We developed a type of sausage made of chicken breast and liquid egg whites for consumers interested in weight management. To determine the quality of the product, its chemical characteristics, fatty acid composition, free amino acid contents, and nucleotides contents were evaluated during 4 weeks of storage. Sensory evaluation was conducted by both general consumers and body-builders. The sausage was proposed as a fat-free product as fat content was 0.12% based on the Korean Indication Standard of Animal Origin Food. Protein content was 13.42% and calorie value was 61.50 kcal/100 g of the sausage. In sensory evaluation, the mixture of chicken breast and egg whites stuffed into the same casing had an adverse effect on taste, color, texture and overall acceptance while the product that contained egg white stuffed separately into the outer casing enclosing the chicken breast (double layer) improved these attributes. The developed double-layer sausage can last for at least 4 weeks of storage without quality deterioration of flavor-related compounds, such as fatty acids and nucleotides.

Even though klotho deficiency in mice exhibits multiple aging-like phenotypes, studies using large animal models such as pigs, which have many similarities to humans, have been limited due to the absence of cell lines or animal models. The objective of this study was to generate homozygous klotho knockout porcine cell lines and cloned embryos. A CRISPR sgRNA specific for the klotho gene was designed and sgRNA (targeting exon 3 of klotho) and Cas9 RNPs were transfected into porcine fibroblasts. The transfected fibroblasts were then used for single cell colony formation and 9 single cell–derived colonies were established. In a T7 endonuclease I mutation assay, 5 colonies (#3, #4, #5, #7 and #9) were confirmed as mutated. These 5 colonies were subsequently analyzed by deep sequencing for determination of homozygous mutated colonies and 4 (#3, #4, #5 and #9) from 5 colonies contained homozygous modifications. Somatic cell nuclear transfer was performed to generate homozygous klotho knockout cloned embryos by using one homozygous mutation colony (#9); the cleavage and blastocyst formation rates were 72.0% and 8.3%, respectively. Two cloned embryos derived from a homozygous klotho knockout cell line (#9) were subjected to deep sequencing and they showed the same mutation pattern as the donor cell line. In conclusion, we produced homozygous klotho knockout porcine embryos cloned from genome-edited porcine fibroblasts.

The squid Todarodes pacificus is a commercially important fishery species in East Asia. As East Asians consume raw or lightly cooked squids, there has been growing concern about parasitic infections associated with squid consumption. In the current study, five squids caught in the East Sea were sampled for parasitological research from the biggest fishery market in Seoul, Republic of Korea. They were dissected and examined for parasites using a light microscope. Proteocephalus sp. was isolated and identified using field emission scanning electron microscopy, PCR, and sequencing. Given that Proteocephalus spp. can be affected by water temperature and geographical characteristics, further research on Proteocephalus spp. with respect to changes in water temperature in the East Sea is important.

We study an association between the duration of solar activity and characteristics of the latitude distribution of sunspots by means of center-of-latitude (COL) of sunspots observed during the period from 1878 to 2008 spanning solar cycles 12 to 23. We first calculate COL by taking the area- weighted mean latitude of sunspots for each calendar month to determine the latitudinal distribution of COL of sunspots appearing in the long and short cycles separately. The data set for the long solar cycles consists of the solar cycles 12, 13, 14, 20, and 23. The short solar cycles include the solar cycles 15, 16, 17, 18, 19, 21, and 22. We then fit a double Gaussian function to compare properties of the latitudinal distribution resulting from the two data sets. Our main findings are as follows: (1) The main component of the double Gaussian function does not show any significant change in the central position and in the full-width-at-half-maximum (FWHM), except in the amplitude. They are all centered at  11◦ with FWHM of  5◦. (2) The secondary component of the double Gaussian function at higher latitudes seems to differ in that even though their width remains fixed at  4◦, their central position peaks at  22.1◦ for the short cycles and at  20.7◦ for the long cycles with quite small errors. (3) No significant correlation could be established between the duration of an individual cycle and the parameters of the double Gaussian. Finally, we conclude by briefly discussing the implications of these findings on the issue of the cycle 4 concerning a lost cycle.

In establishing and overseeing ASIAPARC Federation in March of 2016 in Jeju Island Korea, it will be essential for us to learn aspiration, network, purpose, values, goals and strategies from 42 years experiences of EUROPARC Federation as our partnership organization such as practicing exchange programs and joint conferences. We also believe we can add one more principle, ‘Initiating Island Resilience’, into the three principles of the Jeju Declaration of 1) Scaling up Conservation, 2) Nature-based Solutions, and 3) Sustainability in Action through the opening of a "Green Growth Organization" of the Jeju Declaration of WCC 2012.

