There are about 15,000 kinds of mushrooms found worldwide and 2,000 kinds of edible mushrooms. There are about 1,500 kinds of mushrooms native to Korea, among which about 300 kinds of edible mushrooms are counted. In Korea, mainly Lentinus edodes (shiitake mushroom), Tricholoma matsutake (pine mushroom), and Sarcodon aspratus (Neungi mushroom) are edible, and they are imported from China due to insufficient production. The purpose of this study was to compare the nutritional quality of Chinese and Korean frozen Tricholoma matsutake and Sarcodon aspratus. The crude fat content of frozen Tricholoma matsutake was 1.1 ~ 1.5% in Chinese and 0.5% in Korea. Korea products were lower than Chinese ones. The content of crude protein in Chinese was 16.4 ~ 20.9% and that of Korea was 21.2%. In crude ash content, there are some differences between regions. The crude protein content of frozen Sarcodon aspratus was 18.9% in Chinese and 19.4% in Korea. The crude fat content was 1.2% in Chinese and 0.9% in Korea. There was no significant difference in the contents of crude ash.

The world mushroom market is continuously expanding due to the improvement of production technology and the continuous increase of demand. Production and consumption of mushrooms are increasing in Korea and imports are increasing year by year. Most of the imported mushrooms are from China. The Lentinus edodes (shiitake mushroom) are in dry form, but Tricholoma matsutake (pine mushroom) and Sarcodon aspratus (Neungi mushroom) are imported in frozen form. It is suspected that frozen Tricholoma matsutake and Sarcodon aspratus are coated with water to increase the weight excessively. In this study, we compared the moisture content and drip% of Chinese and Korean frozen Tricholoma matsutake and Sarcodon aspratus to determine the presence or absence of water coating. The moisture content of frozen Tricholoma matsutake was highest in Yunnan (China) at 94.7%, followed by Tibetan (China) at 92.5%, Yanji (China) at 90.6% and Korea at 88.5%. The moisture content of frozen Sarcodon aspratus was 92.4% in China and 91.6% in Korea. The drip% of frozen Tricholoma matsutake was 23.1% in Tibetan (China), 22.1% in Yunnan (China), 14.5% in Yanji (China) and 11.7% in Korea. The amount of drip% of Korea and Chinese frozen Sarcodon aspratus was 20.8% and 22.7%, respectively. Moisture content and drip% of frozen Sarcodon aspratus were similar to those of Chinese and Korea. Frozen Tricholoma matsutake imported from China showed high moisture content and drip% than Korea ones. These results indicated that water coating is possible in frozen Tricholoma matsutake imported from China.

Honey production from approximately 1.7 million colonies owned by around 21 thousand beekeepers was almost 36 thousand M/T in Korea. Pollen has used as a food and medicine from before the Joseon Dynasty period in Korea. Pollen grains such as acorn (Quercus acutissima), actinidia (Actinidia arguta) and green tea (Camellia sinensis) are popular in the markets in Korea. But stiff pollen wall hindered dissolution of polysaccharides and lowered extraction efficiency. In the present study, we measured the antioxidant activity and the total polyphenol content from the pulverized and lyophilized green tea pollen grains inoculated with 6 kinds of fungi to confirm the husk removal effect. The total polyphenol content of green tea pollens was highest in lyophilized pollen medium inoculated with Armillaria mellea, and was lowest in pollen inoculated with Lentinula edodes. Total polyphenol content of the lyophilized pollen was higher than that of the refined pollen and the pulverized pollen in green tea pollen germinated with A. mellea. The total polyphenol content of the lyophilized green tea pollens germinated with A. mellea was 1.4-fold higher than that extracted with water. Measurement of antioxidant activity using the DPPH free radical scavenging method showed that the lyophilized green tea pollen grains germinated with A. mellea had the highest and that germinated with L. edodes was lowest in antioxidant activities. The lyophilized green tea pollen grains germinated with A. mellea was 2 to 4 times higher than that extracted with water in the antioxidant activity of DPPH free radical scavenging. Many germinated cells were formed around pore of green tea pollen inoculated with L. edodes, while those were formed at the end of hyphae derived from green tea pollen grains inoculated with A. mellea.

