Methyl bromide (MB) has been still routinely used in quarantine fumigation on imported citrus, although there had been issuing chronic inhalation toxicity to fumigators and related workers as well as phytotoxic damages after fumigation. Ethyl formate (EF), is the only option to replace MB in terms of its safety for consumers (food additive and naturally occurred) and worker with higher threshold level limit (TLV = 100 ppm). Its application technology also provide cost effectiveness, good commercial practice in terms of application time (< 10 min) for 40 ft container. The replacement of MB with EF is recommended not to fumigate with hazardous and phasing-out MB on imported oranges.

The fumigation toxicity of carbonyl sulfide to T. castaneum as a storage grain pest was evaluated. Carbonyl sulfide (COS) is registered in Australia for microorganism present in soil, root and fertilizer. the fumigation activity of carbonyl sulfide was investigated in 12 L desiccator for 24 h exposure to eggs, larvae, pupae, adults of T. castaneum. Eggs and pupae were showed 87.3% and 95.6% mortality for 25 mg/L of COS, respectively. Larvae and adults were investigated with 80.0% and 100.0% mortality at 15 mg/L treatment, respectively. Therefore, the eggs of T. castaneum showed the highest tolerance to COS.

The fumigation activity of phosphine (PH3) to T. castaneum as a storage grain pest was evaluated. The lethal concentration time (LCT) value of each developmental stage (egg, early larva, late larva, pupa and adult) of T. castaneum was analyzed in 12 L desiccator. At the T. castaneum larva stages, exposure for 4 h showed low LCT value, especially in early stage larvae (LCT99 = 0.32 mg·h/L) which is very high susceptibility to PH3. However, T. castaneum eggs were observed very high tolerance to PH3 at LCT99 77.47 mg·h/L. Therefore, the fumigant activity of PH3 against T. castaneum can be found to be significantly different depending on developmental stage.

수노랑나비(Chitoria ulupi (Doherty, 1889))는 한반도 전역에 국지적으로 분포하는 종으로, 국외의 경우 인도, 미얀마, 라오스, 중국, 대만 등에 서식하는 것으로 알려져 있다. 이 종에 대한 생활사는 김과 서(2012)에 의해 확인된 유생기와 번데기의 형태 등이 일부 보고 된 것 이외에는 알려진 바가 없다. 본 연구에서는 한국산 수노랑나비의 생태적 습성(성충 산란행동, 점유행동, 짝짓기, 먹이습성 등)과 미성숙단계 (알, 유충, 번데기)의 형태적 특징 등 생활사 전반에 대하여 보고 하고자 한다.

친환경 벼 재배지역을 중심으로 점차 피해가 확대되는 먹노린재(Scotinophara lurida) 방제기술 개발을 위하여 전남지역에서 발생생태 및 온도의존 발육모형을 조사하였다. 2019년 봄 전남 6개지역에서 먹노린재의 월동후 생존율을 조사한 결과 총 153마리중 104마리가 생존하여 67.6%의 생존율을 보였다. 먹노린재는 벼 이앙 후 6월 중하순부터 월동성충이 발생하기 시작하여 7월 상중순에 산란하고 8월 중하순에 1세대 성충이 발생한다. 온도 의존 발육모형을 구하기 위하여 18, 21, 24, 27, 30℃ 14L:10D 조건의 항온기에서 온도별, 발육단계별 발육기간을 조사하였다. 18, 21, 24, 27, 30℃에서 알에서 성충이 될 때까지 소요기간은 각각 119.8, 73.1, 53.5, 39.4, 82.0일로 27℃에서 가장 발육이 잘 되었다. Excell 프로그램을 이용하여 온도와 발육속도와의 관계를 2차 다항식으로 분석하여 회귀곡선을 얻었고 이를 근거로 발육단계별 발육영점온도와 유효적산온도를 산출하였다. 먹노린재의 알에서부터 5령 약충까지의 발육영점온도는 17.9℃, 유효적산온도는 380.2일도였다.

