The purpose of this study was performed to determine the whitening effect of organic solvent extracts from the centipede, Scolopendra subspinipes mutilans. We prepared different concentrations (50%, 70% and 100%) of ethanol, methanol, 100% ethyl acetate and water extracts. We tested melanin inhibitory effect and tyrosinase activity using B16/F10 melanoma cell. As a result, treatment of organic solvent extracts is decreased the biosynthesis of melanin and tyrosinase activity to 30~60%. Especially the 70% ethanol extracts was the most effective in B16/F10 melanoma cells. In the study on melanogenic protein expression, 70% ethanol extracts of Scolopendra subspinipes mutilans blocked glycosylation of tyrosinase. Therefore this result suggests that 70% ethanol extracts could be developed as a skin whitening agents.

Previously, we have performed de novo RNA sequencing of Scolpendra subpinipes mutilans using next generation sequencing technology and identified several AMP candidates. Among them, a synthetic peptide (scolopendrasin I) was designed based on SVM algorithm. In this study, we reported that the synthetic peptide scolopendrasin I had an antimicrobial and anticancer activity. As a result, scolopendrasin I showed antibacterial activities against Gram positive and Gram negative bacteria strains in radial diffusion assay and colony count assay without hemolytic activity. In addition, we confirmed that scolopendrasin I bound to the surface of bacteria via a specific interaction with lipoteichoic acid and lipopolysaccharide, which is one of bacteria cell membrane components. In addition, we found that scolopendrasin I had anticancer activities in the human leukemic T lymphocyte cell line Jurkat using MTS assay. In conclusion, our results suggested that scolopendrasin I could be useful for developing peptide antibiotics and anticancer agents.

We estimate the power spectra of the cosmic microwave background radiation (CMB) temperature anisotropy in localized regions of the sky using the Wilkinson Microwave Anisotropy Probe (WMAP) 7-year data. We find that the north and south hat regions at high Galactic latitude (|b| ≥ 30°) show an anomaly in the power spectrum amplitude around the third peak, which is statistically significant up to 3. We try to explain the cause of the observed anomaly by analyzing the low Galactic latitude (|b| < 30°) regions where the galaxy contamination is expected to be stronger, and the regions weakly or strongly dominated byWMAP instrument noise. We also consider the possible effect of unresolved radio point sources. We find another but less statistically significant anomaly in the low Galactic latitude north and south regions whose behavior is opposite to the one at high latitude. Our analysis shows that the observed north-south anomaly at high latitude becomes weaker on regions with high number of observations (weak instrument noise), suggesting that the anomaly is significant at sky regions that are dominated by the WMAP instrument noise. We have checked that the observed north-south anomaly has weak dependences on the bin-width used in the power spectrum estimation, and on the Galactic latitude cut. We also discuss the possibility that the detected anomaly may hinge on the particular choice of the multipole bin around the third peak. We anticipate that the issue of whether or not the anomaly is intrinsic one or due to WMAP instrument noise will be resolved by the forthcoming Planck data.

A cell formation approach based on cluster analysis is developed for the configuration of manufacturing cells. Cell formation, which is to group machines and parts into machine cells and the associated part families, is implemented to add the flexibility and efficiency to manufacturing systems. In order to develop an efficient clustering procedure, this paper proposes a cluster analysis-based approach developed by incorporating and modifying two cluster analysis methods, a hierarchical clustering and a non-hierarchical clustering method. The objective of the proposed approach is to minimize intercellular movements and maximize the machine utilization within clusters. The proposed approach is tested on the cell formation problems and is compared with other well-known methodologies available in the literature. The result shows that the proposed approach is efficient enough to yield a good quality solution no matter what the difficulty of data sets is, ill or well-structured.

꼬마배나무이 (Cacopsylla pyricola)에 저항성 품종육성을 위한 육종재료를 선발코자 월동형 성충수, 단과지내의 산란수, 과총엽내의약충수 및 수관 전체적인 그을음 발생정도를 15개 종 및 종간잡종133개 유전자원을 대상으로 조사하였다. 월동형 성충수는 Pyrus.calleryana 4.6마리, 단과지내 산란수는 P. calleryana 0.3개, 과총엽내약충수는 P. calleryana 0마리, 그리고 그을음 발생정도는 P.betulaefolia, P. calleryana, P. communis, P. hybrid (P.pyrifolia × P.communis), P. lindleyi 0으로 발생 및 피해정도가 가장 낮았으며 종간의 차이가 뚜렷하였다. 전체적으로 대목으로 이용하고 있는 콩배인P. calleryana와 P. betulaefolia가 조사 대상 유전자원 중 가장 높은 항객성(antixenosis)을 보였으나 품질개량에 많은 기간이 요구됨에 따라 실용적으로는 서양배(P. communis) 중 ‘Conference', 'Cascade','Bosc', 'Winter Nelis' 가 가장 낮은 발생 및 피해정도를 보여 꼬마배나무이 저항성 품종 육성을 위한 좋은 육종재료로 판단되었다.

