레드 톱 밴트그래스의 성숙종자로부터 최적조직배양조건을 확립하기 위하여 종자로부터 배발생 캘러스 유도 및 식물체 재분화 체계를 확립하였다. 종자로부터 배발생 캘러스 유도시 첨가되는 auxin으로는 2,4-D가 가장 효율적이었으며, 2 mg/L의 2,4-D가 첨가된 MS 배지에서 가장 높은 빈도로 배발생 캘러스가 유도되었다. 배발생 켈러스로부터 식물체 재분화는 0.5mg/L의 2,4-D와 2 mg/L의 BA가 첨가된 N6 배지에서 배양했을 때 64.4%의

Objetive : The cold feeling disease is feeling a cold at normal temperature in specific part of body and it can be felt by oneself and the others. This disease let the person who has the disease take various inconvinience in daily life but they don't take any specific care and actucally there are not efficient care. In this study we took Tae-gye(KI3) which is one of the nine point that circulating energe to whole body(回陽九針穴) and observe the efficiency conpare with non acupoint Methord : We used infra-red thermometer to measure the temperature of hand and arm. Acupuncture is treated using Park-sharm device for double-blinding test. Temperature of PC8 is compared with LU4 for measure the coldness of hand. Results : Taking the temperature of Before-state, average temperature of PC8 is lower then LU4 at 0.3℃ degree. Right about acupuncturing, temperature of PC8 got lower at 0.238℃ degree comparing LU4 in treatment group and , got higher at 0.5℃ degree in control group, but both of them have no significance(p>0.05) During the acupuncturing, temperature of PC8 got higher at 0.388℃ degree then LU4 in treatment group and got higher at 0.975℃ degree in control group, but both of them have no significance(p>0.05). After remove the acupuncture, temperature of PC8 got lower at 0.013℃ degree then LU4 in treatment group and got higher at 0.713℃ degree in control group, but both of them have no significance(p>0.05). In control group, the differences of change are very intense for each grade and they had large standard deviation. In treatment group the differences are decreased gradually and they had small standard deviation(SD=0.424) compare with control group (SD=0.723) but there are no significant difference. Conclusion : The results suggested that KI3 can decrease the difference of temperature between PC8 and LU4, and reduce the coldness of hand. After this, study with more group is needed to verify the possibility.

Objective & Methord : The aim of this study is to examine the effects of indirect moxibustion at the PC6(Naegwan) on normal human beings using power spectrum analysis. Electroencephalogram(EEG) power spectrum exhibits site-specific and state-related differences in various frequency bands. 8 channels EEG study was carried out in 10 subjects(3 females and 2 males). Result : Average value of alpha-wave is decreased significantly in moxa treatment group of real acupoint(PC6) comparing 'before' and 'during' , 'before' and 'after' of the experiment. In non-acupoint(near LU5) group, Average value of alpha-wave is decreased significantly comparing 'during' and 'after'. Alpha value of Both group was decreased according to the degree and the difference is rapid in 'during' - 'after' block. Compare the treat of real acupoint with non-acupoint, there was non significant difference. Conclusion: These results suggested that moxa treatment can revitalize normal human and the influence moxa treatment is more powerful then acupoint-nature. For these reason, usage of substitute moxa for acupuncture is not reasonable.

Trace analysis of Cd and Pb at surface modified thick film graphite electrode with Bi nanopowder has been carried out using square-wave anodic stripping voltammetry (SWASV) technique. Bi nanopowder synthesized by gas condensation (GC) method showed the size of nm with BET surface area, . For a strong adhesion of the Bi nanopowder onto the screen printed carbon paste electrode, nafion solution was added into Bi-containing suspension. From the SWASV, it was found that the Bi nanopowder electrode exhibited a well-defined responses relating to the oxidations of Cd and Pb. The current peak intensity increased with increasing concentration of Cd and Pb. From the linear relationship between Cd/Pb concentrations and peak current, the sensitivity of the Bi nanopowder electrode was quantitatively estimated. The detection limit of the electrode was estimated to be and for Cd and Pb, respectively, on the basis of the signal-to-noise characteristics (S/N=3) of the response for the solution under a 10 min accumulation.

본 시험은 사료용 옥수수 검은줄오갈병 피해 다발지역의 발병실태를 조사하여 종합적인 방제기술 개발에 필요한 기초 자료를 확보하고자, 2006년부터 2007년까지 제주도를 제외한 전국을 대상으로 수행하였다. 검은줄오갈병의 발생은 지역에 따라 차이가 많았으며, 발병이 심한 지역은 경기(이천), 전북(고창, 김제), 전남(영광), 경북(김천, 영주), 충남(천안, 보령)이었다. 옥수수 검은줄오갈병 다발지역에서 파종시기가 4월 26일5월 15일까지 파종이 발병이

The effect of silver ion solution on the growth of Microcystis aeruginosa UTEX 2388 (cyanobacterium) and Chlorella sp. KCTC AG20136 (green alga) was investigated using separated and mixed culture in filtered natural water and BG11 medium. In separated cul

본 시험은 이탈리안 라이그라스 내한 중생 신품종을 육성하기 위하여 1999년부터 2006년까지 축산과학원 조사료자원과에서 수행되었다. 이탈리안 라이그라스 신품종 '코윈마스터'는 2배체 품종으로서 엽의 색은 담녹이며, 월동전 초형은 중간형, 봄의 초형은 반직립형이다. 코윈마스터는 5월 13일경에 출수하는 중생 품종이었다. 특히, 신품종 코윈마스터는 Florida 80 품종과 지엽의 폭은 비슷하나 지엽의 길이가 3 cm 정도 길며, 초장은 89 cm로 Fl

본 연구에서는 버즈풋 트레포일의 Empire 품종을 이용하여 조직 절편체의 종류 및 식물생장조절물질 첨가가 캘러스로부터 식물체 재분화에 미치는 영향을 조사하였다. 각각의 잎과 줄기 절편체를 배지에 치상한 후 배양한 결과, 모든 처리구에서 5-7일 후부터 캘러스 형성이 시작되었다. 캘러스를 유도함에 있어 첨가되는 auxin류로 NAA를 첨가 우수한 결과가 나타났다. 1 mg/L NAA와 1 mg/L BA가 첨가된 CM4 배지에서 잎 절편체 92%, 줄기

티모시의 colt 품종을 이용하여 최적 조직배양조건을 확립하기 위하여 성숙종자를 배양함에 있어 첨가되어지는 식물 생장조절 물질의종류와 농도가 배발생 캘러스 유도 및 식물체재분화에 미치는 영향을 조사하였다. 배발생 캘러스 유도시 첨가되는 2,4-D는 3 mg/L 첨가 구에서 78%로 캘러스 유도율이 가장 높았으며, 3 mg/L 2,4-D와 0.1 mg/L의 BA를 첨가하여 배양했을 때 형성된 캘러스는 조직적으로 치밀하며 유백색의 배발생 캘러스가 가장 많이