We analyzed the hair microstructure of Korean small terrestrial mammals, such as Rodentia and Lagomorpha, to classify the characteristics of hair morphology. Micromys minutus showed a unicellular irregular type of medulla structures; on the other hand, the other mammals showed multicellular structures. Regarding the cuticular scale structures, the Rodentia species exhibited a narrow and broad diamond petal type, while the Lagomorpha species exhibited an elongated petal type. Interestingly, the hair cross-sections showed quite unique characteristics. We constructed hair identification keys to distinguish species with a single hair. The dichotomous key of Rodentia and Lagomorpha can be used for their behavioral ecology and dietary analysis of upper predators to serve as the basis for ecological research.

Busulfan is the most commonly used drug for preconditioning during the transplantation of hematopoietic stem cells and male germ cells. Here, we describe side effects of high doses of busulfan in male mongrel dogs. Busulfan was intravenously administered to three groups of dogs at doses of 10, 15, and 17.5 mg/kg body weight. The total white blood cell, neutrophil, eosinophil, lymphocyte, monocyte, and platelet counts steadily reduced in a dose-dependent manner following busulfan treatment. The white blood cell, neutrophil, and monocyte counts recovered after 6 weeks of busulfan treatment, however, the eosinophil, lymphocyte, and platelet counts remained unaltered. Additionally, there was one fatality in the each of the groups that were administered 15 and 17.5 mg/kg busulfan. The gross lesions included severe hemorrhage in the stomach, intestinal tracts, mesentery and urinary bladder. Microscopic investigation revealed severe pulmonary edema and hemorrhage in the lungs, and severe multifocal to coalescing transmural hemorrhage in the intestines and urinary bladder. These results indicated that treatment with busulfan at doses higher than 15 mg/kg initiates severe bleeding in the internal organs and can have fatal results.

Recent progress has been made to establish intestinal organoids for an in vitro model as a potential alternative to an in vivo system in animals. We previously reported a reliable method for the isolation of intestinal crypts from the small intestine and robust three-dimensional (3D) expansion of intestinal organoids (basal-out) in adult bovines. The present study aimed to establish next-generation intestinal organoids for practical applications in disease modeling-based host-pathogen interactions and feed efficiency measurements. In this study, we developed a rapid and convenient method for the efficient generation of intestinal organoids through the modulation of the Wnt signaling pathway and continuous apical-out intestinal organoids. Remarkably, the intestinal epithelium only takes 3-4 days to undergo CHIR (1 µM) treatment as a Wnt activator, which is much shorter than that required for spontaneous differentiation (7 days). Subsequently, we successfully established an apical-out bovine intestinal organoid culture system through suspension culture without Matrigel matrix, indicating an apical-out membrane on the surface. Collectively, these results demonstrate the efficient generation and next-generation of bovine intestinal organoids and will facilitate their potential use for various purposes, such as disease modeling, in the field of animal biotechnology.

The present study analyzed small group interaction in the videoconferencing-mediated English class at a university in South Korea. Adopting the ‘affordance’ construct (van Lier, 2004) as a conceptual framework for interpreting small group work, the goals of the research are to 1) identify the types of linguistic affordances emerging in videoconferencing-mediated small group work and 2) examine learners’ perceptions and responses to the linguistic affordances. Data were collected from two major sources: 40 video recording files of small group observation and post-class interviews with four students. The paper analyzed 13 extracts selected from the transcription of the video recordings. The overall results of the research suggest that a range of linguistic affordances emerged through interaction and participants responded to the affordances they perceived in various ways. Three types of linguistic affordances were observed in the data: technology-generated affordances, learner-generated affordances, and learner-to-learner-generated affordances. The study findings have important implications for providing new insights into the operationalization of the affordance construct as well as advancing the understanding of the affordance perspective of L2 learning.

목적: 국민건강영양조사 제7기(2016-2018) 원시자료를 이용하여 황반변성에 영향을 줄 수 있는 만성질환을 파악 하고자 수행되었다. 방법 : 국민건강영양조사 제7기(2016-2018) 자료 중 40세 이상의 성인 중에 황반변성 진단여부에 응답한 4,356명의 대상으로 만성질환과의 관계를 분석하였으며, p<0.05인 경우 유의한 것으로 판단하였다. 결과 : 황반변성은 연령에 따라 유병률이 높아졌는데, 연령과 관계된 만성질환 중 심근경색증에서만 유의한 결 과를 얻을 수 있었다. 또한, 심근경색증의 위험요인인 이상지질혈증과 이상지질혈증을 일으키는 고콜레스테롤혈증 도 황반변성과 관계가 있는 것으로 나타났다. 결론 : 황반변성은 연령과 관련된 만성질환 중 심근경색증, 이상지질혈증, 고콜레스테롤혈증과 관련이 있음을 확인하였다.

