Phytophthora capsici Leonian causes root rot and stem blight in pepper (Capsicum spp.) and is a serious threat to pepper production because of its ability to infect every root, stem, and leaf at any developmental stage. Recently, pepper F1 cultivars resistant to Phytophthora root rot have been commercially released in Korea. However, despite many studies, the inheritance of resistance remains controversial due to differences in experimental methods, including pepper materials, pathogen isolates, inoculation conditions, and evaluation methods. Our aim was to determine the inheritance of Phytophthora root rot resistance by using three different F2 populations derived from crosses between ‘CM334’ (a resistant male parent) and three Korean landraces, ‘Subicho’ ‘Daehwacho’ and ‘Chilsungcho’ (susceptible female parents), and inoculating them with three different pathogen densities (1 ¯ 10 4 , 1 ¯ 105 , and 1 ¯ 106 zoospores/ml). The distribution patterns were varied, depending upon female parental susceptibility as well as inoculum densities. For example, as the inoculum density increased, pepper survival rates decreased. In all of the inheritance analyses, one common dominant resistant gene was participated in resistance to Phytophthora root rot. In addition, we found that a complementary gene, together with the major dominant gene, was necessary for resistance at a high (10 6 ) inoculum density, based on a 9:7 (R:S) segregation ratio. This study will be helpful in developing molecular markers linked to genes that are resistant to Phytophthora root rot.

Regarding carotenoids content, the genetic basis, heritability and combining ability in six red pepper inbred lines were investigated using full diallel crosses. Both additive and non-additive gene actions govern inheritance of carotenoids content. The mean square of array through variance and covariance analysis (Wr-Vr) was insignificant, which suggest that inbred lines involved in diallel cross may have no epistatic effects. The Vr/Wr graph revealed the influence of partial dominant gene action towards low carotenoids content and the absence of non-allelic interaction. The H2 component was smaller than the H1 and the [H2/4H1] component was 0.187 less than 0.25, indicating unequal proportion of positive and negative alleles in the parents. The estimates of broad and narrow sense heritability for carotenoids content were 0.956 and 0.832, respectively. The variance of general combining ability (GCA) was relatively higher than that of specific combining ability (SCA), which implied that the additive gene effects were predominant as compared to both dominant and epistatic effects for the accumulation of carotenoids in this genetic population. The values of GCA of ‘62024L1’ and ‘62067L2’ were higher than those of the other parents. These 2 inbred lines, therefore, can be considered as useful breeding materials to enhance fruit carotenoids content in other red pepper varieties.

This study was done to characterize the anther derived regenerants (R1) including haploids and spontaneous diploids of minipaprika (cvs.‘Vine sweet-red’ ‘Vine sweet-yellow’ and ‘Vine sweet-orange’) in glasshouse. Eleven haploids (three, seven and one from red, yellow and orange, respectively) and sixteen spontaneous diploids (five, nine and two from red, yellow and orange, respectively) were grown in plastic pot with three (red, yellow and orange) anther donor (R0) F1minipaprika varieties. Regenerants were characterized for their plant and fruit characters as well as their fruit color and shape. The homozygosity of spontaneous diploid plants of each population was assessed using simple-sequence repeat (SSR) marker analysis. Haploid plants were characterized by reduced plant height, small leaves, short petiole and internode and small flower bud and all haploids showed the sterility and vice-versa in spontaneous diploid lines. The fruit biometrical traits exhibited greater variation within the spontaneous diploid plants and average value of quantitative traits is lower than standard varieties. MR-4 gave the highest yield (150.5 g) per plant followed by MY-6 (140.0 g) and MY-8 (130.5 g) and the lowest in MY-5 (31.5 g). Morphological marker such as fruit color further determinedthe microspore origin of androgenic diploids obtained in anther culture of ‘Vine sweet-red’. Of the fifteen spontaneous diploid plants, fourteen plants were identified as doubled haploids using microsatellite markers (SSR), and these homozygous lines are recommended to use in minipaprika breeding program.

