Gentamicin is an aminoglycoside antibiotic effective against aerobic gram-negative bacteria and is also used in veterinary medicine, particularly in the swine and bovine industries. However, no gentamicin product is currently approved for treating equine diseases in Korea. The present study aims to examine the time-dependent residue of gentamicin in horses after intravenous injection (IV) via jugular vein. The test product was injected at 6.6 mg/kg BW via jugular vein in nine horses. Blood was collected from the horse's jugular vein at 15 minutes, 30 minutes, 1, 4, 8, 12, 24 and 48 hours after injection. To purify the gentamicin in serum, 100μL of 20 mM HFBA in DW, 100 μL of 30% trichloroacetic acid and 300 μL of 20 mM heptafluorobutyric acid (HFBA) in acetonitrile (ACN) were added to 500 μL of serum and supernatant was applied to LC-MS/MS after centrifugation. LC-MS/MS-8050 analyzed the level of gentamicin in serum with Electrospray ionization (ESI) and multiple reaction monitoring (MRM) positive mode. Gentamicin C1 was 478 m/z and product ions were 322, 157 m/z. Precursor ion of Gentamicin C1a was 450 m/z and product ions were 322, 160 m/z. Precursor ion of Gentamicin C2 and C2a was 464 m/z and product ions were 322, 160 m/z. The LC column was a C18 and mobile phase composed of 20 mM HFBA in 5% ACN and 20 mM HFBA in 50% ACN. The amount of gentamicin was calculated by adding four components of gentamicin (C1, C1a, C2 and C2a). The pharmacokinetic parameters of gentamicin were calculated by the WinNonlin program. The Cmax of gentamicin in horse serum was 93 ± 17 μg/kg and the Tmax was 0.25 ± 0 hours. The T1/2 was 6.41 ± 2.32 hours and the CLt was 0.05 ± 0.01L/hr/kg. The Vd was shown as 0.44 ± 0.13 L/kg and the MRT was 1.98 ± 0.55 hours. In conclusion, our data provides useful pharmacokinetic parameters for gentamicin in horses following IV injection.

The swallowing reflex is modulated by multiple sensory inputs, such as bolus volume, viscosity, and taste. The interactions among different types of sensory information have been extensively studied. However, the influence of oral temperature on bolus volume perception has not been investigated. The aim of this study was to assess the effect of temperature on volume perception sensitivity in healthy individuals. Five volumes (5, 10, 15, 20, and 25 mL) of distilled water were estimated at three different temperatures, 4℃ (cold), 21℃ (room temperature), and 45℃ (warm), using a visual analogue scale. There were no significant differences in the sensitivity of oral volume perception across temperatures. These findings suggest that the ability to perceive bolus volume remains stable under temperature variation.

목적 : COVID-19 팬데믹 기간 동안 사회적 거리두기와 비대면 활동 증가로 인해 생활습관이 크게 변화하였다. 본 연구는 근거리 작업시간, 수면시간, 스트레스 수준의 변화가 비정시(근시, 원시, 난시) 발생에 미치는 영향을 분 석하고자 하였다. 방법 : 본 연구는 질병관리청 국민건강영양조사 제8기(2019~2021년도) 조사 중 2020년에 참여한 대상자들 중 만 40세 이상인 성인들을 대상으로 수행된 단면 연구이다. 연구 대상자는 안검진을 받은 성인 중 안과 질환이 없는 2,564명을 포함하였다. 굴절 이상은 등가구면굴절력을 기준으로 하였다. 결과 : 근거리 작업시간이 하루 4시간 이상인 그룹에서 근시 유병률이 유의하게 증가하였다. 반면, 1시간 이하 의 근거리 작업을 수행하는 그룹에서는 원시 유병률이 높았다(p<0.0001). 수면시간이 6~8시간인 그룹에서 근시 유병률이 가장 높았으며, 원시는 6시간 미만 그룹에서 높았다(p=0.0082). 스트레스 수준이 높은 그룹에서 근시 유 병률이 유의하게 증가하는 경향을 보였으나, 다중 로지스틱 회귀분석에서는 근거리 작업시간이 짧은 그룹에서 난시 유병률이 더 높은 경향을 보였으며(p<0.0001), 수면시간과 스트레스의 영향은 통계적으로 유의하지 않았다. 결론 : 본 연구 결과, 근거리 작업시간 증가가 근시 발생에 가장 큰 영향을 미치는 요인임이 확인되었으며, 수면 패턴과 스트레스 또한 비정시와 연관성을 가질 수 있음을 시사하였다. 이는 COVID-19 팬데믹과 같은 환경적 변 화가 시력 건강에 미치는 영향을 이해하고, 근시 예방 및 관리 전략을 수립하는 데 기여할 수 있다.