This study investigated changes of milk production in dairy cows intramuscularly injected with drugs containing dexamethasone (DXM). Three types of dexamethasone formulations (Bueunde® (DXM 0.5 mg/mL), Dexason INJ.® (DXM 1 mg/mL) and Dexolone-20 inj.® (DXM 1 mg/mL)) were intramuscularly injected into sixteen healthy dairy cows each. Bueunde® was intramuscularly injected into 8 dairy cows with 5 mg (BED-1) and 10 mg (BED-2) of DXM once a day for 3 consecutive days, respectively. Dexason INJ.® was intramuscularly administered once into dairy cows with 20 mg (DXS-1, n=8) and 40 mg (DXS-2, n=8) of DXM, respectively. Dexolone-20 inj.® was intramuscularly injected once into dairy cows with 20 mg (DXS-1, n=8) and 40 mg (DXS-2, n=8) of DXM, respectively. Milk production (MP) of BED-1 and BED-2 significantly decreased during the drug administration and up to 48-hour post-drug treatment. Compared with the MP before drug administration, the MP of DXS-1 and DXL-1 was meaningfully decreased by 36 and 24-hour post-drug administration, respectively, and that in both DXS-2 and DXL-2 significantly decreased until 48-hour post-drug treatment. In conclusion, it was confirmed that the MP temporarily decreased by 48 hours after administration of DXM to dairy cows.

The ovary undergoes substantial physiological changes along with estrus phase to mediate negative/positive feedback to the upstream reproductive tissues and to play a role in producing a fertilizable oocyte in the developing follicles. However, the disorder of estrus cycle in female can lead to diseases, such as cystic ovary which is directly associated with decline of overall reproductive performance. In gene expression studies of ovaries, quantitative reverse transcription polymerase chain reaction (qPCR) assay has been widely applied. During this assay, although normalization of target genes against reference genes (RGs) has been indispensably conducted, the expression of RGs is also variable in each experimental condition which can result in false conclusion. Because the understanding for stable RG in porcine ovaries was still limited, we attempted to assess the stability of RGs from the pool of ten commonly used RGs (18S, B2M, PPIA, RPL4, SDHA, ACTB, GAPDH, HPRT1, YWHAZ, and TBP) in the porcine ovaries under different estrus phase (follicular and luteal phase) and cystic condition, using stable RG-finding programs (geNorm, Normfinder, and BestKeeper). The significant (p < 0.01) differences in Ct values of RGs in the porcine ovaries under different conditions were identified. In assessing the stability of RGs, three programs comprehensively agreed that TBP and YWHAZ were suitable RGs to study porcine ovaries under different conditions but ACTB and GAPDH were inappropriate RGs in this experimental condition. We hope that these results contribute to plan the experiment design in the field of reproductive physiology in pigs as reference data.

Based on many previous studies, this study reviewed the effects of needle-free intradermal injection (NFI) on the prevention of the incidence of lesions at the injection site in pork from pigs vaccinated with the foot-and-mouth disease vaccine, the reduction of the vaccine dose, the stress-relief of vaccination, and the reduction of farm worker-related injuries. The vaccine and drugs injected with the NFI pass through the tissue and are widely dispersed along the path of least resistance, resulting in a vastly dispersed, spider-web-like distribution of the medication and absorbed quickly. In addition, since NFI is inoculated into the dermal layer where immune cells are abundantly distributed, even a small amount of vaccination shows an antibody-forming effect similar to that of the conventional needle intramuscular injection. NFI is a method suitable for animal welfare because the vaccine or drug is widely dispersed over the inoculation site and causes less pain or stress than the needle intramuscular injection. Use of NFI system for the vaccination and drug delivery improves work efficiency in farms and reduces the incidence of musculoskeletal disorders in farm workers. In the future, it is judged that in-depth studies are needed on the effect of improving productivity and reducing the dose of antibiotics through NFI.

