Field experiments were conducted to investigate persistence of Chinese milk vetch(CMV) seed under naturally reseeded CMV-rice cropping systems during the period of 2007～2009. Persistence of the CMV seeds were evaluated in the naturally reseeded CMV-rice field on different tillage methods and soil depths based on the seed number recovered and the seed longevity in soil. Field observation in the naturally reseeded CMV rice field showed that as many as 917～2,185 CMV seeds m-2 were found from 0～15cm soil depth in the rotary tillage and 250～10,105 CMV seeds in minimum tillage rice cultivation in autumn. The recovered seed germinated 25～33%, 23～43% but still had high percentage of hard seed having 64～72% and 51～77%. Field experiment showed that the CMV seedling still emerged even after 2 years of continuous destructive killing of emerged CMV plant by rotary tillage in naturally reseeded CMV-rice field, indicating that CMV seeds persistent at least two years in rice field.

Experiments were conducted to investigated the change of primary dormancy and viability of Chinese milk vetch(CMV) seed buried in soil both as seed and pod with seeds under CMV-rice cropping system during the period of 2007～2009. The freshly harvested CMV seed alone and pods with seeds were buried in rice field at 0, 5 and 10cm depths and determined change of seed dormancy and viability at one to three month intervals for 10 months. The CMV seed had high dormancy of 95%, showing only 4～5% germination at the beginning in June but the seed dormancy was gradually broken at rice harvest time in autumn, showing 25 to 35% for seed and 55 to 61% for pod with seeds. The viability loss was faster in the seed than in the pod with seeds regardless of depths of placement in the soil base on decayed seeds. Also the seed placed on the soil surface lost viability faster than the 5～10 burial depths. However, appreciable number of CMV seeds still remained at even 4 months after burial in soil. These results indicate that seed dormancy was enable CMV plant to regenerate naturally from the remained soil seed bank at rice harvest time in autumn.

To better understanding the function of the luminal sub-organelles within the thylakoid network, we have carried out a systematical analysis and identification of the lumenal proteins in the thylakoid of wheat by using Tricine / 1D-PAGE, and LTQ-ESI-FTICR mass spectrometry followed by SWISS-PROT database searching. We isolation and fractionation these membrane from fully developed wheat leaves using a combination of differential and gradient centrifugation couple to high speed ultra-centrifuge. After collecting all proteins to eliminate possible same proteins, we estimated that there are 407 different proteins including chloroplast, chloroplast stroma, lumenal, and thylakoid membrane proteins excluding 20 proteins, which were identified in nucleus, cytoplasm and mitochondria. A combination of these three programs (PSORT, TargetP, and TMHMM) was found to provide a useful tool for evaluating chloroplast localization, transit peptide, transmembranes, and also could reveal possible alternative processing sites and dual targeting. Finally, we report also sub-cellular location specific protein interaction network using Cytoscape software, which provides further insight into the biochemical pathways of photosynthesis. The present work helps understanding photosynthesis process in wheat at the molecular level and provides a new overview of the biochemical machinery of the thylakoid in wheat.

Rice lax panicle (lax) mutant had defect in early inflorescence differentiation and lateral branch formation and exhibitedfewer numbers of rachis branches and spikelets in the panicle. The mutant also had abnormality in floral organ formation. Whereas, the spotted leaf 6 (spl6) mutant produced lesions in absence of pathogen. Analysis with a lax spl6 double mutant indicated both genes are controlled by single recessive factor and linked on chromosome 1 with 7 centiMorgan map distance. The lax gene was fine mapped within the 261.3 kb region between RM7594 and RM5389 that predicted 10 open reading frames (ORF) as candidate for lax gene. Sequencing of the candidates in the lax mutant revealed a substitution of nucleotide T by G corresponding to an amino acid substitution from serine (S) to alanine (A) at the 117th position within the coding region of the candidate ORF bhlh123. The intronless 1080 bp cDNA of LAX gene contains an ORF of 215 amino acids and encoded a basic helix-loop-helix transcription factor. Analysis of a 5.1 kb genomic fragment of the lax gene from homozygous dominant progeny which corresponded to indica cultivar resulted in a long deletion of 24 nucleotides in the upstream of LAX cDNA. (This research was supported by the National Research foundation of Korea, Grant 0070065).