Genetic resources of buffalo, cattle, goat, sheep, deer, rabbit, pig, chicken, duck, goose, turkey and swan in Taiwan are conserved in living form or frozen genetic materials. Preserving farm animals in living form must conserve a considerable number of mature individuals with reproductive potential, and continued funding and breeding space are necessary. Based on the afore‐mentioned restraints and consideration of risk diversification, living animals are preserved in Taiwan Livestock Research Institute (TLRI) branches and breeding stock reproduction grounds throughout Taiwan. Genetic materials preserved in frozen form include germ cell (sperm, egg and embryo), tissue, somatic cell, cell line, DNA and gene pool. Those preserved for long term more than 10 years are conserved in liquid nitrogen storage tank at ‒185 to ‒196℃. Animal industry on utilization of farm animal genetics emphasizes the importance of in situ conservation and considers ex situ conservation as an essential complementary activity to in situ. In situ and ex situ utilization are complementary, not mutually exclusive. The exact strategy of germplasm cryobanking will clearly depend on the conservation objectives of TLRI. In situ and ex situ strategies differ in their capacity to achieve the different bio‐utilization objectives. Based on the objectives for bio‐utilization and conservation, the existing national technical capacity and infrastructure for cryoconservation, and amount of capital to invest in developing and maintaining a gene bank for food animal genetic resources (FAnGR), each country should determine for which FAnGR, if any, national cryobanking program should be undertaken. The health and sanitary issues of animals that must be considered when establishing and operating gene banks for animal genetic resources to help prevent the conservation of potentially dangerous pathogens along with the valuable genetic material in the ultra‐low temperature cell repository. Bio‐utilization of material stored in the bio‐bank must eventually be thawed and used to create new animals and therefore, national organization and annotation of the stored material is critical to ensure its proper utilization. Stock animals are the source of semen, embryo and oocytes are usually privately owned. This ownership may or may not change during the gene banking process, but the terms of agreements between bio‐banks and donors must be explicitly defined. Taiwan Animal Germplasm Center now conserves genetic resources of 77 livestock breeds and strains, including 19 native and 38 foreign species as well as 20 new breeds produced by inbreeding in poultry or breed‐crossing with the artificial insemination in livestock. Cryobanking of semen of native animals is for genetic diversity but also for risk management to long‐term global food security. Ex situ conservation program involving in vitro storage of germplasm cryobanking can contribute to ensure that it will be available to allow livestock keepers and animal breeders to confront future changes in animal production and economic environments.

This study was estimated for cabbage aphid, Brevicoryne brassicae in Brassica campestris L. var. rapa (L.) Hartm. in order to institute of Economic injury levels(EILs). B. brassicae was innoculated on April 29, in differently 0, 5, 10, 20 and 40 adults per ten plant, respectively. After inoculated of B. brassicae, the density was increased until harvest ing gradually in all plots except non innoculate plot. and Higher inoculation density were increased higher than lower inoculation density. Percentage of damage leaf was higher in plots with higher initial aphid density than in plots with lower initial aphid density. And the leaf weight of commodity were decreased in higher initial aphid density. The decreasing rates of leaf weight of commodity was increased with increasing initial aphid density. The relationship between initial B. brassicae densities and the decreasing rates of leaf weight of commodity was well described by a linear regression, Y=0.8416X-3.5147, R2=0.94. Based on the relationship, the number of adults per 10 plant which can cause 5% loss of yield was estimated to be approximately 10.1. And EILs was estimated to be approximately 1.0 adults/plant in late April.

Field survey were conducted in to evaluate the insect damage to growing Lemonbalm(Melissa officinalis). Lemaonbalm, belonging to the Lamiaceae family, is a smelling perennial shurb which is possible to grow in Korea. To date, there has been minimal research regarding plant culture, pest insect occurrence and management research about herbal plants in Korea. Meanwhile, it grows for processed food and cosmetics in Jellabukdo, and necessity to pest management is on the rise. Chrysolina exanthematica(Coleoptera: Chrysomelidae) was realized not pest but general insect in Korea. But, in this survey, it is found to pest about lemonbalm. Damage period was concentrated in April. Rest period was decreased in density. At size of Chrysolina exanthematica, larva is 7.0*3.6mm, pupa is 8.3*5.3mm, and adult is 9.7*6.7mm. In farm surveying about Chrysolina exanthematica host preference of 5 species herb plants, Lemonbalm has great preference but, Oregano has no preference. Surveying leaf damage about 6 species in Lamiaceae family in lab., Lemonbalm belonging to Melissa genus has great damage, Some species in Mentha genus have little damage. And, Oregano in Origanum genus no damage. In Growth characteristics of healthy and damage plant, plant length on damage plant is decreased 41.3%, number of tiller is decreased 67%. Fresh and dry plant weight of damaged plant is decreased 66.7%, 64.2% compared with healthy plant. Because damage of insect can influence on plant main contitutents, we analysed the constitutent parts of extracts from supercritical fluid extraction system using gas chromatography/mass spectrometer.