전해정련을 이용한 폐 피복관 처리의 타당성을 살펴보기 위하여, 500℃의 LiCl-KCl 용융염 내에서 표면이 산화된 10 g 규모의Zircaloy-4 피복관 및 순수한 Zircaloy-4 피복관의 전기화학적 거동을 살펴보았다. 산화된 Zircaloy-4 피복관이 담긴 basket을 작업전극으로하여 전압-전류 관계를 측정한 결과, 산화되지 않은 Zircaloy-4 피복관과 비교해 Zr의 산화 peak는 Ag/AgCl 기준전극 대비, 약 -0.7 V ~ -0.8 V로 유사한 반면, 산화 전류의 크기는 확연하게 감소하는 것으로 나타난다. 이러한 결과는 -0.78V의 일정전위를 가한 전기화학적 용해 실험에서 살펴본 전류-시간 곡선에서도 유사하게 나타나며, 피복관 조각들의 평균 두께 및 무게 변화로부터 확인할 수 있다. Zircaloy-4 피복관이 산화되었을 경우, 표면의 산화막이 피복관의 전도성에 영향을 주어 basket 내 위치에 따라 전기화학적 용해의 균일성 및 속도를 떨어뜨리는 것으로 나타나지만, 표면의 미세한 결함과 Zr 산화물의 상 특성으로 인해 전기화학적 용해가 진행되는 것으로 판단된다.

This study was carried out in order to catalogue the folk plants of 7 counties and cities of northern region of Chungcheongbuk-do from March to October, 2011. Based on the 626 survey sheets collected from 67 residents at 17 places of 7 counties and were subsequently analysed. The identified folk plants in the northern region of Chungcheongbuk-do consisted of a total 348 taxa; 98 families, 250 genera, 298 species, 5 subspecies, 38 varieties, and 7 forms. The use by its usage were: 223 taxa; edible, 123 taxa; medicinal, 4 taxa; dye, 2 taxa; aroma, 6 taxa; spice, 32 taxa; ornamental, 11 taxa; oil, 4 taxa; starch, 22 taxa; and others, respectively, so the edible use is the highest. The most useful part was the leaf, followed by fruit and root. The consistency comparison between the scientific and the local name were the highest in the 50's and the lowest in 80's.

Herb extracts commercially used in Korea were screened for PPAR-γ agonist test and α-glucosidase inhibition assay. Total 16 herb plants had a PPAR-γ agonist activity. Specially, Alisma orientale Juz (108.41%), Ephedra sinica (98.22%), Sasa japonica Makino var. purpurascens Nakai (140.68%), Astragalus membranaceus Bunge (106.79%) and Cnidium officinale Makino (113.00%) showed high PPAR-γ agonist activity rate compared with rosiglitazone's (167.46%). And Cornus officinalis S. et Z. (90.3%), Cinnamomum cassia Blume (89.2%), Psoralea corylifolia L. (89.8%), Paeonia japonica (Makino) Miyabe (92.4%) and Paeonia suffruticosa Andr (93.2%), showed high α-glucosidase inhibition rates. These results support previous reports of the efficacy of Oriental medicinal plants used for diabetes mellitus.

Muscle strength and endurance activities of Korean ginseng (Panax ginseng C. A. Meyer; KG) were compared with those of wild simulated cultivation ginseng (WCG) in mice. Fifty male ICR mice were divided into five groups: A (vehicle); B (WCG 100 mg/kg); C (WCG 500 mg/kg); D (KG 100 mg/kg); E (KG 500 mg/kg). Subsequently, the mice were subjected to the forced swimming test (FST) and treadmill test at the 4th and 7th weeks. The glycogen content in the muscle and blood analysis (levels of glucose, triglyceride (TG), IGF-1) were also performed immediately after the last FST and treadmill test at the 7th week. Immobility times in FST were shorter in WCG- than KG-treated groups, and the results of the treadmill tests were also significant except for KG-treated at 100 mg/kg. The glycogen content was increased in both groups with a peak at 500 mg/kg of WCG groups. Serum concentrations of TG and glucose were decreased by administration of KG and WCG and all treated groups showed increase in the level of IGF-1 in serum. These results suggest that KG and WCG supplementations are effective in escalating the muscle strength and endurance.

