Due to rapid industrialization and population growth uncontrolled release of heavy metals are entered into the waters. Among these heavy metals Pb(II) is one of the major toxic metal and in recent years the production and consumption of lead is increasing worldwide. Pb(II) can be entered to aqueous streams from several industries and can enter into the humans food chain through drinking water and crop irrigation. Lead can causes severe damage to the kidney, nervous system, reproductive system, liver and brain. The permissible level for lead in drinking water is 0.05 mg/l. Thus in recent years a number of methods and materials were developed to removal Pb(II) from aqueous solutions. Among these material bio-chars obtained from plant materials have gained special attention due to their low-cost and abundant nature. In present investigation we have developed magnetic bio-char composite from pine bark. Pine trees are wide spread throughout the South Korea and the bark from pine tree has no commercial use and is available as waste. Thus we have utilized this waste inexpensive material from preparing bio-char composite. The pin bark obtained was initially made into fine powder and washed several times with water and was filtered. To this powder an appropriate amounts of nitrate salts of cobalt and iron dissolved in ethanol solution was added and stirred for 15 minutes. This solution was oven dried at 70℃ and this was further calcined at 900℃ in nitrogen atmosphere. As obtained material was washed several times with water and dried in oven over night. This was used as adsorbent for treating lead contaminated aqueous solutions. As obtained bio-char composite was used to remove Pb(II) from aqueous solutions. Various parameters influencing Pb(II) removal like initial pH, contact time and initial concentration were studied. Effect of pH on Pb(II) removal was studied in the pH range from 2-8 at Pb(II) concentration 10 mg/L using an adsorbent dose of 300 mg. At below pH 3 a lower percent removal was observed whereas above pH 4>90% removal was observed. Further effect of contact time on Pb(II) removal was studied from time range between 10-180 min. Two kinetic models pseudo-first, pseudo-second-order models were used to evaluated the kinetic data and found that the data was better fitted to the pseudo-second-order model. From the overall results it was found that as prepared magnetic bio-char composite prepared from pin bark waste was effective and economic for treating Pb(II) contaminated aqueous solutions.

A field study was conducted to understand nitrogen use efficiency of high yielding Japonica rice varieties under three levels of nitrogen fertilizer (90, 150 and 210 kg N ha -1 ) in Iksan, Korea. Two high yielding rice varieties, Boramchan and Deuraechan, and an control variety, Dongjin2, were grown in fine silty paddy. Nitrogen use efficiencies (NUE) were 83.3, 56.3, and 41.2 in 90, 150, and 210 kg N ha -1 fertilizer level, respectively. Total nitrogen uptake varied significantly among nitrogen levels and varieties. Variety Dongjin2 showed the highest nitrogen uptake efficiency (NUpE), while Boramchan and Deuraechan showed higher nitrogen utilization efficiency (NUtE). However, Nitrogen harvest index (NHI) was higher in Boramchan (0.58) than Deuraechan (0.57) and Dongjin2 (0.53). Rough rice yield showed linear relationship with total nitrogen uptake (R 2 =0.72) within the range of nitrogen treatments. Boramchan produced significantly higher rough rice yield (8546 kg ha -1 ) which mainly due to higher number of panicles per m 2 compared to Deuraechan (7714 kg ha -1 ). Deuraechan showed higher number of spikelets per panicle, but showed lower yield due to lower number of panicle per m 2 . Rice varieties showed different nitrogen uptake ability and NUE at different nitrogen level. Plant breeders and agronomist should take advantage of the significant variations and relationships among grain yield, NUpE, and NUE.

Drought and salinity are the major abiotic stresses which are being continued to hamper the ecosystem and agriculture of the affected region. Plant species have adaptations to enhance their ability to tolerate stresses through physiological adjustment. Therefore, substantial amount of research are ongoing to provide insights about those mechanisms which enlighten the stress tolerance in plant. In this study, several rice cultivars were collected from the different parts of the world and ion accumulation experiments were conducted to select the best stress tolerant cultivar in drought and salt stress environment. For stress treatment, five rice cultivars were subjected to salt (200 mM NaCl) and drought (200 mM Mannitol) for 72h. Later Na+, Ca++, K+ concentrations in shoot and root samples were examined at different time interval. In both drought and salt stress, rice cultivar C201 (collected for uzbekistan) showed the lowest levels of Na+ ion and Na+: K+ ratio compared to other cultivars. It was significant parallel observation with pokkali (known salt tolerant cultivar). In this preliminary study, it was observed the C201 had more stress tolerant in terms of ion accumulation; however detail physiological studies are required to strengthen the idea regarding the best stress tolerant physiotype.