A. auricular-judae cultivars, ‘Yong-a’ was cultivated in JARES on 2015. Ear mushroom have been consumed the form of dried mushrooms imported from china, but the production and consumption of domestic ear mushroom have been increasing recently. However, how the different drying methods affect the quality of domestic varieties is not clear. Therefore, we examined the effects of drying methods to improve the competitiveness of dried ear mushrooms using domestic cultivar, ‘Yong-a’. Ear mushroom samples were dried using sun-drying and hot air drying(50, 60°C) and combined of two methods(hot air drying at 50, 60°C after 3,6 and 9 hours sun drying) and drying time, rehydration ratio, color and hardness, vitamin D2 contents were evaluated. Drying time was the fastest at 60°C hot air drying(11.5h) and the slowest at 50°C hot air drying(16h) and drying time was shorter as the sun drying time was longer. The rehydration ratio was the highest at 60°C hot air drying(18.6%) and increased as the drying time decreased. After drying and rehydration, the color showed no significant difference between drying methods. The hardness of rehydrated mushroom was highest at sun drying and 60°C hot air drying. The contents of ergosterol were not significantly different by drying methods. However, vitamin D2 was lowest at 50 °C and 60°C hot air drying (4.22mg%, 3.87mg%) and highest at sun drying and 50°C hot air drying after 6 hours sun drying (12.52mg%, 12.99mg%). vitamin D2 contents was higher at 50°C hot air drying than 60°C. According to the results of color, hardness, vitamin D2 contents of dried ear mushroom, sun drying is the best method. When the sun drying is not possible, hot air drying at 60°C is suitable, but it is better to use sun drying method to increase the vitamin D2 contents.

The purpose of this study was to investigate the effects of lumbar stabilization and sling exercise on visual analogue scale (VAS), Korean Oswestry Disability Index (KODI), and stability index (ST) in patients with chronic lower back pain (CLBP). This study included participants with a VAS scale of 6 or higher and a KOD of 20-40%. They were randomly divided into a mat group (n=15) to perform lumbar stabilization exercises and a sling group (n=15) to perform sling exercises, and then they underwent a four-week experiment. The experimental results of this study were as follows: the VAS and KODI showed a statistically significant difference (P<.05) and a larger effect size within each group after the intervention, it exhibited no statistically significant difference between the two groups (P>.05). There were significant differences in ST before and after intervention in each group (P<.05), however there was no significant difference between the groups. The present study suggest that the effects of lumbar stabilization exercise and sling exercise are similar.

In the present study, domain evolution processes of a near-morphotropic PZT ceramic during poling was studied using vertical piezoresponse force microscopy (PFM). To perform macroscopic poling in bulk polycrystalline PZT, poling was carried out in a stepwise fashion, and PFM scan was performed after unloading the electric field. To identify the crystallographic orientation and planes for the observed non-180o domain walls in the PFM images, compatibility theory and electron backscatter diffraction (EBSD) were used in conjunction with PFM. Accurate registration between PFM and the EBSD image quality map was carried out by mapping several grains on the sample surface. A herringbone-like domain pattern consisting of two sets of lamellae was observed; this structure evolved into a single set of lamellae during the stepwise poling process. The mechanism underlying the observed domain evolution process was interpreted as showing that the growth of lamellae is determined by the potential energy associated with polarization and an externally applied electric field.

목 적 : 본 연구에서는 피로와 스트레스가 안구건조에 미치는 영향을 알아보고자 하였다. 방 법 : 만 19세 이상 성인 365명을 대상으로 설문 자료를 이용하여 대상자의 인구사회학적 변인, 피로심 각도 척도, 스트레스 척도, 안구건조 척도를 자기 기입식으로 하였으며, 단면연구를 시행하였다. 결 과 : 안구건조가 여성에서 유의한 차이로 높게 나타났으며, 음주를 많이 할수록, 만성 질환이 있는 사 람에게서 안구건조가 높게 나타났다. 40세 이상, 흡연자, 수술한 눈에서 안구건조가 조금 높게 나타났으나 유의한 차이를 보이지 않았다. 반면에, 렌즈 착용한 그룹에서 안구건조가 유의한 차이로 높게 나타났다. 고 피로 그룹일수록 안구건조정도가 높았으며, 스트레스 지수가 증가할수록 안구건조도 증가하는 것으로 나타 났다. 피로가 높은 군에서 안구건조 위험도가 1.53배 높았으며, 콘택트렌즈 착용하는 군에서는 안구건조 위 험도가 3.87배 높게 나타났다. 결 론 : 피로와 스트레스가 높을수록 안구건조를 많이 느끼는 것을 확인하였다.