인공관절 치환술 환자의 합병증 진단을 위한 자기공명영상 검사에서 발생한 금속 인공물을 감소시키는 VAT(view angle tilting) 기법과 딥러닝 알고리즘 중 K-공간 기반의 deep resolve(boost, sharp, DR)를 적용하여 그 유용성 에 대해 평가하고자 하였다. 자체 제작 팬텀과 3T 장비로 일반적인 VAT, DR이 적용된 VAT로 T1 강조영상, T2 강조영상, 단시간 반복 회전 연쇄기법(short tau inversion recovery, STIR) 영상들을 병렬영상 가속계수 2, 3, 4를 적용하여 획득하였다. 획득된 영상에서 왜곡도, 팬텀 바닥에서 금속 인공물까지 거리, 신호대잡음비를 정량적 평가하였고, 영상 품질은 정성적 평가하였다. 왜곡도는 일반적인 VAT와 DR이 적용된 VAT의 T1 강조영상, T2 강 조영상, STIR 모두 유의한 차이가 없었다(p>0.05). 또한 금속 인공물까지 거리도 T1 강조영상, T2 강조영상, STIR 모두 유의한 차이가 없었다(p>0.05). 신호대잡음비는 일반적인 VAT보다 DR이 적용된 VAT의 가속계수 4에서 T1 강조영상은 103%, T2 강조영상은 85.2%, STIR은 73.3% 최대로 증가하였고, 영상품질 평가점수는 T1 강조영상은 5점, T2 강조영상은 4.6점, STIR은 4.8점으로 가장 좋았다. 본 연구를 통해 VAT 기법의 영상품질 저하를 DR 기술 로 개선할 수 있었다. 금속 인공물이 발생했을 때 DR이 적용된 VAT의 가속계수를 4로 적용한다면, 검사시간을 단 축하면서 보다 진단적 가치가 높은 영상을 획득할 수 있을 것으로 사료된다.

산마늘(Allium microdictyon)과 울릉산마늘(A. ulleungense) 은 수선화과(Amaryllidaceae) 부추속(Allium)에 속하는 다년 초 식물로 산마늘은 우리나라에서는 지리산, 오대산 등의 고산 지대, 울릉산마늘은 울릉도에 분포하고 있다. 본 연구는 산마늘 과 울릉산마늘의 휴면과 발아특성을 조사하여 효과적인 대량증 식법을 위한 기초자료를 제공하고자 수행하였다. 실험은 2021 년 7월에 강원도 정선에서 재배한 산마늘 종자와 2021년 8월에 울릉도에서 채종한 종자를 사용하였다. 수분흡수 실험결과, 산 마늘과 울릉산마늘 모두 수분흡수 3시간 만에 20% 이상의 수분 흡수율을 보여 물리적 휴면이 없는 것으로 판단하였다. 온도 처리(25/15, 20/10, 15/6, 5℃) 실험에서 패트리디쉬에 종자 를 치상 후 30일 이전에 5℃를 제외한 나머지 온도 처리에서 모두 발아가 나타났으며, 산마늘과 울릉산마늘 종자의 발아 적 온은 20~25℃인 것으로 확인되었다. 저온층적(0, 4, 8, 12주)처 리 결과, 산마늘과 울릉산마늘 종자 모두 발아율 향상에는 큰 효과가 없었지만, 저온층적처리 기간이 길어질수록 발아세가 증가하는 양상을 보였다. GA3 처리 결과, 산마늘 종자는 처리 농도 간 유의성이 나타나지 않았지만, 울릉산마늘 종자는 처리 농도가 높아질수록 평균발아일수 및 발아균일도에서 유의적인 차이를 나타냈다. 이러한 결과를 통하여 산마늘과 울릉산마늘 종자는 non-deep PD 유형인 것으로 판단된다.

