Cucumber mosaic virus (CMV) poses a considerable threat to a diverse array of crops in global agriculture. CMV impacts commercially important cut lilies by diminishing both yield and flower quality. We used RNA sequencing (RNA-seq) to investigate the changes in gene expression in the leaves and bulbs of four distinct cultivars of cut lily, ‘Cancun,’ ‘Brunello,’ ‘Connecticut King,’ and ‘Casa Blanca’ following CMV infection. Notably, CMV affected photosynthetic processes by significantly downregulating genes associated with photosynthesis. In addition, CMV infection was detrimental to chloroplast function and energy production. We observed differential expression of genes associated with both dominant and recessive resistance pathways that are crucial for preventing virus entry, replication, and systemic spread within the plant. Based on functional annotation and differential gene expression analysis, we identified the regulatory genes involved in triggering immune responses, modulating signal transduction, and specific host factors during CMV infection. To validate the RNA-seq findings, we selected four genes involved in resistance, virus multiplication, and virus spread and analyzed them using real-time quantitative reverse transcription PCR (qRT-PCR) with specific primers. The qRT-PCR results aligned closely with those from RNA-seq, showing consistent fold-change responses for the genes that were differentially expressed, indicating that the RNA-seq results were reliable. These results deepen our understanding of the complex genetics behind plant-virus interactions while also providing information for breeding programs that aim to develop CMV-resistant lily cultivars.

한반도 애호랑밑빠진버섯벌레속(Baeocera Erichson, 1845)에 대하여 논의하고 검색표를 제공하였다. 참애호랑밑빠진버섯벌레(Baeocera choi Hoshina and Park, 2011)는 배애호랑밑빠진버섯벌레[Baeocera ventralis (Löbl, 1973)]의 동물이명 임이 밝혀졌다. 이들의 수컷생식기 형질 을 도해하고 근연종과 함께 동정에 유용한 형태형질을 제시한다.

High-entropy alloys (HEAs) represent a revolutionary class of materials characterized by their multi-principal element compositions and exceptional mechanical properties. Powder metallurgy, a versatile and cost-effective manufacturing process, offers significant advantages for the development of HEAs, including precise control over their composition, microstructure, and mechanical properties. This review explores innovative approaches integrating powder metallurgy techniques in the synthesis and optimization of HEAs. Key advances in powder production, sintering methods, and additive manufacturing are examined, highlighting their roles in improving the performance, advancement, and applicability of HEAs. The review also discusses the mechanical properties, potential industrial applications, and future trends in the field, providing a comprehensive overview of the current state and future prospects of HEA development using powder metallurgy.

Pepper is one of the most important vegetables in South Korea. It is a key ingredient in kimchi, the nation’s staple dish, and serves as the primary raw material for producing gochujang, a commonly used condiment in Korean cooking. As a result, numerous pepper varieties have been developed, including those that yield more fruit or have milder pungency. However, farmers who grow peppers tend to prefer varieties that are resistant to pests and diseases. Bacterial wilt (BW) is one of the most devastating diseases affecting peppers and is transmitted through the soil. To breed pepper varieties resistant to bacterial wilt using molecular breeding techniques, it is essential to first identify the Quantitative Trait Locus (QTL) that confers resistance to this disease. This requires conducting locus analysis with resistant cultivars. In this study, an F2 population was developed by selfing F1 hybrids, which were obtained by crossing a resistant cultivar with a susceptible cultivar, to identify QTLs associated with bacterial wilt resistance. Genotyping-by-Sequencing (GBS) analysis will be performed using the F2 population, and the results will be utilized for QTL mapping.

This study investigated the growth dynamics of Lilium miquelianum bulb scales cultured in four different media formulations: Full Murashige-Skoog (FMS), Half Murashige-Skoog (HMS), Anderson Vitamin (AV), and Knudson Vitamin (KV). Bulb scales were collected from native populations on Jeju Island, Korea, and cultured in vitro for 12 weeks. Growth parameters, including bulb area and scale count, were measured at 3-week intervals. Statistical analyses revealed significant differences in growth rates across media types and time points. FMS consistently demonstrated the highest growth rates and final bulb area and scale count, while KV exhibited the lowest performance. Heatmap analysis showed that FMS achieved the highest weekly growth rates in most time points, with HMS showing comparable performance during early growth stages. Individual sample growth rates varied within media, highlighting the influence of both external and internal factors on growth. Correlation analysis revealed a positive association between bulb size and scale quantity, with FMS exhibiting the strongest relationship. These findings emphasize the importance of appropriate media selection for optimal L. miquelianum propagation, with FMS being the most suitable for extended cultivation. This research provides valuable insights for improving propagation efficiency and conservation efforts of L. miquelianum.