Honey bees are affected by a variety of factors, so they have to be thoroughly managed according to their lifestyle. The activity of the honey bee foragers represent an important parameter of the hive state. Here, the real-time and automatic monitoring system using dual infrared sensors was applied for counting the foraging activity of honey bees based on ICT. According to this study, this system is very accurate with a relative error of 3.98% / 4.43% compared to manual counting through video analysis. This system showed the scalability of the system through the internal and external temperature sensors connected through the main board and BLE module. Furthermore, the data measured through this system for one month were analyzed, the monthly average foraging activity and the number of lost foragers were measured (1.88% of outgoing bees), and at the same time, the foraging patterns according to the changes of temperature and time were analyzed. This study suggests that the development of apicultural, scientific and educational materials with more powerful real-time monitoring tools through expansion of a complex monitoring system and big data accumulation.

경북 영양지역 고추 비가림하우스 재배지에서 2016년부터 2018년까지 주요 해충의 발생양상을 조사하였다. 발생조사는 영양읍, 청기면, 일월면을 중심으로 임의 10개 포장을 선정하여 4월부터 10월까지 실시하였다. 총채벌레류는 5월 중순, 6월 중순, 7월 상순∼중순, 9월 중순∼9월 하순에 걸쳐 총 4회의 발생최성기를 보였고 꽃노랑총채벌레가 84.8%로 우점하였다. 총채벌레 피해과율은 조사연도에 따라 0.8~7.3%로 조사되었다. 담배나방 유충에 의한 과실피해 는 7월 상순, 8월 중순, 9월 중순~9월 하순에 많이 발생하였다. 진딧물류는 5월 중순～5월 하순, 7월 중순∼8월 하순에 걸쳐 연 2회의 발생최성기를 보였다. 가루이류는 8월 중순～8월 하순, 9월 하순～10월 상순에 걸쳐 연 2회의 발생최성기를 나타냈으며, 담배가루이가 98.1%, 온실가루이가 1.9%로 담배가루이가 우점종인 것으로 확인되었다.

Riptortus pedestris is a polyphagous species and an economically important insect pest of soybean. We investigated the development time, survivorship, longevity and fecundity of R. pedestris at five different temperatures. Life table analysis is the prime tool in population dynamics and pest management. We applied the computer programs – TWOSEX-MSChart and TIMING-MSChart - to analyze our data. We obtained the population parameters – adult preoviposition period (APOP), total preoviposition period (TPOP), oviposition days, eggs per reproductive female, first age of survival rate <50%, proportion of male and female individuals and life table parameters (net reproductive rate, intrinsic rate of increase, finite rate of increase, and mean generation time) - and population projection using two computer programs.

The study conducted to understand the annual phenology of hemipteran insect pests including Halyomorpha halys, Nezara antennata, Plautia stali, and Riptortus pedestris in Jeju, Korea, 2017 and 2018. We investigated the population density of them at three locations for two years. The commercial and fish trap were used for monitoring. We compare the seasonal occurrence among species and sampling locations. The repeated measured ANOVA was used to explain the difference of seasonal occurrence among locations and sampling time. There was difference the first adult catch and the peak occurrence of species among sampling sites.

A tick survey was conducted to monitor ticks using tick traps attached dry ice and a flagging method at each four sites in Busan, Ulju, and Geoje areas of Gyeongnam Province from April to November, 2018. Haemaphysalis longicornis was the most frequently collected, representing 99.8% in Ulju. A total of 27,247 ticks were collected as H. longicornis (Trap Index; TI 283.2), H. flava (TI 0.3), and Ix. nipponensis (TI 0.3). In the collection environment, a total number of 15,672, 5,798, 4,876, and 901 ticks were collected from glassland, tump, tomb, and mountain path, respectively. On the other hand, H. longicornis and Ix. nipponensis were collected with flaggings at the four sites in Ulju, Busan, and Geoje in May and June, 2018. A total of 1,517 ticks collected H. longicornis (Flagging Index; FI 9.3) and Ix. nipponensis (FI 0.1) in Ulju. Haemaphysalis longicornis were collected 2,183 (FI 13.6) and 1,596 (FI 10.0) in Geoje and Busan, respectively. In the results of the isolation of Severe Fever with Thrombocytopenia Syndrome (SFTS) from the ticks, no pathogens detected from RNA of 557 pools of the ticks using a Polymerase Chain Reaction method in 2018.