Climate warming has the potential to deteriorate grain yield and quality of rice (Oryza sativa L.), offsetting the stimulative effects of elevating CO2. To know how the change in sink-source balances by reducing sink-size (RSS) may affect grain yield and quality of rice grown under various climate change scenarios, we conducted a temperature gradient chamber experiment with/without CO2 fumigation systems which were established in paddy field. Rice crops (cv. Ilmybyeo) were exposed to either ambient (396ppmV) or elevated CO2 of 673ppmV in three levels of air temperature [(Ta), local ambient Ta (24.8℃), 1.3℃ and 2.4℃ above ambient Ta] over whole seasons. Thus, the experiment was a 2×3 factorial design with three replicate plots of each CO2×Ta combination. At flowering, for two hills from each combination treatment total thirty (10 per each top, middle and basal parts of panicle) spikelets per panicle were removed with order of panicle appearance by scissors. This corresponded to a 25% reduction of total sink-size per hill. In ambient Ta and CO2 , grain yield decreased with RSS by 23.4%, approximately mirroring the reduced sink-size. With rising Ta, however, the yield reduction by RSS was significantly mitigated (-5.6% in 1.3℃ above ambient Ta), and the yield rather increased with RSS by 9.3% in 2.4℃ above ambient Ta. This was due primarily to the increased single grain mass with RSS. A similar response fashion of grain mass and yield with RSS to Ta was found in elevated CO2, but not CO2×Ta interaction. For brown rice, the fraction of normal rice was linearly reduced with rising Ta, ranging from 78.5～79.2% in local ambient Ta to 48.2～55.5% in 2.4℃ above ambient Ta over CO2 treatments. However, this deteriorative effect of rising Ta was significantly alleviated with RSS; the fractions of normal rice were a 81.9～84.1%, 75.9～77.2% and 64.0～66.3% in local ambient Ta, 1.3℃ and 2.4℃ above ambient Ta, respectively. The alleviative effect of RSS on rice quality was due mainly to the reduced immature rice, and was more conspicuous as Ta rises. These results suggest that current rice cultivars in Korea, at least cultivars tested in this experiment, will likely to be prone to source-limitation in the future projected warming with elevating CO2, and thereby will be needed a cultivar having either a greater source ability or a less sink size compared with current cultivars, in order to ensure a rice quality in the future warming conditions.

To know how interacting climate drivers may affect rice quality, we investigated physio-chemical properties of brown and milled rice. Rice crops (Oryza sativa L., cv. Ilmybyeo and Pyounganbyeo) were grown under either ambient [370ppmV (2008)/396ppmV (2009)] or elevated CO2 of 650ppmV (2008)/673ppmV (2009) in three levels of air temperature [(Ta), local ambient Ta [25.9℃ (2008)/24.8℃ (2009)], 1.3℃ and 2.4℃ above ambient Ta] over whole seasons, using six temperature gradient chambers established in paddy fields. Over 2 years, thus the experiments were a 2×3 factorial design with three replicate plots of each CO2×Ta combination. The fractions of normal brown rice were reduced with elevating CO2 by 8% (Ilmybyeo)～14% (Pyounganbyeo), and with rising Ta by 16% (+1.3 ℃)～27% (+2.4℃) in Ilmybyeo and by 27% (+1.3℃)～42% (+2.4℃) in Pyounganbyeo (p=0.015, 0.000, 0.059, 0.000 and 0.017 for CO2, Ta, CO2×Ta, cultivar and Ta×cultivar, respectively). With respect to immature rice, elevating CO2 increased milky-white rice, white-based rice and white-belly rice across cultivars. Warming also significantly increased all immature rice across cultivars, though no CO2×Ta interaction was observed. Over 2 years, the deteriorative effect of warming on brown rice quality was significantly greater in Pyounganbyeo than in Ilmybyeo. Across cultivars, protein contents of milled rice were decreased (c. 5～9%) with elevating CO2 but increased (c. 5%) with warming, though no CO2×Ta interaction was found (p=0.119). Elevating CO2 significantly increased whiteness of milled rice over cultivars but not amylose contents and gloss value of cooked rice, while warming had a strong affect these properties all related rice quality. Overall, our results suggest that warming and elevating CO2, in each alone or in combination, may have the potential to deteriorate physio-chemical properties of rice related to quality.

