We report the structural, morphological and magnetic properties of the Ni70Mn30 alloy prepared by Planetary Ball Mill method. Keeping the milling time constant for 30 h, the effect of different ball milling speeds on the synthesis and magnetic properties of the samples was thoroughly investigated. A remarkable variation in the morphology and average particle size was observed with the increase in milling speed. For the samples ball milled at 200 and 300 rpm, the average particle size and hence magnetization were decreased due to the increased lattice strain, distortion and surface effects which became prominent due to the increase in the thickness of the outer magnetically dead layer. For the samples ball milled at 400, 500 and 600 rpm however, the average particle size and hence magnetization were increased. This increased magnetization was attributed to the reduced surface area to volume ratio that ultimately led to the enhanced ferromagnetic interactions. The maximum saturation magnetization (75 emu/g at 1 T applied field) observed for the sample ball milled at 600 rpm and the low value of coercivity makes this material useful as soft magnetic material.

Molecular diagnostic markers are necessary for establishing highthroughput screening systems to support insecticide-resistant population management. Here, we identified single amino acid substitution mutations related to carbamate resistance in Laodelphax striatellus Fallén type-1 acetylcholinesterase (Lsace1) using carbofuran-selected strains. The phenotypic resistance profiles of the final selection strain (SEL9) compared to the susceptible strain revealed a 14-fold higher resistance ratio based on topical application, 1.2-fold higher general esterase activity, and 4.3- fold higher acetylcholinesterase insensitivity based on the 50% inhibitory concentration (I50), suggesting that insensitivity of the target site could occur as a resistance factor. Comparison of the nucleotide sequences of Lsace1 of five strains (SUS, SEL0, SEL3, SEL6, and SEL9) revealed two amino acid substitutions (F330Y and F331H). To understand the roles of these mutations, we determined the allele frequency of both point mutations in the selected strains using quantitative sequencing methods. In addition, several quantitative genotypic traits (e.g., gene copy numbers and transcript levels of Lsace1, Lsace2, and LS.CarE1) were assessed. A correlation analysis of genotypic and phenotypic traits revealed strong correlations between resistance level and I50 with F331H allele frequency. Interestingly, the F331H mutation was negatively correlated with transcript levels of Lsace1, suggesting that selection pressure might result in a reduction of the target gene. Overall, the F331H mutation and reduced mRNA are important factors in the development of carbamate resistance. Furthermore, the point mutation can be used to monitor rapid carbofuran resistance in conjunction with molecular diagnostic methods such as quantitative sequencing.

국내에서 재배하는 주요 핵과류(복숭아, 자두, 매실, 체리)에 발생하는 깍지벌레는 뽕나무깍지벌레(Pseudaulacaspis pentagona), 말채나무공깍지벌레(Lecanium corni), 무화과깍지벌레(Coccus hesperidum), 가루깍지벌레류(Pseudococcus sp.) 4종이 발견되었으나, 뽕나무깍지 벌레는 2017년에 총 113과원 중 103과원(발생과원율 91.2%), 2018년에는 77과원 중 64과원(발생과원율 83.1%) 모든 핵과류에서 가장 많이 발생하여 우점종으로 확인되었다. 2017년도에는 5월 상순부터 부화를 시작하여 암컷성충 1마리가 평균 75.5개(47∼159개)의 알을 낳으며 모든 알이 부화하는데 약 19일이 소요되었으며 5월 20일경에 월동성충의 모든 알이 부화하였다. 그러나, 2018년에는 4월 하순부터 부화를 시작하여 (부화율 72%) 부화유충(1령)으로 이동 후 5월 중순부터 고착유충(2~3령), 6월 중순부터 2세대 성충이 활동하기 시작하였다. 부화유충기(1령)에 약제 살포를 하면 살충율이 100.0%이었으나, 고착유충기(2~3령)에는 살충율은 2.7%에 불과하였다. 부화약충기를 제외한 모든 단계에서 몸체가 밀납깍지로 덮여 있어 약제를 살포하여도 직접 접촉되지 않아 치사율이 낮았다. 따라서 반드시 부화약충기에 약제를 살포하여야 방제효과를 높일 수 있다.