A protruding mass was identified in the papilla of the right kidney of a 10-week-old male Sprague-Dawley rat. Microscopically, the neoplastic tissues were consisted of epithelial elements, where basophilic neoplastic cells displayed a high nucleus-to-cytoplasm ratio and formed tubular growth patterns characterized by small, elongated, or convoluted tubules. Blastemal elements were often arranged in aggregates or nests, composed of tightly packed basophilic polygonal to spindloid primitive cells. The surrounding interstitial tissue appeared loose and myxomatous. Based on these histological features, the diagnosis was nephroblastoma. Nephroblastoma is considered as an embryonic tumor originated from metanephric blastemal elements in the renal cortex and typically displays characteristic triphasic patterns. Also, this tumor seldom arises from or remains localized to the renal pelvis. To our literature review, this is the first nephroblastoma occurred at renal papilla in a rat.

We investigated the stress-induced changes in the lipid and hormonal concentrations in plasma, including cytochrome P450 (CYP)-derived oxidative stress in the liver, and the anti-stress effect of Korean Red Ginseng (KRG) water extract in mice. Stress induction using restraint increased the levels of corticosterone (CORT), glucose, total cholesterol (TC), and low-density lipoprotein-cholesterol (LDL-C) while decreasing in the levels of insulin and high-density lipoprotein-cholesterol (HDL-C), compared with those of unstressed mice. Restraint-stress also increased the generation of reactive oxygen species (ROS) in plasma by 5.4-fold. Moreover, the stress resulted in a 2.8-fold higher production of C-reactive protein (CRP) than the control group. In addition, the catalytic activities of CYP1A2 and CYP3A4 in the liver microsomes were stimulated by 5.5- and 3.8-fold, respectively, and concomitant ROS formation was elevated by 4.3-fold in the liver extract, compared to the normal group. In contrast, the KRG treatment (5, 20, or 50 mg/kg/day) to stress-exposed 3 groups alleviated the increased CORT, TC, LDL-C, ROS, and CRP levels and restored the decreased insulin concentrations. The enhanced each ROS in the plasma and liver, and the CYP enzyme activities were also attenuated in KRG-treated mice in a concentration-dependent manner. In conclusion, these results suggest that KRG ameliorates stress-induced detrimental effects on the plasma and liver of treated mice.

Cell culture is a widely used in vitro tool that enhances our understanding of cell biology, disease mechanisms, drug responses, and the development of tissue engineering. However, there are a number of important drawbacks to conventional two-dimensional (2D) cultures, such as the loss of polarity, altered cell shape, and disruption of cell-extracellular matrix connections. Alternatively, organoids are tissue-engineered, cell-based in vitro models derived from stem cells that can self-organize and differentiate into three-dimensional (3D) structures, recapitulating the morphology and functions of their in vivo counterparts. Bisphenol A (BPA), a ubiquitous industrial chemical, has recently gained recognition as an environmental hazard. Previous research has demonstrated that BPA negatively affects the integrity of the intestinal barrier by triggering programmed cell death and suppressing cell growth in human colonic epithelial cell lines. However, a 2D-based cellular study cannot represent its exposure to multicellular organs. This work investigates the impact of BPA on the structure and function of the intestinal barrier. We examine the effect of BPA on the proliferation and tight junction gene expression with two models: the HT-29 colon cancer cell line and an intestine organoid model and morphological changes of intestinal organoid (I/O). The proliferation was increased in a dose-dependent manner with I/O, but at the same concentration, BPA does not increase the significant number of HT-29 cell respectively. Proliferation-related gene and tight junction gene expression pattern was similar between HT-29 and I/O other than Claudin-4. Therefore, this study offers a more precise depiction of the functional and morphological alterations caused by BPA in comparison to traditional 2D cell cultures.

