Lentinus giganteus is a edible mushroom cultivated in Asian countries. The present study was initiated to evaluate the anti-inflammatory, anti-acetylcholinesterase, anti-α-glucosidase, and free radical scavenging activities from fruiting bodies of L. giganteus extracted with methanol and hot water. The free radical scavenging activities of methanol and hot water extracts on 1,1-diphenyl-2-picrylhydrazyl (DPPH) were 92.26% and 90.17% at 2.0 mg/mL concentration, respectively and comparable with positive control, BHT. The chelating activities of methanol and hot water extracts were significantly higher than the positive control tested. The reducing power of methanol and hot water extracts showed lower activities compared to positive control, BHT. The phenolic and flavonoid contents of hot water extract were 1.56 μg/mg and 24.35 μg/mg, respectively. Nitric oxide (NO) produced by lipopolysaccahride (LPS) activated RAW 264.7 cells were significantly inhibited by treatment of methanol and hot water extracts. The methanol extract inhibited the acetylcholinesterase (AChE) activity by 91.19% at the 2.0 mg/mL concentration, whereas galanthamine, the standard drug, inhibited the AChE activity by 97.80%. The hot water extracts inhibited the α-amylase and α-glucosidase activities by 78.86% and 80.78%, respectively at the 2.0 mg/mL concentration, while acarbose, the positive control, inhibited the activities by 89.91% and 81.81%, respectively at the same concentration. Therefore, it is concluded that fruiting bodies of L. giganteus contain antioxidant, anti-inflammatory, anti-cholinesterase, and anti-diabetic substances, which can be used for natural health food for promoting human health.
We apply differential affine velocity estimator (DAVE) to the Solar Dynamics Observatory (SDO)/Helioseismic and Magnetic Imager (HMI) 12-min line-of-sight magnetograms, and separately cal- culate the injected magnetic helicity for the leading and the following polarities of nine emerging bipolar active regions (ARs). Comparing magnetic helicity ux of the leading polarity with the following polarity, we nd that six ARs studied in this paper have the following polarity that injected more magnetic helicity ux than that of the leading polarity. We also measure the mean area of each polarity in all the nine ARs, and nd that the compact polarity tend to possess more magnetic helicity ux than the fragmented one. Our results conrm the previous studies on asymmetry of magnetic helicity that emerging bipolar ARs have a polarity preference in injecting magnetic helicity. Based on the changes of unsigned magnetic ux, we divide the emergence process into two evolutionary stages: (1) an increasing stage before the peak ux and (2) a constant or decreasing stage after the peak ux. Obvious changes on magnetic helicity ux can be seen during transition from one stage to another. Seven ARs have one or both polarity that changed the sign of magnetic helicity ux. Additionally, the prevailing polarity of the two ARs, which injects more magnetic helicity, changes form the following polarity to the leading one.
The aim of this study to investigate the effects of craniocervical flexion on muscle activities of scapular upward rotators during push-up plus exercise in subjects with winging scapula. Eighteen males with scapular winging were recruited, and each subject performed knee push-up plus and other exercises, in two conditions (craniocervical flexion vs. natural head positions). A surface electromyography (EMG) was used to measure upper trapezius (UT), serratus anterior (SA), and lower trapezius (LT) muscle activity. A paired t-test was used to determine the statistical significance between the different condition with/without applying of craniocervical flexion. UT EMG activity significantly decreased and SA EMG activity significantly increased during knee push-up plus involving the craniocervical flexion compared to the natural head position. However, no significant differences (p>.05) were found in the activity of the LT muscle. The UT/SA ratios with and without craniocervical flexion showed a significant difference (p<.05). These results showed that the knee push-up plus other exercises performed with craniocervical flexion could strengthen the serratus anterior muscle and minimize the activity of the UT muscle.
Three dimensional self-assembled graphene hydrogels were easily fabricated by electron beam irradiation (EBI) using an aqueous solution of wool/poly(vinyl alcohol) and graphene oxide (GO). After exposure to various levels of EBI radiation, the highly porous, self-assembled, wool-based graphene hydrogels were characterized using scanning electron microscopy and Fourier-transform infrared spectroscopy; to determine the gel fraction, degree of swelling, gel strength, kinetics-of-swelling analyses and removal of hexavalent chromium (Cr(VI)) from the aqueous solution. X-ray diffraction results confirmed that EBI played a significantly important role in reducing GO to graphene. The adsorption equilibrium of Cr(VI) was reached within 80 min and the adsorption capacity was dramatically increased as the acidity of the initial solution was decreased from pH 5 to 2. Changes in ionic strength did not exert much effect on the adsorption behavior.