ICT 기반의 해충 이미지 전송 시스템은 담배거세미나방과 파밤나방을 예찰하고 방제하는데 필요한 효과적인 유인 해충 전송수단이다. 시설 온실내 담배거세미나방과 파밤나방을 유인하기 위하여 스마트 트랩을 이용하였다. 스마트트랩은 나방류 해충유인을 위해 성페로몬 4종 장착하였으며 유인된 해충은 특수 카메라를 이용하여 이미지 촬영이 가능하였다. 또한, 해충유인 발광트랩 처리구는 황색광을 사용한 발광 트랩을 사용하였다. 또한 대조구로 기존에 널리 사용되는 델타트랩은 한 종류의 성페로몬을 장착하여 유인력 검정에 이용하였다. 성페로몬 한 종류만 사용한 델타트랩 처리는 담배거세미나방 페로몬 루어를 사용하였고, 4종류의 성페로몬을 설치한 스마트 트랩 처리구는 담배거세미나방, 뒷날개흰나방, 파밤나방, 왕담배나방용 성페로몬 루어를 사용하였다. 실험은 2018년 6월 1일부터 8월 31일까지 함안 시설원예연구소의 13m2의 소형비닐하우스에서 수행하였으며 공시작물은 파프리카 ‘시로코’ 품종과 토마토 ‘데프니스’ 품종을 사용하였다. 나방류 해충의 유인력 검정결과 파프리카와 토마토를 식재한 온실 모두 성페로몬 루어 한 종류를 설치한 델타트랩보다 유인발광 트랩 처리구에서 높은 나방류 유인율을 나타내었다. 시험기간 유인발광 트랩의 경우 유인 포획된 총 나방수는 파프리카 온실에서 평균 97마리, 토마토 온실에서 75마리가 유인 포획되어 가장 높았다. 그러나, 성페로몬 1종 및 4종을 설치한 델타트랩 및 스마트트랩처리구는 두 개의 온실 모두 평균 5마리 이하의 유인율을 나타내었다. 따라서, 시설내 나방류 예찰 및 유인 방제하기 위해서는 기존 성페로몬만 사용하기 보다는 유인발광 트랩을 함께 설치하는 것이 나방류 유인포획에 효과적이라고 판단되었다.

친환경 해충 방제 방법 중 LED를 비롯한 빛을 이용한 해충방제가 활발하게 연구되고 있으며, 해양, 농장, 축사 등 산업적인 측면뿐만 아니라 사회 전반적인 분야에서 활용되고 있다. 해충 기피램프의 광원은 주로 LED(550~630mm)가 활용되고 있으나 출력이 약해 넓은 면적에 적용이 힘든 단점이 있다. 이에 제논램프를 이용한 넓은 면적에 적용 가능한 식물재배용 해충 기피램프를 적용하여 담배거세미나방에 의한 작물 및 피해과실률을 비교하였다. 특정 파장(870~920mm, 자외선:가시광선:적외선=2:34:64)을 방출하는 제논램프를 높이 2.5m, 2/10a개 설치하였다. 광원의 처리는 3수준(일몰 후 2시간 경과 시점부터 2시간, 4시간, 무처리)으로 하였으며, 나방의 유입을 원활하게 하기 위하여 모든 출입구를 개방하였다. 과실 피해가 발생한 후 출입구를 폐쇄하여 제논램프가 유입된 나방의 작물 및 과실 가해를 억제하는지 조사하였다. 토마토 재배는 경상남도 함안(35° 15’ 0”N / 128° 25’ 0”E)의 유리온실(6*10*5m)에 토마토(데프니스, 유럽종)를 재식간격 1m(줄 간격), 0.5m(포기사이거리)재배하였다. 생육기간은 4월 12일 정식하여 8월 2일까지이다. 토마토 재배기간 동안 격주간격으로 생육(초장, 엽면적, 잎, 과실, 줄기의 생체중 및 건물중)조사를 하였으며, 매주 피해과, 정상과의 수량을 조사하였다. 조사 결과 대조구에서 엽의 건조 중량이 21%정도 낮았으며, 줄기의 건조 중량은 2.5배 높았으며, 초장, 엽면적은 차이가 없었다. 제논램프 처리 시간에 따라 피해과실률((피해과실 중량(g)/총 과실 중량(g))*100)은 제논램프 4시간 : 초기 7.59 → 2주 후 0.02a, 2시간 : 초기 3.41 → 2주 후 6.32b, 대조구 초기 10.00 → 2주 후 42.71c% (Analysis of variance, p < 0.1) 로 조사되었다. 대조구에서는 유입된 담배거세미나방이 온실 내에서 번식함에 따라 엽의 생체중은 감소하였으나 엽면적에서는 유의한 차이를 보이지 않았는데, 이는 대조구의 잎이 심하게 황화된 경우가 많았고, 이 때문에 엽면적은 증가하였으나 중량감소 하였다. 제논램프 2시간, 4시간 처리에서는 담배거세 미나방에 의한 작물 및 피해과실률이 감소하였으며, 제논램프 4시간 처리에서 유의하게 높은 방제 효과를 보였다.

