The citrus leaf miner (CLM) is a of important citrus pests in Jeju island, but it’s not clear about ecology of over-wintering in Jeju. We investigated the developmental stage to know how the CLM overwinters in 20 sites. And we investigated the mortality of larvae, pupae and adult stage of CLM in open citrus cultivated field December, 2017 to April 2018 and growth chamber conditions(from –10 to 5 ℃). As a result, no larvae and adult survived open field during winter. We concluded that the CLM usually overwinters as pupa during winter.

The citrus leaf miner (CLM) is an important citrus pest. A thorough understanding of the biology and population dynamics of CLM are essential for development of reliable pest population prediction system. We investigated the developmental periods of the species under ten constant temperature schemes (12.5℃~39℃). Furthermore, we established a development model of CLM, based on the result of a laboratory experiment. The immature developmental duration of CLM at constant temperatures were 63.5 days at 15℃, 23.9 days at 21℃, 15.6 days at 27℃, and 12.3 days at 33℃, showing statistically significant difference among temperature regimes. The lower threshold temperature and thermal constant were 11.3℃ and 243.7 DD, respectively, for immature development. In relation to temperature, non-linear development models were established for each developmental stage of CLM.

Honey production from approximately 1.7 million colonies owned by around 21 thousand Korean beekeepers was almost 36 thousand M/T in 2014. Honey farmers have mainly obtained commodities such as honey, beeswax, pollen and royal jelly. Honeybees gather the nectars and pollens from flowers of plants or trees. Bee pollen is rich in various nutrients and called as natural complete food. Bee pollens of Quercus acutissima, Actinidia arguta, Amorpha fruticosa, Oryza sativa, Chrysanthemum morifolium and Salix koreensis were mainly collected by Apis mellifera in Korea. But stiff pollen wall hinders dissolution of polysaccharides and lowers extraction efficiency. Thus, we measured antioxidant activity as DPPH radical scavenging assay system on six kinds of pollens inoculated with fungi to confirm the husk removal effect. The antioxidant activity was evaluated by measuring the free radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Briefly, extracts prepared from different extraction conditions were mixed with freshly prepared DPPH solution. After shaking, the reaction mixtures were stand for 30 min at room temperature in dark places. The radical scavenging activity was determined by measuring the absorbance at 517 nm. The relative radical scavenging activity (%) was calculated as [1-absorbance of solution with sample and DPPH / absorbance of solution with DPPH] x 100. The scavenger activities of Quercus acutissima, Actinidia arguta, Amorpha fruticosa, Oryza sativa, Chrysanthemum morifolium and Salix koreensis were 42.73±1.25, 8.98±1.46, 4.38±3.53, 36.09±0.74, 17.28±1.95 and 62.76±2.07 at 500㎕/㎖, respectively.

Bee pollen is rich in various nutrients and called as natural complete food. Bee pollens of acorn (Quercus acutissima) and actinidia (Actinidia arguta) are the most collected in Korea. But stiff pollen wall hinders dissolution of polysaccharides and lowers extraction efficiency. Thus, we measured antioxidant activity as DPPH radical scavenging and the total polyphenol content of pulverized and lyophilized actinidia pollens inoculated with fungi to confirm the husk removal effect. The total polyphenol content of actinidia pollen was highest in lyophilized pollen medium inoculated with Armillaria mellea, and was lowest in pollen inoculated with Lentinula edodes. Total polyphenol content of the lyophilized pollen was higher than that of the refined pollen and the pulverized pollen in actinidia pollen germinated with A. mellea. The total polyphenol content of the lyophilized actinidia pollen germinated with A. mellea was 1.4-fold higher than that extracted with water. Measurement of antioxidant activity using the DPPH (2, 2 diphenyl-1-picrylhydrazyl) free radical scavenging method showed that the lyophilized actinidia pollen germinated with A. mellea had the highest and that germinated with L. edodes was lowest in antioxidant activities. The lyophilized actinidia pollen germinated with A. mellea was 2 to 4 times higher than that extracted with water in the antioxidant activity of DPPH free radical scavenging. Many germinated cells were formed around pore of actinidia pollen inoculated with L. edodes, while those were formed at the end of hyphae derived from actinidia pollen inoculated with A. mellea.

