그 수가 거의 비슷한 수준이었고 임신8일의 경우에 그 수가 비교적 감소되었다. 2. 태아의 흡수는 임신 4, 8및 12일의 HCG투여군과 임신 12일의 대조군에서 나타났지만 임신4일의 경우가 발생빈도가 현저하게 높았고 임신 12일의 경우는 매우 낮았다. 3. 태아의 체중은 임신4일에 50IU의 HCG투여군에서만 감소되었다. 4. 태아의 골격검사에서 기형발생과 골화의 이상은 인정되지 않았지만 변이는 인정되었다. 변이는 14번늑골만 인정되었으며, 임신 4, 8 그리고 12일의 HCG투여군과 임신8일과 16일의 대조군에서 다양하게(5.26~8.33%) 나타났다. 5. 임신랫드에 HCG를 투여하면 태아의 흡수가 나타날 수 있지만 골격형성 과정에서 반드시 변이가 발생된다고 생각하지 않는다.