Korean ginseng has been used for thousands of years as an important medicinal plant. Lime-Bordeaux mixture (LBM) was made with copper sulfate and quicklime, which was sprayed instead of pesticides in ginseng field. Net photosynthesis (PN) was compared between Treatment and Non-treatment of LBM in 3 Year Old Ginseng. PN in control plot recorded 2.94μmol (CO2) m-2s-1 at the first day of experiment, which was similar until the last day of experiment. However, The PN in LBM recorded 2.23μmol (CO2) m-2s-1, which was lower than that in control plot. As time goes by, The PN in LBM was gradually increased up to 3.21μmol (CO2) m-2s-1 and finally, it was similar with that in control plot at 7th day as a 3.20μmol (CO2) m-2s-1).

Peritonitis is a common, major complication in continuous ambulatory peritoneal dialysis (CAPD) patients and tuberculous (TB) peritonitis has been reported to constitute 1-2% of all peritonitis cases. In CAPD patients, TB peritonitis is often diagnosed late and has a high mortality rate. In a recent recommendation made regarding the treatment of TB peritonitis, primary removal of a peritoneal dialysis catheter has been advised in addition to anti-TB drugs. Here, we describe two cases of TB peritonitis in CAPD patients successfully treated without peritoneal dialysis catheter removal.

Green roof policies has helped to advance urban greening by creating green spaces on previously unused rooftops. However, there are no requirements covering matters such as greening methods, operation and maintenance obligations and many problems for sustainability and functioning of green roof and green space have been pointed out. This research is intended to clarify the contents and criteria of green roof policies currently implemented in Korea, Japan and other countries, compare them with the evaluation criteria for sustainable and public benefit rating systems and propose comprehensive indices for evaluating the multidimensional functions of green roof from the perspective of urban open space. As a result, it is found that obligation, grant, reimbursement, density bonus and tax credit are frequently used as green roof policies and 41 criteria including percentage of greening area, percentage of trees or shrubs, reduction of heat island effect, open space, landscape, and promotion of biodiversity are identified as criteria applicable to evaluating the multidimensional functions of green roof from the perspective of urban open space, respectively.

In this paper I argue that the so-called gapless relative clause (GRC) in Korean actually has a syntactic gap, and thus, it is a variant of the externally headed regular gappy RC. I also argue that the surface structure of GRC is derived from the underlying structure where the cause-effect relation required in GRC constructions is fully realized via pragmatically conditioned ellipsis. Thus I suggest that the verbal effect part can be ellipted to the extent that this part is pragmatically recoverable in the presence of the head noun that denotes the same effect. The categorial status of the GRC is further claimed to be CP, which then hosts operator movement in its Spec in a usual way. So there is little anomaly in the RCs known as gapless RCs.

Sea Buckthorn (Hippophae rhamnoides L.) has been used in traditional medicine for the treatment of cough, indigestion, circulatory problems and pain. The associated anti-inflammatory effect of this agent is achieved via the inhibition of Nf-kB signaling, a property that has been demonstrated to effectively control the symptoms of various skin disorders, including atopic dermatitis. Accordingly, the purpose of this study was to assess the efficacy of Sea Buckthorn in reducing the production of lipopolysaccharide (LPS) activated nitric oxide (NO) by inhibiting the Nf-kB pathway, as measured by the symptoms of atopic dermatitis (AD) occurring secondarily to inflammation and immune dysregulation. Our data demonstrate that Sea Buckthorn significantly decreased the LPS-induced production of NO (p〈0.001). Atopic dermatitis was induced by repeated application of 2,4-dinitrochlorobenzene to the dorsal skin of mice. Topical application of 5% Sea Buckthorn extract improved the symptoms of AD, specifically reducing disease severity scores, scratching behaviors and epidermal thickness. When compared to the control group, animals treated with Sea Buckthorn exhibited increased serum IL-12 levels and decreased serum TNF-α, IL-4 and IL-5 levels. Such a modulation of biphasic T-helper (Th)1/Th2 cytokines may result in a reduction in serum IgE levels. Our findings suggest that mechanism of action of Sea Buckthorn in the treatment of AD is associated with a marked anti-inflammatory effect as well as an inhibition of Th2-mediated IgE overproduction via the modulation of biphasic Th1/Th2 cytokines. Such results suggest that topical Sea Buckthorn extract may prove to be a novel therapy for AD symptoms with few side effects.