To date, carbon and nitrogen co-doped photocatalysts (CN-TiO2) for environmental application focused mainly on the aqueous phase to investigate the decomposition of water pollutants. Accordingly, the present study explored the photocatalytic performance of CN-TiO2 photocatalysts for the purification of indoor-level gas-phase aromatic species under different operational conditions. The characteristics of prepared photocatalysts were investigated using X-ray diffraction, scanning emission microscope, diffuse reflectance UV-VIS-NIR analysis, and Fourier transform infrared (FTIR) analysis. In most cases, the decomposition efficiency for the target compounds exhibited a decreasing trend as input concentration (IC) increased. Specifically, the average decomposition efficiencies for benzene, toluene, ethyl benzene, and xylene (BTEX) over a 3-h process decreased from 29% to close to zero, 80 to 5%, 95 to 19%, and 99 to 32%, respectively, as the IC increased from 0.1 to 2.0 ppm. The decomposition efficiencies obtained from the CN-TiO2 photocatalytic system were higher than those of the TiO2 system. As relative humidity (RH) increased from 20 to 95%, the decomposition efficiencies for BTEX decreased from 39 to 5%, 97 to 59%, 100 to 87%, and 100 to 92%, respectively. In addition, as the stream flow rates (SFRs) decreased from 3.0 to 1.0 L min-1, the average efficiencies for BTEX increased from 0 to 58%, 63 to 100%, 69 to 100%, and 68 to 100%, respectively. Taken together, these findings suggest that three (IC, RH, and SFR) should be considered for better BTEX decomposition efficiencies when applying CN-TiO2 photocatalytic technology to purification of indoor air BTEX.

To investigate the involvement of prepro-proopiomelanocortins (POMCs) with hypermelanosi on the blind side of olive flounder, Paralichthys olivaceus, we surveyed tissue sites in which the three POMCs genes are expressed, and compared pituitary-ofPOMCs (ofPOMC-Ⅰ, Ⅱ and Ⅲ) mRNA activities between the normal and the hypermelano flounders. For the studies, we refereed to the information of ofPOMC-Ⅰ and Ⅱ genes in NCBI, but we try to newly discover ofPOMC-Ⅲ sequence. At first, we cloned and characterized cDNA encoding its ofPOMC-Ⅲ from the pituitary of olive flounder The ofPOMC-Ⅲ cDNA consists of 1,258 bp nucleotides and 7 peptides (Signal peptide, N-POMC, α-MSH, CLIP, N-β-LPH, β-MSH and β-END-like peptide) encoded in 231 amino acids. In the test of tissue distribution of ofPOMCs in olive flounder, we found that ofPOMC-Ⅰ is expressed in pitutary only, but ofPOMC-Ⅱ and Ⅲ are expressed in all tissues examined. In the comparison of ofPOMCs mRNA expression between the normal and the hypermelano fish, expression of ofPOMC-Ⅰ, Ⅱ mRNA was significantly higher in the hypermelano group than in normal group, but ofPOMC-Ⅲ was not significantly different between two groups.

PCR-based assays with co-dominant or dominant allele-specific STS (sequence tagged sites) markers and sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) have been used in this study to discriminate Glu-1 and Glu-3 alleles in Korean wheat cultivars. Three alleles were identified at each high molecular weight glutenin (Glu-1) homoeologous locus and 10 alleles were identified at low molecular weight glutenin (Glu-3) homoeologous locus in Korean wheat cultivars. At Glu-1 loci, wheat cultivars contained Glu-A1a, b and c alleles amplified a 1093, 1063 and 920bp fragments, respectively, Glu-B1b, c and f alleles discriminated with 707(Glu-B1b and f) and 662bp(Glu-B1c) fragments and Glu-D1a, d and f alleles amplified a 576(Glu-D1d) and 612bp(Glu-D1a and f) fragments, respectively. At Glu-3 loci, wheat cultivars contained Glu-A3c, d and e alleles amplified a 573, 967 and 158bp fragments, respectively. Glu-B3d, g, h and i alleles gave a 662, 853, 1022 and 621bp, respectively and Glu-D3a, b and c alleles amplified a 884(Glu-D3a and b) and 338bp(Glu-D3c) fragments. A multiplex-PCR assay was also established which permitted the discrimination of the major Glu-1 and Glu-3 to determine glutenin compositions in a single PCR reaction and agarose gel assay because these systems could be useful to select DNA-based identification of wheat lines carrying good breadmaking performance in Korean wheat breeding programs. Three multiplex-PCR for Glu-1 and Glu-3, Glu-A1c + Glu-B1acf + Glu-D1d, Glu-A3ac + Glu-A3d + Glu-A3e and Glu-B3d + Glu-B3fg + Glu-B3h were established in this study.

