The dynastid beetle Allomyrina dichotoma has been used as a herbal medicine. Recently, we performed de novo RNAsequencing of Allomyrina dichotoma and identified several antimicrobial peptide candidates based on in silico analysis.Among them, cationic antimicrobial peptide, Allomyrinasin, was selected and we assessed the anti-inflammatory activitiesof Allomyrinasin against mouse macrophage Raw264.7 cells. The results showed that Allomyrinasin decreased the nitricoxide production of the lipopolysaccharide-induced Raw264.7 cells. In addition, quantitative RT-PCR, ELISA and Westernblot analysis revealed that Allomyrinasin reduced cytokine expression levels in the Raw264.7 cells. Taken together, thesedata indicated that Allomyrinasin had anti-inflammatory activity in the lipopolysaccharide-induced Raw264.7 cells.

The soybean bug, Riptortus pedestris, is a polyphagous species and an important pest of soybean. We investigated the development periods and survivorship of R. pedestris egg and nymph stage. The study was conducted at eleven constant temperatures of 12, 14, 16, 18, 20, 24, 28, 32, 34, 35, and 36℃, respectively. Egg could not hatch under 14℃. Nymph could not develop to adult at 16℃. The lower developmental threshold (LDT) and thermal constant (K) of R. pedestris nymph stage were estimated by linear regression. Higher temperature threshold (TH) and lower temperature threshold (TL) were calculated by two nonlinear functions (Performance and SSI model). The adult emergence frequency of R. pedestris over full range of constant temperatures was simulated using developmental rate functions and Weibull function.

Riptortus pedestris is a polyphagous species and an important pest of soybean. We investigated the phenology of R. pedestris at 14 locations - Jeonju, Naju, Goheung, Namhae, Milyang, Gunwi, Andong and 7 areas of Jeju. Commercial pheromone monitoring traps were used. The traps were baited with lures containing a 1:5:1 ratio of myristyl isobutyrate, (E)-2-hexenyl (E)-2 hexenoate and (E)-2-hexenyl (Z)-3-hexenoate. There was difference the first adult catch and nymph emergence among locations. To develop spring emergence distribution pattern, we used the trap data from each location. Spring emergence by sampling date was cumulated and analyzed relative to the Julian date using Weibull distribution function.

Programmed cell death (PCD) is decisive in eliminating affected cells in human cancers, whereas there are increasing cases of cancer-related death due to side effects of modern treatment methods. There is an urge for new methods of growth inhibition and elimination of cancer cells with a lower cytotoxicity to normal cells. Irregularity along PCD pathways plays a crucial role in cancer cell carcinogenesis. Apoptosis is a distinct cell death mechanism occurring in multicellular organisms and also called type one programmed cell death. Autophagy and paraptosis are non-apoptotic PCD occurring in multicellular organisms. Natural compounds are the fundament of pharmacological treatments, and flavonoids are natural polyphenolic compounds which are unique due to their diverse chemical structures and various biological active mechanisms like anticancer, anti-inflammatory, antioxidative and much more. This gives an increasing number of studies indicating that some flavonoids from medicinal plants could be promising candidates for new natural anticancer drugs, which attract high interests of academic researchers and advanced users. An understanding of the underlying mechanism of PCD induced by flavonoids in cancer cells is important as it plays a pivotal role in the pathogenesis of many diseases. This systematic review is to report flavonoids and their derivatives as new anticancer candidates to stimulate PCD with a different mechanism based on the pharmacological evidence.

The spring emergence of individual insect would be influenced by surface temperature during winter season. The oriental fruit moth, Grapholita molesta, overwinter as a full-grown larva within spherical and compactly woven cocoons in the clefts of tree bark. It consume new shoots early and fruits later in the growing season. To estimate the spring emergence of G. molesta, we used the lower developmental threshold (8.14°C) and thermal constant (279.3) for overwintering G. molesta. The climate data were obtained from the web site of the Korea Meterological Administration(KMA) from 1975 to 2015 at 61 locations, respectively. The spring emergence of G. molesta was changed depending on ambient temperature of each year.

Climate change can affect major changes to the dynamics of individual insect. The increase of surface temperature would cause multivoltine species to increase the number of generations (i.e. voltinism) per year. Determining the voltinism was based on the climate data and biological parameters. The soybean bug, Riptortus pedestris (Fabricius) (Hemiptera: Alydidae) is one of major pests of soybean and has multi host plants. To estimate the voltinism of R. pedestris, the climate data were obtained from the web site of the Korea Meterological Administration(KMA) from 1966 or 1973 to 2015 at 77 locations, respectively. We used the lower developmental threshold (15.0°C) and thermal constant (312.5DD) for R. pedestris from egg to adult emergence. Degree-days were calculated by using simple cutoff method and cumulating degree-days (CDD) were obtained by summing calculated degree-days. The voltinism of R. pedestris was calculated by dividing CDD per specific period by the thermal constant requirement of R. pedestris development from egg to adult emergence. The number of generations varied depending on the season, with the lowest in February and the highest in August at each location. At least one generation per month would be possible from July to August. The average voltinism from 1966 to 2015 was highest at Seoguipo, and was followed by Daegu, Jejusi, Gwangju and Jeonju. The average voltinism from 1966 to 2015 was lowest at Ulreundo. The voltinism of R. pedestris observed the last 50 years was attributable to human activities at some locations.

