The insecticidal activity of cinnamon essential oils, cinnamon bark, cinnamon technical, cinnamon green leaf oils and their constituents and structurally related compounds against citrus flatid planthopper, Metcalfa pruinosa, was examined using a direct contact application. At 500 mg litre-1 very strong mortality (100%) was observed in cinnamon technical, cinnamon bark, cinnamon green leaf, mortality against flatid planthopper M. pruinosa. The cinnamon oils constituents were identified by GC-MS. The active principles were determined to be hydrocinnamic acid (24 h LC50, 30.66 mg/L), geranic acid (24 h LC50, 31.23 mg/L), cinnamaldehyde (24 h LC50, 32.65 mg/L), hydrocinnamaldehyde (24 h LC50, 39.11 mg/L) and trans-cinnamaldehyede (24 h LC50, 39.54 mg/L) were the most toxic against both nymph and adult of citrus flatid planthopper, M. pruinosa. The moderate activity was observed with cinnamyl acetate, dibutyl pthalate, anethole, α -cyano cinnamic acid, cinnamyl alcohol, methyl cinnamaldehyde, eugenol, 2.4-dihydrocyl cinnamic acid, bornyl acetate (24 h LC50, 53.35- 97.17 mg/L) respectively. The other constituents were showed less or no activity against adult of M. pruinosa. Global efforts to reduce the level of highly toxic synthetic insecticides in the agricultural environment justify further studies on the active cinnamon oils active principles act as potential insecticides for the control of M. pruinosa populations as direct spray with contact action.

Field survey were conducted in to evaluate the insect damage to growing Lemonbalm(Melissa officinalis). Lemaonbalm, belonging to the Lamiaceae family, is a smelling perennial shurb which is possible to grow in Korea. To date, there has been minimal research regarding plant culture, pest insect occurrence and management research about herbal plants in Korea. Meanwhile, it grows for processed food and cosmetics in Jellabukdo, and necessity to pest management is on the rise. Chrysolina exanthematica(Coleoptera: Chrysomelidae) was realized not pest but general insect in Korea. But, in this survey, it is found to pest about lemonbalm. Damage period was concentrated in April. Rest period was decreased in density. At size of Chrysolina exanthematica, larva is 7.0*3.6mm, pupa is 8.3*5.3mm, and adult is 9.7*6.7mm. In farm surveying about Chrysolina exanthematica host preference of 5 species herb plants, Lemonbalm has great preference but, Oregano has no preference. Surveying leaf damage about 6 species in Lamiaceae family in lab., Lemonbalm belonging to Melissa genus has great damage, Some species in Mentha genus have little damage. And, Oregano in Origanum genus no damage. In Growth characteristics of healthy and damage plant, plant length on damage plant is decreased 41.3%, number of tiller is decreased 67%. Fresh and dry plant weight of damaged plant is decreased 66.7%, 64.2% compared with healthy plant. Because damage of insect can influence on plant main contitutents, we analysed the constitutent parts of extracts from supercritical fluid extraction system using gas chromatography/mass spectrometer.

Previous studies indicated that Matsumuraeses phaseoli and M. falcana (Lepidoptera: Tortricidae) are separate species since a few differences were observed in genitalia morphology and female sex pheromone composition. A clear difference was detected in the DNA sequences of cytochrome oxidase I of the two species separately collected in different plants and regions. A hybridization test also showed that a post-zygotic reproductive isolation occurred between the species. In field monitoring, however, both species have been caught simultaneously and together in the separate sex pheromone traps installed for the two species around neighboring soybean and red bean fields. Molecular marker-assisted identification with several adults sampled from the trapped insects showed that only ca. 40% of M. phaseoli adults identified as the species by genitalia morphology was the M. phaseoli, while ca. 97% of M. falcana adults identified as the species was the M. falcana. The result indicated that the observation of genitalia did not make a decisive criterion for classification of the insects. Conclusively, it suggested that the sex pheromones of the two species should be studied more precisely although there is a possibility that the two species are hybridized in fields as in laboratory, and speciation is under process.

