Feline ovulation time after LH surge have not been defined because its LH surge is occurred by several times of coital vaginal induction and cat has relatively longer time between LH surge and ovulation compared with other mammalian species. This study was performed to investigate the feline ovulation time after LH surge that was induced by hCG injection for superovulation with PMSG. For superovulation, all cats were received an initial injection of PMSG (200 IU, i.m.) followed 80 hrs later with an injection of hCG (200 IU, i.m.). And then, sampling of both ovaries was surgically performed at each 6 different times (10, 18, 22, 26, 29, and 32 hrs) after hCG injection. Cumulus-oocyte-complexes (COCs) were collected from 2 sides of oviducts and ovaries were fixed for ovarian histology. Total 38 COCs were collected only at hCG 32 hrs and no COCs were shown at earlier 5 times. However, in the ovarian histology, corpus haemorrhagicum or corpus luteum was not shown in all groups including ovary at hCG 32 hrs that COCs were collected. In conclusion, it was suggested that feline ovulation was occurred at 29~32 hrs after LH surge and taken relatively long time for CL formation after ovulation.
The aim of this study was to estimate of ovulation time and parturition day at the same time as breeding in small dog by vaginal cytology and to confirm the accuracy by comparing the expected parturition day and the real one. Characteristic features of vaginal cytology during the estrous cycle were the high proportion of large intermediate cell, superficial cell, anuclear cell and erythrocyte in proestrus, superficial cell and anuclear cell in estrus, parabasal cell, small intermediate cell, large intermediate cell and leukocyte in diestrus, parabasal cell and small intermediate in anestrus, respectively. When day 0 was the parturition day, the period of pregnancy is 67.45(64~75) days when the cornification index (CI) is over 90%. Also, on the basis of ovulation day, 63.65(59~66) days was confirmed, and 57.0(52~60) days was confirmed based on the first day of diestrus. There are the gap of 4 days between the day being over 90% in CI and ovulation day. On the basis of this, when expecting parturition day based on the day being over 90% in CI by vaginal cytology, 18.1% was produced in the same of the expected parturition day and the real one, 30.3% and 33.3% were produced in the gap of one day and two days, respectively so, the accuracy within two days was 81.7%. In addition, based on the first day of diestrus, it also was identified to 81.7% as the difference between the expected parturition day and the real one within 2 days. It demonstrated there are any difference between any expected parturition day by vaginal cytology. Thus on the basis of the day of being over 90% CI, it is fully thought to using clinically due to the possibility of prediction the parturition day at the same time as the determination of the proper time of the optimal mating time.
본 연구는 젖소 경산우와 미경산우의 다양한 발정 행동 특성과 배란 시간을 조사하기 위해 수행되었다. 공시된 89두 중 73두에서 배란이 일어났으며, 이 중 61두에서 발정 발현이 나타났다. 다양한 발정 행동은 투여 후 2일째부터 72시간 동안 육안 관찰하였으며, 배란 시간(4시간 간격으로 초음파 시험)과의 관계를 각각 조사하였다. 경산우의 발정 발현율은 외음부 냄새 맡기, 턱 비빔, 승가 및 승가 허용에서 각각 81%, 78%, 78% 및 56%로 나타
본 연구는 젖소의 발정 행동 징후에 따라 배란 시간을 예측하고 결정하기 위하여 발정의 다양한 행동 징후(승가, 승가허용, 외음부 냄새 맡기 및 턱비빔)와 배란 시간과의 관계에 대해 조사하였다 공시된 51두 중 37두에서 배란이 일어났으며, 이 중 28두에서 발정 발현이 나타났다. 발정 행동 징후는 주사 후 2일째부터 72시간 동안 육안 관찰하였으며 배란 시간(4시간 간격으로 초음파 검사)과의 관계를 각각 조사하였다. 발정 행동별 발정 발현율은 외음부 냄
Shit-tzu견에서 발정 주기, 교배 적기 및 배란 시기를 정확하게 추정하기 위한 질 세포 검사를 확립하기 위하여 Shih-tzu 견 12두를 대상으로 발정 주기동안 질 세포 검사 및 estradiol-와 progesterone 농도를 측정하여 다음과 같은 결과를 얻었다. 발정주기별 질 세포상의 특징적인 변화로서 발정 전기에는 무핵 세포와 적혈구, 발정기에는 표층 세포와 무핵 세포 및 적혈구가 주종을 이루는 세포였으며 발정 휴지기에는 소형 중간 세포와