한국의 경제 발전에 따라 양돈 사업의 규모가 커지고 대단지화 되면서, 생산성 향상과 높은 품질을 충족시키기 위해 축사시설의 체계적인 관리가 요구되고 있다. 그러나 효율성을 위해 밀집된 구조에서는 종종 여름철에 공기의 질이 나빠지고 온도가 높아지는 등의 문제를 일으킬 수 있다. 돼지는 저산소 및 고온에 매우 취약하기 때문에 생산성을 높이기 위한 환기 유지는 매우 중요하다. 이에 본 연구에서는 전산유체역학(CFD, computational fluid dynamics)을 이용하여 환기 시스템의 문제점 및 개선 사항을 분석하는 방법을 제안하였다. 유한체적법(FVM, finite volume method) 기반의 fluent 프로그램이 사용되었고 난류 모델로 RNG standard k-ε 모델을 사용하였다. 환기 시스템의 효율적인 설계를 위해 실제 단층의 단순 구조 양돈사와 다층의 대형 양돈사를 대상으로 다종물질이송(multispecies transport) 해석기법을 이용하여 환기 효율 및 성능을 분석하였으며, 개선된 시스템의 유동순환이 원활하게 이루어짐을 확인하였다.

Eighty pigs (40 pigs per farm aged 40 days old) that had been raised on two commercial pig farms A and B were used to evaluate oxidative stress status. The results from each farm were compared to investigate a relationship between pig performance and oxidative stress status. Pig performance on farm A was relatively better than that on farm B for the period of 3 years. The level of plasma total antioxidant status (TAS) of the pigs in group 1 (farm A) was significantly higher (p=0.045<0.05 ) than that of the pigs in group 2 (farm B). The level of plasma total oxidant status (TOS) and oxidative stress index (OSI) value of the pigs in group 2 were significantly higher (p= 0.045<0.05 and p=0.001<0.05) than those of the pigs in group 1 These results revealed that pig performance was associated inversely with oxidative stress status.

Alzheimer's disease (AD) has caused by expression of amyloid precursor protein (APP), Tau and presenilin (PS) as known as plaques and tangle accumulation. AD transgenic porcine model is necessary for preclinical testing of therapeutic agent because of similar metabolic system between porcine and human. The objective of study was to generate AD transgenic pig by somatic cell nuclear transfer (SCNT) with multi-cistronic vector system. AD multi-cistronic vector was 6 well-known mutation on 3 AD related genes, hAPP (K670N/M671L, I716V, V717I), hTau (P301L) and hPS1 (M146V, L286P). Establishment of AD transgenic cell lines was used from Jeju black pig ear fibroblast cells (JB-PEFAD) with the AD multi-cistronic vector. The JB-PEFAD cell was confirmed on mRNA expression, protein synthesis of hAPP, hTau and hPS1 and identification of integration and karyotype. Although fusion rate was no difference in SCNT with JB-PEF AD (SCNTAD) embryos, cleavage and blastocyst formation rates were slightly lower than in SCNT with non-transgenic JB-PEF (SCNTnon-TG). Individual SCNTAD blastocysts were detected hAPP, hTau and hPS1 genomic integration which showed 93.2% (n=30) efficiency in genomic DNA (gDNA) level. It will give us a possibility to develop porcine animal model for AD study in the future.

The relationship of several candidate genes polymorphisms with breeding values of economic traits were investigated in Korean Native Pigs. Record (2001-2006) of 546 Korean native pigs were obtained from National Institute of Animal Science, Korea having data on average daily gain (ADG), age at 70 kg (D70 kg) and backfat thickness (BF). The data's obtained were analyzed by the DF-REML (Derivative-Free Restricted Maximum Likelihood) program of Boldman using a single-trait animal model to analyse the genetic parameters. The analysis of restriction fragment length polymorphism (RFLP) was conducted on 68 Korean native pigs (KNP) using single nucleotide polymorphism (SNP). Different genotype frequencies of 5 candidate genes such as MC4R, PRKAG3, FABP3, ESR and PRLR3 were observed in KNP. Significant relationship of AA and AB genotype between MC4R polymorphic site and breeding value for average daily gain (ADG, p<0.05) was observed. PRKAG3 polymorphic sites were also found to be significantly related to breeding values for ADG, AA, AB genotype (p<0.05) and also, for Backfat thickness (BF), days to 70 kg and BB genotype (p<0.05). In conclusion, selection method would be more effective if it encompasses significant genotype for performance traits and that would further aid in the selection of seed stock in KNP.

This study was conducted to determine the effects of dietary probiotics BiopowderR (BP) on pig performance, carcass characteristics and ammonia concentration in feces. A feeding trial was conducted with a total of 36 growing pigs of an average 29kg body weight. Each treatment had 4 pigs with 3 replications. Pig during grower (26days) and finisher periods (62days) were fed 0.15 and 0.30% concentrations of BP respectively. Body weight gain slightly increased for pigs fed BP during overall periods compared with control, not significantly different. Especially, in growing periods, the body weight gain of BP treatment increased by 10% compared with control (700g vs 775g), in finishing periods, that of BP 0.3% treatment increased by 10% compared with control (890g vs 980g), not significantly different among treatments. Feed intake slightly increased for pigs fed BP in overall periods. During grower periods, the feed intake of BP 0.3% treatment increased by 11.0% more than control, but also not significantly different among treatments. Feed efficiency was slightly improved for pigs fed BP overall periods when compare to the control, but not significantly different among treatments. In feces, average ammonia concentration (ppm) and bacterial count (log cfu/g) were not affected by BP addition. There was no significantly different in carcass weight, fat thickness and carcass grade. In conclusion, supplemental BP tended to slightly improve the performance of pig and did not influenced the concentration of ammonia in feces and carcass characteristics.