The purpose of this study was to examine the extent to which influencing factors impact the transference willingness of farmers. The research method is based on the survey data on transference willingness, and the binary logistic model was used to analyze the influencing factors. The results show that the educational level, annual household income, farm labor, and farmers' understanding of land transfer policy notably influence land transfer. Based on the results, suggestions include improving farmers' social security system, improving a sound market for rural land transfer, and deepening the promotion and explanation of land-related policies.

This study investigated chlorpheniramine maleate (CPM) residues in milk of intramuscularly dosed dairy cows and established the withdrawal time (WT) of CPM in milk. Sixteen healthy Holstein cows were injected with 10 (CPM-1) and 20 mL (CPM-2) of the drug containing 4 mg/mL of CPM, respectively. After administration of CPM, milk samples were collected from all cows at 12 hour intervals for 5 days. CPM residue concentrations in milk were determined using LC-MS/MS. The correlation coefficient of the calibration curve was 0.9956, and the limits of detection and quantification (LOQ) were 0.6 and 1.0 μg/kg, respectively. Recoveries ranged between 98.5-115.0%, and coefficient of variations were less than 10.96%. After treatment, CPM in CPM-1 and CPM-2 was detected below the LOQ in all milk samples at 12 hours. According to the European Medicines Agency’s guideline on determination of withdrawal periods for milk, withdrawal periods of both CPM-1 and CPM-2 in milk were established to 12 hours. In conclusion, the developed analytical method is sensitive and reliable for detection of CPM in milk, and the estimated WT of CPM in bovine milk will contribute to ensuring the safety of milk.

This study examines the concept of Korean ethnicity by looking into how the Choseonjok, ethnic return migrants from China, are perceived in online news comments. Data were collected from 3,109 comments, which were written in response to 28 news articles regarding the Choseonjok, and analyzed using a Python-based text-mining technique and critical discourse analysis. The findings show that the commenters perceived the Choseonjok negatively, including as potential criminals or social vice. They not only placed the Choseonjok in an inferior position to pure Koreans but also excluded them from the category of compatriots, arguing that speaking the same language and sharing a similar appearance were not enough to make the ethnic return migrants Korean compatriots. This study critically demonstrates how a group of ethnic return migrants is depicted in online public discourses and how this portrayal can shed light on the conceptualization of Korean ethnicity in this era of multiculturalism.

최근 AI 기술은 하루가 다르게 빠르게 발전하고 있고, AI기술은 각 분야에서 다양하게 사용되어지고 있다. 본 논문은 예술분야에서 AI기술의 활용으로 COVID-19 상황에서 인간관계, 개인적인 이유로 지친 마음을 위 로해주는 힐링 게임을 제작하였다. 제작한 힐링게임에서는 주로 Self-help-therapy의 효과를 얻을 수 있어, 치 료자의 도움없이 이용자가 힐링게임을 통하여 일상적 이용과정에서 치유적 효과를 얻을 수 있는 것을 기대 하고 있다. 게임 리뷰 데이터를 통계 분석하여 힐링게임으로 대중들이 요구하는 부분을 수용하여 힐링게임 이 제작되었으며, 사용자는 게임 시작 전 간단한 스토리라인과 AI와 상호작용할 수 있는 간단한 대화를 통 화여 Self-help-therapy 효과를 얻을 수 있었다.

This study aims to investigate the proper mixing treatment concentration of ozone (O3) and sucrose to preserve and extend the vase life of the cut rose flowers ‘Dominica’. The vase solution was prepared using tap water, 3% sucrose, ozone 5.5 mg L-1, and 3% sucrose with ozone 5.5 mg L-1. The vase life was the highest in the tap water and ozone treatments at 16.3 and 16.1 days, respectively. The vase life of ozone with sucrose treatment was 6.9 days, which was 9.4 days lower than that of the control. Compared to a single treatment, the vase life termination symptoms for ozone with sucrose treatment decreased petal wilting and increased bent necks. Relative fresh weight and vase solution uptake increased up to 4 days after treatment and decreased from 2 days before vase life termination. The rate of change in petal color was high in L*, a*, and b* for the sucrose treatment than after harvest, and low for the ozone treatments. The maximum relative flower size increase rates after treatment were 195% in the control, 186% in the sucrose treatment, 171% in the ozone treatment, and 155% in the ozone with sucrose treatment.

