Increasing the efficiency of HR (homologous recombination) is important for a successful knock-in. Rad51 is mainly involved in homologous recombination and is associated with strand invasion. The HR-related mismatch repair system maintains HR fidelity by heteroduplex rejection and repair. Therefore, the purpose of this study is to control Rad51, which plays a critical role in HR, through UV-induced DNA damage. It is also to confirm the effect on the expression of MMR related genes (Msh2, Msh3, Msh6, Mlh1, Pms2) and HR-related genes closely related to HR through treatment with the MMR inhibitor CdCl2. The mRNA expression of Rad51 gene was confirmed in both HC11 cells and mouse testes, but the mRNA expression of Dmc1 gene was confirmed only in mouse testes. The protein expression of Rad51 and Dmc1 gene increased in UV-irradiated HC11 cells. After 72 hours of treatment with 1 μm of CdCl2, the mRNA expression level of Msh3, Pms2, and Rad51 decreased, but the mRNA expression level of Msh6 and Mlh1 increased in HC11 cells. There was no significant difference in Msh2 mRNA expression between CdCl2 untreated-group and the 72 hours treated group. In conclusion, HR-related gene (Rad51) was increased by UV-induced DNA damage. Treatment of the MMR inhibitor CdCl2 in HC11 cells decreased the mRNA expression of Rad51.

Ornamental chrysanthemum (Dendranthema × grandiflorum Ramat.) belongs to genus Chrysanthemum in the Asteraceae (Compositae) family and is a top leading ornamental plant worldwide. During the last two decades, advances in biotechnology (genetic engineering) and nucleotide sequencing techniques have enabled a deep understanding of biological processes of many ornamental plants at a molecular level. The blue-colored chrysanthemum has been generated and commercialized, and various DNA markers were developed using advanced techniques. Ornamental chrysanthemums are hexaploidy (2n = 2x = 54) and self-incompatible, making it challenging to generate a pure line as breeding resources and a model platform for chrysanthemum research. However, recently, self-compatible pure lines of chrysanthemum (C. seticuspe) were developed, and whole-genome sequencing datasets have been open to the public. Other datasets of whole-genome sequences and transcriptome have been elucidated from wild chrysanthemums, C. nankingense and C. morifolium. In this review, recent progress in the study of ornamental chrysanthemums and the potentiality and importance of wild chrysanthemums as bioresources for ornamental chrysanthemum studies and breeding resources is discussed briefly. Many important resources for studying chrysanthemums are now available and easily accessible. Comprehensive studies with important resources will shed light on chrysanthemum research and breeding of ornamental chrysanthemums at a molecular level. Additionally, this review provides comprehensive information and should pave the way for successful research on ornamental chrysanthemums.

Background: Back pain is associated with a high risk of recurrence. Various physical therapy techniques for back pain have been studied, including reprogramming the central nervous system by integrating sensation and motion with sensory exercise training. Objectives: To aimed verify the effectiveness of sensorimotor training in improving postural stability and pain levels. Design: A randomized controlled trial. Methods: The study population was randomized into a sensory exercise training group and trunk stabilization training group and treated three times a week for 4 weeks. Each group took part in sensorimotor training for 15 minutes or lumbar stabilization exercise for 15 minutes. Results: After the intervention both groups showed Improvements in the variables. There was a significant difference in the dynamic postural stability, limit of stability, and modified visual analog scale scores in the sensorimotor training group compared to the lumbar stabilization exercise group (P<.05). Conclusion: Sensorimotor training appears to be an effective physical therapy exercise program that can be applied in patients with low back pain to improve muscle control ability.

Background: Although the scapular posterior tilt movement could facilitate the lower trapezius (LT) muscle activity, no study identified the effects of the scapular posterior tilt movement on the selective activation of the LT muscle during prone shoulder extension. Objectives: To examine the influences of additional scapular posterior tilt on electromyography (EMG) of the upper trapezius (UT) and the LT muscles during prone shoulder extension. Design: Cross-sectional study. Methods: There were 15 asymptomatic male participants in this study who performed prone shoulder extension with and without scapular posterior tilt movements. For the scapular posterior tilt movements, participants performed visual biofeedback training for scapular movement using motion sensor. During the exercises, the EMG activity of the UT and LT was recorded using surface EMG system. Results: The EMG activity of the LT significantly increased during prone shoulder extension with scapular posterior tilt compared to that of general prone shoulder extension, whereas that of the UT was not significantly different between the two exercises. Moreover, scapular posterior tilt application significantly decreased UT/LT muscle activity ratio. Conclusion: Scapular posterior tilt movement may be emphasized during exercise when facilitating LT muscle activation.

