We conduct BVRI and R band photometric observations of asteroid (5247) Krylov from January 2016 to April 2016 for 51 nights using the Korea Microlensing Telescope Network (KMTNet). The color indices of (5247) Krylov at the light curve maxima are determined as B - V = 0:841  0:035, V -R = 0:4180:031, and V -I = 0:8710:031 where the phase angle is 14.1◦. They are acquired after the standardization of BVRI instrumental measurements using the ensemble normalization technique. Based on the color indices, (5247) Krylov is classied as a S-type asteroid. Double periods, that is, a primary period P1 = 82:188  0:013 h and a secondary period P2 = 67:13  0:20 h are identied from period searches of its R band light curve. The light curve phases with P1 and this indicate that it is a typical Non-Principal Axis (NPA) asteroid. We discuss the possible causes of its NPA rotation.

Metformin (1,1-dimethylbiguanide hydrochloride), derived from French lilac (Galega officinalis), is a first-line anti-diabetic drug prescribed for patients with type 2 diabetes. However, the role of metformin in odontoblastic cell differentiation is still unclear. This study therefore undertook to examine the effect of metformin on regulating odontoblast differentiation in MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells. As compared to controls, metformin significantly accelerated the mineralization, significantly increased and accelerated the expressions of ALP and Col I mRNAs, and significantly increased the accelerated expressions of DSPP and DMP-1 mRNAs, during differentiation of MDPC-23 cells. There was no alteration in cell proliferation of MDPC-23 cells, on exposure to metformin. These results suggest that the effect of metformin on MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells, facilitates the odontoblast differentiation and mineralization, without altering the cell proliferation.

In this study, magnetite (Fe3O4) nanoparticles were electrochemically synthesized in an aqueous electrolyte at a given potential of -1.3 V for 180 s. Scanning electron microscopy revealed that dendrite-like Fe3O4 nanoparticles with a mean size of < 80 nm were electrodeposited on a glassy carbon electrode (GCE). The Fe3O4/GCE was utilized for sensing chloramphenicol (CAP) by cyclic voltammetry and square wave voltammetry. A reduction peak of CAP at the Fe3O4/GCE was observed at 0.62 V, whereas the uncoated GCE exhibited a very small response compared to that of the Fe3O4/GCE. The electrocatalytic ability of Fe3O4 was mainly attributed to the formation of Fe(VI) during the anodic scan, and its reduction to Fe(III) on the cathodic scan facilitated the sensing of CAP. The effects of pH and scan rate were measured to determine the optimum conditions at which the Fe3O4/GCE exhibited the highest sensitivity with a lower detection limit. The reduction current for CAP was proportional to its concentration under optimized conditions in a range of 0.09-47 μM with a correlation coefficient of 0.9919 and a limit of detection of 0.09 μM (S/N=3). Moreover, the fabricated sensor exhibited anti-interference ability towards 4-nitrophenol, thiamphenicol, and 4-nitrobenzamide. The developed electrochemical sensor is a cost effective, reliable, and straightforward approach for the electrochemical determination of CAP in real time applications.

The ability of plants to endure environmental stress factors, which are going to be more severe due to global warming, is important especially for forest plants. Because obtain trait of resistance to temperature using conventional breeding for woody plants is a time consuming way. In this study, chloroplast-localized OsHSP26 gene was overexpressed in Populus alba L. to breed tolerant transgenic poplar to temperature stress. The plantlets of OsHSP26-overexpressed transgenic poplar showed more heathy phenotypic response than wild-type plants under both prolonged low- and high-temperature stress. While the SPAD value, which refers chlorophyll content, in wild-type plants decreased depending on the exposure time to the temperature stress, higher SPAD value were shown in the transgenic plants. The contents of total phenolic compounds in the transgenic plants were lower than those of the wild-type plants, and not significantly changed except in the treatment of prolonged low-temperature. However, the total flavonoids contents of the transgenic plants were dramatically increased under prolonged temperature stress. The DPPH scavenging activities of the transgenic plants were higher than those of the wild-type plants under temperature stress. Consequently, it was revealed that overexpressing OsHSP26 allow for P. alba to be tolerant to temperature stress.

