The objective of this study is to investigate the changes in concentrations of blood urea nitrogen (BUN) and sex steroid hormones, such as estrogen, progesterone, and testosterone in Korean cattle (Hanwoo) with reproductive disorders and to examine the relationship between BUN and body condition score (BCS) in Hanwoo. The concentration of BUN was 16.2 mg/dl, 17.8 mg/dl, 15.1 mg/dl, 17.9 mg/dl, and 28.3mg/dl in pregnancy, repeat breeding, follicular cyst, luteal cyst, and ovarian atrophy, respectively. In Hanwoo with BCS , and , the concentration of BUN was 15.8 mg/dl, 17.0 mg/dl, and 17.6 mg/dl, respectively. Fluoroimmunoassay showed that serum estrogen and progesterone levels were decreased in reproductive disorders Hanwoo, such as ovarian atrophy, endometritis, and weak estrus. The testosterone level was significantly decreased in Hanwoo with reproductive disorders compared to that in pregnant Hanwoo ( vs 0.13 ng/ml, p<0.05). The progesterone and estrogen concentrations in follicular fluid obtained from ovary with follicular cyst were significantly higher (p<0.05) than those in normal follicle fluid. These results show that there is no relationship between BUN and BCS in Hanwoo, and the concentration of sex steroid hormone in serum and follicular fluid are changed in reproductive disorders Hanwoo.
Our previous research has identified granulin (grn) and p130 genes as sex steroidinducible genes in the rat hypothalamus, which might be involved in sexual differentiation of the brain. Phthalate esters that are used as plasticizers and also found at low levels in foods such as dairy products are often mentioned as suspected endocrine disrupters. The purpose of the present study is to elucidate whether perinatal exposure to dinbutyl phthalate (DBP), diisononyl phthalate (DINP) and di2ethylhexyl adipate (DEHA) affects hypothalamic sex steroidinducible genes. The present study assessed the effects of perinatal exposure to DBP, DINP and DEHA on sex steroid hormones levels and hypothalamic grn and p130 mRNA expressions at postnatal day (PND) 3 and 7. Pregnant rats were fed a soyfree diet containing 20, 200, 2,000 and 10,000 ppm of DBP, 40, 400, 4,000 and 20,000 ppm of DINP, or 480, 2,400 and 12,000 ppm of DEHA from gestational day (GD) 15 to GD 3 or 7. At PND 3 and 7, perinatal exposure to these chemicals did not substantially affect serum concentrations of testosterone and estradiol. At PND 3, the expression of grn mRNA levels in males was decreased by DEHA, and that of p130 was decreased by DBP, DINP and DEHA, though the effects were not dosedependent. At PND 7, the expression of grn gene in female pups was increased by higher doses of DBP and all the doses, except for 4,000 ppm, of DINP, while that in male pups decreased by 480 and 12,000 ppm of DEHA. Hypothalamic expression of p130 mRNA in males was increased by lower doses of DBP and all the doses of DINP, whereas that of females was decreased by 480 and 2,400 ppm of DEHA. These results suggest that these chemicals may affect the expression of grn and p130 genes by directly acting on the hypothalamus, thus leading to inappropriate expression of these genes.
These studies were undertaken to examine the interaction of tamoxifen with sex steroid hormones in rat uterine activity. The uterine wet weights of the immature Tat uterus were examined after the administration of estradiol-l7(1g), tamoxifen(50g), progesterone(lmg). The uterotropic activity in immature ovariectomized rats was observed under various treatment conditions following pretreatment with above drugs. The results obtained were as follows:1) Tamoxifen produced significant increase (p <0.01) in uterine wet weight compared with control group, although the increase was not as great as that seen with estradiol-17. Administration of estradiol-17 together with tamoxifen inhibited significantly the increase of uterine wet weight by estradiol-17 (p < 0.01). Coadministration of progresterone with tamoxifen partly blocked the increase of tamoxifen-induced uterine wet weights by progesterone. 2) Estradiol-17after the estradiol-17 pretreatment discontinued the declining uterine wet weights due to the absence of estrogen support, but uteri continued to increase in weight if daily estradiol-17 was maintained. Administration of tamoxifen on the fourth day of estradiol-17 treatment reduced uterine wet weights within 24 hours, and the weights continued to decline with additional tamoxifen. 3) The modest growth of the uterus induced by three daily injections of 5Opg tamoxifen remained stable for five days, with or without additional tamoxifen treatment. Coadministration of tamoxifen with estradiol17 increased slightly the increase of uterine wet weight by tamoxifen. Coadministration of tamoxifen with progesterone inhibited the increase of uterine wet weight by tamoxifen. 4) The modest growth of the uterus induced by three daily injections of lmg progesterone reduced uterine wet weight to the control level for five days. Commencement of tamoxifen or estadiol-17 injections on the fourth day of progesterone treatment rapidly elevated uterine wet weight.
The gonadosomatic index (GSI), gonadal development and changes in hormones in plasma level of the indoor cultured grunt (Hapalogenys nitens) were investigated by histological study from August 2011 to October 2012. The GSI showed similar trends with gonad developmental stages during the culture periods. Changes in plasma level of estradiol-17β of female H. nitens reached the highest value before the spawning period, and seasonal changes in plasma level of estradiol-17β were similar in trends of oocyte developments and GSI changes. Testosterone levels of male H. nitens reached the highest value before and after the spent stage. Ovarian developmental stages of H. nitens could be classified into early growing stage, late growing stage, mature stage, ripe and spawning stage, recovery and resting stage. The testicular developmental stages could be divided into growing stage, mature stage, ripe and spent stage, and recovery and resting stage.