Conductive polymer composites (CPCs) consist of a polymeric matrix and a conductive fil-er, for example, carbon black, carbon fibers,graphite or carbon nanotubes (CNTs). The criti-cal amount of the electrically conductive fillernecessary to build up a continuous conductive network, and accordingly, to make the material conductive; is referred to as the percolation threshold. From technical and economical viewpoints, it is desirable to decrease the conduc-tive-fillerpercolation-threshold as much as possible. In this study, we investigated the effect of polymer/conductive-fillerinteractions, as well as the processing and morphological devel-opment of low-percolation-threshold (Φc) conductive-polymer composites. The aim of the study was to produce conductive composites containing less multi-walled CNTs (MWCNTs) than required for pure polypropylene (PP) through two approaches: one using various mix-ing methods and the other using immiscible polymer blends. Variants of the conductive PP composite filledwith MWCNT was prepared by dry mixing, melt mixing, mechanofusion, and compression molding. The percolation threshold (Φc) of the MWCNT-PP composites was most successfully lowered using the mechanofusion process than with any other mixing method (2-5 wt%). The mechanofusion process was found to enhance formation of a perco-lation network structure, and to ensure a more uniform state of dispersion in the CPCs. The immiscible-polymer blends were prepared by melt mixing (internal mixer) poly(vinylidene fluoride) (PVD, PP/PVDF, volume ratio 1:1) filled with MWCN.
This study was conducted to determine the oviposition preference and development of adzuki bean weevil, Callosobruchus chinensis (L.) (Coleoptera: Bruchidae) on five different leguminous seeds a) cowpea, b) white kidney bean c) soybean cultivar Daepung d) mung bean and e) azuki bean. A multiple choice test and a nochoice test were conducted to record the number of eggs laid, total developmental time, number of adults emerged, and adult longevity of C. chinensis on the five different leguminous seeds. Both choice and no-choice tests revealed cowpea seed as the most preferred oviposition substrate. Total developmental time from egg to adult stage ranged from 28 to 31 days; shortest of that on the cowpea followed by adzuki and mung bean seeds. Successful development of C. chinensis was not witnessed on soybean or on kidney bean. Rate of adult emergence was higher on adzuki and mung bean seeds with longer adult longevity of those emerged from adzuki bean and cowpea. Higher egg laying preference and shorter developmental time with longer adult longevity of the beetle on cowpea probably signifies importance of physical as well as chemical attributes of the seed as an oviposition substrate other than evolutionary history of the insect. Cowpea seeds can possibly be used as a pull stimulus either as a trap crop or as a stimulant in the mechanical traps against C. chinensis where other leguminous host crops are grown as main crops.
Studies have reported differential attractiveness of yellow, white or blue sticky cards to greenhouse pests, especially to Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) and Trialeurodes vaporariorum Westwood (Homoptera: Alyerodidae). The main aim of this study was to determine whether yellow or blue sticky card offers a better option in monitoring or mass trapping these sympatric greenhouse pests. Laboratory study that included a multiple choice and no-choice tests to observe the attractiveness of blue, yellow, white or green sticky card to F. occidentalis and T. vaporariorum was conducted. Also, a glasshouse study was conducted to determine better composite attractiveness of yellow or blue sticky card. F. occidentalis showed higher preference to blue and yellow sticky cards in laboratory study and blue captured the highest number of F. occidentalis in glasshouse followed by the yellow. However, while the blue sticky card captured few T. vaporariorum, the yellow sticky card captured the highest number of T. vaporariorum in both laboratory and glasshouse tests. Since yellow sticky card received composite affinity of both F. occidentalis and T. vaporariorum, placement of yellow sticky card for the greenhouse pests, rather than blue or yellow targeted to individual species, is recommended for economical and optimal monitoring and/or mass trapping success of the tested greenhouse pests.