This study attempts to propose the possibility of the sex reversal in Tegillarca granosa and Ruditapes philippinarum by confirming the changes in the sex ratio with the shell length (SL) in the same population level. For analysis of sex ratio with SL, 1500 individuals of T. granosa (SL 10.1-45.0 mm) and 712 individuals of R. philippinarum (SL 15.1-70.0 mm) were used. Sex was analyzed histologically. The average sex ratios (F:M) of T. granosa and R. philippinarum were 1:1.22 and 1:0.96, respectively. However, sex ratio was found to differ when the clams were divided into groups according to SL in 5.0 mm intervals. Both species displayed the tendency of increase in the proportion of female with increase in SL. In this study, changes in the sex ratio in accordance with the growth of T. granosa and R. philippinarum are determined to be indirect evidence that signifies their sex reversal.

Recent release of whole genome draft sequences in legume species have led comparative genome studies among legume plants including Glycine max, G. soja, Cajanus cajan and Medicago truncatula. The majority of comparative genomic researches have been conducted based on synteny of coding sequences and coding sequence variations may be one of major forces for speciation and evolution. However, non-coding sequences have been also reported to be important phenotypic regulators. Especially, since short sequence motifs in the promoter regions are highly conserved, they are suggested to be another resources of interests in comparative studies. In this study, we predicted the conserved short sequence motifs by BLASTN algorithm using dicot promoter database from Softberry (http://www.softberry.com). A total of 37,396 conserved short sequence motifs were identified onto 2 kb upstreams of 46,367 high confident gene model of G. max (cv. Williams 82). Meanwhile, whole genome of 7 soybean landraces (G. max) and 7 wild soybean genotypes (G. soja) were sequenced at low depth of less than ten using Illumina Hiseq 2000. Among these genotypes, nucleotide variations were identified in predicted conserved regulatory motifs by mapping of short reads to the reference genome sequence using the Samtools program (http://samtools.sourceforge.net/). Fifteen and two genes, which have SNPs in regulatory motifs and no SNP in coding sequence, were identified by comparisons of inter-species and intra-species, respectively. qRT-PCR experiments are in progress for investigating differences of these 17 genes expressions at transcriptional level.

Mutagenesis approach in combination with whole genome sequencing has become an import role in genetic and molecular biological study and breeding of crop plants. In this study, we screened the fast neutron M4 10,000 soybean mutant plants based on morphological phenotypes of agronomically important traits and characterized the mutant of interest using resequencing. Fast neutron radiation has been known to be a very effective mutagen to cause large deletion in genome. The screened mutant showed abnormal phenotypes in plant heights, seed sizes, color of leaves, number of leaves, maturity and number of branches etc. Among them, the mutant displaying short plant height and bush type of growth habit was selected for identification of the altered genomic regions. Analysis of deletion sites of genome in interesting soybean mutant was performed using next generation sequencer Illumina Hi-seq. Mutant sequence reads generated by paired-end shotgun library were mapped on a draft soybean reference soybean (G. max cv. Williams 82). The paired-end DNA sequences of 21.6 Gb produced by Illumina Hi-seq produced 21 fold sequence depth. Among the predicted deletion sites, total 3 deletion regions confirmed by PCR. Glyma03g02390 gene and Glyma03g03560 gene were involved in the deletion regions. Glyma03g02390 gene was related to AMP binding, catalytic activity, cofactor binding and metabolic process of cell growth and Glyma03g03560 gene was concerned to oxygen binding, defense response to bacterium, and especially process of indole acetic acid (IAA) biosynthesis. These genes detected in this mutant will be studied about their molecular function in stunted phenotype.