Lumpy Skin Disease (LSD) and Foot-and-Mouth Disease (FMD) cause substantial economic losses on the livestock industry. Therefore, vaccinations have been implemented as the control strategy in endemic countries. However, the potential adverse effects of administering vaccines for both diseases simultaneously have not been thoroughly evaluated. The aim of this study was to assess the impact of vaccinating dairy cows with either or both LSD and FMD vaccines on milk production and physiological parameters such as milk temperature, rumination time and body weight. The experimental groups were divided into four according to the injection materials: 1) saline, 2) LSD vaccine, 3) FMD vaccine, and 4) both vaccines. The impact of vaccination on milk yield and physiological parameters was evaluated daily until 12 days post-vaccination, and milk components were analyzed twice, once per week. Among the experimental groups as well as each vaccine group, no statistically significant differences (p < 0.05) were observed at milk yield, milk components, or milk temperature. This suggests that simultaneous vaccination of LSD and FMD can be administered without adverse effects.

Visfatin, an adipokine secreted by cells, is crucial for intracellular nicotinamide adenine dinucleotide+ biosynthesis. Extracellularly, visfatin plays diverse roles in inflammatory conditions, including obesity, which is closely linked to osteoclastogenesis. We previously showed that visfatin enhances receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclastogenesis in bone marrow-derived macrophages. However, its enzymatic activity during this process is poorly understood. Here, we investigated visfatin’s effects on RANKL-induced osteoclast differentiation. Our results demonstrate that visfatin promotes this differentiation, an effect inhibited by FK866, an inhibitor of visfatin’s enzymatic activity. Furthermore, FK866 also inhibited RANKL-induced osteoclast differentiation. These findings suggest that inhibiting visfatin’s enzymatic activity modulates osteoclast differentiation. Thus, visfatin plays an important role in osteoclastogenesis, both intracellularly and extracellularly, and FK866 has therapeutic potential for diseases characterized by imbalanced osteoclast formation, such as osteoporosis and periodontitis.

This experiment was carried out to evaluate the effect of natural drying methods on the moisture content and germination of Italian ryegrass seed in Jicheon reclaimed land, Jangheung, Korea, from 2023 to 2024. The natural drying methods tested included seed spread thicknesses of 2.5, 5.0 and 7.5 cm; seed reversal frequencies of 1, 2 and 3 times per day; and two reversal methods: seed-only reversal and complete mixing of seed with a dry mat. The thinner seed spread thickness significantly accelerated moisture content reduction(p<0.001). However, the reduction in moisture contents due to the number and method of seed reversal was minimal, approximately 1%. Seeds initially having around 48.9% moisture content required 2, 4 and 5 days to reach a moisture content of 14% when dried at spread thickness of 2.5, 5.0 and 7.5 cm, respectively. Seeds with an initial moisture content of 23-26% required 1 to 2 days to reach 14% moisture content while maintaining approximately 80% germination rate under the tested natural drying methods. When seeds with an initial moisture content of 48.9% were dried at a spread thickness of 2.5 cm, the germination rate was 74.6%, which was significantly lower than the 83.8 and 81.6% germination rates observed for seeds dried at 5.0 and 7.5 cm thickness, respectively(p<0.002). These results suggest that for faster drying and higher-quality Italian ryegrass seed, harvesting at 30% seed moisture content, utilizing the cultivation field as a seed drying place, and applying a seed spread thickness of 2.5 to 5.0 cm are optimal practices.

Effective cooling strategies are critical for cultivating high-quality ornamental plants during the summer. The fan-and-pad cooling system reduces greenhouse temperatures by drawing air through wet pads, which humidify and cool the air, aided by fans on the opposite side. However, the paper-based pads (corrugated cellulose) used in this system have limited durability and degrade with prolonged use. Nanocomposite hydrogels, with their polymer-based structure, can absorb and retain moisture through swelling, presenting a promising alternative. This study examines the application of nanocomposite hydrogels, focusing on their hygroscopic properties and cooling efficiency under various temperatures and wind speeds. When treated with lithium chloride solutions at 25%, 50%, 75%, and 100% saturation, higher LiCl concentrations reduced weight but increased swelling capacity. Optimal cooling effects were achieved with wind speeds of 1.0 m/s at 25°C and 1.5 m/s at 35°C, with greater efficiency observed at lower wind speeds. These findings suggest that integrating nanocomposite hydrogels into cooling pads could enhance durability and reduce maintenance compared with conventional paper pads.