We investigated the change mRNA expression of GtHs subunits (FSHβ, LHβ) in the pituitary, androgen receptor (AR), estrogen receptor (ERα) in gonad and histological observation of gonads in longthooth grouper Epinephelus bruneus by treatment Femara, an aromatase inhibitor (AI). Longtooth grouper (body weight 408±43.1 g; one year) cultured in Future Aquaculture Research Center, NFRDI were used in the experiments. The experiment was conducted for 12 weeks from 21 August 2013. Fish received intramuscular injection of AI at 5 mg/g BW dose in three times every 3 weeks. Fish were sampled pituitary and gonads at 3, 6, 12 weeks post-injection (n=50). The mRNA levels of FSH-β, LH-β in pituitary and AR, ERα mRNA in gonad were evaluated using qRT-PCR and qPCR. The histological change of gonads observed on light microscope. The gonads of control group contained most perinucleolus oocyte. At 3 to 6 weeks post-injection, the gonads of AI-treated group contained a few degenerated oocytes, spermatogonia and spermatocytes. At 12 weeks post-injection, gonads contained spermatids undergoing spermatogenesis. From 6 to 12 weeks post-injection, the expression level of GtHs subunits mRNA in pituitary was significantly higher than control group. The expression level of AR mRNA in gonad was higher than control group from 3 to 12 weeks post-injection. The expression level of ERα mRNA in gonad was lower than control group from 6 to 12 weeks post-injection. These results suggest that immature longtooth grouper with AI treatment induced masculinization via change of GtH subunits in pituitary, AR and ERα mRNA in gonad.
We investigated the changes in plasma sex steroid hormones, testosterone (T), estradiol- (), 17,-dihydroxy-4-pregnen-3-one (), 11-ketotestosterone (11KT) and cortisol levels from ribbed gunnel, Dictyosoma burgeri in associated with annual reproductive cycle. The gonadosomatic index (GSI) of females increased from November, peaked in February and decreased rapidly from March. The GSI of males also increased from November, peaked in January and then decreased gradually. In females, levels increased and remained high from December to February. The levels of T showed a similar tendency and correlated (=0.898, p<0.01) with levels. The levels of increased rapidly in February () and peaked in July (). Cortisol level was peaked in March and correlated with levels (=0.696, p<0.01). In males, the levels of T was peaked in January and then decreased rapidly. The levels of 11KT were remained high from October to January. On the other hand, the levels of fluctuated during reproductive cycle. These results suggest that plasma sex steroids in ribbed gunnels have annual periodicity, and that cortisol may involve in maturation of females.
We investigated the changes in plasma sex steroid hormones, testosterone (T), estradiol-17β (E2), 17,20 β-dihydroxy-4-pregnen-3-one (17α20βOHP), 11-ketotestosterone (11KT) and cortisol levels from ribbed gunnel, Dictyosoma burgeri in associated with annual reproductive cycle. The fish were collected at the coastal waters of Dadaepo, Busan, Korea from August 2002 to March 2003. Body weight, liver weight and gonad weight were measured for calculation of hepatosomatic index (HSI) and gonadosomatic index (GSI). Plasma steroid and cortisol levels were measured by radioimmunoassay and data were analyzed using the Kruskal-Wallis test and the Spearman rank correlation. The GSI of females increased from November, peaked in February (13.21±2.27) and decreased rapidly from March (1.05±0.23). The GSI of males also increased from November, peaked in December (0.36±0.12) and remained high to February. In females, E2 levels increased and remained high from December to February (19.81±7.46~22.85±6.18 ng/ ㎖). The levels of T showed a similar tendency and correlated (rs=0.813, p=0.0001) significantly with E2 levels. The levels of 17α20βOHP increased rapidly in February (4.78±1.01 ng/㎖) and peaked in July (5.08±0.65 ng/㎖). Cortisol level was peaked in March (2081.07±1140.02 ng/㎖) and correlated significantly with 17α20βOHP levels (rs=0.790, p=0.0001). In males, the levels of T was peaked in January (2.43±0.17 ng/㎖) and then decreased rapidly. The levels of 11KT were remained high from October to January (3.27±1.55~4.28±1.53 ng/㎖) and correlated significantly with T levels (rs=0.513, p=0.002). On the other hand, the levels of 17α20βOHP fluctuated during reproductive cycle and the variation pattern of cortisol levels were similar to females. These results suggest that plasma sex steroids in ribbed gunnels have annual periodicity, and that cortisol may involve in maturation of females.
내분비 교란물질들이 연안 어류의 정상적인 성적 발달을 위협할 수 있다는 사실은 잘 알려져 있지만, 해산 어종에 대한 이러한 위협을 파악하는 데 이용할 수 있는 적절한 시험 어종은 아직 개발되지 못했다. 그 이유 중의 하나는 성분화 초기에 어류의 생식소가 외형으로나 조직학적으로 암수 구분이 불분명한 경우가 많기 때문이다. 본 연구에서는 조피 볼락 Sebastes schlegeli의 자연 성비, 생식소 구조, 그리고 외인성 성스테로이드 호르몬에 의한 성분화
웅성 선숙형 해산 어류인 감성돔에게 를 경구 투여하여 성스테로이드인 testosterone(T), 및 cortisol 수준의 변화와 생식소 발달을 조사하였다. 외인성 는 사육 60일째 감성돔의 혈장 T의 수준을 유의하게 상승시켰으나, 혈장 및 난모세포의 크기에는 영향을 미치지 못하였다. 또한 처리는 생식소 내 정소 발달과 정자 형성과정을 촉진시켰으며, 정자 방출 기간을 연장하는 것으로 나타났다. 를 처리한 실험구에서 60일째에 혈장 cortisol 수준