The purpose of the present study was to evaluate if main domestic vector mosquitoes, Aedes albopictus as a diurnal species and Culex pipiens as a nocturnal species, have color preference for blood feeding activity. Laboratory evaluations were performed and compared to assess color attractiveness using seven fundamental colors including black, white, red, yellow, green, blue, and purple. The test bodies selected for these experiments were BioGents Sentinel traps (BG traps) with 20g dry ice in each falcon tube, which covered outside of the traps attached with each different color of vinyl sheets. In each experiment, a pair of BG traps was placed 0.6 m away from each other in a 1.8 m x 3.7 m x 1.8 m screened enclosure. The enclosure maintained at 26±2℃, 65±5% RH, and 100 lux illumination. Evaluations were performed for two hours between 10:00 and 16:00 for each 25 Ae. ablopictus females and 15 hours from 18:00 to 9:00 for each 25 Cx. pipiens females. The attraction rates were determined by a series of 42 simultaneous pairs of sample counts of the number of mosquitoes caught in the traps at every 30 minutes. As a result, black traps collected the largest number of Ae. albopictus as a mean 0.7 of 1.0 for Mosquito Preference Index (MPI), followed closely by red, blue, and purple (MPIs, 0.6) that were significantly higher (p<0.05) than those of green (MPI, 0.5), white (MPI, 0.3), and yellow (MPI, 0.2). Similarly, the MPIs for Cx. pipiens were significantly higher at black and red traps (MPIs, 0.7) but the MPIs (0.3) at white and yellow traps were significantly lower than those of the other colors.

The purpose of the present study was to evaluate if main domestic vector mosquitoes, Aedes albopictus as a diurnal species and Culex pipiens as a nocturnal species, have correlation between reflecting luminous intensity of colors and preference for blood feeding activity. Laboratory evaluations were performed and the mosquito preferences were compared to three different color brightness such as light, primary, and dark for five fundamental color series including red, yellow, green, blue, and purple. The test bodies selected for these experiments were BioGents Sentinel traps (BG traps) with 20g dry ice in each falcon tube, which covered outside of the traps attached with three different luminous intensities such as light, primary, and dark for each different color of paper sheets. In each experiment, a pair of BG traps was placed 0.6 m away from each other in a 1.8 m x 3.7 m x 1.8 m screened enclosure. The enclosure maintained at 26±2℃, 65±5% RH, and 100 lux illumination. Evaluations were performed for two hours between 10:00 and 16:00 for each 25 Ae. ablopictus females and 15 hours from 18:00 to 9:00 for each 25 Cx. pipiens females. Each experiment was replicated four times. As a result, reflecting luminous intensity (L-value) affected and correlated negatively to the preference of the two mosquito species but the fundamental colors, green-red (a-value) and blue-yellow (b-value) were not correlated significantly to the preference (p=0.05). Aedes ablopictus females were preferred for feeding activity more to lower L-values of the colors and correlated negatively between color preference of the mosquitoes and the L-values such as r = -0.584 (p=0.003) for red, r = -0.606 (p=0.002) for yellow, r = -0.620 (p=0.001) for green, and r = -0.463 (p=0.023) for blue except purple (r = -0.129; p=0.549). Similarly, Cx. pipiens females were correlated negatively such as r = -0.590 (p=0.002) for red, r = -0.564 (p=0.005) for yellow, r = -0.687 (p=0.000) for green, r = -0.623 (p=0.001) for blue, and r = -0.689 (p=0.000). In conclusion, the color brightness were negatively correlated to the feeding activity of the two vector mosquito species but not the wavelength of visible ray. Also, the mosquito females preferred significantly darker ones in the same color series (p<0.05) except purple for Ae. ablopictus females.