Since fuzzy controllers are nonlinear, it is more difficult to set the controller gains and to analyse the stability compared to conventional PID controllers. This paper proposes a fuzzy PD+I controller for tracking control which uses a linear fuzzy inference(product-sum-gravity) method based on a conventional linear PID controller. In this scheme the fuzzy PD+I controller works similar to the control performance as the linear PD plus I(PD+I) controller. Thus it is possible to analyse and design an fuzzy PD+I controller for given systems based on a linear fuzzy PD controller. The scaling factors tuning scheme, another topic of fuzzy controller design procedure, is also introduced in order to fine performance of the fuzzy PD+I controller. The scaling factors are adjusted by a real-coded genetic algorithm(RCGA) in off-line. The simulation results show the effectiveness of the proposed fuzzy PD+I controller for tracking control problems by comparing with the conventional PID controllers.

We developed a novel dicistronic system for the expression of target cDNA sequences in the milk of transgenic animals using goat beta-casein/hGH fusion construct, pGbc5.5hGH (Lee, 2006) and internal ribosome entry site (IRES) sequences of encephalomyocarditis virus (EMCV). Granulocyte colony-stimulating factor (hG-CSF) cDNA was linked to 3' untranslated region of hGH gene in the pGbc5.5hGH via EMCV IRES sequences. Transgenic mice were generated by microinjection and transgene expression was examined in the milk and mammary gland of transgenic mice at 10 days of lactation. Northern blot analysis showed that hGH gene and hG-CSF cDNA were transcribed as a single dicistronic mRNA. The hG-CSF and hGH proteins were independently translated from the dicistronic mRNA and secreted into the milk of transgenic mice. The highest concentration of hG-CSF and hGH in the milk of transgenic mice were and , respectively. In contrast, another hG-CSF expression cassette, in which hG-CSF genomic sequences were inserted into a commercial milk-specific expression vector (pBC1), generated a lower level () of hG-CSF expression in the milk of transgenic mice. These results demonstrated that the novel pGbc5.5hGH-based dicistronic construct could be useful for an efficient cDNA expression in the milk of transgenic animals.

This study aims to explore micrometeorological influences on particle growth by the experimental and statistical approaches. Characteristic meteorological conditions of the nucleation event were observed, and turbulence in the PBL seemed to play a role in particle growth. The major micrometeorological factors governing particle size growth in the PBL were instability (-z/L), friction velocity (u*), Bowen ration (B), and downward shortwave radiation (RsDN) as determined by a regression model. This result indicates that the geographical properties including land surface cover and geomorphology have strong influence on the aerosol size growth in PBL.

일본 학교교육에 있어서 국제이해교육이 처음으로 거론된 것은 1998년 개정 학습지도 요령 중의「종합적 학습시간」에서였다. 물론 국제사회에 대해서는「사회과」등을 통해 학습이 다루어져 왔으나, 이는 외국의 지리 사회 등을 이해하는 데 그쳤었다. 1986년 임시교육심의회의 제 2차 답신이 계기가 되어 국제 이해교육을 실시하였다. 답신의 내용은 학교교육에 대해 다음과 같이 3가지의 실현을 요청하는 것이었다. ① 폭넓은 시야에서 일본 사회 문화의 특성을 주장할 수 있으며, 다양한 이문화의 뛰어난 특성도 깊이 이해할 수 있는 능력을 지닌다. ② 일본인으로서 나라를 사랑하는 마음을 가짐과 동시에, 자국에 대한 이해만으로 상황을 판단하는 것이 아니라, 폭넓은 국제적, 지구적, 인류적 시야 속에서 인격의 형성을 지향한다. ③ 다양한 이문화를 깊이 이해하고, 충분히 의사소통이 가능한 국제적 커뮤니케이션 능력의 육성을 도모한다. 위 답신의 발표 배경을 토대로 본 논문에서는 일본 학교교육의 역사를 고찰하였다. 그리고 사회 변화에 호응하며 다음의 두 가지 사고가 경합하면서 일본의 학교교육이 이루어져 왔다는 사실을 분명히 하고자 하였다. ① 일본 사회 문화를 어떻게 전달할 것인가. ② 미래 사회를 짊어질 아이들에게 어떠한 능력이 필요한가. 또한 그를 어떻게 획득시킬 것인가. 일본사회가 다양화 국제화 되어가는 상황 속에서, 국제이해능력 육성은 차세대를 짊어질 아이들에게 있어 필수 불가결하다. 그러나 현 국제이해교육은 영어 능력 육성에 초점이 맞춰져 있는 경우가 많아 본래의 목적이 달성되고 있다고는 말하기 어렵다. 그러므로 금후 위의 답신을 실현하는 교육실천이 필요하며 이를 위해 국제적 시야를 지닌 교원 양성이 필요하다. 그 하나의 시도로서 나가사키대학과 한양대학교가 실시하고 있는 교육실습을 포함한 단기유학제도는 중요한 의미를 지닌다고 생각한다.

