Rice is the most important crop as the staple food and two priorities in rice production are high yield and good quality in Korea. Far more improvements in grain quality, especially eating quality are required to meet the demand of consumers in rice producing areas. In the study presented here, a recombinant inbred lines (RILs) population derived from a cross between a temperate japonica and a tropical japonica and its genetic linkage map were employed to locate the QTL locus underlying six parameters of RVA profiles. Out of six RVA profile parameters, two characteristics, breakdown viscosity (BDV) and setback viscosity (SBV) are more correlated to eating quality of cooled rice. A total of four QTLs for two RVA profile parameters were identified. Two QTLs, qBDV-6 and qBDV-9 for BDV were detected on chromosomes 6 and 9. These QTLs increased the BDV by 26.2 and 16.4 from Ilpumbyeo allele, respectively. A QTL, qBDV-6 in the interval RM540-RM587 of the wx gene on chromosome 6 was reacted as a major QTL which could explain 26.2% of the total phenotypic variation. A QTL, qBDV-9 in the interval RM5688-RM444 explained 7% of the total variation as a minor QTL. Two QTLs, qSBV-6 and qSBV-9 for SBV also identified at the same region with QTLs of BDV on chromosomes 6 and 9. A QTL, qSBV-6 in the interval of the wx gene on chromosome 6 could explain 24.4% of the total phenotypic variation. A QTL, qSBV-9 in the interval RM5688-RM444 explained 8.2% of the total variation. These QTL region on chromosomes 6 and 9 would be use as useful marker to select elite lines of good eating quality in early generations in japonica rice breeding.

This experiment was carried out to find suitable sample type for a more accurate prediction and non-destructive way in the application of NIRS technique for estimation amino acid composition of soybean by comparing three different sample types, single seed, whole seeds, and milled seeds’ powder. The coefficient of determination in calibration and 1-VR of cross-validation for 17 amino acids analyzed by NIR using milled powder were highest, followed by single seed, and then whole seeds were the lowest. The R2 coefficients of determination in calibration for single bean perimeters were higher than those of whole beans and showed higher R2 coefficient than 0.8 with the exception of seven amino acids. On the other hand, calibration equation development for only glutamic acid analyzed NIRS data scanned with whole seeds showed higher R2 coefficient than 0.8. Eleven different amino acids, such as aspartic acid, threonine, serine, glutamine, glycine, alanine, valine, isoleucine, leucine, arginine and proline, of soybean seeds in the powder had higher R2 coefficient of determination in calibration than 0.8 and could be estimated for the most accurate prediction. However, judging from the results of single bean samples, in consideration on NIR application for more nondestructive and faster prediction, these amino acids using single bean sample could be estimated without grinding the seeds.

Kjeldahl method used in many materials from various plant parts to determine protein contents, is laborious and time-consuming and utilizes hazardous chemicals. Near-infrared (NIR) reflectance spectroscopy, a rapid and environmentally benign technique, was investigated as a potential method for the prediction of protein content. Near-infrared reflectance spectra(1100-2400 nm) of coarse cereal grains(n=100 for each germplasm) were obtained using a dispersive spectrometer as both of grain itself and flour ground, and total protein contents determined according to Kjeldahl method. Using multivariate analysis, a modified partial least-squares model was developed for prediction of protein contents. The model had a multiple coefficient of determination of 0.99, 0.99, 0.99, 0.96 and 0.99 for foxtail millet, sorghum, millet, adzuki bean and mung bean germplasm, respectively. The model was tested with independent validation samples (n=10 for each germplasm). All samples were predicted with the coefficient of determination of 0.99, 0.99, 0.99, 0.91 and 0.99 for foxtail millet, sorghum, millet, adzuki bean and mung bean germplasm, respectively. The results indicate that NIR reflectance spectroscopy is an accurate and efficient tool for determining protein content of diverse coarse cereal germplasm for nutrition labeling of nutritional value. On the other hands appropriate condition of cereal material to predict protein using NIR was flour condition of grains.

Pearling, an important primary process on food barley utilization, refers to the gradual removal of grain tissues starting from the outer grain tissues/layers, bran, and germ. The removal of barley bran through pearling yields a bright white kernel that is ideal for various food applications. The removed grain layers are usually sold as feed. However, its use for producing various innovative food products such as high-fiber functional pasta. Here we investigated composition of pearled barley. Barley grains from two hull-less varieties, Saechalssal and Hinchalssal, were pearled to various degrees(10-60%). The composition (protein, β-glucan, fat, ash, total phenol and proanthocyanidin) of pearled barley fractions flour(PF) was determined. Protein, fat, ash, total phenol and proanthocyanidin content was decreased in PF according to increasing pearling degree. Hunter L value and whiteness was increased. β-glucan content also was increased from 3.35, 2.73% to 8.9, 9.3% in Sachalssal and Hinchlssal individually. PF of Sachalssal and Hinchalssal showed different content in all components. Protein content was the highest in 90～80% flour fraction. Total phenol, proanthocyanidin and crude fat content was the highest in outer grain layer, bran(100～90% fraction) than other fractions.

We previously reported that reverse transcription-polymerase chain reaction/restriction fragment length polymorphism (RT- PCR/RFLP) was an effective method to identify SMV strains. Using this method a new SMV strain G6H was successfully identified. To introduce resistance locus Rsv4 of V94-5152, we made crosses between two parents, Hwanggumkong and V94-5152, and obtained 6 BC3 F3 progeny lines, which have different size of DNA fragment of Rsv4 locus region. To confirm the virus resistance of progeny lines, artificial inoculation were conducted with 10 SMV strains, G1-G7, G7A, G6H, and G7H. Genomic DNA of tested lines was extracted and used marker genotyping using 9 SSR marker, which covered about 20 cM genetic distance including Rsv4 locus. In the virulence test, only two progeny lines showed resistance to all the SMV strains like a V94-5152. However, the other lines showed necrotic symptoms to G6H strain. It is considered that a minor gene is located near the Rsv4 locus between Satt157 and Sat_254 marker which interacts with G6H. A new strain can be a clue to find a minor gene in the SMV resistance soybean breeding.

