Marigold powder is recognized to be a good source of lutein that plays significant roles in promoting the health of eyes and reducing age-related cataract risk. Because lutein cannot be synthesized in a human body, it needs to be supplied from the diet. However, there are no lutein-incorporated food products in a market other than capsules or powders. In this study, the processing performances of marigold powder in a fresh noodle system was investigated in terms of pasting, viscoelastic, and textural properties. In addition, the levels of lutein in the marigold powder-incorporated fresh noodles were quantitatively analyzed before and after cooking by HPLC. The pasting and dynamic viscoelastic parameters of wheat flour were reduced with increasing levels of marigold powder. The SEM images demonstrated that the wheat flour paste samples with higher levels of marigold powder show a highly cellular structure. However, the use of marigold powder at a level of 2% did not significantly affect the tensile properties of fresh noodles. When marigold powder was incorporated into the formulation of fresh noodles, their lutein contents were ranges from 61.22 to 204.91mg/100 g noodles. After cooking, the lutein contents were reduced by almost 50% while they seemed to satisfy the recommended dietary intake of lutein.

Relaxin과 insulin이 돼지 난포 과립막세포의 스테로이드 호르몬 분비에 미치는 영향을 연구하기위하여 체외에서 황체화된 과립막세포에서 prosesterone과 의 생산을 조사하였다. 돼지난포 과립막세포를 혈청 존재하에 배양접시에 부착 후 48시간 동안 체외배양하고 무혈청 배지에서 24시간 배양하였다. Relaxin과 insulin의 용량의존성을 확인하기 위하여 다양한 농도 (10, 100, 1,000 ng/ml)를 각각 무혈청 배지에 첨가하였다.

Background : Plants are the rich source of antioxidants, which plays a very important role in maintaining human health. Their antioxidant property protects cells of different organs of human beings against free radicals and free radical mediated diseases. Even though, there is lack of knowledge on the antioxidant effect of lutein present in plants. In the present study, lutein was isolated from the GreenTea leaves (Camellia sinensis) which is used as a dietary source. Methods and Results : The procedure adopted for the isolation and purification of lutein using acetone extraction and preparative high performance liquid chromatography (HPLC) is simple and less time consuming. Free radicals scavenging activity of isolated lutein from acetone extract of GreenTea was assessed by DPPH radical scavenging assay and reducing power. The isolated lutein scavenged 79% of DPPH radicals at 20 ㎍/㎖ and two fold lower concentration compared to the standard antioxidants (α-tocopherol). No significant differences were found between the reducing power of the lutein and BHT when their concentrations were high. However, significant differences were observed at relatively low concentrations, the reducing power of lutein was isolated from the GreenTea leaves was stronger than those of their acetone extract and standard antioxidants (BHA). Both electron spin resonance (ESR) and in vitro assay confirmed that lutein was isolated from the GreenTea leaves, exhibited a greater capacity for scavenging superoxide (O2 •－) and hydroxyl (OH •) radicals than standard antioxidants β-carotene and α-tocopherol respectively. Conclusion : The results proven that lutein isolated from GreenTea leaves has an efficient antioxidant ability, it could serve as an antioxidant to scavenge reactive oxygen species.

Background : Lutein, a xanthophyll, consists of chains with 8 conjugated double bounds containing closed rings on each end of the chain. This carotenoid is found in fruits and vegetables, especially dark green leafy vegetables such as green tea. In this study, we investigated the anticancer effects of purified lutein from green tea on human cancer cell lines containing prostate carcinoma cancer cells (LNCaP). Methods and Results : Prostate carcinoma cancer cells (LNCaP) were cultured and evaluated the inhibitory effect of lutein isolated from green tea compared other carotenoids (β-carotene and lycopene) on cell proliferation. Cyclin D1 and PCNA were evaluated as cell differentiation. In results, PCNA/cyclin regulates the initiation of cell proliferation by mediating DNA polymerase. Under cultural conditions, lycopene remarkably suppressed the PCNA expression prostate cancer cell line LNCaP in higher doses (20 μM - 100 μM) statistically. However, β-carotene and lutein presented the less inhibitory effects on PCNA expression. Determination of PCNA expression in control and treated cells demonstrates that lycopene did affect proliferation in LNCaP cancer cells in dose-dependent manner. However, β-carotene and lutein suppressed the cyclin D1 expression in dose-dependent manner but no in lycopene group. These results indicate that differ carotenoids presented the various suppressive ability of PCNA and cyclin D1 expression in cell proliferation. Conclusion : In conclusion, lutein suppressed the carcinogenesis of induced prostate cancer cell line by acting as a suppressor for inhibiting the expression of cyclin D.

GnRH는 국부적으로 난소에서 합성되며, 난소내 과립 및 황체세포에 직접적으로 작용하여 난소의 기능을 조절하는 것으로 알려져 있으며, 특히, GnRH는 난소내 과립-황체화 세포의 세포자연사를 유도하는 것으로 보고하고 있다. 그러나 GnRH에 의한 세포자연사가 FSH에 의해 회복될 수 있는지는 명확히 밝혀져 있지 않다. 따라서 본 실험에서 난자 채취시 획득한 사람 과립-황체화 세포를 배양한 후 5, 50, 100 ng/ GnRH와 1 IU/ FSH를 처리

Cortisol은 난소내 다량으로 존재하며, 난소 세포에 그 수용체가 있는 것으로 보고되고 있다. 또한 사람의 과립 및 황체화 세포에서 cortisol은 스테로이드 생성과 세포 대사에 영향을 미치는 것으로 알려지고 있으나, 배란 후 난포액에 높은 농도로 존재하는 cortisol이 과립-황체화 세포에 어떤 영향을 미치는 지는 정확히 밝혀져 있지 않다. 따라서 본 실험에서 과배란 유도후 획득한 사람 과립-황체화 세포를 배양하면서 5, 50, cortisol

Black soybeans, which have been widely utilized as food and as material for Oriental medicine, contain anthocyanins in the seed coat. Soybean seeds with black seed coat and green cotyledon are called “Seoritae” in Korea. Korean customer prefer “Seoritae” to normal black soybean with yellow cotyledon. The pigments contained in green cotyledon of black soybeans were chlorophylls and lutein. These constituents function as antioxidant and protect humans against diverse damages. The purpose of this study was to investigate the most suitable extraction condition and method of green cotyledon pigments in black soybeans. The green cotyledon pigments were analyzed on RP-HPLC with C18 column using gradient system. The gradient system was used two mobile phases. A gradient elution was performed with mobile phase A, consisting of 100% MeOH, and mobile phase B, consisting of 100% EtOAc. Among the 4 kinds of extraction method using methanol and/or acetone solution, ultrasonic extraction at 30 min. using acetone solution was most suitable extraction condition for simultaneous analysis of chlorophylls and lutein in soybean with green cotyledon.