The cryopreservation of Hanwoo embryos has become an integral part of assisted reproduction in animal. The objective of this study was to assess the effect of The objectives of this study were: (1) to evaluate the influence of bovine embryo developmental stage on in vitro embryo development after freezing, (2) to study the efficiency compared with conventional freezed embryos at different embryo source. For conventional slow-freezing, day 7 or 8 expanded blastocysts were collected. The standard freezing medium was 1.8 M ethylene glycol (EG). Embryos were equilibrated in 1.8 Methylene glycol(EG) with 0.1 M sucrose in Dulbecco's phosphate-buffered saline (D-PBS) supplemented with 0.5% bovine serum albumin. Embryos were then loaded individually into 0.25 ml-straw and placed directly into cooling chamber of programmable freezer precooled to , after 2 min, the straw was seeded, maintained at for 8 min, and then cooled to at /min, plunged and stored in liquid nitrogen for at least 3 days. For thawing, the straw containing embryos were warmed in air for 10 see and exposed to water for 20 sec. Straws were then removed from water. Rates of blastocyst survive and hatched were evaluated at 12 to 48h post-warming. The re-expansion and hatched rates of morula embryos were significantly lower than those obtained for blastocysts and expansion blastocysts (31.6%, 10.5% vs, 68.9%, 22.2% vs, 73.7%, 53.6%, respectively). No differences in re-expansion rates were found between in vivo and in vitro blastocysts. whereas hatched rates was significantly higher (51.2%) in vivo compared with in vitro embryos (18.6%). in conclusion, demonstrate that conventional freezing can be used successfully in cryopreservation of in vitro and in vivo bovine embryos, and that it might be considered for use in commercial programs and embryo preservation.

This study was carried out to investigate inflammation-related gene expression altered in ovary and endometrium of Korean cattle with reproductive disorders using microarray. In the present study, nine inflammation-related differential1y expressed genes (DEGs) were identified in the cystic ovary and endometrium with endometritis. In the follicular cyst, eotaxin and alpha-2-HS-glycoprotein (AHSG) were up-regulated, whereas complement component 3 (C3) and oxidised low density lipoprotein (lectin-like) receptor 1 (OLR1) were down-regulated. Complement component 4A (C4A) was up-regulated in luteal cyst. In the endometritis, chemokine 1igand l and 2 (CXCL1 and CXCL2), protein C (inactivator of coagulation factors Va and VIIIa), and complement component C5 were up-regulated, whereas kininogen was down-regulated. Of these genes, we focused on eotaxin and kininogen, which were highly regulated in the follicular cyst and endometritis, respectively and on C3 commonly regulated in both reproductive disorders. The microarray data of eotaxin, kininogen, and C3 were validated by semi-quantitative PCR. Consistent with microarray data, eotaxin was up-regulated by 4-fold in the follicular cyst, while kininogen was down-regulated by 5-fold in the endometritis. C3 was down-regulated in the both follicular cyst and endometritis. Our results suggest that these inflammation-related genes could be useful markers for diagnosis of cystic ovary and endometritis of Korean cattle.

The objective of this study is to investigate the changes in concentrations of leptin and insulin in serum of Korean cattle (Hanwoo) with reproductive disorders and to examine the relationship among leptin, insulin, and body condition score (BCS). The concentration of leptin in serum of pregnant Hanwoo showed insignificant difference from that in serum of Hanwoo with reproductive disorder, such as repeat breeding, follicular cyst, corpus luteum cyst, ovarian atrophy, and feeble estrus (p>0.05). However, the concentrations of leptin and insulin in serum were changed with different BCS value. In emaciated Hanwoo (BCS ), they were significantly decreased compared to BCS (p<0.05). The leptin showed different genotypes with different BCS value. In BCS , C/T genotype was expressed (83.3%) more than C/C (16.7%) or T/T (0%) genotype, whereas C/C genotype was expressed (62.5%) more than C/T (25.0%) or T/T (12.5%) genotype in BCS . The insulin concentration in follicular fluid obtained from ovary with follicular cyst which has follicles having diameter of was significantly higher (p<0.05) than those in normal follicle fluid which has follicles having diameter of . These results showed that concentration of leptin and insulin in serum were related to BCS value and follicular size and suggest that the changes in concentration of leptin and/or insulin in serum could be a potent biomarker for diagnosis of bovine reproductive disorder.