Matsumuraeses phaseoli and M. falcana cannot be classified with the external morphological characters. Although differences in the morphology of male genitalia and in the mitochondrial DNA sequence of cytochrome oxidase I between the two species have been found, there is no information that the two species are biologically different species till now. We, therefore in this study, tried to cross the two species to observe postzygotic incompatibility in the next hybrid generations and to know whether the two species are 'biological species' or not. In crossing between the parents, two kinds of F1 hybrids were produced successfully. In inter- and intra-breeding between F1 hybrids, two lines crossed with females of F1 hybrid produced by females of M. phaseoli could not produce F2 adults to show inviability of larvae. The other two lines produced F2 adults successfully and the F2 adults produced F3 larvae in inbreeding. In back-crossing between parents and F1 hybrids, the two lines of 8 breeding lines, in which females of F1 hybrid were produced by females of M. phaseoli, could not produce the next generation of larvae. The other six lines produced F2 adults successfully. The results indicated that maternal factors of F1 hybrid produced by M. phaseoli females contributed to create the incompatibility between the F1 females and other lines. In conclusion, the results showed a postzygotic reproductive isolation between M. phaseoli and M. falcana in part.

(E,E)-8,10-dodecadienyl acetate (E8E10-12:Ac) and (E)-8-dodecaenyl acetate (E8-12:Ac) have been selected as the candidate chemicals for sex pheromone components of the M. phaseoli, female through GC-EAD tests, whereas the two compounds and an additional candidate, (E,Z)-7,9-dodecadienyl acetate (E7Z9-12:Ac), have been found at a ratio of 7:1:1 in the abdominal tip extract (Yum et al., 2008). In order to determine the actual composition of sex pheromone, therefore, several blends using the three chemicals were evaluated for attractiveness to males of M. phaseoli around red bean and soybean fields. Individual components as well as two blends consisted of E8E10-12:Ac/E7Z9-12:Ac and E8-12:Ac/E7Z9-12:Ac did not show attractiveness, whereas the blend of E8E10-12:Ac/E8-12:Ac showed an increased effect in male capture. Of the tested blends with all three chemicals, the 7:1:2 composition of E8E10-12:Ac, E8-12:Ac and E7Z9-12:Ac attracted the most number of males. The results suggested that E7Z9-12:Ac is one of the sex pheromone components and may act as a synergist.

본 연구는 벼 품종에 따른 미음 가공특성을 구명하기 위하여 국립식량과학원에서 육성한 9개 벼 품종의 호화점도 특성과 미음의 색도, pH, 점도 및 퍼짐성을 측정하고, 초미세구조를 관찰하였다. 신속점도계를 이용한 호화 점도(페이스팅) 분석에서, 치반점도는 한아름4호, 화선찰이 각각 -45.84 및 -27.50 RVU, 최종점도는 화선찰, 한아름4호가 각각 109.00, 213.42 RVU로 미음죽의 노화가 다른 품종들에 비해 느릴 것으로 판단된다. 쌀 낟알과 미음의 미세구조를 주사현미경(SEM)으로 관찰하였는데, 쌀알은 다각형의 전분립으로 구성된 구 형태의 전분체와 이러한 전분체로 구성된 전분세포로 구성되어 있었다. 도담쌀은 구 모양의 전분체가 관찰되었고 다른 품종들에 비해 빈 공간이 더 많았으며, 한아름4호는 일반 멥쌀의 형태와 같은 다각형의 전분립을 확인하였고, 화선찰벼는 전분체를 구성하는 전분립들의 부서짐이 많은 것을 관찰하였다. 그리고 이 품종들로 만든 미음죽 호화양상은 품종별로 달랐는데 이는 주로 아밀로오스 함량 차이에 기인하는 것으로 판단되나, 같은 고아밀로오스 품종이라 하더라도 도담쌀에 비해 고아미4호는 잘 풀어지고 호화가 잘 되는 것으로 나타나는 바(data not shown), 이에 대한 추가적인 연구가 필요하다 하겠다. 품종별 미음 제조 후, 관능검사에서 통일형(초다수성) 벼품종과 자포니카 벼 품종들의 차이가 뚜렷하게 나타나지 않아 초다수성 벼 품종으로 자포니카 벼 품종을 대체할 수 있을 것으로 판단하였다(data not shown). 또한 아밀로오스 함량 차이에 따른 벼 품종들 간의 점도 차이가 확연하게 나타났는데 아밀로오스가 없는 찰벼 품종은 미음죽의 점도를 낮추고, 고아밀로오스 품종들은 미음죽의 점도를 높였다. 통일형(초다수성 벼)을 포함한 일반메벼 품종들의 점도는 찹쌀과 고아밀로오스 품종들로 만든 미음의 중간정도를 보였으며 품종간의 유의한 차이는 없었다. 요컨대, 통일형 품종은 일반쌀 품종에 비해 밥용으로 식미가 나쁜 것으로 알려져 있으나, 미음에 대한 가공적성 평가 결과, 통일형쌀 품종과 일반쌀 품종 간에 품질 차이가 없었다. 쌀미음의 점도와 퍼짐성은 쌀미음의 품질을 결정하는 중요한 요소이며 9개 품종(통일형 4개 품종, 일반형 5개)의 쌀미음 가공적성을 평가한 결과, 고아밀로오스(31.8%-42.8%) 쌀 품종들은 점도가 높아(낮은 퍼짐성) 걸쭉하였고, 저아밀로오스(5.0%)인 찹쌀 품종은 낮은 점도(높은 퍼짐성)로 유동적이었다. 중간정도의 특성을 가진 멥쌀(18.2%-19.9%) 품종들이 쌀미음에 적합한 것으로 나타났다. 멥쌀 품종들 중에서 생태형(통일형/일반형)에 따른 쌀미음의 점도와 퍼짐성에서 차이가 없었다.