Swine atrophic rhinitis is a respiratory disease that causes nasal turbinate loss and septal deformation due to Bordetella bronchiseptica and Pasteurella multocida. Turbinate loss facilitates pathogens to infect lungs, which leads to various respiratory diseases and productivity reduction. In this study, descriptive analysis was implemented for atrophic rhinitis and pneumonia. From 6 pig farms shipped to slaughterhouses in Chungbuk province, 20 heads and 20 lungs were collected by each farm from March 2020 to September 2020. Their atrophic rhinitis lesions and lung lesions were scored and blood samples were also collected to test the seroprevalence of several respiratory diseases. Pasteurella multocida from nasal swab was cultured and antibiotic resistance tests were performed. Correlation between atrophic rhinitis scores and lung lesion scores was not found. Abdominal nasal lesions were more severe than dorsal lesions. Differences in lung lesion scores were relatively small between lobes. The score of pneumonia was higher in castrated pigs than in female pigs. There was no relationship between lesion score and seroprevalence of respiratory diseases. Antibiotic resistance levels for Pasteurella multocida differed by farm, and several antibiotics were not effective. The results of this study imply that antimicrobial susceptibility tests are highly recommended before administration.

This study aims to determine the difference in preferred horticultural plants, horticultural activities, and stress-coping strategies based on Enneagram centers of intelligence in middle-aged women. Data from a convenience sample of 206 middle-aged women were analyzed using IBM SPSS (V. 25.0). The results indicated a statistically significant difference in preferred horticulture plants depending on the Enneagram centers of intelligence. The results also suggested that head- and heart-centered respondents preferred body-centered respondents preferred “foliage plants.” The second most preferred horticultural activities showed no statistically significant difference among the head, heart, and body. However, all three centers showed the strongest preference for “enjoying” among various horticultural activities, and the second most preferred activities differed as “harvesting” among head- and body-centered respondents, and “decorating” among heart-centered respondents. Moreover, a difference in stress-coping strategies was also observed. The head-centered respondents used the “problem-focused” strategy, heart-centered respondents used “hopeful thinking,” and the body-centered respondents used “pursuit of social support.” Thus, these results can be used as baseline data for developing therapeutic horticultural activities while considering the attributes of middle-aged women.

This study was conducted to provide basic data on controlling boring insect pests by identifying the damage and the occurrence ecology of beetles, which are boring insect pests that seriously damage Castanea crenata, an income tree of mountain farms. By location, the damage rate was 62.5% at the foot of mountain, 61.4% at the mountain range, and 61.3% at the mountain peak. By region, the damage rate was the highest in Hapcheon at 65.3%, followed by 62.5% at Hadong and 58.1% at Jinju. The total number of adult fables was 171, followed by 49.1% (84) for Moechotypa diphysis, 39.2% (67) for Batocera lineolata, and 11.7% (20) for Anoplophora malasiaca. As for the male and female ratio of imago, Moechotypa diphysis had 51.2% (43) female and 48.4% (41) male. The percentage of emergence of beetles, which are boring insect pests appearing in the specimen collected by the survey area, was 46.0% in Hapcheon, 32.9% in Hadong, and 21.1% in Jinju. As for the prevalence of beetles, Batocera lineolata appeared from June 22 which is the first day of the emergence period to August 30, and the amount of emergence by the prevalence period was 3.0% in June, 46.2% in July, and 47.8% in August. Anoplophora malasiaca appeared from May 20 to July 20, and the amount of emergence by prevalence period was 5.0% in May, 10.0% in June, and 75.0% in July. Moechotypa diphysis first appeared from August 10 to September 30, and the amount of emergence by prevalence period was 1.2% in July, 51.2% in August, and 46.4% in September. As for the number of eggs laid by beetles, Anoplophora malasiaca laid 90±6, Batocera lineolate laid 75±5, and Moechotypa diphysis laid 60±6.