The present in vitro study was conducted to examine the effect of buffer solubility of eight protein feeds (coconut meal, distillers grain, sesame meal, perilla meal, soy source cake, rape seed meal, soybean meal and lupine) on the fermentation characteristics, degradability of dry matter (DM) and crude protein (CP), and methane (CH4) production by rumen microbes. Buffer extraction increased pH (P<0.05 ~ p<0.001) of the culture solution but tended to lower ammonia- N concentration for all protein feeds. Total volatile fatty acids(VFAs) and each VFAs concentrations in all incubation was decreased by buffer extraction (P<0.01 ~ P<0.001). Also, molar proportion of acetate in 1h (P<0.001), 3h (P<0.01) and 12h (P<0.05) incubations and molar proportion of propionate in 1h (P<0.001), 3h (P<0.01), 6h (P<0.05) and 12h (P<0.05) were decreased by buffer extraction. But molar proportion of butyrate in 1h (P<0.001), 3h (P<0.01) and 6h (P<0.05) were increased by buffer extraction. The in vitro effective degradability of dry matter (P<0.001) and CP (P<0.001) was decreased by buffer extraction. The methane production (P<0.01~P<0.001) in all incubation was decreased by buffer extraction. The results from in the current study might be useful for diet formulation to improve the feed efficiency of the ruminant animals without massive loss of major nutrients.

The development and fitness of insects depend on the temperature to which they are exposed. The thermal windows are defined as the temperature range between the minimum and maximum rate of development for individual species. The intrinsic optimal temperature for development proposed by Ikemoto is one of important factors that determine the fitness of optimum life history. The temperature requirements for development of 65 species from five orders of insects was obtained from several journals. The minimum and maximum rate of development was estimated using empirical models. The temperature tolerance range of enzyme activation was estimated using Shape-Schoolfield-Ikemoto (SSI) model. The mean and range of intrinsic optimal temperature were 20.89°C and 15.7~27.7°C. The mean intrinsic optimal temperatures of Hemiptera and Endopterygota (Coleoptera, Diptera, Hymenoptera and Lepidoptera) were 20.97°C and 20.71°C. The mean and range of thermal windows were 25.59°C and 16.69~36.13°C. The mean thermal windows of Hemiptera and Endopterygota were 25.53°C and 25.62°C. also not much different. Each species of insects had a limited temperature range for development. It is needed further studies for understanding the ecological, physiological and evolutionary response of insects to their thermal environments.

The growth, differentiation, development and fecundity of insects are influenced by temperature. The relationship between the development rate of insect and temperature has been studied since 1978 in Korea. The relationship between temperature and insect development was published in the Korean Journal of Applied Entomology (71 papers). The contents about Hemiptera, Lepidoptera, Coleoptera, Diptera, Hymenoptera, Acarina and Neuroptera were published in 27, 16, 7, 6, 4, 5, and 2 papers of the journal. Approximately 33 functions from many international journals were published to figure out the relationship between temperature and development rate of insects. The functions have been developed based on two principal ways – simplified analytic method and biophysiological approach. The empirical models are based on the law of total effective temperature and heat summation. The biophysiological models are based on the equations of Arrhenius and Eying. The thermal constant and lower temperature threshold are estimated using linear functions. The minimal and maximal development rate are presented by nonlinear equations. The Sharpe-Schoolfield-Ikemoto (SSI) model showed the intrinsic optimal temperature. Cumulative proportion of development completion for each life stage of insects was analyzed using Weibull and sigmoid functions. We discussed the application and implication of linear and non-linear temperature dependent development models.