The purpose of this study is to investigate and characterize the visual preferences of Rural Housing Standard Plans, which are expected to increase in demand as the number of households grows, and the effects of those characterizations on people’s choices in selecting home plans. In this study, Munsell values were extracted and analyzed using color analysis, and I.R.I image adjective analysis was performed to identify the color characteristics of each model type. To analyze the effect of the most influential colors among the visual elements in the plans, questionnaires were carried out according to whether color was present or not. There was a correlation between pre-selected frequency of preference and preference of visual preference. The study tallied the number of downloads for each model provided for the site of the farmers’ village and applied a t-test to confirm whether there was a difference in visual preferences. The results were as follows. First, the colors of Rural Housing Standard Plans were N and YR series, and the image analysis results were “Oriental” and “Rural”. Second, analyzing the number of model downloads and visual preferences on the homepage (www.returnfarm.com). Third, in the results of visual preference, the wooden house ranked 2.98-5.13 and the reinforced concrete house ranked 3.68-4.48. Fourth, comparative analysis of selection frequency and visual preference showed that the rankings of the top-ranked plans were similar; all were wooden houses and had similar roof forms. Fifth, the t-test conducted to analyze preferences in the visual elements of the standard design model of rural housing revealed significant differences in preferences for the color, roof form, and size, but no statistically significant differences (at .111) between preferences for structural wooden and reinforced concrete. The reason for this was that the types of wooden and reinforced concrete were not clearly distinguished from each other by photographs. Therefore, analysis suggested that the visual factors influenced the preference of Rural Housing Standard design model. In conclusion, it is necessary to present a model considering visual factors when providing models to urban and rural residents.

Effective estrus detection and artificial insemination (AI) are necessary for profitable management of dairy herd. In current study, 45 crossbred lactating cows have been selected with the complaint of unobserved oestrus for more than sixty days postpartum. All cows had functional corpus luteum as examined by transrectal ultrasonography. Cows were treated with PGF2α analogue and AI was performed with observed oestrus and then single dose of GnRH was administered. Similar synchronization protocol has been repeated after 14 days in cows that did not repose to first treatment. Remaining cows received additional PGF2α after 14 days of second treatment and timed AI was performed following GnRH administration. Among 45 cows, 28.89% showed estrus after first treatment and 78.79% responded to second hormonal intervention. A higher conception rate (88.89% vs 26.66 and 72.72%) was observed in cows after triple administration of PGF2α and timed AI. We noticed a significant differences in body condition score (BCS, 1～5 scale), postpartum period, and daily milk production between cows that either responded of non-responded following first and second hormonal treatment. In addition, there was a significant positive correlation between daily milk production and BCS, age and postpartum days, milk production and estrus/BCS, and milk production/BCS/estrus and conception rate. Depending upon the findings we conclude that hormonal intervention with PGF2α and GnRH enhances postpartum ovarian cyclicity and help decreasing the days open of dairy herd. Therefore, this finding might provide an excellent guideline for target breeding system for profitable dairy herd management.

Abstract2)The incidents of garbage dumplings in 2004 and the report on giant castella are the examples that shows how big the influence of broadcasting on the industry is. There were discussions on the importance of securing the objectivity of broadcasting, however, the existing related researches have lacked the analysis of actual proof for the influence of broadcasting contents, and as that of the law and system was confined to theoretical arguments, there were not enough suggestions for realistic alternatives. In this paper, we will examine the influence of broadcasting contents on the food industry through an analysis of actual proof, and propose alternatives in terms of the law and policy for securing the objectivity and fairness of broadcasting, to solve this problem.

Purpose: Diabetes is a metabolic disease that occurs when the pancreas does not produce enough insulin (Type I diabetes) or when the body does not effectively use the insulin (Type II diabetes). One of the most common complication of diabetes is diabetic retinopathy(DR). DR is a time-dependent disease and induces blindness. DR produces reactive oxygen species (ROS) and superoxide is mainly made from mitochondria electron chain and it is harmful to eyes. Methods: In this study, eyes were enucleated from glucose-immersed zebrafish which is good model to generate diabetes and then mitochondria were isolated to evaluate activities of mitochondria electron transfer complex. Activity of mitochondrial electron transfer complex in eyes was measured by UV-vis spectrometer. Results: After the glucose immersion, the amount of mitochondria in eyes was increased compared to non-glucose-immersed zebrafish. Mitochondrial complex I, II, III, and IV activities of glucose-immersed zebrafish was improved compared to non-glucose-immersed zebrafish. Conclusions: These results indicated that 3 days or 7 days glucose immersion on zebrafish to induce the early stage of diabetes on zebrafish. Zebrafish might contribute to metabolic compensatory mechanisms in eyes to restore their mitochondrial homeostasis on the early stage of diabetes.

Current marine navigational practice relies less on long-range visual marine signals such as lighthouses for reference purposes. This is due to the availability of Global Navigation Satellite Systems (GNSS), which are integrated with other navigational aids on ships. Therefore, the objective of this study is to review the function of Pisang Island lighthouse and to propose the most relevant use of Pisang Island for current navigational needs. The function of the lighthouse was reviewed according to the IALA Navigational Guide and the AIS data image. The result showed that the most suitable navigational use of the lighthouse is to act as a reference for Line of Position (LOP). The AIS data image indicated that mariners are not using Pisang Island lighthouse for LOP. The trend in the Straits of Malacca (SoM) was compared with the trend in the Straits of Dover, UK. The selected experts verified that LOP was not practised there. As a specific example, a tanker ship route in the South China Sea was used to further support that LOP was not practised. This evidence supported the view that Pisang Island lighthouse is less relevant for current navigational practice and does not directly support the coastal state VTS operation and the establishment of the marine electronic highway. Furthermore, the existing shore-based VTS radar has limitations on range and the detection of targets near Pisang Island. Therefore, this study proposes the establishment of a new radar station on Pisang Island at the existing site of the lighthouse. The proposed new radar station on Pisang Island will add to the existing coverage of the VTS radar, bridging the coverage gaps to overcome the weakness of the existing shore-based radar and improve the safety and security of marine navigation in the SoM.