Background : The study was conducted in response to climate change, to develop cultivation techniques by introducing a new crop income. Therefore, we want to collect the Coriander sativum genetic resources that are grown in different regions of the korea and other countries. The C. sativum were strengthens the stomach to say "housil" in Herbal and prosper digestion. Recently, several nations, including Vietnam and Thail and increased in Korea. C. sativum increased eating enjoy food. The people who like C. sativum in Korea is estimated to be 1.5 million. Methods and Result : First was C. sativum 93 species in National agrobiodiversity Center RDA lotting-out on May 12, 2016 for the study. C. sativum germplasm received lotting-out of over thousand grain 15g 28 kinds of seeds, 10∼15g was 23 species, 10g less than 42 species. 32 kinds of fruit color is white, black, 30 species, the gray was 31 species. Conclusion : C. sativum lotting-out was conducted by resource for two weeks from germination test from May 31 to June 13. The results were as follows: C. sativum port germination 86∼100% germination was 43 stocks, 40∼60% of 23 stocks, 5 stocks of 20∼ 40%. The 22 stocks were not germinate paper. C. sativum resources per port germination test was conducted 13 days from 29 days July until August 9th. As a result, more than 90% of the port germination is 62 stocks, not more than 80∼90%, 50∼80% 18 stocks of germination was 5 stocks. At this time, no germination was 8 stocks. Therefore, C. sativum germination test is a port germination test obtained a more than satisfactory result.

Background : Amaranthus is a cosmopolitan genus of annual or short-lived perennial plants. Some amaranth species are cultivated as leaf vegetables, pseudocereals, and ornamental plants. Most of the species from Amaranthus are summer annual weeds and are commonly referred to as pigweed. Approximately 60 species are recognized, with inflorescences and foliage ranging from purple and red to green or gold. Members of this genus share many characteristics and uses with members of the closely related genus Celosia. Amaranthus shows a wide variety of morphological diversity among and even within certain species. This study was conducted to evaluate the growth characteristics and grain yield of Amaranthus under subalpine area(altitude 510m) Methods and Results : Six resources(GR-1∼6) were tested in this experiment. Its seedlings were sown at 21. March. and raised for 27 days in nursery bed. The days after sowing for emerging 80% seedlings was 14∼18 days and No. of leaves was 4.5∼5.2 ea./plant. Plant height of GR-4 was highest at 15.2㎝ in nursery period. These seedlings transplanted at 19. April in the planting density of 80×30㎝. The range of heading date was 25. May∼7. June. GR-1, 2 showed more early heading at 25. May but heading date of GR-6 was 7. June. The Flowering date of Amaranthus cultivated in subalpine area was 20∼28. June. Plant height at flowering time of GR-4 was significantly high at 152.7㎝ comparison with 95.2㎝ of GR-1 but No. of branches in GR-4 was lowest at 7.5 ea/plant. The length of ears was shortest 28.8 ㎝ in GR-5 compared 41.0㎝ of GR-4. Grain yield of Amaranthus harvested at 8. July showed significantly difference according to gene resources and the highest yield of grain was 556.8㎏ in GR-1 Conclusion : The growth and ripening characteristics of Amaranthus tricolor showed many difference under subalpine cultivation according to gene resources. The predictable grain yield of Amaranthus under subalpine cultivation was 240.0∼556.8㎏/10a according to gene resources and their cultivation period demanded 80 days.