Soybean cyst nematode, Heterodera glycines is one of the major plant parasitic nematodes in soybean. Recently, another soybean cyst nematode, H. sojae was recorded from Korea. This study was conducted to determine the geographical distribution of the two soybean cyst nematodes in Gangwon-do and Chungcheongbuk-do, Korea. In Gangwon-do, H. glycines and H. sojae were detected in 12 and 5 of the 53 sites sampled, respectively, while in Chungcheongbuk-do, H. glycines was isolated from 14 of the 46 sites sampled. H. sojae was detected at only one site. Additionally, the two soybean cyst nematodes co-existed in 3 of the 46 sites.

Clover cyst nematode, Heterodera trifolii have recently been identified as a serious pest to Chinese cabbage in highland area of Korea. This study was conducted to investigate the host specificity effect of H. trifolii by their inoculation density and temperature on Chinese cabbage and kale in pot in laboratory condition. H. trifolii inoculated with (1, 2, 4, 8, 16 eggs /g soil), where the number of female resulted higher in Chinese cabbage than in kale but there was no statistical differences between them. The number of cysts did not show any variation by their inoculation density in both plants. The results depicted the proliferation of H. trifolii by temperature (15, 20, 25, 30℃), where the number of females, cysts and eggs has their highest proliferation in 20℃ condition. The study thus demonstrated that, Chinese cabbage is considered a suitable host of the clover cyst nematode compared to the kale and the suitable temperature of the reproduction is estimated as 20°C.

Bemisia tabaci biotype Q in China (Shandong) orginated from the western Mediterrenean regions. B. tabaci biotype Q was introduced into Shandong province of China in 2006 and has since displaced the previous well-established population of biotype B in the whole province. The populations of B. tabaci biotype Q in the field have a rapid genetic turnover. These findings will be helpful to the management of whitefly populations in the field.

벼메뚜기는 분류학적으로 메뚜기목(Orthoptera), 메뚜기과(Acrididae)에 속하는 곤충으로 작물에 피해를 주는 해충으로 인식하고 있지만 예로부터 단백질 보충을 위하여 채집하여 튀기거나 볶음요리 로 이용해 왔다. 벼메뚜기는 갈색거저리, 쌍별귀뚜라미 등과 함께 식품공전에 등록되어 있으나 1년 1세대로 가을철에 채집하여 이용하는 실정으로 공급확대에 한계가 있다. 최근 우리나라에서 벼메뚜기를 사육하고자 하는 농가와 다양한 용도로 가공을 통해 이용하려는 수요가 점차 증가하고 있으나 연중사육기술이 개발되지 않아 대량사육하는 농가가 거의 없는 실정이다. 이에 벼메뚜기의 연중대량사육기술 뿐만 아니라 인공사료 및 자동화 사육시설 개발에 따른 생산비 절감 기술 개발이 필요한 실정이다. 벼메뚜기의 먹이로는 여름작물로 옥수수, 수단그래스, 겨울작물로 밀, 보리 등의 볏과작물이 있으며 많은 양의 생엽과 발육에 적합한 작물의 선발 및 볏과작물의 통곡실 가루를 이용한 인공사료의 개발로 먹이공급 부족 시 15~20일 급여 가능하며, 벼메뚜기 사육시설로 단독형, 연결형, 절충형 등의 사육시설을 개발하여 연결형 사육시설의 수확량과 생존율이 가장 높았으며, 예취 급이 노동력이 단독형의 16% 소요되었다.