Hart, William, Suh, Kyunghee, & Oh, Yeonglim. 2017. “OK in Emergency Dispatch Encounters”. The Sociolinguistic Journal of Korea 25(2), 1~28. This paper investigates the discourse marker OK in 911 emergency calls, paying special attention to how its use is embedded in the context of the request-offer sequence through which the caller and the dispatcher display distinctive orientations in terms of goals, rights, and responsibilities. In line with previous research, it was found that the use of this marker displays multifunctionality, which for callers is manifested in the function OK not only as a response token but also as an affiliative signal of cooperation in the acknowledgment and acceptance of directives and updates on the status of the emergency response. For dispatchers, this multifunctionality is found in the activation of a bidirectional looking function of the marker which orients backward in acknowledgment of information elicited and provided in previous turns, while simultaneously orienting forward in anticipation of an impending directive, request for information, or status update in the turn that follows.

Microsatellite SSR markers were developed and utilized to reveal the genetic diversity of 32 strains of Flammulina velutipes collected in Korea, China, and Japan. From SSR-enriched library, 490 white colonies were randomly selected and sequenced. In the 490 sequenced clones, 85 clones (17.35%) were redundant. Among the remaining 405 unique clones, 201 clones (49.6%) contained microsatellite sequences. As a result, 12 primer pairs produced reproducible polymorphic bands within diverse 4 strains and these selected markers were further characterized in 32 Flammulina velutipes strains. A total of 34 alleles were detected using the 12 markers, with an average of 3.42 alleles and the number of alleles ranged from two to seven per locus. The major allele frequency ranged from 0.42 (GB-FV-127) to 0.98 (GB-FV-166), and values for observed (HO) and expected (HE) heterozygosity ranged from 0.00 to 0.94 (mean = 0.18) and from 0.03 to 0.67 (mean = 0.32), respectively. SSR loci amplified with GB-FV-127 markers gave the highest polymorphism information content (PIC) of 0.61 and mean allele number of five, while for loci amplified with GB-FV-166 markers these values were the lowest, namely 0.03 and two. The mean PIC value (0.29) observed in the present study with average number of alleles (3.42). The genetic relationships among 32 Flammulina velutipes strains based on SSR data were generated by UPGMA cluster analysis. In conclusion, we succeeded in developing 12 polymorphic SSRs markers from SSR-enriched library of Flammulina velutipes. These SSRs are presently being used for phylogenic analysis and evaluation of genetic variations. In future, these SSR markers will be used in clarifying taxonomic relationships among the Flammulina velutipes.

‘Baekseung’, a new variety of Flammulina velutipes, was bred by mating two monokaryotic strains isolated from KMCC 4210 and KMCC 4216 in Mushroom Research Division, Baekseung ARES in 2016. Baekseung showed fast mycelial growth and high mycelial density on MEA (Malt Extract Agar) media for 7days of incubation. Spawn running period on the sawdust substrate required 30days at 25°C. The cultivation period and optimum temperature were 11±1 days at 14°C for primordia formation and 14±1 days at 7°C for fruiting body development. The length of pilei and stipes in Baekseung harvested in optimal stage exhibited 11.3±0.4㎜ and 89.2±7.1㎜ and Megumi harvested in optimal stage showed 8.2±1.0㎜ and 95.9±5.0㎜ respectively. Yield of Baekseung and Megumi strain grown of sawdust substrate was 153.7±12.5g and 150.5±29.7g per 850ml in bottle cultivation. The inferred tree exhibited the difference of phylogenetic relationship between the Korean white fruiting body strains such as Baekseung, Uri1ho, Fv-14-a-38, and Fv-14-a-51 and the Japanese white fruiting body strain Megumi.

In this study, we made population with high biological efficiency to investigate the complex genetic architecture of yield-related traits in A. bisporus. MB013 that crossed with bisp 15-p2 and bisp 34-p2 had high biological efficiency. 170 homokaryons was isolated with CAPS marker (PIN primer/HaeⅢ) from 1000 ISSs. And 100 BC1F1 hybrids obtained by crossing the homokaryons of MB013 with bisp15-p1. Parental line bisp 15-p2 and bisp 34-p2 and 100 homokaryons of MB013 will analyze genome sequencing. Also 100 BC1F1 hybrids will evaluate yield-related traits.