The continuous exploration in deep seawater from Korea makes us lead the discovery of ancient Chlorophyta, Palmophyllum, in the Korean coast. The phylogenetic analyses of 18S rRNA and rbcL genes demonstrate that our specimens are Palmophyllum crassum (Naccari) Rabenhorst, recorded in Japan and clearly distinguished from P. umbracola from New Zealand and California, USA. Palmophyllum crassum grows in the subtidal region, 8-30 m deep, and has a crustose thallus which is closely adherent to substrates such as non-geniculate crustose coralline algae, sponge, shells, or rocks. P. crassum is composed of numerous spherical cells embedded in the gelatinous matrix. The discovery of this ancient green seaweed implies that the Korean coast is one of the hotspots of algal species diversity and has the suitable marine environment for algal speciation. We suggest the grounds to conserve the Korean coast environmentally as the biodiversity center of marine species by studying the phylogeny of seaweeds.

Freshwater algae (green algae, blue-green algae and flagellated algae) were collected at 103 freshwater sites (including lakes, ponds, swamps, streams, and rivers) throughout South Korea March 2015-October 2016, and were identified using light microscopy. A total of 345 taxa in 2015 and 329 taxa in 2016 were identified and among them, 6 taxa in 2015 and 8 taxa in 2016 were new recorded species in Korea; The new recorded species were Characiopsis malleolus in Xanthophyceae, Phacus mammillatus in Euglenophyceae, Epipyxis utriculus and Lagynion ampullaceum in Chrysophyceae, Bicosoeca planctonica in Bicosoecophyceae and Salpingoeca frequentissima in Choanoflagellatea in 2015, and were Ankistrodesmus bernardii and Quadrigula korsikovii in Chlorophyceae, Didymocystis planctonica in Treubouxiophyceae, Spirulina nodosa, Raphidiopsis curvata and Geitlerinema claricentrosum in Cyanophyceae and Lagynion macrotrachelum in Chrysophyceae, Bicosoeca oculata in Bicosoecophyceae and Salpingeoca rosetta in Choanoflagellatea in 2016.

목 적 : 충청남도 지역에서 근무하는 안경사를 대상으로 보수교육 실태현황을 조사, 분석하여 보수교육의 질 향상에 필요한 기초자료로 제공하고자 하였다. 방법 : 2017년도 충청남도 지부 보수교육에 참여한 403명 중 353명이 설문조사에 응답하였다. 응답 내용이 불성실한 설문지를 제외한 330개를 분석 대상으로 하였다. 결 과 : 남자 273명, 여자 57명을 대상으로 하였으며, 평균연령은 43세이었다. 안경원 개설자는 189명, 종사자는 141명이었다. 현재 재직 중인 안경원에서 근무를 지속할 의지가 있는 사람이 210명으로 가장 많았 다. 보수교육 경험 차이는 성별, 나이, 고용 형태, 결혼, 근무 경력, 소득에서 유의하게 나타났다. 안경사들 은 대한안경사협회로부터 보수교육에 관한 정보를 받고 많이 받고 있으며, 법적 제재를 피하기 위해 보수교 육에 참여하는 경우가 많았다. 보수교육으로 최신 기술 및 제품 정보가 가장 많이 필요하다고 응답하였다. 결 론 : 체계적인 보수교육 관리를 통해 장기 미이수자가 발생하는 것을 방지할 수 있으며, 안보건 의료 전문 인력을 확보할 수 있다. 추후 보수교육 이수자의 만족도와 요구도를 지속적으로 추적하는 연구가 필요 하며, 보수교육 프로그램을 개선 및 개발하려는 노력이 필요할 것으로 사료된다.