To identify the venom components and their expression patterns of some Aculeata bees/wasps, venom gland-specific transcriptome analysis was conducted. FPKM values were normalized with the average of the transcription level of reference gene (a-tubulin). Common components in both solitary and social wasp venoms include hyaluronidase, phospholipase A2, metalloendopeptidase, etc. Although it has been expected that more diverse bioactive components with the functions of prey inactivation and physiology manipulation are present in solitary wasps, the information on venom compositions of solitary wasps obtained in this study was not sufficient to generalizae this notion. Nevertheless, some neurotoxic peptides (e.g., pompilidotoxin and dendrotoxin-like peptide) and proteins (e.g., insuline-like peptide binding protein) appear to be specific to solitary wasp venom. In contrast, several proteins, such as venom allergen 5 protein, venom acid phosphatase, and various phospholipases, appear to be relatively more abundant in social wasp venom. In the venom gland trancsriptome of bumblebees, major allergens or pain producing factors were barely identified, implying that bumblebee venoms are relatively less toxic than those of social or solitary wasps.

Laodelphax striatellus is an important pest of rice due to not only sucking rice seedlings, but also transmitting serious plant viruses. Among various kinds of insecticide groups (carbamates, organophosphorus, neonicotinoids, etc.), carbofuran, a systemic carbamate insecticide, has been most extensively used to control rice pests including L. striatellus, resulting in widespread carbamate resistance in Korea and other Northeast Asia countries. To identify the genes associated with carbofuran resistance, we obtained a 14-fold higher resistant strain (SEL9) from the mixed-field population (SEL0) by consecutive selection. A transcriptome-based analysis was conducted and differentially expressed genes (DEG) were compared between the SEL9 and SEL0 strains. A total of 96,185,150 reads were analyzed, of which 62,860,430 reads were mapped. From these reads, 15,356 transcripts were annotated. A total of 327 up-regulated and 275 down-regulated genes were identified in the resistant SEL9 strain compared to SEL0 strain by DEG analysis. Gene ontology (GO) analysis was performed using DEGs showing statistical significance (P < 0.05). The GO analysis identified 1,320 genes in biological process group, 1,100 genes in cellular component group and 428 genes in molecular_function group.

Pear psyllids belong to the most serious pests of pear. They damage pear trees by excessive removal of phloem sap, by soiling the fruits with honeydew which, in turn, provides a substrate for sooty mold, and by transmission of Candidatus Phytoplasma, the causal agents of the pear decline disease. The morphological similarity, the presence of seasonal dimorphism that affect adult colour, size and wing characters, and uncritical use of species names, led much confusion in the taxonomy of pear psyllid species. As a result, pear psyllids have been frequently misidentified. Here we analysed DNA barcodes of eleven pear psyllid species from eastern Asia, Europe and Iran using four mitochondrial gene fragments. The efficiency of identification was notably high and considerable barcoding gaps were observed in all markers. Our results confirm the synonymies of the seasonal forms. Previous misidentifications are also corrected. There is no evidence for the presence of European pear psyllid species in East Asia.

Geminiviruses are plant-infecting viruses with monopartite or bipartite single-stranded circular DNA genomes. They are known to be mediated by insects such as whiteflies, treehoppers, leafhoppers or aphids and can cause devastating plant diseases in a wide range of economically significant crops worldwide. In Korea, occurrence of geminiviruses were reported officially after the 2000s. Although Honeysuckle yellow vein virus (HYVV) and Sweet potato leaf curl virus (SPLCV) were identified from honeysuckle and sweet potato in 2004 and 2006 respectively, these viruses did not spread and cause much concern for geminiviruses. In 2008, Tomato yellow leaf curl virus (TYLCV) that has caused severe tomato production loss in many subtropical and tropical countries was first reported in tomato plants cultivated in Tongyeong. TYLCV rapidly spread through the country and has been continuously reported from tomato cultivating areas in Korea. In addition to TYLCV, Tobacco leaf curl virus and Sweet potato golden vein associated virus have occurred. In recent years, new geminiviruses including Papaya leaf curl Guangdong virus (PaLCGdV) and Euphorbia leaf curl virus (EuLCV) have been introduced. Newly emerging geminiviruses from tropical and subtropical countries are due to increased demand for various fruits and vegetables, and climate change. In addition, there are reports that some geminiviruses including TYLCV can be transmitted by infected seeds. Therefore it is important to study on epidemiology of virus introduction and spread among the countries and within country.