가위벌과에 속하는 Coelioxys의미기록 2종 Coelioxys conoidea Illiger, 1806 와 Coelioxys formosicola Strand, 1913을 한국에서 처음으로 보 고한다. 본 종의 기재, 분포정보, 그리고 삽화를 제공한다.

As part of the 2024 research initiative, “Investigation and Discovery of Prokaryotes in Freshwater Systems,” samples were collected from diverse freshwater habitats, including both water and soil environments. Approximately 2,000 bacterial strains were isolated as single colonies and identified through 16S rRNA gene sequence analysis. Among these, 38 strains shared ≥98.7% 16S rRNA gene sequence similarities with those of known bacterial species not previously reported in Korea. These strains were thus categorized as newly recorded bacterial species in Korea. These 38 bacterial strains displayed significant phylogenetic diversities, spanning 2 phyla, 4 classes, 15 orders, 24 families, and 34 genera. These unrecorded species were classified into the following classes: Actinomycetia (with genera including Microcella, Conyzicola, Curtobacterium, Leucobacter, Microbacterium, Frigoribacterium, Lysinibacter, Streptomyces, Nonomuraea, Actinocorallia, Ruania, and Actinoplanes), Alphaproteobacteria (Paracoccus, Youngimonas, Loktanella, Corticibacterium, Neorhizobium, Onobrychidicola, Ferranicluibacter, Aureimonas, Asticcacaulis, and Novosphingobium), Betaproteobacteria (Rhodoferax, Rugamonas, and Cupriavidus), and Gammaproteobacteria (Rheinheimera, Shewanella, Kosakonia, Leclercia, Hafnia, Yersinia, Pseudomonas, Lysobacter, and Acinetobacter ). Further characterization included assessment of Gram reaction, colony and cell morphology, biochemical properties, and phylogenetic relationships. This report presents detailed phylogenetic and phenotypic characteristics of these bacterial species.

본 연구는 딥러닝 영상 재구성 기법을 적용한 8개의 뇌질환군의 감마나이프 수술 계획용 자기공명영상(magnetic resonance imaging, MRI)의 유용성을 알아보고자 하였다. 연구 방법은 전이성 뇌종양, 뇌동정맥 기형, 수막종, 뇌하수체선종, 삼차신경통, 청신경초종, 맥락얼기 유두종, 해면상 혈관종, 총 8개의 질병을 진단받은 사람들의 T2 강조 영상(T2 weighted imaging, T2WI), 조영증강 T1 강조영상(contrast enhancement T1 weighted imaging, CE-T1WI)의 방법으로 검사한 MRI 영상을 SwiftMR을 이용하여 딥러닝 영상 재구성 기법인 디노이징(denoising)과 초해상도(super resolution)가 적용된 영상을 획득하였다. 이에 대한 성능 평가는 최대 신호대잡음비(peak signal to noise ratio, PSNR), 구조적 유사도(structural similarity index measure, SSIM), 감마나이프 방사선수술(gamma knife radiosurgery, GKRS)의 좌표계로 평가하였다. 그 결과, 원본영상을 기반으로 영상 품질이 개선된 영상의 PSNR과 SSIM은 높은 수치를 나타냄으로써 MRI 영상의 재구성이 문제없이 이루어졌고, GKRS의 수술 좌표계 또한 변화를 보이지 않았다. 결론적으로 딥러닝 영상 재구성 기법은 영상 품질 향상과 영상 보존에서 뛰어난 성능을 보임과 동시에 좌표계도 변화를 보이지 않아서, 딥러닝 영상 재구성 기법은 감마나이프 수술 계획에 유용하게 사용할 수 있는 기법임을 확인하였다.

Periodontitis is a chronic inflammatory disease characterized by the progressive destruction of periodontal tissue and alveolar bone loss. To develop effective treatment strategies, a model that mimics this disease must be implemented. From this perspective, animal models can be used to investigate its mechanisms by reproducing disease progression and providing insights into host-microbe interactions, immune responses, and bone remodeling. In addition, periodontitis-associated bone loss fundamentally differs from systemic bone loss. Targeted treatments require distinguishing periodontitis-induced and systemic bone loss mechanisms. This review examines the rationale for using animal models in periodontal research and evaluates various experimental approaches, such as bacterial inoculation, ligature-induced periodontitis, and chemically induced inflammation. These models have advanced our understanding of periodontal disease but have limitations in replicating the chronic nature of periodontitis and human immune responses. However, current models cannot fully replicate chronic disease progression and human immune responses. Recent developments have focused on improving animal models to more accurately simulate disease progression and host responses, which has led to the elucidation of the immunomodulatory mechanisms of periodontitis and their relevance to the human dental environment. Moreover, new approaches, such as developing age-related periodontitis models and improving ligature techniques, could enhance experimental reproducibility and translational potential. Future studies are needed to reflect these improvements and enhance the clinical relevance of periodontitis models.