An investigation focusing on the unrecorded and taxonomically undescribed indigenous has been done since 2006. Samples were collected from various sites in the coastal and offshore waters of Korea as well as around Jeju Island. Since 2008, 16 unrecorded species belonging to the family Gymnodiniaceae have been found. The species were as follows: Amphidinium thermaeum (2015), Cochlodinium convolutum (2015), C. strangulatum (2015), Gymnodinium abbreviatum (valid name: G. gracile), G. arenicola (2015), G. gracile (2015), G. dorsalisulcum (2015), G. microreticulatum (2014), G. micrum (2016) (valid name: Karlodinium micrum), G. pyrenoidosum (2016), G. simplex (2015), G. veneficum (2016) (valid name: Karlodinium veneficum), Gyrodinium aureum (2015), G. fusiforme (2015), G. dominans (2014), and Nusuttodinium latum (2016) (valid name: Amphidinium latum). (The numbers in parentheses refer to the year that the species was found). These species were newly recorded in Korean waters in this study.

The order Prorocentales currently includes two genera Prorocentrum Ehrenberg and Mesoporos Lillick. The Prorocentrum genus is a predominant group throughout the year found in Korean waters. To date, the Prorocentrum genus includes 31 species and the Mesoporos genus has only one species in Korean waters. In this study, we identified one Mesoporos species and three Prorocentrum species around a coast of Jeju Island, and described them as newly recorded species in Korean waters.

This study was designed to optimize ethanol extraction process of unfertilized corn silk (UCS) to maximize phytochemical contents and bioactivities. The response surface methodology (RSM) with central composite design (CCD) was employed to obtain the optimal extraction conditions. The influence of ethanol concentration, extraction temperature and extraction time on total polyphenol contents, total flavonoid contents, maysin contents, 2,2-diphenyl-1- picrylhydrazyl(DPPH) radical scavenging activities and tyrosinase inhibition were analyzed. For all dependable variables, the most significant factor was ethanol concentration followed by extraction temperature and extraction time. The following optimum conditions were determined by simultaneous optimization of several responses with the Derringer’s desirability function using the numerical optimization function of the Design-Expert program: ethanol concentration 80.45%, extraction temperature 53.49°C, and extraction time 4.95 h. Under these conditions, the predicted values of total polyphenol contents, total flavonoid contents, maysin contents, DPPH radical scavenging activity and tyrosinase inhibition were 2758.74 μg GAE/g dried sample, 1520.81 μg QUE/g dried sample, 810.26 mg/100g dried sample, 56.86% and 43.49%, respectively, and the overall desirability (D) was 0.74.

Humic acid (HA) is known to consist of various kinds of polymeric organics, their detailed structures can vary depend on sample sources such as organic manure, composts, peat, and lignite brown coal, and largely exists in grassland soils. HA possesses diverse positive effects that not only increase plant growth but also improve soil fertility. Recently, we have manufactured a co-polymeric product of catechol and vanillic acid (CAVA) synthesized artificially, and found that CAVA as a HA mimic increases seed germination and salt tolerance in Arabidopsis. In this study, we examined whether HA or CAVA affects to seedling growth in alfalfa. Foliar application of HA or CAVA increased alfalfa seedling growth including aerial and in root parts. HA or CAVA dramatically enhanced size of leaf and root, whereas HA significantly displayed higher bioactivity than CAVA. Taken together, CAVA acts like as a HA mimic in alfalfa that could apply as an alternation supplement to enhance plant growth and productivity.

