To investigate the optimum condition of 3-monochloro-l,2-propanediol(MCPD) analysis, gas chromatography with electron capture detector was-used. Determination of MCPD derivatized with phenylboric acid was more effective than that of underivatized MCPD. In derivatization of MCPD with phenyl boric acid, there were no significantly different between boiling for 2min at 90℃ and vortexing for Smin at room temperature. Extrelut column was suitable for extraction of MCPD diluted in 20% NaCl solution and recovery rates were higher than direct extraction of MCPD with ethyl acetate. But, the method of direct extraction of MCPD with ethyl acetate was useful for rapid and qualitative analysis. The sample extracted in soysauce(ganjang) was derivatized with phenylboric acid and analyzed by gas chromatography-mass selective detector. That was confirmed as MCPD-phenylboronate.

The SSH100-10 bacterial strain, which exhibits strong antifungal (anti-mold and anti-yeast) activity, was isolated from traditional korean soysauce aged 100 years. The strain was identified as Bacillus velezensis based on Gram-staining, the biochemical properties and 16S rRNA gene sequence determination. B. velezensis SSH100-10 showed strong proteinase activity and NaCl tolerance, but did not produce enterotoxin. Two-antifungal compounds from B. velezensis SSH100-10 were purified using SPE, preparative HPLC, and reverse phase-HPLC. The purified antifungal compounds were identified as C14 and C15 iturin through MALDI-TOF-MS and amino acid composition analysis. The stability characteristics of the antifungal compounds after temperature, pH, and enzyme treatments suggested that B. velezensis SSH100-10 produced more than two antifungal compounds; pH-stable C14 iturin A and C15 iturin A, and unidentified pH-unstable compounds. The results suggested that B. velezensis SSH100-10 can be used in soybean fermentation as a starter. Moreover it has potential as a biopreservative in the food and feed industry and as a biocontrol agent in the field of agriculture.

The Soysauce wastewater removal characteristics of submerged biofilters filled with two filter media respectively were experimentally examined with constant temperature, pH value and variable BOD loading and recirculation ratio. The decreasing order of BOD removal is Netring(random plastic media), cubic wire meshes(plastic module). This is mainly due to the media characteristics such as void ratio, specific surface area and media shapes. The BOD removal ratio decreases with increasing BOD_5 volumetric loading rate, and the loading rate for the BOD removal over 85% is lower than 1.5㎏BOD_5/㎡d for the plastic media of Netring and cubic wire meshes. The BOD_5 removal rate increases with the recirculation ratio, but the rate of increase become smaller as the recirculation ratio increases over 20. When BOD_5 volumetric loading is 1.5㎏BOD_5/㎡d, the required recirculation ratio to obtain 85% BOD_5 removal is about 20 for Netring and it was about 30 for cubic wire meshes.