Fusarium head blight (FHB), caused by Fusarium graminearum is a major disease problem on wheat and barley in Korea and around the world. We screened for Type II resistance in the greenhouse using single floret inoculation and for Type I resistance in the field using spray inoculation. Sumai 3 was used the FHB resistant check. Three hundred and seventy lines were evaluated for resistance to spread of symptoms within spike (type II). The 2012 field screening with 300 wheat lines was located in Kimjae-si Joeonbuk Korea. All plots were inoculated twice. The first inoculation was applied at anthesis for wheat. The second inoculation was applied three days after the initial inoculation (dai) for each plot. The inoculum was F. graminearum (GZ3639) prepared at a concentration of 100,000 macroconidai/ml with Tween 20 added as a wetting agent. Mist-irrigation was applied from the first inoculation on May 7 till June 7 to facilitate FHB development. FHB severity was assessed visually 21 days after inoculation on 20 arbitrarily selected spikes per plot. FHB severity was determined as the percentage of symptomatic spikelets from the total of all spikelets observed in these 20 spikes. Based on the field test, we could observe four categories of FHB severity: resistant (R: 0-20%), moderately resistant (MR: 21-40%), moderately susceptible (MS: 41-60%), and susceptible (S: 61-100%). The results showed that forty four lines showed the resistant category on FHB severity between 2.7% and 19.8%. In addition, ten lines showed similar FHB severity compared to Sumai 3 (9.9%).

Rice is one of the most important major food crops which provide the major food for more than half of global population. To improve the grain quality as well as grain yield has been the essential breeding goal in rice. The composition of amylopectin is the determinant of rice eating quality under certain threshold of protein content and the ratio of amylose and amylopectin. In this study, RBE 1 driven by CaMV-35S promoter was constructed and transformed using Agrobacterium tumefaciens. We selected single copy with low amylose content among transgenic lines. The mRNA expression was investigated using RT-PCR, and enzyme activity was determined using activity staining method in mid-milky stage endosperm. Also, the overexpression vectors for RBE 1 and SSS 1 driven by seed specific globulin promoter were constructed, respectively. Moreover, the RNA interference vectors for soluble starch synthase 1 and granule bound starch synthase 1 derived by CaMV35S promoter were constructed, respectively and transformed using Agrobacterium tumefaciens. The transgene has been confirmed by amplification of HPT and target gene. The transgenic plants obtained will be used to investigate the gene function of related starch pathway in plant cells using Gopumbyeo as a wild type rice, based on the gain-of-function and the loss-of-function. The development of designed site-specific endonucleases boosted the establishment of gene targeting (GT) techniques in a row of different species. However, the methods described in plants require a highly efficient transformation and regeneration procedure and, therefore, can be applied to very few species. Here, we describe a highly efficient GT system that is suitable for all transformable plants regardless of transformation efficiency. Efficient in planta GT was achieved in rice by expression of a site specific endonuclease (SSS1::ZFN) that not only cuts within the target but also the chromosomal transgenic donor, leading to an excised targeting vector.

The purposes of this research project are to identify quantitative trait loci (QTLs) associated with yield-related traits using a recombinant inbred line (RIL) population derived from a cross between a high-yield soybean genotype SS0404-T5-76 and Daewonkong and to develop high-yield soybean and lodging-resistant sprout soybean cultivars. For development of DNA markers and identification of functional sequence variations, firstly, whole genome of five soybean genotypes, Sinpaldalkong 2, SS2-2, Pungsanamulkong, SS0404-T5-76 and Daewongkong, were sequenced using Illumina Hi-Seq technology. SS2-2 is a EMS-induced mutant of Sinpadalkong 2. SS0404-T5-76 showing high-yield is a F8 RIL derived from a cross of Pungsanamulkong x SS2-2. Daewonkong is a elite cultivar with high-protein. Furthermore, to construct a genetic linkage map, we are advancing F4 lines of SS0404-T5-76 x Daewonkong by single seed-descent. Secondly, we developed high-protein and high-yield soybean lines and lodging-resistant sprout lines. Area-adaptability tests of these promising lines are performing in three different locations including Jeju, Naju, and Suwon. Based on the results of area adaptability tests, we are planing to conduct cultivar registration of the promising soybean lines.