Three-year-old seedlings of susceptible Pinus densiflora and resistant Pinus x rigitaeda were each inoculated with the pinewood nematode, Bursaphelenchus xylophilus, to compare disease development. Needle dehydration was evident on seedlings of P. densiflora by 20 days after inoculation, 10 days earlier than this symptom was observed on P. × rigitaeda. Xylem drying was more frequent in seedlings of P. densiflora than in that of P. × rigitaeda between 20 and 60 days after inoculation. No significant differences were found between P. densiflora and P. × rigitaeda for stem water content or for stem and leaf relative water content in current-year branches after nematode inoculation, but the average number of B. xylophilus recovered from stems differed significantly between the two groups. The number of B. xylophilus recovered from stems was negatively correlated with the stem water content and with stem and leaf relative water content. By the time the experiment was terminated at 60 days after inoculation, all 3 of the last group of P. densiflora seedlings had died, but 2 of the 3 remaining P. × rigitaeda hybrid seedlings were still alive. Additional studies are needed to further explore the specific mechanisms preventing nematode multiplication in the seedlings of resistant P. × rigitaeda.

Eliot's image as a highly reputable and influential literary figure does not go along with the personality as found in Four Quartets in terms of analytical psychology. What the reader is led to find there is the return to the world of his childhood, where the poet is ruled by the mother, rather than to seek for equal and harmonious terms with the anima-figures. Eliot strongly advocates Ihe communion wilh the Logos, which is parallel 10 the psychological encounler with the Self, the God-image. However, in Four Quartets there is hardly found any feminine participation, which is a vilal preliminary step for the union of the ego with Ihe Self. The role of the feminine element, the anima, in the development of the psyche is to strengtheD ego-consciousDess against the overwhelming power of the unconscious. The ego-coDsciousness, firmly established wilh stability and autonomy, is then ready to experieDce the EDcounter without the risk of self-diss이utiOD， achieving a harmony and balance in its relalioDship to the Self. Four Quartets is dominated by the motif of self찌bnegatioD and Eliot here appears to deDy the value of autonomy of the human, urging giviDg it up ahogether for an access to the Logos. Analytical psychology would n이 see this posture as any way leading to the goal of psychological development, the individuatioD, but just a regression to the mother-ruled childhood.

고준위방사성폐기물처분을 위한 부지특성평가 기술을 구축하고, 이를 활용하여 심부지질환경을 이해하기 위해 1997년부터 지금까지 한국원자력연구원 주변 지역을 고준위폐기물처분을 위한 연구지역으로 선정하여 다양한 지질 관련 연구를 수행해왔다. 특히, 2002년에는 고준위폐기물의 처분 대상 심도의 시추공 (지하 500m)을 굴착하였으며, 2006년에는 지하처분연구시설 (KURT, KAERI Underground Research Tunnel)을 준공하여 연구지역에 대한 심부지질환경 규명을 위해 노력하고 있다. 현재, 지하처분연구터널의 좌측 연구용 모듈 내에 500 m 길이의 장심도 시추공 및 지하처분연구시설에서 남쪽으로 약 200 m 이격된 위치에서 1,000m의 장심도 시추공에서 다양한 부지특성평가 연구를 수행 중에 있다. 본 연구는 고준위폐기물의 심지층 처분을 위한 요소기술인 심부영역의 부지특성평가기술을 구축하기 위해 수행되었으며, 수리지질학적인 관점에서 부지특성평가의 기본 모델이 되는 3차원 지질모델을 구축한 내용이다. 연구지역에서 수행된 지표 지질조사와 시추공 자료를 이용하여 종합 분석한 결과, 수리지질학적 관점에서 중요한 풍화대, 상부 저경사단열대, 심부 영역에 존재하는 결정론적 단열대를 규명하여 3차원으로 모형화하였으며, 향후 본 연구를 통해 도출된 지질요소의 수리지질특성을 평가하여 고준위방사성폐기물처분 분야에 중요한 기술인 심부 영역의 수리지질환경을 이해하는데 활용될 예정이다.