Soybean (Glycine max(L.) Merr.) is an important source of high protein and oil. Use of soybean meal bythe food industry is increasing, but severely limiting dietary choices and the quality of life of food-allergic individuals. Gly m Bd 30K (P34) is known as the main seed allergens in soybean-sensitive patients. The objective of this work was to determine the molecular basis of the low mutation of soybean P34 and to design polyclonal antibody for the selection of the causative mutations for wild homozygous, heterozygous and mutant homozygous. Using soybean genome assembly, we knew that soybean P34 genes are existing 2 copies in LG A1 and 1 copy in LG A2 in soybean genome. Actually, we confirmed that 3 copies for P34 gene were existed on soybean genome with Southern blot analysis. Glyma08g12270 of those was expressed at significantly higher level compared with Glyma08g12280 and Glyma05g29130by RT-PCR and western analysis. However this gene was not expressed in the low-P34 germplasm accessions. We develop a co-dominant marker based on the sequence of Glyma08g12270 containing a four-base pair insertion at the P34 start codon. Also, we make a polyclonal antibody for investigation of P34 protein levels. Further study, we will perform the crossing between low P34 accession and elite variety, backcrossing and allergen test using low P34 line.

Soybeans X soybeans mosaic virus (SMV) strains interactions affected plant growth and seed transmission. Strain virulence of SMV depended on host cultivars. Kwangankong and Tawonkong were susceptible to G7H and G5 strains, causing mosaic symptoms. The distribution patterns of two SMV strains in soybean plants inoculated with G7H, G5 and G7H/G5 sets were investigated by RT-PCR/RFLP analysis. In the first treatment, two primary leaves in a single plant were infected with both strains by means of one strain per leaf. The leaves of Kwangankong and Tawonkong at V2, V4 and V6 stage were doubly infected with the two strains and the upper leaves than those had only G7H strain. Secondly, the two soybeans were inoculated with G7H, and 24 h after followed by the other strain inoculation. The leaves of V6 and V8 stages in all infected plants showed mosaic symptoms caused by G7H, and there was no detection of G5 strain. In contrast, the reverse treatment with G5 and G7H induced different results. Pre-inoculated G5 strain detected in every stage besides G7H strain. Host X SMV strain compatibility influenced seed coat mottling, yield, plant height, number of pod per plant. G7H had a seed mottling rate of 98.5% in Kwangankong, while G5 had an incidence of seed mottling of 1.4% in the same cultivar. G5 was more virulent to Kwangankong and had a lower affinity for infecting soybean seed mottling. Additional inoculation of G7H protected soybean yield and growth from G5-inducing loss in Kwangankong.

In April 2009 two wheat cropping fields, Jeonju and Gimje in Jeonbuk Province, showed yellowish leaves and dead tillers in stem bases with sharp lens-shaped darken lesions. The disease incidence in wheat cultivar Jopummil ranged from 2.2 to 43.5%, with a mean incidence of 28.5%. The different incidence was related the seeding date. Earlier seeding (15th October) fields showed severe incidence as 25.5 to 43.5% while late seeding around the end of October weakened incidence in the cultivar. Based on morphology and pathogenic characteristics, the fungus was identified as Rhizoctonia cerealis that causes sharp eyespot in wheat. The disease severely affected growth and yield including culm length, spike length, number of kernel. The average culm length of the infected cv. Jopummil was 66 cm while that of healthy plant was 74 cm. The number of kernel and tiller per unit area was decreased only in the case of more than 13% incidence, but the length of heads was not significantly different compared with that of the healthy plants.

Maize is expected to be planted in June after harvest of winter barley for the double cropping of forage maize-barley. But maize yield tends to be reduced rapidly with late planting after mid-May, so the pre-requisite of maize variety for the double cropping of maize-barley is less reduction of growth and yield at the condition of late planting in June. In order to select domestic forage maize variety adapted to late planting in June after barley harvest, Kwangpyeong-ok, Gangda-ok and Jangda-ok in 2007 and Kwangpyeong-ok, Gangda-ok and Cheongan-ok in 2008 were planted on June 13 and June 21, and plant growths and yields were compared with early planting on April 24 and May 31, respectively. In 2007, Ear number per plant was as high as 0.98 at Kwangpyeong-ok compared to 0.89 and 0.56 of Gangda-ok and Jangda-ok, respectively, at late planting on June 13. TDN and grain yield of Kwangpyeong-ok were the highest among three variety as 1,037 and 710 kg/10a, and yield reduction of Kwangpyeong-ok compared to early planting were 24% and 28 %, which were 7% and 8% lower than Gangda-ok and 22% and 50% lower than Jangda-ok, respectively. In 2008, TDN and grain yield at late planting on June 21 of Kwangpyeong-ok were also the highest among three varieties as 1,157 and 854 kg/10a at late planting on June 24, and yield reduction of Kwangpyeong-ok by late planting were 21% and 19%, which were 10% and 11% lower than Gangda-ok and 22% and 50% lower than Jangda-ok, respectively. It was concluded that proper maize variety adapted to late planting for the double cropping of forage maize-barley was Kwangpyeong-ok because of its higher ear bearing, less reduction of TDN and grain yields at the condition of late planting.