Samples were collected from planktonic habitats of the fresh and brackish waters in Korea from August 2016 to May 2018. As a result, three genera and nine species were newly recorded in Korea. The unrecorded indigenous genera were Anathece, Chondrocystis and Geminocystis, and nine species were Anabaenopsis arnoldii, Anathece smithii, Chondrocystis dermochroa, Coelosphaerium aerugineum, Eucapsis microscopica, Geminocystis herdmanii, Microcystis panniformis, Synechococcus nidulans and Woronichinia karelica. Anathece smithii, Coelosphaerium aerugineum, Eucapsis microscopica, Microcystis panniformis and Synechococcus nidulans had been reported to inhabit freshwater, but these were found in brackish water in this study. Microcystis panniformis, which is a potential genus for causing green-tide, is taxonomically valuable in Korea.

Phytoplanktons were collected from various environments including small marshes, small ponds, reservoirs and brackish water from March 2017 to November 2017. In this study, five species of family Scenedesmaceae were newly recorded in Korea. The genus Desmodesmus was newly reported in Korea. The newly recorded species are Scenedesmus nanus, S. praetervisus, Desmodesmus costato-granulatus, D. lunatus and D. spinulatus. S. nanus and S. praetervisus are known to occur in freshwater, but they were found in the brackish water in this study.

The present study was conducted to evaluate the effects of high levels of nutrients on the growth performance, blood metabolites and carcass characteristics of Hanwoo cattle. Eighteen Hanwoo steers were fed two types of diets: 1) Nine animals were fed the conventional diet including typical levels of crude protein (CP) and total digestive nutrients (TDN), and 2) Nine animals were fed the treatment diet including high levels of CP and TDN. The average body weight (BW) and dry matter intake (DMI) were greater (P < 0.05) in the treatment group than in the conventional group at early and late fattening stages. Also, in the treatment group, the average daily gain (ADG) was greater (P < 0.05) at the late fattening stage. The serum total lipid and cholesterol levels were higher (P < 0.05) in the late fattening stage of treatment group. The carcass weight, total fat weight, longissimus muscle area and the grade of meat quality were also greater (P < 0.05) in the treatment group than the conventional diet group. This study demonstrates that high levels of CP and TDN exhibit a positive effect on the growth performance and carcass characteristics, indicating that high levels of CP and TDN can be used as a cost-effective feeding program for Hanwoo cattle by shortening the feeding period.

Pesticide application in agriculture provides significant benefits such as protection from disease, prevention of harmful insects, and increased crop yields. However, accurate toxicological tests and risk assessments are necessary because of many related adverse effects associated with pesticide use. In this review, we discuss and analyze residual pesticides contained in livestock feed in Korea. A pesticide residue tolerance standard for livestock feed has not been precisely established; so, risk assessments are required to ensure safety. Standards and approaches for animal criteria and appropriate methods for evaluating residual pesticides are discussed and analyzed based on technology related to animal product safety in Korea. The safety of livestock feed containing pesticides is assessed to establish maximum residue limits relative to pesticides. Analysis of residual pesticides in milk, muscle, brain, and fat was performed with a livestock residue test and safety evaluation of the detected pesticide was performed. Efficacy of organic solvent extraction and clean-up of feed was verified, and suitability of the instrument was examined to establish if they are effective, rapid, and safe. This review discussed extensively how pesticide residue tolerance in livestock feed and hazard evaluation may be applied in future studies.