본 연구의 목적은 장애아동 양육자의 물리적·정서적 웰니스 사이에서 지적 웰니스의 매개 효과를 조사하여 효율적인 관리 방안 제시에 필요한 기초자료를 제공하기 위함이다. 연구 대상자는 장애 아동 양육자 177명이며, 자료수집은 2019년 06월부터 2019년 11월까지 하였다. 자료분석은 PROCESS macro를 이용하였다. 일반적인 특성과 변수간 분석은 Independent t-test 및 ANOVA를 통해 통계적으로 유의한 차이가 있는 변수에 대하여는 Scheffé 사후검증을 실시하였다. 변수간 관련성 분석은 Pearson 상관관계 및 위계 다중회귀분석을 하였다. 연구결과, 장애아동 주양육자의 물리적 웰니스와 지적 웰니스는 장애아동 양육자의 정서적 웰니스에 직접적인 영향을 미치며, 또한 물리적 웰니스와 정서적 웰니스 사이에서 지적 웰니스가 매개효과를 제공하고 있다. 이는 장애아동이 가진 물리적 환경의 영향도 중요하 지만, 주양육자에게 적절하고 충분한 지적 교육이 동반되어야 정서적 웰니스 증진이 이루어질 수 있다는 것을 발견하였다. 따라서 다양한 교육 프로그램의 확대는 장애아동 양육자의 웰니스 증진에 기여할 수 있을 것이다.

Universities have recently introduced problem-based learning (PBL) to various subjects to enhance problem-solving skills (including self-directed learning and small-group learning) required in industry. The PBL module was applied to the personal production process in a draping class. A study was based on a questionnaire after conducting two PBL modules with a group of students. Each PBL module included ‘design analysis’, ‘presentation of flat sketch and draping plan’, ‘discussion of the plan’, ‘evaluation of the draping result and correcting the problem’, and ‘final evaluation of the completed project’. Results showed that satisfaction with the PBL method and its activities was higher than satisfaction with existing teaching methods. In particular, among the various components, the ‘design analysis’ and ‘the presentation step of flat sketch and draping plan’ stages were more helpful to students compared to small-group discussion. Moreover, the effects of PBL were observed through student reflection essays, in which students suggested that PBL was very effective in enhancing problem-solving through self-directed and small-group learning. Despite the overall satisfaction with PBL, students expressed some minor difficulties associated with awkwardness with a novel learning method, lack of diverse perspectives among each group, and poor communication skills. Therefore, the study shows that PBL is highly likely to be useful to students when they are solving pattern drafting problems and making samples through self-directed learning and small-group learning.

토종닭의 중요성은 대부분의 농업인 단체에서 자원이 부족한 농업인들을 위한 중요한 사업으로 인식되면서 수년에 걸쳐 증가하고 있다. 그러나 이러한 사업은 낮은 수익률과 비경제적인 문제에 직면 해 있다. 케냐 농축산 연구기구(KALRO) 의 이전 기구이었던 케냐 케냐농업연구기구(KARI)는 토종 닭의 생산성 향상을위한 연구에 앞장서 왔다. KARI의 개량 닭 사양관리 기술은 생산성 저하 문제를 줄이기 위한 연구를 수행 하였다. KALRO는 KOPIA 프로젝트를 통해 지난 5년 동안 Machakos와 Kiambu County의 두 모델 마을에서 기술 이전을 추진 하였다. 본 연구는 KARI의 개량 닭이 식량과 가구 소득에 미치는 영향을 평가하기 위해 수행되었다. 두 카운티 모두 입식률이 2008년 2%에서 증가했으며, 2016년 Machakos County의 시범마을 참여 농업인의 59%와 Kiambu 에서 39%가 입식 한 최고치를 기록하였다. 그러나 병아리 입식은 2017년에 감소하다 다시 2018년에 증가하기 시작하였다. 두 카운티에서 2018 년에 판매 된 계란의 수는 Kiambu카운티에 서 230% 증가하였고, Machakos카운티 시범마을에서는 한 달에 가장 높은 963% 증가를 기록하면서 크게 증가하였다. 이는 주로 연간 180~250개의 알을 낳는 KALRO 개량 닭의 도입에 기인하였다. 이것은 계란 생산과 판매로 식량 및 가구 소득 증가로 이어졌습니다. 이 결과는 KARI 개량 닭이 가정의 식량과 소득 안정에 긍정적인 영향을 미쳤음을 보여주었다.