Insects are among the most diverse groups of animals on the planet, representing more than half of all known living organisms. These insects are found in nearly every environment. Although humans regard certain insects as pests and attempt to control them using insecticides, most insects perform complex ecological functions, and provide either direct or indirect economic benefits to humans. Recently, the importance of insects used as food sources or as pets has increased in many countries, including Korea. In addition, several insects have a strong influence on people's emotion. Insect-mediated mental healthcare program is designed to help people who have disorders with physical, behavior and development. Children who have mental disorder, the experimental group that was provided with an insect-mediated mental healthcare program over a total of 8 sections, one section per week, 60 minutes per section, followed by pre-test and post-test. They responded to therapeutic effect after the completion of the program. Further research on the basis of this study is expected to help children with emotional therapy in other areas.

Hereditary dentin defects consists of dentin dysplasia(DD) and denti nogenesis imperfecta(Dr) ‘ The Dl associated with osteogenesis imperfecta has been classified as DI type 1. whereas isolated inherited defects have been categori zed as DI types II and III , However‘ whether DI type III should be considered a distinct phenotype 01' a variation of DI type 1I is debatable , Recent genetic findings have focused attention on the role of the dentin sialo phosphoprotein(DSPP) gene in the etiology of inherited defects of tooth dentin, We have identified novel mlltation( c,727G - > A, p,D243N) at the 243th codon of exon 4 of the DSPP gene in a Korean patient with DI type III The radiographic and histologic features of the patient revealed the classic phenotype of shell teeth These findings sllggest that DI type II and III are not separate diseases bllt rather the phenotypic variation 01' a s ingle disease

Background : Ginseng is a perennial plant and damaged by various diseases and insect pests. The damage lowers harvest and quality. Accordingly, a chemical control system was made with the aim of effectively control gray mold, anthrax, and spotting disease which occur during growth period. fungicide resistance of gray mold rot was examined to find out whether the chemical control system can be used over the long term. Methods and Results : This experiment was conducted in three areas including Geumsan-gun, Yesan-gun, and Sejong city. The chemical control system was done to the plants of two years old or older. As for the order of the treatment, from the end of April to May Fludioxonil (A) and Pyraclostrobin (B) were used; from the mid-May to the end of July of growth stages Difenoconazole (C), Iminoctadinetris (albesilate) (D), Cyprodinil (E), Metconazole (F), Fluazinam (G) and Pyrimethanil (H); from August to September nonresidualizing polyoxinB + mancozeb (K); in November of hibernating period, Fenhexamid (I) and Carbendazim/diethofencarb (J) were used. As for the interval, from the end of April to the end of July the interval was 10 days, in August and September once a month, and twice in every ten days in November. The isolation of Botrytis cinerea for examination into mycelial growth inhibition rate was conducted to 4-year old ginseng in Geumsan, 5-year old in Yesan, and 3-year old in Sejong. In Geumsan, the mycelial growth inhibition rate to Botrytis cinerea was 75.5% - 100%. Every fungicides showed good rate of mycelial growth inhibition. The lowest rate was seen in fungicide B at 75.5%. K showed the prevention rate at 76.8%, D at 82.7%, and I at 82.2%, and other fungicides at 100%. In Sejong area, the hyphal prevention rate of Botrytis cinerea was 71.0% - 100%, indicating all fungicides show good rate of mycelial growth inhibition. The lowest rate was recorded by fungicide B at 71.1%, D at 81.1%, K at 85.4%, and I at 95.4%. Yesan area also showed similar results to those of Geumsan and Sejong. Conclusion : Botrytis cinerea was isolated from ginseng and mycelial growth inhibition effect was examined in concentration of 11 kinds of fungicides. In all three areas where chemical control system were applied, resistance was not found, suggesting that the chemical control system can be applied to control diseases of ginseng.

Background : Ginseng is a perennial plant and damaged by various diseases and insect pests. The damage lowers harvest and quality. In particular, gray mold rot occurs to plants of every year, growth duration, and hibernating stage, and decays leaves, stems, and roots, lowering the amount of harvest. This experiment was conducted with the aim of effectively preventing gray mold rot, and other major diseases such as anthrax and spotting disease, and establishing chemical control system. Methods and Results : This experiment was conducted in three areas including Geunsan-gun, Yesan-gun, and Sejong city. As for the procedure of medical treatment, from the end of April to early May of germination period, Fludioxonil and Pyraclostrobin were used. From the mid-May to the end of July of growth period, Difenoconazole, Iminoctadine tris (albesilate), Cyprodinil, Metconazole, Fluazinam, and Pyrimethanil were used. From August to September, non residualizing polyoxinB + mancozeb was used. In November of hibernating stage, Fenhexamid and Carbendazim/diethofencarb were used. As for the interval, from the end of April to the end of July the interval was 10 days, in August and September once a month, and twice in every ten days in November. The chemical control system effect was compared with conventional prevention. When chemical control system was used, stem spotting disease occurred at a lower rate of 0.34% than 1.2% of the conventional method. Leaf spotting disease occurred at a lower rate of 1.4% compared with 7.1% of the conventional method, and anthrax occurred at a lower rate by more than 10% than the conventional method. Stem gray mold rot occurred at a rate of 4.1% when the conventional method was used, but the rate stood at 5.3% in Geumsan, 8.9% in Yesan, 2.3% in Sejong when the prevention method was used, which suggest the chemical control system was effective. Conclusion : When chemical control system was applied to prevent major diseases of ginseng, spotting disease, anthrax, and gray mold occurred at lower rates compared with the case where the conventional method was used. The finding is that the chemical control system can be utilized to prevent major diseases of ginseng.

