전기영동법과 발색반응법 등의 장, 단점 분석을 통해 가장 저렴한 비용으로 신속, 정확하게 lipoxygenase isozymes를 검정하는 분석체계를 확립하여 무비린내 나물콩 품종육성에 활용 가능할 것으로 보인다. 1. SDS-PAGE 전기영동법은 Lx-0, L-1, L-2, 및 L-123의 검정에는 효과적이었으나, L-3, L-13, 및 L-23의 검정에서는 분리능에 있어서 정확도가 떨어져 검정효율은 92.2%를 나타내었다. 2. 발색반응법을 이용한 lipoxygenase isozymes의 검정방법은 간편하고 신속하였으며 검정효율은 95.7%였다. 3. L-1과 L-2가 존재하는 종실의 기질용액에서는 1분 이내에 methylene blue의 청색 반응용액을 탈색시켰으며 그 결과가 오랫동안 지속되었다. 4. L-3가 존재하는 종실의 용액에서는 20초 이내에β-carotene의 황색 반응용액을 탈색시켰으나 그 결과가 오래 지속되지 않아 신속한 결과분석이 요구되었다. 5. L-1, L-2 및 L-3을 동시에 검정하기 위한 전체 시료량은 콩 종자 1알의 약 10 mg 정도이면 충분하였으며, 검정하지 않은 나머지 부분은 파종에 이용될 수 있으므로 F2 종자에서 lipoxygenase isozymes 결핍계통을 조기에 선발 가능하였다. 6. 발색반응법과 SDS-PAGE 전기영동법을 적절히 겸용하여 수행하게 되면 시료 분석에 소요되는 시간은 1인 100점에 6.5시간, 재료비는 약 54,000원이 소요되어 이 시스템이 가장 효율적인 lipoxygenase 검정방법으로 판명되었다.

A new sprout-soybean cultivar, "Pungwon" was developed at the Honam Agricultural Research Institute (HARI), and National Institute of Crop Science (NICS) in 2006. "Pungwon" was selected from a cross between S193001 and Suwon 164. The preliminary, advanced, and regional yield trials for evaluation and selection of this line (Iksan 52) were carried out from 2002 to 2006. This cultivar has a determinate growth habit, white flower, grayish brown pubescence, yellow seed coat, yellow hilum, lanceolate leaflet shape and small seed size (10.9 grams per 100 seeds). The maturity date of "Pungwon" is seven days earlier than that of the check variety, "Pungsan". It has a good seed quality for soybean-sprout. The soybean-sprouts grown from "Pungwon" have high isoflavone (5,935 μg/g) and amino acid (160 mg/g) contents. This cultivar has resistance to soybean mosaic virus (SMV), necrotic symptom (SMV-N), and other most troublesome soybean diseases which are bacterial pustule and black root rot. Especially, "Pungwon" has useful characteristics, such as lodging and shattering resistance. The average yield of "Pungwon" was 3.04 ton per hectare, about 7 % higher than the check variety, "Pungsan" at the regional yield trials (RYT) for double cropping.

A new sprout-soybean cultivar, "Jonam" was developed at the Honam Agricultural Research Institute (HARI) in 2006. Jonam was selected from a cross between Eunhakong and Jeonju-I1. The preliminary, advanced, and regional yield trials for evaluation and selection of this line (Iksan 48) were carried out from 2002 to 2006. This cultivar has a determinate growth habit with purple flower, grayish brown pubescence, grayish brown hilum, lanceolate leaflet shape and small seed size (9.6 grams per 100 seeds). The maturity date of "Jonam" is 18 days earlier than that of the check variety, "Pungsan". It has good seed quality for soybean-sprout and resistance to lodging. This cultivar has resistance to soybean mosaic virus (SMV) and necrotic symptom(SMV-N). The average yield of "Jonam" was 2.57 ton per hectare in the regional yield trials for double cropping carried out for three years from 2004 to 2006.