A new peanut variety “Sangan”(Arachis hypogaea ssp. fastigiata L.) showed the highest yielding of Korean varieties was developed at the Department of Functional Crop, NICS, in Milyang in 2011. This was developed from the crossing line between cultivar “Palkwang” with high quality and “local collection” with short stem. “Sangan” which is Shinpung plant type has 44cm of main stem length and 10 branch number per plant. Each pod has two grains with long ellipse shape of brown testa and yield components is composed of 40 mature pods of per plant, 96g of 100-seed weight, 79% of pod shelling ratio in the regional yield trials(RYT). Seed quality showed 48.5% of crude oil and 29.1% of protein content. This variety also showed more resistant to early leaf spot and web blotch, compared with check variety, Daekwang. In the regional yield trials “Sangan” outyielded check variety by 15% with 4.87 MT/ha for kernel.

Breeding the whole crop siliage rice cultivar basically requires priority in high biomass and grain yield and tolerance biotic and abiotic stresses. In this context, AA genome wild rices are potential resources to broaden genetic variability for whole crop silage cultivar. We have developed several introgression populations from crosses with three AA genome wild rices, O.glaberrima, O.rufipogon, and O.longistamina. Among these populations, the three BC4F8 near isogenic lines (NILs) derived from a cross of Milyang 23 and O.glaberrima(acc. 101154) could produce the high-yielding lines surpassing Milyang 23 by 20% in grain yield. Introgression lines from crosses with 4 accessions of O.rufipogon with japonica rices, Hwaseongbyeo and Ilpumbyeo had huge agronomic variability and very tall and vigorous lines possessing culm length of 125 ∼ 187cm were selected as promising potential parents to improve biomass of existing cultivars. These selections with high yield potential and biomass will be crossed to create another gene pool to combine high yield and biomass and anther culture breeding could be useful to develop genetically fixed F2 lines with both traits.

A new vegetable peanut variety “Jaseon”(Arachis hypogaea ssp. fastigiata.) was developed at the Department of Functional Crop, NICS, in Milyang in 2011. It was developed from the cross between the Large grain cultivar “YG60” and the short stem cultivar “YG55”. “Jaseon” which is Shipung plant type has 43cm main stem length and 11 branch number per plant. Each pod has two grains with brown testa with long-ellipse shaped large kernel and dried 100-seed weight was 105g in the regional yield trials(RYT). The Sucrose and tannin content of fresh peanut are 25.0mg/g and 4.4mg/g, respectively. This variety also showed more resistant to late leaf spot, stem rot and lodging, compared with check variety, Palkwang. In the regional yield trials “Jaseon” was outyielded than check variety by 15% with 10.21MT/ha for fresh pod and by 11% with 4.37MT/ha for dried kernel.

Scientific studies have shown that essential fatty acidintake can have a dramatic impact on human health. Soybean [Glycine max(L.) Merr.] oil from current commercial cultivars typically containsaround 8%linolenic acid (18:3) known as omega-3 fatty acid. Omega-3 fatty acid plays an important role to prevent cardiovascular disease and cancer. Relatively high 18:3 content in seed oil is a trait of the wild soybean (Glycine soja Sieb. and Zucc.) ancestor of modern soybean cultivars. Wild soybean is native to Korean peninsula and recently thousands of wild soybeans collected by soybean researchers in Korea. The objective of this study were to determine the linolenic acid content for wild soybean collection and to determine the stability of linolenic acid content derived from wild soybean over environments. Fatty acid profile for 1,806 wild soybean accessions collected from South Korea was determined by GC. The range of linolenic acid was 7.3 to 23.7% with an average 15.6%. We developed a recombinant inbred population from a cross PI483463 (wild soybean with 15% 18:3) and Hutcheson (cultivar with 8% 18:3). Three RILs, RIL156, RIL159 and RIL166, with high linolenic acid content (over 14%), parents and Williams 82 as checks were grown in nine environments over 2008-2011. Results showed that the content of linolenic acid for the PI483463, Hutcheson, and Williams 82 ranged from 14.8 to 17.1, 8.5 to 9.7, and 6.9 to 8.4 % and averaged 15.4, 9.2 and 8.0%, respectively. However selected RILs 156, 159, and 166 ranged from 10.7 to 15.7, 14 to 15.8, and 14.8 to 15.8, and averaged 13.9, 14.9, and 15.2, respectively. Among the tested accessions, RIL166 was the most stable with the lowest range and CV, and had a relatively lower stability coefficient value than other genotypes. Genes related to high linolenic acid from wild soybean may be useful in developing higher linolenic acid soybean genotypes and would broaden the use of soybean in food applications to improve human nutrition and health.