Background : This study was conducted to developed the propagation method by cutting for mass cultivation of Vitex roundifolia. We were pitched the cutting two times and treated plant growth regulators to enhance the rooting percentage. Vitex roundifolia is live in beach sandy soil south of Hwanghae-Do and Gangwon-Do. Vitex roundifolia have been used to bath foam. It is good for aromatic plant. It has 0.8% essential oil content in leaf and flower. Major components of essential oil were alpha-Pinene, Sabinene, beta-Pinen, 1,8-cineole, d-Limone. Despite the superior usability, it had not yet been made by the artificial cultivation Methods and Results : We were pitched the cutting of a first-year branch on June 5, which was greenwood cutting and July 17, which was semi-hardwood cutting at Kwangseung-ri beach, Gochanggun, Jeonbuk. The length of cutting was 10cm. It had 3～4 nodes, we stuck a cutting remain 2 nodes above ground on ordinary raise seedling soil. Rooting percentage was measured at 60 days after stuck a cutting. Rooting percentage was higher greenwoody cutting(95%) than semi-hardwood cutting(57.6%). In green wood cutting, there was no significance with plant growth, but chemical injury was occurred in IBA 5,000ppm. In semi-hardwood cutting, there was significance with plant growth regulators. The rooting percentages of all the treat were higher than control(no treatment). Rooting percentage was the highest in NAA 5,000ppm treated. Conclusion : Greenwood cutting method was more proper to propagation for Vitex roundifolia than semi-hardwood cutting. The optimum time to cutting for Vitex roundifolia propagation was the early in June. If miss a time to propagation Rooting percent was elevated by plant growth regulator.

Background : This experiment was conducted to find the right degree of shading to reduce summer months when the death Allium hookeri cultivation in the southern highland in Korea. This experiment was conducted at an altitude of 500m, Jeonbuk Namwon unbong plateau region The soil was tested in phosphatic content was slightly higher so 671 ㎎/㎏ than regular soil. Every soil testing was conducted in sandy soil with good water. The test day highs at 50% shading plots of shading in this period about 6.1℃ lower than the non-shading. The soil temperature is lowered to 1.9℃ at 50% shading plots compared with the non-shading. As a result, Allium hookeri underground quantities produced per 1,682 ㎏ 10a in 50% shading plots. This was a 35% increase compared to the non-shading plots. Allium hookeri is native to the Alpine 4,200m from 1,400m above sea level, near the Himalaya Mountains. Myanmar is known as the National vegetables. Allium hookeri is Allium plants. It flies three flavors, including sweet, spicy, bitter taste. Allium hookeri roots are similar to ginseng roots called as 'three vegetable' This is a randomly named. The in Korea was first introduced in 2006. By 2015, the country has been found to 190㏊, Jeonbuk 35㏊ (18.3%) is growing. Therefore, this study was conducted to introduce the cultivation of plants in the southern highland in Korea. Methods and Results : In order to use this experimental Allium hookeri seedlings were planted in Jeonbuk Sunchang after buying. Allium hookeri cultivation methods put the 3∼4 one eye on the roots. Planting the root length was cut to leave a 3∼5㎝. The planting distance was 40×20㎝. The shielding 35, two months were 50 and 75% by July 1, August 31. The air temperature, soil temperature were investigated in accordance with the changes of light in this experiment. And examined the withering rate, brightness, growth conditions, such as Yield. Conclusion : Test results were as follows. Allium hookeri roots emerged on March 2 during planting 43-68 days. Allium hookeri after shading shoot growth characteristics were as follows. 35% shading and 50% shading was a tendency for the number of leaves increased compared to the non-shading. As a result, flooding was 35% as compared to production per 10a 1,682 ㎏ no light shielding at 50%. Plant analysis leaf mineral contents tended increased nitrogen, phosphate, gallium compared to the non-light cultivation. Mineral contents of the root was nitrogen, potassium is higher in comparison with 75% shading no shading.