Background : This study was carried out to investigate the cytotoxicity in 9 extracts from 8 medicinal plants, such as leaf extract of Lonicera maackii (Llm), leaf extract of Platycarya strobilacea (Lps), flower extract of Fagopyrum dibortryis (Fdf), stem extract of Physostegia virginiana (Spv), root extract of Allium senescence (Ras), aerial part extract of Allium schoenoprasum (Aas), aerial part extract of Artemisia japonica var. manshurica (Aaj), stem extract of Caryopteris incana (Sci), and leaf extract of Caryopteris incana (Lci), on human cancer cell lines. Methods and Results : Dried plant extracts were granted from National Institute of Horticultural and Herbal Sciences. The extracts of each plant were dissolved in DMSO and stored in deep freeze at –20℃. The cell viabilities were examined by MTT assay. On SK-OV-3 cell line, Lps, Aas, Sci ans Lci showed dose-dependent cytotoxic effect. On A549 cell line, almost samples show dose-dependent cytotoxic effect, but especially Aaj showed relatively high cytotoxic effect. In case of HCT-15 cell line, Llm and Aas showed relatively high cytotoxic effect. Conclusion : These results suggested that Lonicera maackii, Platycarya strobilacea, Fagopyrum dibortryis, Physostegia virginiana, Allium senescence, Allium schoenoprasum, Artemisia japonica var. manshurica, and Caryopteris incana can be utilized as potential sources of anticancer agent due to their cytotoxicity.

Background : The purpose of this study was to evaluate methods to high quality food ramie rice cake, thereby increasing farm income. This study investigated the effects of different Split Application and Adapted Fertilizer Culture for Stable Production with Edible Leaf in Ramie (Boehmeria nivea L.). Methods and Results : Method of fertilizer application were conducted under three condition compose to standard application (N-P-K-Compost applied at 27-9-27-1,500 ㎏․ 10 a−1), 25% increased application (N-P-K-Compost applied at 34-11-34-1,500 ㎏․10 a−1), 50% increased application (N-P-K-Compost applied at 41-14-41-1,500 ㎏․ 10 a−1) cultivation. Compost and fused phosphate applied at 100% of basal fertilizer in March 25. The split application level of standard application (N-K) applied at 50% of basal fertilizer in March 25, 50% of top dressing were five times application (N-K) in May 4, July 5, September 5, October 5, 20% respectively. Transplanting with Yeongkwang varieties in April 12 by growing pot seedling for 35 days in 2011. Planting density were spaced 60 ㎝ apart in rows 25 ㎝ apart with rice straw cutting covered open cultivation. Plot design randomized block 3 repetition. Conclusion : Organic matter, available phosphate, K2O, CaO, and MgO of soil increased in all plots with the addition of fertilizers culture, specifically in 25% and 50% increased application. Growth characteristics by 50% of top dressing were five times application (N-K) in May 4, July 5, September 5, October 5, compared to standard application (N-K) applied at 100% basal fertilizer, aerial part as a result were so many such amount of growth as number of branch and leaf per plant, etc., Length, width of leaf and leaf colorimetry of aboveground part growth increment rising highest. Green leaf yields compared to standard fertilizer 100% application (1,344 kg․ 10 a−1). 25% and 50% increased application (N-K) applied at 50% basal fertilizer, 50% of top dressing were five times application increased by 9%, 17%. respectively.