To control pest of Brassicaceae leafy vegetable(leaf broccoli, Red Mustard Leaf, Tatsoi) which grows at vinyl house in IKSAN, Jeollabuk-do, the non-treatment was set as negative control, and treatment was divided into Parasitic natural treatment group and general treatment group. And incidence density of pest was surveyed and control effect was analyzed. As a result, in case of spring plants, the leaf damage ratio was decreased by 31% in Leaf broccoli, 30% in Red mustard leaf and 27% in Tatsoi compared to untreatment. In case of autumn plants, it was decreased by 32%, 41% and 17% respectively. The key pests were Plutella xylostella, Pieris rapae, Myus persicae Sulzer, Thrips palmi and Striped cabbage flea-beetle. Compared with the untreatment, the incidence density of each was significantly controlled. Other pests include Spodoptera exigua, Macdunnoughia purissima, Macdunnoughia purissima which showed high incidental density sometimes. In case of spring plants, the number by treatment was increased by 117% in Leaf broccoli, 85% in Red mustard leaf and 1,000% in Tatsoi. In autumn plants, it was increased by 132%, 257% and 1,077% respectively. The used Parasitic natural and eco-friendly materials were Cotesia glomerata, lacewing, ladyburg, Orius laevigatus, Encarsia formosa, Entomopathogenic nematode, Chungjigi and Togkaki. During early development of each pest, the Parasitic naturals were grazed 2~3 times at the interval of 7~10days. During the peak time, eco-friendly materials were sprayed 1~2 times. Based on this, comprehensive management model was drafted by period for each Brassicaceae vegetables pest.

To control Thrips tabaci in Korean leek and green onion which grow at vinyl house in IKSAN, Jeollabuk-do, the non-treatment was set as negative control. In general treatment group, 120 mesh gauze was installed on the side window after planting(May, 2nd) and, in spring, Orius strigicollis (1.0/m2) was grazed 3 times every 7 days from mid of May which is early development stage and In the mid of June, which is the peak stage, plant extracts were sprayed one time. In autumn, O. strigicollis was grazed 3 times every 7 days from mid of September which is early development stage, and in the mid of October, which is the peak stage, plant extracts were sprayed one time. The result shows that the leaf damage ratio was decreased by 22% in Korean leek and by 27% in green onion compared to the untreatment. And the control value of Thrips tabaci shows 78.7% in korean leak and 90.6% in green onion. The density control effect of Thrips tabaci was significantly controlled under max 6.2/plant in general treatment group compared max 25/plant in the untreatment and this result was similar in green onion. The yields by general treatment was increased by 85% in Korean leek and 56% in green onion, compared with non-treatment yields, which was 900kg/10a in Korean leek and 1,287kg/10a in green onion.

This study was conducted to develop the economic threshold for the diamondback moth (Plutella xylostella (L.) on Leaf Broccoli (leaf vegetable) in 2007. To investigate the relationship between initial density of diamondback moth larvae and broccoli leaf yield, experimental plots with five treatments (0, 0.5, 1.0, 2.0, 4.0 larvae per plant) as initial density were established. We inoculated larvae in chesses cloth covering to survey larvae density change. When grown to eat, leaves of broccoli were harvested by periods. 60-70% of larvae were removed whenever we picked the leaves. High levels of larvae were associated with significant reductions in leaf yield. There were 85%, 64%, 58%, and 56% yield reductions from the diamond back moth larvae density in 0, 0.5, 1,0, 1.5, 2.0 per plant, respectively 25days after larvae inoculation. The regression equation used to predict leaf yield based on the number of initial larvae density per a plant was y=1635-393x(R2=0.79***). The economic injury level of diamondback moth on leaf broccoli was 2-3 larvae per 10 plants for a damage level of 5%. The economic thresholds was 1-2 larvae per 10 plants. Thus, the diamond back moth management should be initiated 1-2 larvae occurrence per plant.

This study was done to evaluate the susceptibility, systemic effect, residual effect and control effect in each developmental stages of biotype Q of sweetpotato whitefly against insecticides, acetamiprid+spinetoram SC and dinotefuran SG. Two insecticides were showed similar activity against the eggs, and showed higher activity in acetamiprid+spinetoram SC against the nymph and adult. In systemic effect, two insecticides have a similar activity. It was showed higher activity in root zone systemic application than leaf zone systemic application. Residual effect was showed higher in acetamiprid+spinetoram SC (92%) than dinotefuran SG (44 %) at seven days after treatment. Control effect was showed all over 90 % activity at tree- and seven days after treatment. Therefore, these insecticides are expected to control the sweetpotato whitefly effectively.