목적 : 이 설문 연구는 안경사법에 대한 일반소비자 집단의 인식도를 조사하고, 그로부터 안경사법을 입 법하기 위한 효과적인 입법 전략을 도출하는 것을 목적으로 하였다. 방법 : 2016년 5월부터 8월까지 서울 지역에서 일반인 200 명을 상대로 무작위 1대1 대면 설문조사를 실 시하고 그 결과를 시력교정 경험자와 미경험자 집단으로 구분하여 분석하였다. 결과 : 일반 소비자 집단의 안경원법에 대한 인식도는 매우 낮은 편이었으며, 그로 인하여 안경사법에 대 한 찬반 의견이나 안경사법의 통과 가능성을 묻는 설문에도 ‘모르겠다’는 중립적 답변이 매우 많았다. 안경 사법이 통과되기 어려운 이유와 그에 대한 해결 방법을 묻는 설문에서는, ‘안과의사들의 반대’와 ‘국민적 합 의 미흡’을 이유로 답하고 해결 방법도 ‘안과의사들과의 합의’와 ‘국민적 합의 제고’라고 응답한 비율이 높았 다. 안경사의 타각적 굴절검사가 허용되면 수용하겠다는 긍정적인 답변이 60%를 넘었으며, 안경사의 타각 적 굴절검사는 의료행위가 아닌 광학적 측정이라는 답변 또한 높은 응답률을 기록하였다. 결론 : 안경사법의 입법 과정에서 일반 소비자들의 낮은 인식도가 큰 걸림돌로 작용하고 있음을 알 수 있 었다. 안경사법의 입법을 위해서는 일반인을 대상으로 하는 홍보를 강화하고, 안과의사 집단과의 합의에 주 력하는 것이 가장 효과적인 방법임을 설문 결과로부터 유추할 수 있다. 일반 소비자 집단은 타각적 굴절검사 가 의료행위라는 인식이 약하고 안경사의 타각적 굴절검사 수용에 적극적이므로, 안경사법의 내용에 대한 대국민 홍보가 강화된다면 안경사법의 입법 과정에 크게 기여할 수 있으리라 예상된다.

목적 : 본 연구는 안경사의 감정노동 수준을 파악하여 감정노동에 대한 대책마련을 위한 기초자료를 제공 하고자 한다. 방법 : 인천지역 안경사가 응답한 80부의 설문지를 분석하였다. 감정노동의 측정도구는 Morris와 Feldman이 개발하고 김민주가 번역한 것을 안경사에 맞게 수정하였다. 결과 : 안경사의 감정노동 수준은 평균 3.24±0.55점이었다. 이는 같은 보건직종인 간호사(3.37±0.56) 및 치과위생사(3.28±0.56)와 비슷한 수준이었다. 그러나 생활지도원(2.72±0.55)과 버스기사(3.03±0.78) 보다 상대적으로 높았으며 콜센터직원(3.42±0.62)과 호텔종사자(3.60±0.99)보다 상대적으로 낮았다. 감정 노동의 하위항목인 감정표현의 빈도, 감정표현 규범의 주의성, 감정의 부조화 점수는 각각 3.64±0.63점, 3.11±0.59점, 2.98±0.70점이었다. 대상자의 주 당 평균 근로시간은 65.6±9.6시간이었고, 일일 근로시간 이 10시간 이하 및 10시간 초과인 경우 각각 2.94±0.42점과 3.34±0.55점이었다(p=0.005). 또한 주간 근 무일수가 5일 이하(2.99±0.54)와 5일 초과(3.31±0.53) 사이에는 유의한 차이가 있었다(p=0.032). 결론 : 안경사의 감정노동은 하루 근무시간이 10시간 이상일 때 더 높은 수준으로 나타났다. 따라서 감정 노동에 영향을 끼치는 요인을 찾아 근로환경을 개선하고 감정노동을 줄이기 위한 추가적인 연구가 필요하다.

This study was conducted to examine the effect of corn (Zea mays L.) - soybean (Glycine max L.) silage prepared by intercropping method on the nutritive value of the silage, in vitro rumen fermentation characteristics, dry matter degradability, as well as milk yield and milk composition of dairy cows. In a couple of experiments intercropped corn-soybean silage (CSBS) was compared with corn silage (CS) and/or Italian ryegrass hay (IRG). Numerically, CSBS had higher crude protein, ether extract, and lactic acid contents compared to CS. In vitro rumen fermentation analysis demonstrated that up to a 24-h incubation period, both CS and CSBS showed higher total gas production, ammonia N concentration, and dry matter degradability compared to IRG (p<0.05). The investigation on animals was conducted in a commercial dairy farm located in Gyeongju, South Korea, employing 42 Holstein cows that were divided into 2 group treatments: CS and CSBS in a completely randomized design. Although no significant difference was observed in milk yield, animals fed on CSBS showed significantly higher milk protein (p<0.05) and milk fat content (p<0.01), compared to animals fed on CS. Taken together, our findings indicate that corn-soybean silage that is cultivated, harvested, and prepared through intercropping can improve the protein content of the silage, and can also enhance in vitro rumen fermentation, dry matter degradability, and performance of dairy cattle.