Background : The purpose of this study is to investigate the effect of split application and adapted fertilization application cultivation in stable medicinal herbs production of Angelica acutiloba K. And it accomplished one’s purpose to GAP guide manual established standard method of cultivation technology, contribute to the enlargement of farm’s income. Methods and Results : Method of fertilization application were conducted under four condition compose to standard application (N-P-K-Compost: fermented mixed oil cake fertilizer applied at 14-10-10-600 ㎏․10 a−1cultivation. Compost and fused phosphate applied at 100% of basal fertilizer in April 14. The split application level of standard application (N-K) applied at 100%, 50%, 40%, 30% of basal fertilizer in April 14, 50%, 40% and 30% of top dressing were two, three times application (N-K) in June 1, July 15, August 15, 25% and 20% respectively. Sowing dates with Jangsung varieties in April 28 of the year 2015. Planting density were spaced 30 ㎝ apart in rows 15 ㎝ apart with black color non-woven cloth covering open cultivation by level row 90 ㎝. Plot design randomized block 3 repetition. Conclusion : Growth characteristics by 50%, 70% and 60% of top dressing were two, three times application (N-K) in June 1, July 15, August 15 compared to standard application (N-K) applied at 100% basal fertilizer, aerial part as a result were so many such amount of growth as number of leaf per plant, etc., Length of leaf and main root of aboveground, subterranean part growth increment rising highest. Dried roots yields compared to standard fertilizer 100%application (217 kg․10 a−1) treatment. 50%, 70% and 60% of top dressing were two, three times application (N-K) increased by 6%, 14%, and 18%. respectively.

Background : The purpose of this study was to evaluate methods to high quality medicinal vegetables, thereby increasing farm income. This study investigated the effects of different green manures crop and application culture stable foliage production in Peucedanum japonicum Thunberg. Methods and Results : Method of application with green manure crop were conducted under four condition compose to control (non application), single cropping in barley, hairy vetch and mixed cropping in hairy vetch (60%) + barley (40%)culture. Sowing dates of green manure crop were October 14 in 2014. Transplanting with Jeonam Yeosu varieties in Junel 28 by growing pot seedling for 45 days in 2015. Method of application were standard application (N-P-K-Compost applied at 20-12-10-600 ㎏․10 a−1). Plants were spaced 10 ㎝ apart in rows 10 ㎝ apart with open cultivation. Conclusions : The utilization effect of nitrogen supplied by green manure crop was highest with the hairy vetch by 21.1% in single cropping and hairy vetch + barley by 29.8% in mixed cropping. Organic matter, available phosphate, K2O, CaO, and MgO of soil increased in plots with the mixed cropping hairy vetch + barley culture. Non application < barley < hairy vetch < hairy vetch + barley in mixed cropping, aerial part as a result were so many such amount of growth as number of leaf per plant, etc., green leaf yields is untreated control plot (1,876 kg․10 a−1) compared to 7% from using barley culture, hairy vetch culture 10% higher and hairy vetch + barley culture increased to 15% of the most.

Background : As a part of ongoing research to elucidate and characterize anti-inflammatory nutraceuticals, six kinds of plant extracts (aerial part of Nepeta cataria, leaves of Lonicera maackii, leaves of Platycarya strobilacea, flower of Fagopyrum dibotrys, flowers and fruits of Solanum nigrum, stem of Physostegia virginiana) were tested for their ability to suppress inflammation. The anti-inflammatory has been studied in lipopolysaccharide (LPS)-stimulated RAW264.7 cells which cells synthesized nitric oxide (NO) from L-arginine by nitric oxide synthase (NOS). In this study, NO synthesis inhibitory activity of six kinds of plant extracts on LPS-stimulated RAW 264.7 mouse macrophages was evaluated. Methods and Results : Six kinds of plant extracts were parceled out from RDA (Rural Development Administration). RAW 264.7 cells (1.5×105 cells/well) were seeded onto 96-well plates with DMEM media containing 10% FBS and 1% antibiotics. The cells were pretreated with the extracts and LPS-stimulated cells for 24 h. Cellular NO production was stimulated by adding 1 μg/mL of LPS. After incubation, Griess reagent was used to determine NO production. Absorbance was measured at 520 nm by microplate reader. NO synthesis inhibitory activity potential of these extracts was evaluated by assessing NO production by LPS-stimulated RAW 264.7 cells in the presence. As a result, inhibition rate of NO production was about 40% of L. maackii, 33% of F. dibotrys, 23% of P. strobilacea and 17% of P. virginiana. Meanwhile, there was no significant results in aerial part of N. cataria and flowers and fruits of S. nigrum. Conclusion : From the above results, we be able to confirm that leaves of L. maackii and flower of F. dibotrys appeared dose-dependent NO synthesis inhibitory activity and leaves of P. strobilacea appeared NO synthesis inhibitory activity in low-concentration. As screening NO synthesis inhibition of six extracts, they may be a good candidate for delaying the progression of human inflammatory diseases and warrants further studies.