This study explored how teachers could provide support to enhance students’ out-ofclass mobile-assisted language learning (MALL) engagement. We interviewed five Korean English teachers who used Class Card, a focal technology of this study, for their students’ self-directed vocabulary learning. Additionally, students of the interviewed teachers completed a survey on their perceptions of teacher support and MALL engagement. This study has three major findings. First, the teachers adopted either a proactive or a passive approach to promoting students’ out-of-class MALL engagement, which was influenced by their beliefs about whether teachers or students should be responsible for learning beyond the classroom. Second, all teachers provided orientation and behavioral support to enhance out-of-class MALL engagement, although the consistency and intensity in providing this support varied between proactive and passive teachers. Finally, students who perceived higher levels of teacher support reported greater out-of-class MALL engagement. We discuss the importance of classroom-based teacher support to enhance MALL engagement beyond the classroom as pedagogical implications.

에듀테크 시대에 접어들면서 디지털 기술을 활용한 학습 방식이 점점 확대되고 있으며, 특히 모바일 기반 애플리케이션 을 활용한 학습이 적극적으로 도입되고 있다. 이러한 학습 방식은 학습자의 참여도를 높이고, 흥미를 유발하며, 학습 효율성 향상에 긍정적인 영향을 미치고 것으로 보고되고 있다. 본 연구는 자기공명영상학 학습에서 모바일 기반 애플리 케이션 사용에 대한 학습자들의 인식, 학습 효과, 학습 만족도를 알아보고자 하였다. 대구시 소재 S 대학교 자기공명영 상학을 수강한 2, 3학년 학생 70명을 대상으로 2024년 11월 24일부터 29일까지 수업 후 모바일 애플리케이션을 활용한 퀴즈 활동을 시행하였다. 연구 결과, 애플리케이션 활용에 대한 학습자들의 인식 평균 점수는 4.58±0.66, 학습 효과는 4.61±0.62, 학습 만족도는 4.58±0.65로 나타났다. 또한, 애플리케이션 활용 전후 비교 분석에서 인식 (활용 전 3.62±0.97, 활용 후 4.58±0.66), 학습 효과(활용 전 3.60±0.92, 활용 후 4.61±0.62), 학습 만족도(활용 전 3.64±0.93, 활용 후 4.58±0.65) 모두 통계적으로 유의한 차이가 있었다(p<0.05). 이러한 결과는 자기공명영상학 교육에서 모바일 애플리케이션 기반 학습이 학습자의 참여도, 이해도, 만족도를 높이는 데 효과적임을 시사한다. 따라서 자기공명영상학뿐만 아니라 다양한 전공 분야에서도 애플리케이션 기반 학습이 유용한 교육 도구로 활용될 수 있으며, 향후 교육 및 임상 실습 현장에서 적용 가능한 기초자료로 활용될 수 있을 것 기대된다.

목적 : 본 연구에서는 회절격자가 광원의 깊이에 따라 회절영상을 주기적으로 생성하는 광학적 특성을 이용하여 중간 범위의 깊이를 측정할 수 있는 시스템을 연구하였다. 방법 : 첫 번째로 회절격자 영상시스템을 기하광학적으로 분석합니다. 두 번째로 회절격자의 주기적인 결상특성 을 파동광학적으로 분석하였다. 세 번째로 주기적인 함수사이의 콘볼루션 특성을 이용하여 깊이에 대응하는 공간주 기를 도출하였다. 네 번째로 1 m에서 4 m까지 1 m마다 타켓을 설치한후 회절격자, 카메라, 레이저로 구성된 회절 격자 영상시스템을 이용하여 회절영상을 획득하는 광학실험을 수행하였다. 다섯 번째로 첫 번째 과정에서 세 번째 과정까지 수행한 이론적 분석을 네 번째 과정에서 획득된 회절영상에 적용하여 깊이측정을 수행하였다. 마지막으로 결과 분석을 통해 시스템의 깊이해상도를 도출하였다. 결과 : 깊이 측정 실험을 수행하였고 제안된 방법의 타당성을 검증하였다. 회절영상배열의 공간주기는 타겟물체 와 회절격자사이의 거리가 멀어질수록 증가한다, 그러나 그 증가하는 비율은 물체거리에 반비례하였다. 결론 : 회절격자의 특성을 응용한 깊이 측정 시스템을 제안하였으며 이론분석과 광학실험을 통해 타당성을 검증 하였다.