Nutritive value analysis of feed is very important for the growth of livestock, and ensures the efficiency of feeds as well as economic status. However, general laboratory analyses require considerable time and high cost. Near-infrared reflectance spectroscopy (NIRS) is a spectroscopic technique used to analyze the nutritive values of seeds. It is very effective and less costly than the conventional method. The sample used in this study was a corn kernel and the partial least square regression method was used for evaluating nutrient composition, digestibility, and energy value based on the calibration equation. The evaluation methods employed were the coefficient of determination (R2) and the root mean squared error of prediction (RMSEP). The results showed the moisture content (R2val=0.97, RMSEP=0.109), crude protein content (R2val=0.94, RMSEP=0.212), neutral detergent fiber content (R2val=0.96, RMSEP=0.763), acid detergent fiber content (R2val=0.96, RMSEP=0.142), gross energy (R2val=0.82, RMSEP=23.249), in vitro dry matter digestibility (R2val=0.68, RMSEP=1.69), and metabolizable energy (approximately R2val >0.80). This study confirmed that the nutritive components of corn kernels can be predicted using near-infrared reflectance spectroscopy.

Islanders have tried to find out real alternatives on how to keep sustainable Haenyeo communities through both developing traditional school system at community level (Hansupul Haenyeos School) and advanced school system at university level ( an educational certificate program for Interpreter of Haenyeos Culture and an advanced certificate program for Hanyeos Culture). In oder to share their wisdom of ordinary democratic life style around Bultuk, We created Global Peace Bultuk Assembly for younger generation to succeed in their tradition in July of 2010. It' s a time for us to find out Integrating Policy of the Sustainability of Jeju Haenyeo (Jamnyeo, Jamsu) Community facing their extinction into Jeju Village Conservation Model of Marine Resources Needed.

Apolygus spinolae (Meyer-Dür) (Heteroptera: Miridae) is an important pest of fruit and tea trees in Korea and Japan. Analyses of extracts of metathoracic scent glands revealed that those of female bugs contained hexyl butyrate, (E)-2-hexenyl butyrate, and (E)-4-oxo-2-hexenal in a ratio of 20:100:7. The glands of males contained the same three compounds, but the ratio of the components was quite different, with hexyl butyrate being the most abundant. Field trapping tests with various blends of the synthetic compounds dispensed from high-density polyethylene tubes showed that (E)-2-hexenyl butyrate and (E)-4-oxo-2-hexenal were essential for attraction of male A. spinolae, and catches with a wide range of ratios of these two compounds did not differ significantly. However, adding hexyl butyrate at 50% or more of the (E)-2-hexenyl butyrate to the binary blend strongly inhibited attraction of males. Trap catches increased with increasing amounts of a 10:1 blend of (E)-2-hexenyl butyrate and (E)-4-oxo-2-hexenal from 0.011 to 11 mg loaded into the tube. Catches of males in traps baited with lures containing 1.1 mg of the binary blend were not significantly different from catches in traps baited with live virgin females.

We would like to present three aspects of Jeju global governance of World Heritages in Jeju Island. To accomplish its international designations, including the UNESCO triple crown, intangible cultural heritages, and RAMSAR wetlands sites, the Jeju Government has created a GIS system where 5 types of land-use have been successfully designated and managed for the preservation of the Environment: 1) Absolutely Preserved Zone, 2) Relatively Preserved Zone, 3) City Planning District, 4) Preserved Eco-System District, 5) Preserved Scenery District. Through these 5 land-use categories, we can see how as a whole, the land is managed, protected, and kept pristine.Jeju must embody the three goals of the Jeju Declaration: “Scaling up Conservation,” “Nature-based Solutions,” and “Sustainability in Action.” Jeju has the ability to mobilize communities to work for biodiversity conservation and sustainable development. Jeju also has the ability to halt biodiversity loss and apply nature-based solutions to conserve biodiversity, enhance resilience, and so improve the well-being of the people on the island and in turn to improve the well-being of the people on the planet. To actualize practices of 4 Jeju Motions (M067; Establishment of an Integrated Management System for UNESCO Protected Areas, M 108 : Supporting the Sustainability of Jeju Haenyeos as a Unique Marine Ecology Stewardship, M 162 : The Development of an Evaluation and Certification System for World Environment Hubs and unapproved motion, M 181 : Protection of the People, Nature, Culture and Heritage of Gangjeong Village), Jeju National University opens Peace Island Leadership School. We also need to involve in an initiativeto integrate each component into a framework of a World Environment University grounded in the Island province of Jeju in South Korea. Within that framework the establishment of a Green Growth and Travelism Institute is a priority element. As a torchbearer of the green growth 2050 vision, this is envisaged as the centre of a virtual global network of related organizations and institutions.