Background : The purpose of this study was to big strong and root in the disease, many high quality seed of Achyranthes japonica N. selection by fostering, expanding the spread on a farm and raw materials medicines of sources deployment for process is to contribute to increasing farm income. Methods and Results : Collected variety into the mass selection breeding method by head-to-row cultivation, native variety of Hwasoon and Jangheung Jeonnam, was conducted in 2007. Three individual selection in 2010 as it produces the 27 and five individual in 2008 and 2009. From 2010 to 2011, pure line isolation as it cultivated and one foundation group. Promising in 2012, select one system. Replicated yield trial for two years beginning in 2013 a result of the stem length, multiple No. of branches and providing high -quality “No. 1, Jeollanam-do” majority by assigning system name. Naju-si, Yeongam-gun, local excellence by implementing a local adaptability test recognized in two years. To breed a new variety named as a dwelling “ Woogang” of Achyranthes japonica N. the review by fostering december 2015 to be registered. Conclusions : Leaf type is the broad lancet, flowering time is new variety two days than Jangheung native variety delayed. New variety of stem length and main root length are check variety (89㎝, 13.5㎝) of 9㎝ and 2㎝ tall respectively. Seed yields has native variety as much as 35 % compared to the many into 43.5 kg/10a. The ecdysteroids contents of roots was 1.2 times higher compared to check variety (668.1ppm). The dried root yields of the new variety are 285kg/10a a many native variety than 20%.

Background : The purpose of this study is to investigate the effect of the ridge and mulching cultivation and adapted sowing(planting)time in stable medicinal herbs production of Lithospermum erythrorhizon Siebold et Zuc. And it accomplished one’s purpose to GAP guide manual established standard method of cultivation technology, contribute to the enlargement of farm’s income. Methods and Results : Method of cultivation type with the ridge and covering material were conducted under two condition compose to open cultivation (level row : 120㎝ ridging, black polyethylene mulching) and plastic greenhouse cultivation (high row : 90㎝ ridging, non-woven fabric mulching). Sowing with Daehong varieties in April 17, transplanting July 25 by growing pot seedling for 40 days in 2015. Planting density were spaced 30㎝ apart in inter-rows 10 ㎝ apart intra-row. Fertilizing level of N-P-K-Compost (fermented mixed oil cake fertilizer) applied at 6-4-6-300 ㎏․10 a−1, 60% of basal fertilizer in March 14, 40% of top dressing were four times application in April 14 ～July 15. Plot design randomized block 3 repetition. Conclusions : Compared to level ridging on black polyethylene mulching open cultivation for growing plastic greenhouse with the high ridging non woven mulching cultivation, seed yields havesting 1.4 times, the dried roots yields increased in 0.7 times. Also, Growth characteristics by sowing time in April 16. compared to planting time in July 25. Length of stem and main root of above-ground, subterranean part growth increment rising highest. Seed and dried roots yields with increased by 71%, 30%. respectively.

Background : The purpose of this study was to evaluate methods to high quality medicinal vegetables, thereby increasing farm income. This study investigated the effects of different organic fertilizer and applicated cultivation on stable foliage production in Peucedanum japonicum Thunberg. Methods and Results : method of application with organic fertilizer were conducted under nine condition compose to control(non application), N-P2O5-K2O (20-12-10)㎏․10 a−1, Hyphal incubation compost(HIC) applied at 851㎏․10 a−1, Hangawi organic compost(HOC) applied at 547㎏․10 a−1, Fermented mixed oil cake compost(FMOC) applied at 380㎏․10 a−1, 60% and 40% of basal fertilizer in March 12, top dressing were four application in April 12, May 12, June 13 and July 12, respectively. Sowing dates were March 23, 2015. Plants were spaced 15 ㎝ apart in rows 15 ㎝ apart with open cultivation. Conclusions : chemical fertilizers < hyphal incubation compost < hangawi organic compost < fermented mixed oil cake compost application, top dressing than 60% in order of 40% additional fertilizer, aerial part as a result were so many such amount of growth as number of leaf per plant, etc., Green leaf yields is untreated control plot (875 kg․10 a−1) compared to 12% from using chemical fertilizers, HIC application 18% higher, HOC application 20% higher, and FMOC application increased to 28% of the most.