본 연구는 섬유소분해 효소제를 공급수준에 따라 비육전기 및 비육후기 한우 거세우에 적용하였을 때 성장 및 도체성적에 미치는 영향을 조사하기 위하여 수행하였다. 시험동물은 비육전기 및 비육후기 거세우로 각각 54두씩 총 108두를 대상으로 하였으며, 시험 개시 시 공시된 비육전기 및 후기 거세우의 평균체중은 각각 397.4±27.2(평균 17개월령), 525.2±47.4kg(23개월령) 이었다. 시험사료는 비육전기사료와 비육후기 사료를 구분하여 대조구 사료에 섬유소분해 효소제 0.05(E0.05, L0.05), 0.15% (E0.05, L0.15)를 첨가한 비육전기 및 비육후기 각각 3처리구에 각 처리당 6마리씩 3반복으로 완전임의배치하여 실시하였다. 비육후기 한우 거세우에서 농후사료 섭취량은 대조구와 L0.15구에서 L0.05와비교하여 증가하였으며, 총 건물섭취량 역시 동일한 결과를 나타냈다(p<0.001). 반면, 비육전기 및 후기 한우 거세우 모두 증체량 및 사료효율은 유의한 차이를 나타내지 않았다. 한편 비육후기 거세우의 도체특성은 L0.05와 L0.15구에서 대조구와 비교하여 지방색이 유의하게 높은 것으로 조사되었다 (p<0.01). 등심조직의 지방산 분석은 대조구와 처리구는 유의한 차이를 나타내지 않았다. 결과적으로 본 시험에 사용된 섬유소 분해효소의 비육기 한우 거세우에 대한 첨가효과는 등심의 지방색이 짙어진 것을 제외하고 없는 것으로 나타났으며, 추후 볏짚 위주의 조사료 급여체계에서 효소제 첨가수준 및 효소역가에 대한 추가적인 연구가 필요할 것으로 판단된다.

The purpose of this study was to determine the effects of swiss ball program training on lower extremity function of old elderly with mild cognitive impairment. The subjects were 30 mild cognitive impairment eldrly people aged between 65 and 80, who were divided into the swiss ball program training group(n=15) and the control group(n=15). The swiss ball program training group engaged in a 50-minute exercise session using. Lower extremity function scale of the two groups were measured before and after the intervention. The results are as follows. According to the swiss ball program training conducted to examine the effects of the training on lower extremity function, Moreover, the two groups showed significant differences in lower extremity function. These results indicate that virtual reality training is effective in improving lower extremity function. To conclusion, swiss ball program was found to have a positive effect on elderly lower extremity function. Swiss ball program training can be proposed as a form of fall prevention exercise for the mild cognitive impairment. Swiss ball program may be helpful to reduce the incidence of dementia and behavioral complications.

The small brown planthopper, Laodelphax striatellus, and the white backed planthopper, Sogatella furcifera, are important agricultural pests. L. striatellus could overwinter in Korean peninsula as nymph stage but S. furcifera could not. We investigated the survivorship and fecundity of two species for comparing population growth. The longevity and fecundity of two species were investigated at temperatures of 12.5, 15.0, 17.5, 20.0, 22.5, 25.0, 27.5, 30.0, 32.5, and 35.0℃. Adult longevity and fecundity of two species were significantly influenced by temperature. Longevities of L. striatellus were longer than those of S. furcifera at different temperatures. Specially, oviposition period of L. striatellus was twice as long as that of S. furcifera. Total fecundity per female of L. striatellus were also more than those of S. furcifera. The oviposition model of each species was consisted of three components - age specific survival rate, age specific oviposition rate and total fecundity per female. The relationship between adult developmental rate and temperatures was described by exponential function. The age specific survival rate and the age specific cumulative oviposition rate were explained by two parameter inversed-Weibull function and Weibull function. Temperature dependent fecundity was estimated using three parameter Gaussian function. Daily egg production of two species in relation to adult age and temperature was estimated.

Coevolution is a large part of evolution and the patterns of adaptation and speciation may be modified by coevolution processes. The arms races between insects and plants in natural system are one of coevolutionary processes and the mechanistic and predictive processes of the system may be helpful to understand the interaction between host plants and herbivores in agricultural systems. Furthermore, human activities may give direct and/or indirect effects on ecological and evolutionary processes in natural and agricultural ecosystems and have selection power as well as natural selection. Recent studies showed that the fundamental principles of evolution – variation (genetic difference, phenotypic plasticity, epigenetic change, and nongenetic inheritance), selection (directional or fluctuating), connectivity and eco-evolutionary dynamics – are important to understand the interplay between insects and plants. The coevolutionary processes between plants and herbivores were also interpreted by the geographic mosaic theory. The evolutionary effects on ecological dynamics in natural ecosystems were classified four categories: trophic specialization, evolution of defense, interaction outcome and loss of traits in absent of interaction. It was reviewed that it is necessary to consider the ecological response in evolutionary studies as well as evolutionary variation in ecological researches.