Soybean is desirable as a forage crop because of it has high protein and oil concentration. Wild soybean, a progenitor of cultivated soybean, has a softer stem and higher protein content in seed than cultivated soybean. There is little information on yield and forage quality for wild soybean and its derivatives. The objective of this study was to determine the forage yield and quality of wild soybeans and selected soybeans derived from a cross G. max ×G. soja. Forage yield and quality were assessed for three grain soybean cultivars, three wild soybeans and three selected lines from G. max×G. soja. Forage quality attributes such as crude protein (CP), crude fat (CF), neutral detergent fiber (NDF), acid detergent fiber (ADF), digestible dry matter (DDM), dry matter intake (DMI) and relative feed value (RFV) were determined at the R2, R4 and R6 developmental stages. Forage yield and CF were highest at stage R6 in G. max, G. soja and selected G. max×G. soja lines. CP content was similar between R2 and R4 but increased sharply after R4 and peaked at R6 in G. max and selected lines from G. soja×G. max. On the other hand, CP content was similar between R4 and R6 stage in wild soybeans. Generally, NDF and ADF were highest at stage R4 but decreased at stage R6. DDM, DMI, and RFV increased between R4 and R6. These results suggest that R6 was the optimal harvest stage to provide forage of highest quality and yield. A study was conducted in 2011 to evaluate forage yield and quality at stage R6 in 25 lines from PI483463 (G. soja)×Hutcheson (G. max) and four cultivated grain soybeans. Hutcheson had the highest forage yield with 24.7t/ha infresh weight (FW) among grain soybeans. Line W11 had the highest forage yield(25.7t/ha,FW) among G. soja×G. max selections and four other lines had similar forage yield compared to Hutcheson. Generally the 25 lines from this G. max×G. soja cross had thinner main stems and branches than cultivated soybeans. When the 25 lines were evaluated for their feed quality as per forage grade by AFGC, nine lines rated prime grade and all 25 lines were classified as forage Grade 1. Results of this study indicate crosses between wild and cultivated soybean show promise for improving soybean as a forage crop.

The main nutritional problem of sorghumxsudangrass hybrid (Sorghum bicolorxSorghum sudanese) silage is low quality and palatability. This experiment was conducted to evaluate whether organic com grain and crushed rice addition of sorghumxsudangrass hybrid silage increases forage quality of the silage. The sorghumxsudangrass hybrid silages with added com grain and crushed rice were similar to conventional corn silage in moisture content. However organic sorghumxsudangrass hybrid silage had low pH values. Silage added with gain and byproduct had higher crude ash, acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents than control corn silages, while its non-fiber carbohydrate (NFC) and total digestible nutrients (TDN) showed the opposite results. Lactic acid, butyric acid and lactic percentage oftotal organic acid (UT) of sorghumxsudangrass hybrid silages were higher than those of com silage, but acetic acid was higher than control. In vitro dry matter digestibi/ity (IVDMD) of com silage was higher than other sorghum silages. Feed intake of sorghumxsudangrass hybrid silage added with crushed rice was highest among silages. Therefore, these data indicate that crushed rice and com grain added sorghumxsudangrass hybrid silage could be recommended as the most effective treatment for increasing silage quality and palatability.