남성 불임에 있어서 유전적 결함은 중요한 요인 중 하나이다. 정자 형성은 매우 복잡한 과정을 거치므로 생식세포의 완전한 발달을 조절하는데 많은 유전자들이 관여할 개연성이 높다. 이들 유전자 중에서 불임 유발 유전자는 다른 기능은 정상이되 생식에만 제한적으로 영향을 미치는 유전자들로 정의될 수 있다. 불임 남성에게서 Y 염색체의 미세결실(microdeletion)이 자주 관찰되었고, RBM과 DAZ라는 유전자가 그 위치에 존재함이 알려졌다. 또한 상 염색

본 연구에서는 보다 효율적인 동결 보존법을 수립하기 위하여 현재 사용되는 동결 보존액과 동결방법을 정자의 운동성 측면에서 비교해 보았다. 즉, 세 종류의 조성이 다른 동결보존액인 TYB, dithiothreitol을 첨가한 TYB+DTT, KS II 등이 동결보존 전후에 있어 운동성에 미치는 영향을 조사하였으며, 또한 vapor freezing 방법과 computerized freezer를 사용한 동결방법이 정자 운동성에 미치는 영향을 알아보았다. 정자

Recent studies have demonstrated that apoptotic cell death plays an important role in the mechanism underlying follicular atresia and luteolysis. However, the mechanisms responsible for initiating these processes have not been elucidated. In in vitro fertilization (IVF) programs, it is highly possible that continuous and repeated administration of FSH/hMG and GnRH agonists for the usage of ovarian hyperstimulation may induce apoptotic death of granulosa cells leading to atresia in the human ovarian follicles. The present study was performed to investigate whether FSH/hMG and GnRh agonists used for a longer period in controlled ovarian hyperstimulation has any effect on the apoptosis of granulosa-luteal (GL) cells obtained from hyperstimulated ovaries. To examine apoptotic cell death in the GL cells, cells were stained with acridie orange followed by observed in some of GL cells. Similar but distinct staining of apoptotic GL cells was observed when the cells were examined by using in situ TUNEL method. The healthy-looking cells with normal nuclear morphology were not stained, whereas cells with pyknotic nuclei or with apoptotic nuclei were intensively stained. After examining the ultrastructural features of GL cells by TEM, it was confirmed that the majority of cells seemed to have normal nuclei while GL cells undergoing apoptotic cel death were rarely found. The DNA extracted from GL cells showed a typical pattern of fragmentation following DNA electrophoretic analysis. We have confirmed that the apoptosis occurs in granulosa-luteal cells obtained from hyperstimulated ovaries. Technically, in situ apoptosis detection method is simple and reproducible and is well suited to identify the quality of oocytes retrieved from hyperstimulated ovaries.

The present study was conducted to determine the effect of recombinant human follicle stimulating hormone (rhFSH) on the estrogen synthesis by human fetal ovarian tissues. Fetal ovaries were 18-19 weeks old (18 wks:n=1, 19 wks:n=2). One case of 19-week-old fetal ovaries were obtained from anencephalic fetus. Obtained ovarieswere cleaned and diced around pieces, and cultured in the sandwich agar bed system for 21-23 days. Estrone () and estradiol-17 () in the medium was measured by radioimmunoassay. Amount of synthesis was greater than in both normal cases. In contrast, fetal ovaries from anencephalic fetus did not produce neither nor in the presence or absence of rhFSH. Results suggest that the fetal ovaries have a capacity of estrogen production at 18-19 weeks of gestation Existence of FSH receptor is also supposed. Different results by anecephalic fetal ovaries suggest the difference in the development between normal and anencephalic fetal ovaries.

The safety of the ship manoeuver is dependent not only on the inherent characteristics of the ship itself and environmental conditions, but also on the skill and experience of the ship operator. As the ship operator's judgement is related to his experience and skill, based on outside information, piloted controllability also depends upon the effectiveness, accuracy and reliability of various information which is obtainable from navigational equipments and aids to navigation that are available to the ship ope-rator. Although these factors, human factors and informational factors, have been pointed out as major factors which affect the controllability of a ship, there was no a comprehensive examination on the effect of these two factors. In this thesis, therefore, an attempt was made to examine whether or not there is clear indication that these two factors affect the controllability of a ship in reality. Experiments were conducted using the port design simulator of the Korea Maritime University.

The problem of supply and transport of sediment from a mountainous catchment is very important in explaining dynamic geomorphology and the hydrological cycle. The discharge of suspended sediment is determined by a morphological system. Human interference to environment is also an important, not negligible factor in sediment production. Moreover, growing concern in recent years for the problems of nonpoint pollution and for the transport of contaminants through terrestrial and aquatic ecosystem,; has highlighted the role of sediment-associated transport in fluvial systems. This study was conducted in forested and quarried catchments in order to clarify the different discharge process and the mechanism of suspended sediment dynamics for each catchment. As a forested catchment, the Yamaguchi River catchment which drains a 3.12 ㎢ area was chosen. On the other hand, the Futagami River basin, which is formed by three subbasins (1.07, 1.59 and 1.78 ㎢), as a quarried catchment was selected. These catchments are situated to the north and east of Mt. Tsukuba, Ibaraki, Japan. The discharge pattern of suspended sediment from the Futagami River basin is more unstable and irregular than that from forested catchment, the Yamaguchi River catchment. Under the similar rainstorm conditions, suspended sediment concentration from quarried catchment during a rainstorm event increases from 43 to 27,310㎎/l. However, in the case of the forested catchment it changes only from nearly zero to 274 ㎎/l. Generally, the supply source of suspended sediment is classified into two areas, the in-channel and non-channel source areas. As a result of field measurements, in the case of the forested catchment the in-channel (channel bed, channel bank and channel margin) is the main source area of suspended sediment. On the other hand, remarkable sediment source area on the quarried catchmen, is the non-channel that is unvegetated ground.

The transportations of most of cargoes in world trade have been fulfilled through sea lanes and it seems this trend will not change in near future. Nowadays, inview of the technical aspects of merchant vessels, they are continuously enlarged in hull size and greatly specialized in structure for the cargo spaces and dramatically automatized in navigating, piloting, cargo operating and various other operations, which unavoidably require high technicals in operating the modern merchant vessels. On the contrary to the trend of requiring of operating high technicals, the capabilities of crew on board have been gradually declining in technical competence and their morale for accomplishing their duties on board vessels has greatly falled won compared with that of old days. The above result inevitably brings many problems in operating modern vessels and causes accidents which are avoidable through good competence and high morale of the crew. We intend to analyze the causes of several great sea accident with which it seems some human errors are connected more or less directly or indirectly. We hope that this study could suggest some measures which help to prevent the recurrence of similar accidents by Korean ship's crews.

This paper deals with a mathematical model which explains process of aquisition or skillfulness of human. Especially, in the case of determinating a ship's position, a degree of aquisitiion or skillfulness has a significant meaning in the view point of reliability of a data because it is closely connected with the position error. In this paper, the authors have obtained the following mathematical model, who analyzed quantitavely the data obtained by a process of measuring the horizontal sextant angles and extracted the factors or parameters affecting the process of aquisition or skillfulness of human. Namely, H=Me-iN M : the coefficiency which represent the degree of difficulty in measuring the object i : personal parameter N : the number of measurements