본 시험은 국내 TMR 배합소에 대한 실태조사를 통하여 문제점을 파악하고 개선방향을 제시하기 위하여 수행하였다. TMR 배합소 20개소 조사결과 조사 배합소의 65%인 13개소가 사료배합비 작성을 외부에 의뢰하고 있는 것으로 조사되었으며, 배합비 작성 등 컨설턴트 비용으로 월 평균 만원 정도를 지출하고 있어, 컨설팅 비용에 대한 부담으로 경영비를 상승시키는 요인이 되는 것으로 나타났다. 국내 유통 TMR에는 에너지가인 TDN가를 표기하고 있지 않아 농가

본 연구는 시료 및 스펙트럼의 전처리 방법이 근적외선 분광법을 이용한 옥수수 사일리지의 화학적 조성분의 예측능력에 미치는 영향을 평가하기 위해 수행되었다. 시료의 전처리 방법은 건조하여 분쇄하는 방법(Oven Dried Grinding), 액화 질소처리 후 분쇄하는 방법(Liquid Nitrogen Grinding) 그리고 생사일리지(Intact Fresh)처리로 하였으며 4개의 스펙트럼의 수처리(1,4,4, 2,6,4, 2,10,5) 방법을 이용하여

This study was conducted to evaluate roughage to concentrate ratio on the changes of productivity and metabolic profiling in milk. Six lactating Holstein cows were divided into two groups, T1 group was fed low-concentrate diet (Italian ryegrass to concentrate ratio = 8:2) and T2 group was fed high-concentrate diet (Italian ryegrass to concentrate ratio = 2:8). Milk samples were collected and its components and metabolites were analyzed by 1H-NMR (Nuclear magnetic resonance). The result of milk components such as milk fat, milk protein, solids-not-fat, lactose and somatic cell count were not significantly different between two groups. In carbohydrate metabolites, trehalose and xylose were significantly higher (P<0.05) in T1 group, however lactose was not significantly different between two groups. In amino acid metabolites, glycine was the highest concentration however, there was no signifi-cant difference observed between two groups. Urea and methionine were significantly higher (P<0.05) in the T2 group. In lipid metabolites, carnitine, choline and O-acetylcarnitine there were no significant difference observed between the two groups. In benzoic acid metabolites, tartrate was significantly higher (P<0.05) in T2 group. In organic acid metabolites, acetate was significantly higher (P<0.05) in T1 group and fumarate was significantly higher (P<0.05) in T2 group. In the other metabolites, 3-methylxanthine was only significantly higher (P<0.05) in T2 group and riboflavin was only significantly higher (P<0.05) in T1 group. As a result, milk components were not significantly different between two groups. However, metabolites concentration in the milk was significantly different depends on roughage to concentrate ratio.

The present study investigated an ethanol extract (HS0608) of a mixture of three medicinal plants of Curcumalongae radix, Phellinus linteus, and Scutellariae radix for possible neuroprotective effects on neurotoxicity induced by amyloid βprotein (Aβ) (25-35) in cultured rat cortical neurons and antidementia activity in mice. Exposure of cultured cortical neurons to10µM Aβ (25-35) for 36h induced neuronal apoptotic death. At 1-50㎍/㎖, HS0608 inhibited neuronal death, elevation of intra-cellular calcium concentration ([Ca2+]i), and generation of reactive oxygen species (ROS) induced by Aβ (25-35) in primary cul-tures of rat cortical neurons. Memory loss induced by intracerebroventricular injection of ICR mice with 15 nmol Aβ (25-35) wasinhibited by chronic treatment with HS0608 (25, 50 and 100㎎/㎏, p.o. for 7 days) as measured by a passive avoidance test. Fromthese results, we suggest that the antidementia effect of HS0608 is due to its neuroprotective effect against Aβ (25-35)-inducedneurotoxicity and that HS0608 may have a therapeutic role in preventing the progression of Alzheimer’s disease.