본 연구의 조사지역인 갑천은 충청남도 금산군·논산시와 대전광역시를 북류하고 금강으로 흘러드는 도심하천이다. 광대한 갈대 군락과 초원, 버드나무 등 다양한 식생이 분포하며 크고 작은 자연 소택지, 늪, 웅덩이 등의 형태로 자연환경이 잘 보존되어 도시생태계의 주요 기능을 담당하고 있다. 본 연구에서 갑천 일대의 육상곤충류를 대상으로 4월 11~13일, 6월 11~12일, 8월 20~22일 조사를 수행하였다. 조사방법은 직접찾기와 쓸어잡기로, 총 9개 지점으로 나누어 군집 분석하였다. 조사 결과 공단구역인 Site3의 다양성지수가 0.16으로 매우 낮았으며 도심구역인 Site4와 Site7이 각각 다양성지수 2.92와 2.41로 타구역에 비해 낮았다. 반면 산림구역인 Site1, Site8 그리고 Site9는 모두 다양성지수가 3이상으로 높은 편에 속했다.

본 조사지인 돌리네 습지보호지역은 경북 문경시 산북면 굴봉산 정상부에 위치하였으며, 물이 고이기 힘든 돌리네(doline) 지형에 형성된 희귀 습지이다. 뿐만 아니라 멸종위기종과 법적보호종이 분포하는 등 생물다양성 또한 매우 풍부한 것으로 평가되고 있다. 그러나 습지보호지역 주변으로 과수원 등 경작지가 형성되어 있어, 관리 및 보전을 위한 조사 및 대책이 필요하다. 따라서 본 조사에서는 습지보호지역의 체계적인 관리를 위하여 돌리네 습지의 곤충상을 조사하였다. 조사 방법에는 곤충의 다양한 습성과 서식환경을 고려하여, Searching, Sweeping, Pitfall trap, Bucket trap으로 총 4가지의 채집방법으로 조사를 실시하였다. 그 결과 총 11목 82과 188속 237종이 확인되었으며, 이중 딱정벌레목이 가장 많이 출현하였다. 특이사항으로 환경부지정종 51종(중복포함)이 조사되었으며, 그중 멸종위 기종 1종이 확인되었다. 본 연구를 통해 돌리네 습지보호지역의 곤충상을 파악하고 관리 및 보전에 대한 기초자료를 제공하고자 한다.

The Celyphidae, commonly known as beetle flies or beetle-backed flies, are a family of Diptera. The scutellum is enlarged and forming a protective shell over the abdomen, giving them a beetle-like appearance. Species in the family are mostly found in tropical region, especially in South East Asia. Records of North East Asia are rare, in China, some species are found in Southern region. In Japan, few species found in Okinawa region, but only one record of Celyphidae near in mainland area. In this research we firstly record specimen of genus Spaniocelyphus sp., from Korea stored in National Science Museum Korea and Kyongpook National University with comparison of specimen from Tsushima island, Nagasaki prefecture stored in Osaka Natural History Museum.

The leafhopper family Cicadellidae (Hemiptera: Auchenorrhyncha) is plant feeders, with mouthparts adapted for sucking. Almost of the them cause injuries of destruction to plants, including fruit trees and grain crops, and can be vectors of plant diseases. Therefor, Cicadellidae is one of the most important pests in the world. However, their identifications are very difficult because of small size and morphological similarities, and their exact biological information such as host plants, life cycle and distribution are not clear for most species. So, we provide and integrated biological database that present accurate diagnostic methods and ecological infromation.