Hydrangea is widely grown as an ornamental plant with a high commercial value owing to its appealing features. Although many cultivars exist as genetic resources, they cannot be effectively used for breeding due to insufficient information regarding their genetic relationships and breeding compatibility. Intraspecific and intergeneric hybridizations within the family, Hydrangeaceae present opportunities to create cultivars with desirable traits, however, breeding efforts in Hydrangea through interspecific hybridization have achieved limited success. The low viability of interspecific hybrids may be attributed to cytogenetic incompatibility between the species. Therefore, a deeper cytogenetic understanding of this genus could enhance future breeding programs. This review focuses on the importance of cytogenetic data in the breeding of hydrangeas and the different cytogenetic techniques utilized in this genus. Future directions for cytogenetic research in Hydrangea are also discussed.

본 연구에서 Acyrthosiphon (Acyrthosiphon) lactucae를 국내에서 처음으로 보고한다. 이 종의 무시성충에 대한 형태학적 정보, 분포지역, 기주 식물, 한국에 분포하는 Acyrthosiphon 속 종들의 분류키를 제공한다.

Production technology trials for PARC’s new fodder oat cultivar (PARC-Oat) were conducted at the National Agricultural Research Center (NARC) under rain-fed conditions in Islamabad from 2021 to 2023. The effects of different fertilizer doses, planting densities (seed rates), and inter-row spacing on green fodder yield were studied. The experiment comprised four fertilizer doses of nitrogen and phosphorus (N:P) (55:30, 65:40, 75:50, and 85:60 kg/ha), four seed rate densities (30 kg/ac, 35 kg/ac, 40 kg/ac, and 45 kg/ac), and four inter-row spacings (15 cm, 30 cm, 45 cm, and 60 cm). Results based o n k ey p arameters a ffecting t he y ield of PARC-O at—namely plant height (cm), leaf area (cm²), leaves per tiller, number of tillers per plant, and green fodder yield (t/ha)—indicated that the maximum yield of 72.74 t/ha was observed with the fertilizer dose of 75:50 kg/ha (N:P). Similarly, a seed rate of 40 kg/ha produced optimal planting densities, resulting in the highest green fodder yield of 72.85 t/ha, while an inter-row spacing of 30 cm yielded the maximum green fodder yield of 74.30 t/ha. These results suggest that to achieve maximum green fodder biomass of oats, best management practices should include the application of a fertilizer dose of 75:50 (N:P), a seed rate of 40 kg/ha, and an inter-row spacing of 30 cm.

Mauremys reevesii (Reeves’ turtle) is an endemic freshwater turtle species found throughout East Asia. Due to a rapid population decline, the International Union for Conservation of Nature (IUCN) and the Korean government have classified this species as Endangered (EN). The reported largest population size of M. reevesii in the Republic of Korea was previously estimated to be approximately 20-30 individuals. Our study assessed the population size and structure of M. reevesii at Geumho Reservoir, Republic of Korea, using a capture-recapture data. A total of 433 M. reevesii were incidentally captured during a 35-week trapping process conducted from March to October 2023. The sex ratio of the captured population exhibited a male bias of 1.3 : 1. Sexual size dimorphism was observed only in body weight. Individuals were recaptured up to 11 times during the study period, with males and females being recaptured at an average of 2.1±2.0 times and 1.5±0.9 times, respectively. The estimated population size of M. reevesii in Geumho Reservoir was approximately 891 turtles. The absence of notable sexual size dimorphism and significant sex ratio differences suggests that the population in this area may have been established relatively recently. Compared to previous records, the population in Geumho Reservoir represents the largest single population of M. reevesii, both within the Republic of Korea and globally.