왕담배나방 유충은 참깨의 꼬투리를 직접 갉아 먹거나 줄기를 부러뜨려 신초생장을 억제함으로써 수량을 감소시킬 뿐 아니라 종실의 품질도 떨어뜨린다. 2013년에 밀양, 의령, 안동의 노지 및 시설참깨 포장에서 왕담배나방에 의한 참깨의 피해율을 조사한 결과, 밀양, 의령, 안동에서 참깨 꼬투리의 피해율은 각각 노지재배에서 6.2, 11.7, 7.2%였고, 시설재배에서 14.2, 11.9, 4.6%였다. 노지참깨 포장에서 왕담배나방에 대한 요방제 수준을 설정하기 위하여 참깨 줄기의 하부 10∼12마디에 꼬투리가 맺히는 시기와 하부 20∼22마디에 꼬투리가 맺히는 시기에 왕담배나방 3령 유충을 주당 0, 1, 2, 3, 4마리씩 20일간 접종하여 왕담배나방에 의한 수량 감소율과 수량감소율 관련 관계식을 산출하였고, 이 산출식을 이용하여 5%의 수량감소율을 기준으로 요방제 수준을 설정하였다. 줄기하부 10∼12마디에 꼬투리가 맺히는 시기에 왕담배나방의 유충밀도가 주당 1, 2, 3, 4마리일 때 수량감소율은 각각 13.5, 23.0, 33.8, 40.2%였다. 그리고 줄기하부 20∼22마디에 꼬투리가 맺히는 시기에는 각각 4.1, 10.7, 19.5, 29.5%였다. 참깨 생육단계와 왕담배나방의 유충밀도에 따른 수량 감소율과의 관계식은 하부 10∼12마디에 꼬투리가 맺히는 시기가 Y = 10.07x+1.96, R2 = 0.9867, 하부 20∼22마디에 꼬투리가 맺히는 시기가 Y = 7.44X-2.12, R2 = 0.975 이었다. 이 관계식을 이용하여 참깨포장에서 왕담배나방 유충의 요방제 밀도 (5% 수량감소율 기준)를 산출한 결과, 하부 10∼12마디에 꼬투리가 맺히는 시기에는 10주당 3마리, 하부 20∼22마디에 꼬투리가 맺히는 시기는 10주당 10마리였다.
2010년부터 2013년까지 제주, 밀양, 원주의 잡곡포장에 왕담배나방 성페로몬트랩을 설치하여 4월부터 10월까지 유인량을 조사하였다. 지역간 왕담배나방의 평균 발생량은 제주에서 총 329마리로 밀양 및 원주보다 각각 0.5배 및 4.7배 많았다. 왕담배나방의 발생최성기는 지역별 차이가 매우 심하였는데, 제주는 7월 상순~9월 상순, 밀양은 7월 하순∼9월 중순, 원주는 8월 중순∼9월 중순까지로 나타났다. 유효적산온도에 따른 성충의 연간 발생 세대수는 제주 및 밀양이 4세대, 원주가 3세대였다. 1세대 초발생시기는 제주, 밀양, 원주가 각각 5월 3일, 5월 9일, 5월 12일로 제주에서 가장 빨랐다. 반면 2세대는 제주, 밀양, 원주가 각각 7월 11일, 7월 8일, 7월 11일로 밀양에서 가장 빨랐고, 3세대도 제주, 밀양, 원주에서 각각 8월 21일, 8월 18일, 8월 22일로 밀양에서 가장 빨랐다. 4세대는 제주 및 밀양에서 각각 10월 14일, 10월 9일이었다. 왕담배나방 유충의 수수종실 섭식량은 3령, 4령, 5령, 6령에서 각각 5.4개, 12.7개, 31.9개 및 54.2개로 령기가 증가할수록 섭식량도 증가하였다. 왕담배나방 2~3령 유충에 의한 수수 이삭의 피해율은 출수기가 이삭당 유충 1마리 발생시 32.5%, 2마리 62.2%, 3마리 80.8%, 4마리 91.8% 이었고, 개화기는 1마리 21.7%, 2마리 39.8%, 3마리 61.5%, 4마리 80.4% 이었다. 그리고 등숙기는 1마리 10.9%, 2마리 18.6%, 3마리 29.3%, 4마리 46.4% 이었다. 수수 생육단계와 왕담배나방 유충의 발생밀도에 따른 수수의 수량 감소율과의 관계식은 출수기 Y = 23.278X+6.744, R2 = 0.9636, 개화기 Y = 20.197X-0.0712, R2 = 0.999, 등숙기 Y = 11.343X-1.1201, R2 = 0.9807 이었다. 이 관계식을 이용하여 수수포장의 왕담배나방 유충에 대한 요방제 밀도 (5% 수량감소율)를 산출한 결과, 20이삭당 출수기는 1.4마리, 개화기는 5.0마리, 등숙기는 10.8마리였다.