인터넷과 빅데이터 시대에 혼돈을 기반으로 한 디지털 영상암호화 알고리즘에 대한 연구는 정보보안 분야 의 연구 핫스팟 중 하나가 되었다. 기존의 텍스트 데이터에 비해 디지털 이미지는 데이터의 양이 많고 중복 성이 높은 특성을 가지고 있습니다. DES, AES 및 RSA와 같은 기존 방법은 디지털 이미지 암호화 요구 사 항을 충족할 수 없습니다. 초기 민감도와 고유한 임의성 때문에 카오스는 암호화의 혼란 및 확산 개념과 매 우 일치하므로 카오스 이미지 암호화에 대한 연구가 빠르게 발전했습니다. 본 논문에서는 주로 혼돈 영상 암호화에 대한 연구를 수행한다. 평문 영상에 대한 키 의존성을 목표로 비트 평면과 개선된 로지스틱 맵 기 반 영상 암호화 알고리즘을 제안한다. 알고리즘은 스크램블된 일반 텍스트 이미지의 낮은 4비트 비트 매트 릭스를 개선된 물류 맵의 제어 매개변수로 사용하여 일반 텍스트 이미지에 대한 키의 종속성을 높이고 놀 라운 일회성 비밀 특성을 갖습니다. 서로 다른 비트의 이미지 정보에 따라 서로 다른 강도의 암호화 알고리 즘을 설계하여 알고리즘의 효율성을 높입니다. 영상 암호화의 확산 모드를 목표로 하이퍼 카오스 시스템과 블록 모듈로 기반의 영상 암호화 알고리즘을 제안한다. 알고리즘은 하이퍼 카오스 시스템을 사용하여 카오 스 시퀀스를 반복적으로 생성하고, 암호화된 픽셀 주위에 임의의 이미지 블록을 분할하고, 블록의 픽셀 값 에 대해 적분 모듈로 연산을 수행하여 암호화된 픽셀 회색 값을 얻음으로써 확산 범위를 효과적으로 확장 하고 확산의 무작위성을 향상시킵니다.

The canine parvovirus (CPV) causes clinical signs, such as severe enteritis, dehydration, diarrhea, vomiting, leukopenia, and hair loss, which may lead to death. Vaccination is still the most important approach, as no specific treatment exists to prevent CPV. Monoclonal antibodies are valuable tools to study the pathogenic mechanisms of CPV and develop effective diagnostic reagents and pharmaceuticals. In this study, two monoclonal antibodies (MAbs) against CPV-2a were obtained through hybridoma technology by fusing myeloma cells and B cells from the spleens of mice immunized with CPV type 2a (CPV-2a). Two MAbs (CPV-330 and CPV-620) were studied on the reactivity of vaccine (CPV-2a) and field strains (CPV-new 2a, -2b, and -2c) by indirect immunofluorescence (IFA), hemagglutination inhibition test (HI), virus neutralization test (VN), and inhibition of virus growth test. Two MAbs showed similar antibody titers for HI and VN. On the other hand, CPV-330 inhibited the viral replication in Crandell-Rees Feline Kidney (CRFK) cells better than CPV-620. These CPV MAbs may provide valuable biological reagents to study the CPV pathogenic mechanisms and work as therapeutic antibodies.

자기공명영상장치(magnetic resonance, MR)/양전자 방출 단층촬영 장치(positron emission tomography, PET)는 두 가지 의료장치가 결합한 하이브리드 시스템으로써 MR의 해부학적 정보와 PET의 기능적 정보를 동시에 획득할 수 있는 최신 의료장치이다. 일반적으로 MR/PET의 우수한 팬텀 영상의 질 획득과 평가를 위하여 팬텀 내에 전기전도도가 낮은 액체 물질과 방사성동위원소를 주입하고, UTE MR 펄스 시퀀스를 적용한 감쇠 보정된 PET 영상을 획득한다. 본 연구의 목적은 MR/PET 전용 팬텀에서 물 대체물질로써 NaCl과 NaCl+NiSO4 물질에 따른 UTE MR 펄스 시퀀스를 획득하고, 감쇠 보정된 PET 영상의 질을 평가하고자 한다. 정량적 분석을 위하여 대조도 회복비(contrast recovery, CR), 신호대잡 음비(signal to noise ratio, SNR), 변동 계수(coefficient of variation, COV)를 적용하였다. NaCl 물질 기반 UTE MR 펄스 시퀀스를 적용한 PET 영상의 질이 CR은 1.38배, SNR은 1.18배가 증가하였고, COV는 1.18배 감소함을 확인할 수 있었다. 결론적으로, MR/PET 전용 팬텀을 활용한 신호의 획득 가능성을 확인하였고, UTE MR 펄스 시퀀스는 해부학 적 정보와 PET 영상의 질 향상에 필수적임을 확인할 수 있었다.