In pepper, investigation of important traits such as disease resistances, high yield and pungency were mostly focused on a cultivated species, Capsicum annuum. This narrow breeding pool hampered to develop improved cultivars. Exploitation of wild germplasm in Capsicum has been recognized as an important issue. The construction of core collection and analysis of genetic diversity in Capsicum is the first step to make full use of germplasm. Although there have been several attempts to construct core collections in Capsicum, most of the works were limited due to handling small number of samples, relying mainly on the characterization of morphological traits and focusing on C. annuum species. Therefore, the comprehensive studies for genetic diversity and structure of Capsicum including phenotypic data, molecular marker patterns and evaluation of useful alleles are very necessary to understand the structure and patterns of genetic diversity in Capsicum. We are developing for a core collection set in Capsicum using molecular markers and phenotypic data with over 3,000 germplasm accessions.

Cucumber mosaic virus (CMV), which has the broadest host range among plant viruses, is a very destructive pathogen in pepper production. Various resistance sources against CMV have been identified by plant breeders. One of them is Capsicum annuum ‘Bukang’, which contains a single dominant resistance gene, Cmr1. C. annuum ‘Bukang’ is resistant to CMV-P0 strains (CMV-Kor and CMV-Fny), but susceptible to CMV-P1. CMV-P1 is a new strain recently identified in South Korea. Previously we showed that CMV-P1 RNA1 helicase domain is responsible for overcoming Cmr1 and may be play a role in viral replication and systemic infection. To identify the plant host factors involved in CMV-P1 replication and movement, we utilized a yeast two-hybrid system derived from C. annuum ‘Bukang’ cDNA library as a prey. A total of 78 potential host genes interacting with the CMV-P1 RNA1 helicase domain were isolated in the first screening, and PCR confirmation and sequencing analysis narrowed the candidates to ten genes. The candidate genes have found to be encoding acireductone dioxygenase, ADP-glucose pyrophosphorylase, ADP-ribosylation factor 1, ADP-ribosylation factor, calreticulin-3 precursor, cysteine synthase, formate dehydrogenase, histone-H3, phosphomannomutase and polyubiqutin 6PU11. Previous studies showed that these genes were involved in virus infection, replication or virus movement. To elucidate the function of these genes, VIGS and coimmumo precipitation assay is being done.

The latest report on draft genome of Brassica rapa sequence has been published. To elucidate the functions of a large population of these genes and to search efficiently for agriculturally useful genes, the Full-length cDNA Over-eXpressor (FOX) gene hunting system was used. The FOX library was transformed into rice by Agrobacteriummediated transformation. Approximately 1,150 FOX-rice lines were generated. Genomic PCR analysis indicated that the average length of FL-cDNAs was 900∼1,200 bp with functional annotation of many unknown function (35.5%). Most of the randomly selected transgenic rice lines showed overexpression (92%) and barely mRNA expression in the wild type Gopum. Moreover, 94% of the 850 transgenic rice lines were moderately tolerant (slightly yellow) to cold and 9 lines were tolerant (seedling light green). For the salinity evaluation, most of the transgenic lines (85%) were highly susceptible whereas seven lines were tolerant. In addition, morphological evaluation of rice lines showed minimal phenotypic alteration (12%). About 25.1 and 22% were earlier in terms of days to heading and chlorophyll contents, respectively. Further, 18% of FOX rice lines showed lower chlorophyll contents. Filled grains, number of tillers, panicle length, culm and plant height were relatively less variable among the lines. These results provided useful genes for functional analyses in the mechanisms of identified transgenic tolerant lines.

The Cmr1 gene in peppers confers resistance to Cucumber mosaic virus isolate-P0 (CMV-P0). Cmr1 restricts the systemic spread of CMV-Fny, whereas this gene cannot block the spread of CMV-P1 to the upper leaves, resulting in systemic infection. To identify the virulence determinant of CMV-P1, six reassortant viruses and six chimeric viruses derived from CMV-Fny and CMV-P1 cDNA clones were used. Our results demonstrate that the helicase domain encoded by CMV-P1 RNA1 determines susceptibility to systemic infection. To identify the key amino acids determining systemic infection with CMV-P1, we then constructed amino acid substitution mutants. Of the mutants tested, amino acid residues at positions 865, 896, 957, and 980 in the 1a protein sequence of CMV-P1 affected the systemic infection. Virus localization studies with CMV-GFP clones and in situ localization of virus RNA revealed that these four amino acid residues together form the movement determinant for CMV-P1 movement from the epidermal cell layer to mesophyll cell layers. Quantitative real-time PCR revealed that CMV-P1 and a chimeric virus with four amino acid residues of CMV-P1 accumulated more genomic RNA in inoculated leaves than did CMV-Fny, indicating that those four amino acids are also involved in virus replication. These results demonstrate that the helicase domain is responsible for systemic infection by controlling virus replication and cell-to-cell movement. Whereas four amino acids are responsible for acquiring virulence in CMV-Fny, six amino acid (positions at 865, 896, 901, 957, 980 and 993) substitutions in CMV-P1 were required for complete loss of virulence in ‘Bukang’.