The effect of increased carbon dioxide concentration in atmosphere was examined on the pheromone system of Helicoverpa armigera reared from egg stage to adult in three room. Two of three room (2×2×2 m) were treated with carbon dioxide gas as 600 ppm and 1,000 ppm, respectively. Mean of carbon dioxide concentration was 429.1 ppm in the control, 603.3 ppm for 600 ppm, and 1011.5 ppm for 1,000 ppm during experiment. Electroantenograph (EAG) test was conducted on 3-d-old male adults with air, hexane, and a series of their sex pheromone component, Z11-16Al, from 0.01 to 100 ng. The result was that male EAG responses of 600 and 1,000 ppm were 30.3% lower than that of control room. Production of Z11-16:Al was examined on about twenty 2-d-old virgin females. Carbon dioxide increases did not show a statistically significant difference. However, higher amount of sex pheromone was produced in females of 600 and 1,000 ppm. So, This experiment was replicated with different population reared again. The amount of the sex pheromone per female was 108.9 and 118.1 ng in control room, 139.8 and 141.8 ng in 600 ppm room, and 124.6 and 125.8 ng in 1,000 ppm room.
The purpose of this study was to analysis of the effect of proprioceptor training and vestibular organ training for balance ability. The subjects was consist of two different subjects group, proprioceptor training group and vestibular organ training group. Proprioceptor training group consisted of 10 subjects and vestibular organ training group consisted of 10 subjects. Training was performed 3 times per week, 30 minutes per day, for 3 weeks. Balance ability analysis was performed using Romberg's one leg standing test and BT4 when opened eyes and closed eyes. The analysis results were as follows. There was no significant differences in balance after the training in both groups when they opened their eyes(p<.05). But there was significant differences in balance after the training in both groups when they closed their eyes(p<.05). And there was no significant difference in balance after the training between the proprioceptor training group and the vestibular organ training group when they closed their eyes(p<.05). Given the above results, proprioceptor training and vestibular organ training enhanced balance but there was no significant difference between the two methods.
Carbon nanofibers (CNFs) with diameters in the submicron and nanometer range exhibit high specific surface area, hierarchically porous structure, flexibility, and super strength which allow them to be used in the electrode materials of energy storage devices, and as hybrid-type filler in carbon fiber reinforced plastics and bone tissue scaffold. Unlike catalytic synthesis and other methods, electrospinning of various polymeric precursors followed by stabilization and carbonization has become a straightforward and convenient way to fabricate continuous CNFs. This paper is a comprehensive and brief review on the latest advances made in the development of electrospun CNFs with major focus on the promising applications accomplished by appropriately regulating the microstructural, mechanical, and electrical properties of as-spun CNFs. Additionally, the article describes the various strategies to make a variety of carbon CNFs for energy conversion and storage, catalysis, sensor, adsorption/separation, and biomedical applications. It is envisioned that electrospun CNFs will be the key materials of green science and technology through close collaborations with carbon fibers and carbon nanotubes.
Our previous research on sulfated polysaccharide purified from Ecklonia cava, a brown alga found in Jeju island, Korea, showed that sulfated polysaccharides modulate the apoptotic threshold of intestinal cells, thereby preventing intestinal damage caused by ionizing radiation. In this study, we investigated the ability of sulfated polysaccharide to augment restoration of small intestinal stem cells from γ-ray-induced damage. In our results, sulfated polysaccharide treatment increased the numbers of Ki-67-positive cells as well as inducible nitric oxide synthase (iNOS)-expressing cells in the small intestine compared with those of irradiated only mice. Meanwhile, exposure to irradiation increased the number of paneth cells, which are frequently associated with intestinal inflammation, whereas sulfated polysaccharide treatment reduced the number of paneth cells in the small intestinal crypt. Conclusively, our data suggest that reduction of iNOS-expressing cells and paneth cells in sulfated polysaccharide-treated mice contributes to the inhibition of radiation-induced intestinal inflammation.