Polyploidization, or genome doubling, has a significant impact on plant speciation and adaptation, and it is commonly used in agriculture to improve crop traits. In this study, we investigated the induction of polyploidy in three wild Allium species native to Korea: A. senescens and A. spirale Willd. and A. taquetii, using colchicine treatments tailored to meet specific experimental requirements. By avoiding tissue culture methods, we developed a more accessible, cost-effective, and scalable approach to polyploidization. Our research demonstrated that polyploid Allium plants exhibit distinct phenotypic changes, such as reduced growth rates and increased stomatal size. Flow cytometry and chromosome counting confirmed the successful induction of polyploidy, with clear peaks indicating double DNA content and stable chromosome numbers in polyploid plants. The presence of B chromosomes in A. spirale Willd. following polyploidization suggest interesting genetic dynamics. Despite the initial growth lags, polyploid plants may offer enhanced photosynthetic efficiency and resilience under optimal conditions. This study highlights the potential of polyploidization to improve ornamental traits in Allium species, thereby contributing to the diversification and sustainability of ornamental plant offerings. Future research should focus on the long-term performance and ecological adaptability of polyploid Allium species to fully harness their horticultural potential.

The genus Trichophysetis is one of the taxa that has recently undergone frequent changes in its taxonomic position. Only four species have been recorded in Korea, including synonymized Hendecasis species. Recent studies on the taxonomic classification of Trichophysetis have not addressed any species inhabiting Korea. Therefore, it is necessary to conduct a renewed morphological analysis of Korean species. In this study, we compare and analyze four Korean Trichophysetis species with one newly recorded species.

Mocis frugalis (Fabricius, 1775), the sugarcane looper, was collected in Byeonsanbando National Park in South Korea for the first time. This species is known as a major crop pest, especially to sugarcane at tropical and subtropical regions. Due to climate change and the subtropicalization of the southern part of the Korean Peninsula, some tropical and subtropical crops are cultivated in Korea, including sugarcane. Besides, Oryza sativa L. and Zea mays L., which of main crops in Korea, are known as host plants of M. frugalis in the Oriental region. Even if unclear of domestic settlement, precision monitoring will be necessary.

The genus Trichophysetis is one of the taxa that has recently undergone frequent changes in its taxonomic position. Only four species have been recorded in Korea, including synonymized Hendecasis species. Recent studies on the taxonomic classification of Trichophysetis have not addressed any species inhabiting Korea. Therefore, it is necessary to conduct a renewed morphological analysis of Korean species. In this study, we compare and analyze four Korean Trichophysetis species with one newly recorded species.

실감형 콘텐츠 관련 연구가 활발히 진행되고 있다. AR을 중심으로 한 실감형 콘텐츠의 발전은 스마트 폰을 이용하여 실세계에 3D 가상 콘텐츠를 추가하는 것을 넘어, 대형 건축물에 Projection Mapping을 하는 Media Facade 콘텐츠로 발전되었다. 최근 Projection Mapping을 이용한 Media Facade를 활용한 광고 콘텐츠의 수요가 증가하면서 Projection Mapping콘텐츠에 대한 교육과 시스템개발이 요구 되고 있다. Projection Mapping을 처음 배우는 교육생의 경우, 투사할 사물 및 건축물을 만드는 것부터 큰 어려움으로 작용된다. 사물 및 건축물을 구축하기 위해서는 그만한 자원과 재원이 요구되며, 한번 만들고 나면 다시 자원 을 활용하여 바꾸기 쉽지 않다. 본 논문에서는 Projection Mapping 교육을 위한 Unity 3D Engine 기반 Projection Mapping Simulation을 제안한다. 제안된 Projection Mapping Simulation는 Unity 3D Engine을 기반하였기 때문에 무료로 교육생들이 사용이 가능하며, 다양한 3D Object File를 활용하여 사물 및 건축물을 나타내고 조작할 수 있다. 또한, Projection Mapping에 쓰이는 영상을 Video 뿐 아니라, 실시간 3D Rendering으로 사용할 수도 있다. 본 논문에서 제안한 Projection Mapping Simulation는 교육생들에게 공간과 사 물 및 건축물의 이해를 돋기 위한 실험의 장이 될 것이며, Projection Mapping 콘텐츠 연구 자들에게는 여러 Projection Mapping 기법을 실험 및 연구할 수 있는 장이 되길 희망한다.

This study explores the perceptions of Chinese engineering-major students towards their English learning experience after taking a mandatory English composition course in an EMI college. The investigation centers on first-year students’ perceptions of transitioning to EMI, with a focus on how the composition course has assisted students’ transition as a primary language support. Data were collected through a metaphor elicitation technique in which students expressed their perceptions of English learning, complemented by a thematic analysis of 86 reflection essays on the composition class. An analysis of the 334 metaphors identified students’ enhanced confidence in English, the importance of the English-using environment, positive views of learning communities, and increased agency in the learning process. It also revealed that many students were stressed and unaware of learning strategies, therefore suggesting institutional-level language support. One implication is to promote the culture of the learning community. The findings can be particularly useful for programs that are implementing language support for non-English major students.