본 연구는 소에 있어서 다량의 수정란을 생산하여, 고능력의 유전인자를 조기에 확산할 수 있도록, 한우 공란우의 반복 과배란을 유도하여 안정적이고 효율적인 수정란 생산을 목적으로 실시하였으며, 그 결과는 다음과 같다. 1. 한우 경산우 및 미경산우를 4회 반복적으로 과배란 처리하였을 때, 수정란 채란이 가능한 공란우는 였으며, 3회 처리시 다소 낮아, 약간의 유의차가 인정되었다(P<0.05). 2. 한우 경산우 및 미경산우를 4회 반복적으로 과배란 처리하

본 연구는 정상 및 저수태 한우에 rbST를 투여하였을 때 수태 및 분만율에 미치는 영향을 조사하기 위하여 수행하였다. 시험축은 성빈 한우 383두와 임상적으로는 정상이나 3회 이상 수정시켜도 수태가 되지 않는 저수태 한우 79두, 총 462두를 시험에 공시하였다. 실험을 수행하기 위한 시험구 배치는 5개의 처리구, 즉 T1(무처리, 2ml의 생리 식염수), T2(rbST 250mg 수정시 1회 미근부에 투여), T3(rbST 250mg: 수정시 및 수

수정란의 성 판별은 유전적으로 우수한 유전형질을 보유하고 있는 소의 수정란을 성 판별하므로서 희망하는 성의 송아지를 생산할 수 있으며, 부가가치가 높은 수정란을 확보할 수 있는 기술이다. 수정란의 손상을 최소화하면서 할구를 biopsy하는 기술을 개발하고, 간단하고 빠른 시간에 성 판별이 가능한 Loop-mediated isothermal amplification방법으로 수정란을 성 판별을 실시한 결과는 다음과 같다. 1. 한우 체내 수정란의 성비는 암

혈통이 등록된 우수한 한우 공란우로부터 회수한 수정란을 간편한 방법으로 성 판별하여 우수한 암송아지를 생산하고자 실시한 결과는 다음과 같다. 회수한 수정란을 punching 또는 bisection 방법으로 biopsy하여 Loop-mediated isothermal amplification법으로 성 판별하였으며, 암컷으로 예측되는 성 판별 수정란을 6두의 수란우에 이식하여 2두가 임신되었고, 수정란 이식 후 278일과 285일에 정상적인 암컷 송아지를

These obscrvations were performed to investigate the safety of the natural herbs (Rhus-lI) in respect of genotoxicity. This substance was examined in two in-vitro tests : (1) Salmonella typhinurium reversion assay (Ames test) in strain TA 98, TA 100, TA 1535 and TA 1537, (2) in vitro chromosome aberration test in cultured Chinese hamster ovary (CHO) cells. In the reverse mutation test, Rhus-II did not induced mutagenicity in Salmonella typhimurium reversion assay(Ames test) with or without metabolic activation. In the chromosome aberration assay using CHO cells. there was no increased incidence of structural and numerical aberrations with or without metabolic activation. `these results indicated that, the Rhus-II had no genotoxicity.

The purpose of the present study was to investigate differences in the sensitizing potential of Rhus Veniciflua(Rhus-II), when tested by the guinea pig maximization test(GPMT) and Freund's complete adjuvant test(FCAT) with an identical, intradermal induction concentration. A new grading. classification of the sensitization potential is proposed. The GPMT was conducted according to OECD guideline #406, using a multiple-dose design and test results were analysed with logistic regression analysis. During the induction stage, we injected intradermally each three site 0.1 ml( 1mg/animal) test material. 0.1ml complete Freund's adjuvant and 0.1ml the test agent emulsified in the adjuvant. 7 days later, we induced weak sensitization with 10% sodium lauryl sulfate(SLS) and applide 1ml(10mg/animal) test agent topically on the same site and made a tight occlusion. 14 days later we challenged with 1ml(10mg/animal) of test material on the flank and observed ant 24 hours and 48 hours later. The results were also observed 0% at 24 hours challenge. The results observed 48 hours after challenge were the identical. These data indicated that. although Rhus-II is a no contact allergen. It was reported that the skin sensitization by Rhus-II was not detected the skin sensitization in the guinea pig maximization test (GPMT). Consequently, it was confirmed that Rhus-Il had no contact allergic sensitization in guinea pig maximization test.

The purpose of this study was to examine the four week repeated toxicity in Sprague-Dawley rats orally administrated with Rhus-II (water fraction of Rhus Veniciflua). In acute toxicity test, three groups (40 rats of both sex) were administrated different dosages of Rhus-II, 2 g/kg (high dosage group), 1 g/kg, 0.5 g/kg and one group (10 rats of both sex) were received by orally only saline according to the Regulation on Korea Food and Drug Administration, respectively. There was no difference in body weight change, feed intake and water consumption among different dose groups. There was no alteration in relative organ weight by the administration of Rhus-Il. No death of abnormal clinical signs was observed during the experimental period. Between the groups orally administered Rhus-II and the control group. there was no statistical significance in urinalysis, hematological test or serum biochemical values. There were no gross findings at final sacrifice. There was no evidence of histopathological alteration mediated by four week treatment with Rhus-ll. These results suggest that no observable effect level(NOEL) of the test orally administration was considered to be more than 2g/kg in rats under the conditions employed in this study.

This study was performed to investigate the effect of Lycii fructus beer on serum lipid profiles and antioxidant activity in rat Sprague-Dawley (SD) rats weighting about 190g were divided into the following 5 groups ; distillate water (Control), 5% ethanol in distillate water (Ethanol), commercial beer (CB), Lycii fructus beer (LFB) and 5% alcohol red wine diluted with distillate water (RW). Body weight, total food intake, FER and percent organ (liver, kidney) weight per body weight were not significantly changed by Lycii fructus beer drinking. After 6 weeks, serum total cholesterol, triglyceride and HDL cholesterol level were not significantly different. But, Lycii fructus beer intake tended to decrease serum triglyceride level and atherogenic index. Also, GOT and GPT levels were expressed lower than Ethanol group. There was not significantly different in hepatic glutatiione (GSH) content and glutathione-S-transferase (GST) activities among 5 groups. Lipid peroxidation in the hepatic was decreased by Lycii fructus beer intake. The results demonstrated that Lycii fructus beer was potential and effective antioxidant that can protect the decrease associated with alcohol.