In this study, characteristics and immuno-modulatory effects of Weissella cibaria JW15 isolated from Kimchi, traditional Korean fermented food, were examined for investigation of the capacity of potentially probiotic strains. We measured acid, bile, and heat tolerance, adhesive properties to intestinal epithelial cells, and inhibitory activity against pathogens. JW15 could survive at pH 3.0 for 2 hr, but not at pH 2.0. JW15 also showed tolerance to 0.3% oxgall bile salt, and heat tolerance at 70°C and 80°C for 5 min, respectively. Adhesive ability to Caco-2 cells was similar to that of Lactobacillus rhamnosus GG (LGG), a well-known commercial probiotic. JW15 exhibited antimicrobial activities to pathogenic bacteria such as Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, and Salmonella enteritidis. The immuno-modulatory effects of JW15 were compared with those of LGG, a well-known immune enhancer. For analysis, production of nitric oxide (NO), NF-κB (Nuclear factor κB), Interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) was measured. The concentration of NO induced by JW15 was higher than that by LGG at low concentration (1 × 107 cfu/mL). Low and high (5 × 107 CFU/mL) concentration of JW15 induced statistically higher production of NF-κB, IL-1β, and TNF-α than that produced by LGG, respectively. In conclusion, Weissella cibaria JW15 had ability as a probiotic strain, including acid, bile, and heat tolerance, adhesive properties to intestinal epithelial cells, and inhibitory activity against pathogens. In addition, JW15 showed better immuno-modulatory effects than LGG when NO, NF-κB, IL-1β, and TNF-α were measured. According to these results, the characteristics and immunomodulating activity of Weissella cibaria JW15 are suitable for consideration as a potential probiotic.
Salmonellosis is one of the life-threating diseases of goat in Bangladesh. Therefore, the present study was designed to study the prevalence of Salmonellosis, and isolation and characterizations of the Salmonella spp. from apparently healthy and diarrheic goat. A total of 47 faces samples were collected from selected place and cultured onto different prescribed medium to isolate it. In this study, 12.76% (6/47) samples were found to be positive for Salmonella spp. During culture on SS agar medium, all of the Salmonella isolates produced round, smooth, opaque, translucent and black color colonies on SS agar media. All of the isolated Salmonella spp. fermented dextrose, maltose and mannitol with production of acid and gas but did not ferment sucrose and lactose. However, these isolates had showed Indole and Voges-Proskauer test negative, Methyl-Red test positive. All of these isolates were subjected to rapid plate agglutination test with polyvalent “O” (Poly ‘O’) and polyvalent “H” (poly ‘H’) antisera where positive agglutination were observed. They were highly sensitive to ciprofloxacin, spiramycin and gentamycin; moderately sensitive to oxytetracyline, streptomycin and amoxicillin; less sensitive to sulphamethoxazole and resistant to penicillin-G. Based on the present findings, it may be concluded that the investigated Salmonella spp. from goats might be S. typhimurium, S. enteritidis, S. brandenburg, S. salford, S. newbrunswick, S. newport or S. dublin. Further study will be needed, therefore it requires further characterization using other serological and molecular techniques.
Polyporus umbellatus (Syn. Grifola umbellata) is a sclerotium forming mushroom belongs to family Poly-poraceae of Polyphorales, Basidiomycota. The sclerotia of P. umbellatus have long been used for traditional medicinesin China, Korea and Japan. This study was initiated to obtain the basic data for artificial sclerotial production of P. umbel-latus. Here, we investigated the favorable conditions for mycelial growth of P. umbellatus and its symbiotic fungus Armill-aria mellea. We also evaluate the favorable carbon and nitrogen sources for sclerotial formation in dual culture betweenP. umbellatus and A. mellea. The favorable conditions for mycelial growth of P. umbellatus were 20°C and pH 4, whileoptimal conditions for mycelial growth of A. mellea were 25°C and pH 6. The carbon sources for optimal mycelial growthof P. umbellatus were fructose and glucose, while carbon sources for favorable mycelial growth of A. mellea were alsofructose and glucose. The nitrogen sources for favorable mycelial growth P. umbellatus were peptone and yeast extract,while optimal mycelial growth of A. mellea were obtained in peptone and yeast extract. When P. umbellatus and A. melleawere dual cultured on carbon sources, sclerotia were induced on basal media supplemented with glucose, fructose andmaltose at pH 4~6, while nitrogen sources inducing sclerotia were basal media supplemented with peptone and yeastextract for 60 days at 20°C under dark condition.