eIF4E family is well known for recessive resistance gene of potyvirus in many crops. And Turnip mosaic virus (TuMV) is one of the major viruses in Brassicaceae crops which belong to the genus Potyvirus. To elucidate the key amino acids in the interaction between TuMV VPg and Brassica eIF(iso)4E, amino acids of eIF(iso)4E were mutated. Seven amino acids in cap binding pocket were chose for the candidate amino acid that may play a role in the interaction of TuMV VPg. We demonstrated that a single amino acid mutation in cap binding pocket of Brassica eIF(iso)4E can abolish the interaction with TuMV VPg. eIF(iso)4E which has a mutation at each W49, W95 and K150 positions impaired in its interaction with VPg prominently according to the yeast two hybrid analysis. Complementation of an eIF4E knockout yeast strain by mutated eIF(iso)4E proteins showed that all eIF(iso)4E mutants were able to complement eIF4E of yeast. To find out if these mutations affect the susceptibility of Chinese cabbage, transformant analysis was performed. eIF(iso)4E W95L, W95L/K150E and susceptible wild type were over-expressed in susceptible Chinese cabbage. According to the TuMV screening result of T1 and T2 transformants, over-expression of the eIF(iso)4E mutants showed resistance to four TuMV strains (CHN2, 3, 4 and 5). Our results support that the mutations in eIF(iso)4E may control the broad spectrum TuMV resistance.

The antimicrobial peptide possesses defence system to virus, fungi and bacteria. To study antibiotic in plant, antimicrobial peptides were obtained by PCR analysis by primers designed from antimicrobial peptides (Gene bank accession no. NM-004345), cloned in pET28 expression vector and the vector transformed into E. coli. And this gene was inserted into Ti-plasmid VB2 vector, which contained the pGD1 promoter. The expression construction was transformed into Agrobacterium EHA105 and then plant tissues of rice (Oryza sativa). Seeds from transgenic plants (T0) were germinated on selective media containing spectinomycin 50 mg/L. Selected plants and wild type were analyzed by PCR and RT-PCR with pGD1 promoter region and transgene specific primer set. All transgenic plants showed expression pattern of similar levels. We showed that the chromobody is effective in binding GFPand antimicrobial peptide gene in tobacco leaf. Most interestingly, this can be applied to interfere with the function of GFP fusion protein and to mislocalize (trap) GFP fusions to the plant cytoplasm in order to alter the phenotype mediated by the targeted proteins. Bacterial blight disease was enhanced resistance in transgenic lines. These results showed that antibiotic peptides might show a broad-spectrum antimicrobial activity.

Kenaf (Hibiscus cannabinus) is an annual herbaceous plant of the family Malvaceae that has been planted in Africa for more than 4000 years and used as source of fiber, energy and feed stock. Also, kenaf seeds are good source for edible oil used for first class cooking oil and margarine production. The seeds can be used for lubrication, soap, paint and varnishes. This study was carried out to evaluate fatty acids variation among sixteen kenaf germplasm and gamma-ray induced mutants derived from Jinju and Auxu. Linoleic, oleic, and palmitic acid were the predominant fatty acids in all kenaf seed oils. The sixteen accessions showed a wide range of fatty acid compositions, spanning from 28.94 to 43.36% saturated, 56.64 to 71.05% total unsaturated, 15.52 to 46.85% monounsaturated, and 13.56 to 48.97% polyunsaturated fatty acids. The mutant lines derived from Jinju, significantly surpassed parental mean for all the palmitic and oleic acid. Also, the mutant lines derived from Auxu showed broad ranges of variation in oleic and linoleic acid and narrow ranges of variation in stearic and palmitic acid. The relative amount of monounsaturated fatty acids (MUFA) were increased at all the gamma-ray induced mutants. These results will provide a valuable information to assist parental selection of kenaf breeding.