Drosophila suzukii (Matsumura), commonly called the Spotted Wing Drosophila (SWD), originally from South Asia, is a major pest in orchards. Recently, chemical control was applied to prevent the SWD in cultivated fields, but it was not much effective because D. suzukii tended to avoid place where insecticide was treated, to reproduce in wild field, and then to recolonize neighboring cultivated fields. In contrast to chemical control, biological control can reduce these problems by using natural enemies who attack the D. suzukii. Some species of genus Asobara (Hymenoptera; Braconidae; Alysiinae) are parasitoids of Drosophila, and, In South Korea, there are 3 species recorded parasitizing in D. suzukii. In this study, we used morphological characters and mitochondrial COI gene to identify Asobara species. As a result, one species was found as a possible parasitoid of D. suzukii.

Origin of the red imported fire ant (RIFA, Solenopsis invicta) is South America, a tropical climate region. It has settled in the invasive area, causing various problems. In recent, colonies of Solenopsis invicta were discovered in Busan in 2017, and then in Busan, Pyongtaek, Incheon, Daegu and Ansan in 2018. This study aims to validate 31 microsatlite loci referred from previous study for tracing origin of Solenopsis invicta colonies. We tried to test previously developed microsatellite loci so that we establish the protocol for molecular epidemiology of RIFA. We applied 31 microsatellite loci to DNA samples from the RIFA colonies found in Texas and Florida, USA. We will present 31 markers were divided into three injections and the amplification rate for each marker was shown as follows.

In the 21st century, the risk of the exotic pest being invaded is increasing due to the rise of trading activities. Oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae), has been considered to very destructive invader as it is highly intrusive and has a wide host range. In this study, 2,299 samples were collected from 88 locations in 12 countries by quarantine and investigation. Among them, 608 individuals (including B. correcta) were used for COI DNA barcoding analysis based on Neighbor Joining method with P-distance model. Population genetics analysis was conducted for 510 individuals selected from 47 locations of 12 Southeast Asian countries using 15 microsatellite loci. The barcoding analysis resulted in that any clade was not clustered according to a geographical isolation but indicated geographically mixed populations. Population genetics analysis showed shared genetic structure between neighboring countries across borders. Genetic structure of most Korean quarantine groups was more similar to that of Taiwan, China and Thailand in the order of appearance than other countries.

The morphological identification of alate aphids needs reliable and appropriate methods such as morphometrics. In previous studies, the seven species which aphids have been known as migrating to potato had been separated into genus levels using specific points of forewing vein (Landmarks). Adding the number of secondary sensillum of antennae, setae of abdomen, etc., we experimented to separate four species of genus Aphis (Aphis craccivora, Aphis fabae, Aphis gossypii, Aphis spiraecola). After taking each of 25X twenty photographies of four species, we calculated and measured coordinates of landmarks, the number of secondary sensillum of antennae, dorsal setae of 8th abdominal segment, anterior/posterior genital plate setae and cauda, and then performed the principal component analysis using PAST3 and the canonical variate analysis using MORPHO J, only with a landmarks. As results, in PCA, Aphis gossypii has been separated, but the others are overlapping. In CVA, all species of aphis has been separated.

Wildfire, which may effect on the spatial distribution of many insects, is one of important disturbances in forests. Because Monochamus beetles are known as vector insects of pine wood nematodes in Korea and burned pine trees can be used as oviposition habitats for Monochamus beetles, we investigated the changes in spatial and temporal distributions of M. alternatus and M. saltuarius across fire severity classes. We also studied other saproxylic beetles, such as cerambycid, scolytid, and bark beetles, which are potential insect pests for pine trees. Study area was classified by fire severity, such as unburned, light (<25% burned), moderate (25~75%), and severe (>75%). Spatial distribution of saproxylic beetles were significantly related to the fire severity.

An investigation on the habitation of Callipogon relictus Semenov was carried out in Gwangneung forest, Korea and Ussurisky nature reserve, Russia. During the investigation period (2007–2018), a total of 25 emergence holes, 10 pupal chambers, 10 adults, and two larvae of C. relictus were identified in Gwangneung forest. In this presentation, we provide biological information on habitation of C. relictus in Gwangneung forest by comparison and combination of survey results in Ussurisky nature reserve, in 2015 and 2018. In addition, the distribution range of C. relictus is also reviewed in the Korean Peninsula with a new location.