“Shinmichal1”, a waxy red winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross “Alchan”/“Chal2”during 1997. “Alchan” is a soft red winter wheat with high yield and good noodle quality. “Chal2” is a waxy wheat line crossed by “Kanto107” (Wx-A1b and Wx-B1b) and “BaiHuo” (Wx-D1b). “Shinmichal1” was evaluated as “Suwon306” in Advanced Yield Trial Test in 2002. It was tested in the regional yield trial test between 2003 and 2006. “Shinmichal1” is an awned, semi dwarf and soft red winter wheat, similar to “Uri” (check cultivar). The Heading and maturing date of “Shinmichal1” were similar to “Uri”. Culm and spike length of “Shinmichal1“ were 79 cm and 7.8 cm. “Shinmichal1“ had greater test weight (802 g) and lower 1,000-grain weight (31.8 g) than “Uri” (782 g and 35.2 g, respectively). It has resistance to winter hardiness, wet-soil tolerance, lodging tolerance and moderate post-harvest sprouting damage. “Shinmichal1” had higher flour yield (66.2%), ash (0.73%), protein (10.9%) and SDS sedimentation volume (40.7 ml) than “Uri” (64.7%, 0.53%, 9.3%, and 32.5 ml, respectively) and similar flour color to “Uri”. “Shinmichal1” had higher dough extensibility and lower dough strength than “Uri”. It is 5.2% of amylose content and had typical waxy starch pasting properties. Average yield of “Shnmichal1” in the regional adaptation yield trial was 5.24 MT ha-1 in upland and 5.58 MT ha-1 in paddy field, which was 3% and 8% lower than those of “Uri” (5.39 MT ha-1 and 6.09 MT ha-1, respectively). “Shinmichal1” would be suitable for the area above the daily minimum temperature of -10℃ in January in Korean peninsula.

“Hwanggeumchal”, a new covered waxy cultivar derived from the cross between “Chalssalbori” and “Milyang 65” with waxy endosperm and early maturing cultivars, respectively was developed at Honam Agricultural Research Institute (HARI), NICS, RDA, 2006. The origin of “Hwanggeumchal” is “Suwon 403” (SB942090-B-B-B-42-1). The initial cross was made in 1994 and the selected line showed a high yield and good quality characteristics under yield trial test in 2003. “Suwon 403” consistently performed well for three years (2004-2006) from the four locations of regional yield trial (RYT) in Korea and released as “Hwanggeumchal”. The characteristics of “Hwanggeumchal” were the following: rate I growth habit, green leaf and stem, compact spike and with long rough awns. The heading date was April 30 in upland and April 28 in paddy field, which was 1 and 4 days later than that with check cultivar, “Seodunchalbori”. The culm length was 81 cm which was 3 cm shorter than those with check cultivar. It showed spike length of 4.5 cm and 633 spikes per m2, 64 grains per spike, 27.6 g of 1,000-grain weight and 632 g of test weight. It showed stronger winter hardiness and higher resistance to barley yellow mosaic virus (BaYMV) than those with check cultivar. It had yellow aleurone and showed higher water absorption, expansion rate and total phenol content than those of check cultivar, but lower protein, β-glucan content and pearling rate. The average yield of the pearled grain in the RYT was 4.04 ton ha-1 in upland and 4.12 ton ha-1 in paddy field, which was 10% higher and 4% lower than that of the check cultivar, respectively. This cultivar would be suitable for the area above the daily minimum mean temperature of -8℃ in January in Korean peninsula.

“Hyemi”, a new covered cultivar derived from the crosses between “Suwon 298” and “Milyang 34/Albori//Dongbori” developed at the Honam Agricultural Research Institute (HARI), NICS, RDA in 2006. The origin of “Hyemi” is “Suwon 400” (SB951032-B-B-B-28). The initial cross was made in 1995 and the selected line showed a high yield and good quality characteristics under yield trial test in 2003. “Suwon 400” consistently performed well for three years (2004-2006) from the four locations of regional yield trial (RYT) in Korea and released as “Hyemi”. The characteristics of “Hyemi” were the following: rate Ⅳ growth habit, green leaf and stem, compact spike and with long rough awns. The heading date was April 29 in upland and April 23 in paddy field, which was similar and 2 days earlier than that with check cultivar, “Olbori”. The culm length was 81 cm which was 4 cm shorter than those with check cultivar. It showed spike length of 4.1 cm and 646 spikes per m2, 52 grains per spike, 33.4 g of 1,000-grain weight and 672 g of test weight. It showed similar winter hardiness and higher resistance to barley yellow mosaic virus (BaYMV) than those with check cultivar. It showed higher protein content, whiteness and total phenol content than those of the check cultivar, but lower β-glucan content and pearling rate. The average yield of the pearled grain in the RYT was 3.95 ton ha-1 in upland and 4.38 ton ha-1 in paddy field, which was 13% and 16% higher than that of the check cultivar, respectively. This cultivar would be suitable for the area above the daily minimum mean temperature of -8℃ in January in Korean peninsula.