Papilio macilentus is the longest tail butterfly of Papilionidae found in Japan, China and Korea and has distinctive morphological differences between male and female. P .macilentus feeds on Zanthoxylum schinifolium and has three generations per year. The lower developmental threshold and thermal constant for adult emergence of P. macilentus overwintering pupae were estimated at four different constant temperatures (15, 20, 25 and 30°C) with photoperiod 10:14 (L:D). The lower developmental threshold of both sexes combined was 7.95°C. The development of overwintering pupae decreased with increasing temperature between 15 and 30°C. Thermal constant of both sexes combined was 251.26DD. The relationship between the developmental rate and temperature was fitted by linear and five nonlinear developmental rate models (Logan 6, Lactin 1, 2 and Briere 1, 2). The development of overwintering P. macilentus pupae was described by the two-parameter Weibull distribution model. The temperature-dependent adult emergence model of P. macilentus developed in this study could be used to predict the full-cycle phenology model of P. macilentus and applied to understand the effects of climate change on the emergence distribution of overwintering pupae.

Grapholita dimorpha is a new pest of apple, pear and plum in Japan and Korea as well. Although G. dimorpha was reported as a multi-voltine insect with four generations per year, the phenology is not fully known in Korea. We present a model to predict the spring occurrence of G. dimorpha adults based on the relationship between the cumulative proportion of G. dimorpha adult catches and accumulated degree-days. Five different distribution models were applied and the selection was made based on the statistical information criteria (AICC and BIC). Model validation was performed with the field data of the male moth catches in sex pheromone-baited traps of two apple production regions (Chungju and Geochang) of Korea in 2011. Model predictions of the dates for the cumulative 50% male moth catches were within five days variation. Because G. dimorpha has a feeding habit of boring inside the fruit the management practice of G. dimorpha should apply on egg and neonate larval stage. The management strategy of G. dimorpha in spring might be improved by the model.

The study was conducted to develop the full seasonal phenology model of G. molesta in pear orchard. G. molesta is a multi-voltine insect with four to five generations per year depending on temperature, food resources and geographic location. For precise model construction, information on temperature-dependent development and the distribution of developmental completion of overwinteirng and summer G. molesta population was used. The performance of model was based on single cohort population of G. molesta. The validation of model system was performed with the male moth catches in sex pheromone-baited traps of pear orchards in four pear production regions (Anseong, Icheon, Naju and Ulju), three apple production regions (Andong, Chungju and Geochang), and four plum orchards (Uiseong) of Korea in 2010 and 2011, respectively. The observed phenology of seasonal population of G. molesta was explained by our model system. The predicted dates for the cumulative 50% male moth catches per each generation were within seven days variation per each generation. The precise predictive model of G. molesta adult occurrence could help decision making and enhancing control efficacy.

Pollinating insects function significantly in structuring ecosystem as well as agricultural production. Among the variety of pollinators, Osmia cornifrons is one of the important pollinators in apple orchards. Unlike honey bees, O. cornifrons is able to pollinate at low temperature that facilitates its wide commercial use. The female O. cornifrons in nature lay their eggs to the interior of the bamboo reeds where they spend their larval and pupal stage throughout the summer and during fall they overwinter as adult. The adult bee emerges from the overwintering cocoon in the spring seasons. Since the complete synchrony with flowering period is critical, farmers collect and store the dormant state of O. cornifors in cold storage and expose them in due time to outdoor temperatures for their emergence, pollination activity can be performed. However, information on biological and ecological requirements for the emergence of overwintering adults are rarely reported. We carried out the experiments on temperature dependent adult emergence and constructed the emergence model based on developmental rate and distribution. Parameters were estimated from linear model and weibu

This study was conducted to examine the ability of the predacious mite G. occidentalis to limit population density of T. urticae infesting roses in the greenhouse, Washington state university, Pullman, U.S.A. Population fluctuations of T. urticae, G. occidentalis, and Macrosiphum euphorbiae were assessed for over two years on greenhouse roses through time series data. During the first half of experiment a stratified random sampling was performed (from May 2000 to May 2001) and the presence/absence and randomized sampling method was utilized during second half (from June 2001 to September 2002). Populations of T. urticae and G. occidentalis were estimated by R-functions or discrete prey-predator equations. During 121 weeks there were six population fluctuations of T. urticae, G. occidentalis and M. euphorbiae. The variation of G .occidentalis was closely associated with that of T. urticae but G. occidentalis did not overexploit T.urticae. The predicted populations of both mites were explained by above equations. M. euphorbiae and T. urticae might be compete for limited resource in the greenhouse rose and the movement of G. occidentalis was inhibited by the honeydew of M. euphorbiae. Therefore, M. euphorbiae showed negative relationship with T. urticae and G. occidentalis from the results of non-linear regression analysis. Although the relationship between T. urticae and G. occidentalis was negative it was unlikely to present complete biological control of T. urticae on greenhouse roses. It might be necessary an integrated management strategy to control T. urticae in bent-cane greenhouse roses.