Resveratrol was incorporated into various combinations of single- and double-layer nanoemulsions, prepared by selfassembly emulsification and complex coacervation with chitosan, alginate, and β-cyclodextrin, respectively. Resveratrol nanoemulsions were composed of medium-chain trigacylglycerols (MCTs), Tween ® 80, water, chitosan, alginate, and β-cyclodextrin. The corresponding mixtures were formulated for the purpose of being used as a nutraceutical delivery system. Resveratrol nanoemulsions were obtained with particle sizes of 10-800 nm, with the size variation dependent on the emulsification parameters including the ratio of aqueous phase and surfactant ratio. Resveratrol nanoemulsions were characterized by evaluating particle size, zeta-potential value, stability, and release rate. There were no significant changes in particle size and zeta-potential value of resveratrol nanoemulsions during storage for 28 days at 25°C. The stability of resveratrol in the double-layer nanoemulsions complexed with chitosan or β-cyclodextrin was higher, compared with the single-layer nanoemulsions.

Hay-making is one of the most common forage preservation practices in livestock operations. The objective of hay-making is to minimize nutrient loss by shortening field drying time. Measuring the impacts of cutting height of forage crop is necessary to optimize hay production balancing yield and quality, in order to obtain substantial biomass increase through harvest of regrowth. This experiment was conducted to investigate the impact of cutting height of rye (Secale cereale L.) on drying rate and hay quality. Heading stage rye was harvested at 8cm or 15cm stubble heights. Hay was daily tedded at 09:00 and sampled at 09:00, 13:00 and 17:00 to determine moisture content (MC). After two month of preservation, CP (crude protein), ADF (acid detergent fiber), NDF (neutral detergent fiber), IVDMD (in vitro dry matter disappearance), TDN (total digestible nutrient), RFV (relative feed value), DM (dry matter) loss, visual scores and total fungi count were determined for estimation of hay quality. Cutting height at 15cm could enhance the drying rate and CP content (p<0.05), but also increases DM loss (p<0.05) compared to cutting at 8cm. Cutting heights did not affect ADF, NDF, IVDMD, TDN and RFV value (p>0.05). Visual scores of rye hays cutting at 8cm and 15cm, ranged from 83 to 85. Cutting at 8cm tended to maintain higher core bale temperature and fungal count than cutting at 15cm during preservation, but there was no significant difference.

In order to compare greenhouse gases emission from different animal manures and to explore how different animal manures effect on soil mineralization, three kinds of materials, cattle, goat and chicken manure were amended to soil for 14 days incubation as CtS (cattle manure-amended soil), GS (goat manure-amended soil) and ChS (chicken manure-amended soil). Cumulative NH3 emissions in all treatments were rapidly increased until day 7 and then it was slightly increased in three manure-amended soils but maintained in control until day 14. GS had the highest NH3 emission at 0.14 mg kg-1 during the entire experimental period. Emissions of CO2 were highly increased by 7.8-, 9.0- and 12.4-fold in CtS, GS and ChS, respectively, compared to control at day 14. A significant increase of N2O emission in all treatments occurred within 5 days and then it was slightly increased until day 14. N2O emission was 2-fold higher in all manure-amended soils than that of control. Compared to day 1, inorganic N (NH4 + plus NO3 --N) content was highly increased in all four treatments at day 14. The increase rate was the highest in CtS treatment. Net N mineralization was increased by 4.0-, 2.4- and 2.9-fold in CtS, GS and ChS, respectively, compared to control. These results indicate that increase of NH3, CO2 and N2O gas emissions was positively related to high N mineralization.