PPS(Post-tensioned Precast concrete System)공법은 U자형 PC로 제작된 넓은 보와 PC또는 현장 타설 콘크리트로 제작한 기둥으로 구성되며, PC보와 기둥의 일체성 확보를 위하여 프리스트레스를 도입하였다. 본 연구에서는 포스트텐션을 도입한 넓은 보-기둥 접합부의 구조적 특성을 규명하고, 다양한 변수해석이 가능하기 위한 자료를 제공하고자 유한요소해석 프로그램인 ANSYS을 사용하여 비선형 해석을 수행하였다 콘크리트에 대한 해석요소는 8개의 절점을 가지며 각 절점이 3개의 자유도(X, Y, Z축에 대한 병진 변위)를 갖는 Solid 65요소를 사용하였다. Solid 65요소에서 전단전달계수(Shear-Transfer factor)는 실험값에 근사적으로 해석값을 맞추기 위한 영향 계수값으로 균열이 발생하는 위치에 대한 전단강도의 감소를 반영한다. 그 결과, 본 실험체에 대한 해석에서는 열려진 전단전달계수 0.125와 닫혀진 전달계수 0.85에 기초하여 해석한 결과 닫혀진 전단전달계수는 0.85에서 열려진 전단전달계수에서는 0.2일때 가장 실험값에 근사한 해석치를 보였다.

The objective of this study is to investigate the changes in concentrations of blood urea nitrogen (BUN) and sex steroid hormones, such as estrogen, progesterone, and testosterone in Korean cattle (Hanwoo) with reproductive disorders and to examine the relationship between BUN and body condition score (BCS) in Hanwoo. The concentration of BUN was 16.2 mg/dl, 17.8 mg/dl, 15.1 mg/dl, 17.9 mg/dl, and 28.3mg/dl in pregnancy, repeat breeding, follicular cyst, luteal cyst, and ovarian atrophy, respectively. In Hanwoo with BCS , and , the concentration of BUN was 15.8 mg/dl, 17.0 mg/dl, and 17.6 mg/dl, respectively. Fluoroimmunoassay showed that serum estrogen and progesterone levels were decreased in reproductive disorders Hanwoo, such as ovarian atrophy, endometritis, and weak estrus. The testosterone level was significantly decreased in Hanwoo with reproductive disorders compared to that in pregnant Hanwoo ( vs 0.13 ng/ml, p<0.05). The progesterone and estrogen concentrations in follicular fluid obtained from ovary with follicular cyst were significantly higher (p<0.05) than those in normal follicle fluid. These results show that there is no relationship between BUN and BCS in Hanwoo, and the concentration of sex steroid hormone in serum and follicular fluid are changed in reproductive disorders Hanwoo.

비선형 구조계의 확률론적 지진해석 방법 중 대표적인 것은 지진 재해도 수준에 해당하는 입력지반운동 모델을 사용한 시간이력을 수행하여 그 응답의 확률분포를 예측하는 것이다. 이 연구에서는 널리 사용되고 있는 두 가지 입력지반운동 모델에 따른 구조계 응답의 분포특성 및 파손확률의 차이와 그 원인을 분석한다 입력지반운동 모델로는 실제 지진기록을 배율 조정하여 사용하는 배율조정 입력지반운동과 설계 응답스펙트럼에 상응하는 인공 지진기록을 사용하는 스펙트럼 맞춤 입력지반운동 두 가지를 고려한다. 동일한 지진재해도 수준을 고려한 해석결과 설계 응답스펙트럼에 상응하는 인공 지진기록을 사용한 입력지반운동 모델은 실제 지진기록을 배율 조정한 입력지반운동 모델보다 평균적으로 응답을 크게 평가하였고 이로 인해 파손확률 또한 더 큰 것으로 나타났다 이러한 경향은 연약한 지반에서 더욱 현저한 것으로 나타났다. 이러한 입력지반운동 모델에 따른 파손확률의 차이는 스펙트럼 맞춤 입력지반운동의 목표로 사용된 도로교 설계기준의 설계 응답스펙트럼이 실제 지진기록의 응답스펙트럼보다 장주기로 갈수록 응답을 크게 평가하도록 보수적으로 만들어졌기 때문인 것으로 나타났다.