Cryopreservation has been known as an efficient method for long-term preservation of clonally propagated plants, and several cryopreservation methods have been developed. Among them, a droplet-vitrification method for potato using axillary shoot tips in vitro has been established previously. In this study, we have optimized the procedure in which explants were submitted to a step-wise pre-culture in liquid sucrose-enriched medium (0.3 and 0.7 M for 7 and 17 h, respectively). The pre-cultured explants were dehydrated with PVS3 (w/v, 50% glycerol + 50% sucrose) for 90 min or modified PVS2 vitrification solution (w/v, 37.5% glycerol + 15% DMSO + 15.0% ethylene glycol + 22.5% sucrose) for 30 min. This two dehydration solutions produced post-cryopreservation regeneration percentages of 57.2% and 80.9%, respectively. We also compared a new post-culture medium (0.1 mg L ･ -1 GA3, 0.1 mg L ･ -1 kinetin) with the conventional one (0.15 mg L ･ -1 IAA, 0.2 mg L ･ -1 zeatin, 0.05 mg L ･ -1 GA3); the shooting initiation rates were 80.9% and 43.5%, respectively. The results suggest that the modified droplet-vitrification protocol described in this study is more effective, easier to implement, and more economical than the droplet-vitrification protocols currently used for potato.

"Chuyoung" as a new double cropping potato variety was bred in 2005 for table use through a cross between "Dejima" with short tuber dormancy and HRB-31 which is a tetraploid derived from an interspecific cross between "Russet Burbank" and Solanum phureja line. It was evaluated for short dormancy, growth and tuber characteristics every twice a year from 1997 to 2001. Regional yield trials were performed from 2002 to 2004 at three locations, Jeju, Namhae and Gangneung of Korea, respectively. Its tuber shape is oblong with yellow skin and flesh colors. Its dormant periods is 60~70days. It showed less incidence of physiological disorders such as cracking or knobs on tubers, and higher resistance to common scab by Streptomyces scabies compared to "Dejima". It has tall plant height and erect growth type with green-broad leaflets and white large inflorescence. Its average yields are 29.5 and 22.6 ton/ha at 90 days after planting in spring and autumn cropping, respectively. It also showed higher marketable yield due to the lower incidence of common scab and physiological disorders compared to "Dejima".

"Chugang" as a new double cropping potato variety for table use was bred in 2003 from a cross between H83005-2 with short dormancy and "Superior" with common scab resistance. It had been evaluated for short dormancy, growth adaptability and tuber characteristics every twice a year from 1990 to 1999. Regional yield trials had been performed from 2000 to 2002 at three locations, Jeju, Namhae and Gangneung of Korea, respectively. It has round oblong tuber shape with yellow skin and white flesh color. Its tuber dormant periods is approximately 50~60 days. It showed less physiological disorders such as cracking or knobs, and more tolerant to environmental stresses such as plant death by flooding and damages by frost or severe wind compared to "Dejima". Average yields of "Chugang" are 34.6 and 31.1 ton/ha at 90 days after planting in spring and autumn cropping, respectively. It is more resistant to common scab and less susceptible to late blight (phythophthora infestans) compared to "Dejima". However, it is susceptible to potato virus Y (PVY) and andean mosaic virus (AMV).

Lately, the autumn cropping areas of potato have been steadily increased to supply fresh potatoes all year round in Korea. Although "Dejima" is a main cultivar for autumn cropping, it is very susceptible to common scab (Streptomyces scabies) and show high frequency of physiological disorders such as knobs and cracking on tubers. "Chudong" was bred in 2002 for fresh table use from a cross between H83520-3 with short tuber dormancy and "Superior" with common scab resistance. It has attractive, round tuber shape with white skin and flesh colors. Its tuber dormant periods is 5 days longer than 65 days of "Dejima". Its average yields were 32.4 and 21.8 ton/ha at 90 days after planting in spring and autumn cropping of regional yield trials, respectively. It is more resistant to common scab and potato leaf roll virus and less susceptible to late blight (Phythophthora infestans) compared to "Dejima". However, it is susceptible to potato virus Y.