The intracluster medium (ICM) is expected to experience on average about three passages of weak shocks with low sonic Mach numbers, M . 3, during the formation of galaxy clusters. Both protons and electrons could be accelerated to become high energy cosmic rays (CRs) at such ICM shocks via diusive shock acceleration (DSA). We examine the eects of DSA by multiple shocks on the spectrum of accelerated CRs by including in situ injection/acceleration at each shock, followed by repeated re- acceleration at successive shocks in the test-particle regime. For simplicity, the accelerated particles are assumed to undergo adiabatic decompression without energy loss and escape from the system, before they encounter subsequent shocks. We show that in general the CR spectrum is attened by multiple shock passages, compared to a single episode of DSA, and that the acceleration eciency increases with successive shock passages. However, the decompression due to the expansion of shocks into the cluster outskirts may reduce the amplication and attening of the CR spectrum by multiple shock passages. The nal CR spectrum behind the last shock is determined by the accumulated eects of repeated re-acceleration by all previous shocks, but it is relatively insensitive to the ordering of the shock Mach numbers. Thus multiple passages of shocks may cause the slope of the CR spectrum to deviate from the canonical DSA power-law slope of the current shock.

확산강조 영상은 자기공명영상에서 진단에 매우 중요한 역할을 차지하고 있다. 또한, 높은 b-value를 요구하는 검사가 늘어나는 추세로 확산강조 영상을 얻기 위하여 검사 시간이 길어지게 되며 인공물과 전체적인 신호 감소가 동반하게 된다. 본 연구에서는 확산강조 영상에서 확산 방법을 3스캔 추적 확산강조 영상(3-scan trace diffusion weighted image, tDWI) 에서 DTI의 원리를 적용한 대각선 확산강조 영상(diagonal diffusion weighted image, dDWI)로 변경하여 획득 하였을 때, 두 방법 간의 검사 시간의 단축과 영상을 비교 평가하였다. 농도가 0에서 50%까지 10% 간격인 팬텀을 제작하여 제한된 확산(restrict diffusion)을 단발 스핀에코평면영상(single shot spin echo echo planar, SE-EPI) 기법을 사용하여 검사하였다. 확산 방법을 변경하며 tDWI와 dDWI 검사를 진행하였으며, 획득한 영상을 Image J 프로그램을 이용 하여 농도별 동일한 관심 체적을 설정하여 신호를 측정 후 분석방법은 독립 T-test를 이용하고, 통계적 분석은 SAS를 통해 신호 대 잡음 비와 대조도 대 잡음 비를 비교·분석하였다. p-value 0.05를 기준으로 유의성을 검증하였다. b0를 제외한 b100 이상은 전반적으로 신호 대 잡음 비, 대조도 대 잡음 비가 크며, p-value 0.05 이상으로 나타났다. 확산 방법 간 유의하지 않지만, 전반적으로 dDWI이 tDWI에 비해 평균이 높았다. dDWI 방법이 tDWI에 비해 화질의 변화 없이 검사 시간이 18.35% 단축되는 효과가 있으며, 신호 대 잡음 비와 대조도 대 잡음 비가 소폭 향상된 것으로 나타나 다른 부위의 확산강조 영상을 사용하는 검사에 적용 시 유용할 것으로 사료 된다.

This study investigated the relative predictive power of vocabulary depth and reading fluency on the reading comprehension of advanced Korean EFL learners in college. By doing so, the scope of paradigmatic relations, as part of vocabulary depth, was extended to encompass associative vocabulary as well as synonyms and antonyms, and reading fluency at the discourse level was considered. For this study, 139 college students were tested on a range of vocabulary depth tests, as well as reading comprehension and text-level reading fluency. The findings revealed that although both vocabulary depth and reading fluency are significant contributors to reading comprehension abilities, the predictability of vocabulary depth was larger than that of reading fluency. In addition, associative vocabulary not only revealed additional predictive power for reading comprehension on top of reading fluency, synonyms and antonyms, but also showed stronger predictability compared to synonyms and antonyms. These results highlight that both vocabulary depth, especially the knowledge of how words are related together, and text-level reading fluency play a crucial role in boosting the reading comprehension abilities of even advanced L2 readers.