The genomic structure and phylogenetic relationships of HSP88 genes from P. tenuipes Jocheon-1, P. tenuipes, C. militaris and C. pruinosa are described. The HSP88 genomic DNA from P. tenuipes Jocheon-1, P. tenuipes and C. militaris all contain 5 introns and 6 exons with the length of 13, 62, 32, 1438, 306, 288 bp, encoding 713 amino acid residues. C. pruinosa HSP88 genomic DNA contains 4 introns and 5 exons encoding 713 amino acids. The length of each exon of C. pruinosa HSP88 is 13, 62, 32, 1744, 288 bp and the length of exon 4 is identical to the total length of exon 4 and exon 5 of HSP88 of P. tenuipes Jochoen-1, P. tenuipes, and C. militaris. The deduced amino acid sequence of P. tenuipes Jocheon-1 HSP88 showed 99% identity with the P. tenuipes, 97% identity with the Cordyceps militaris, and 98% identity with the C. pruinosa. Phylogenetic analysis confirmed that the P. tenuipes Jocheon-1, P. tenuipes, C. militaris and C. pruinosa HSP88 are placed together within the ascomycetes group of fungal clade.

In this study, a full-length heat shock protein88 complementary DNA (cDNA) of Paecilomyces tenuipes Jocheon-1 was obtained by screening of P. tenuipesJocheon-1 Uni-Zap cDNA library and 5' RACE polymerase chain reaction. The Paecilomyces tenuipes Jocheon-1 heat shock protein88 cDNA contains an open reading frame of 2,139 bp encoding 713 amino acid residues. The deduced amino acid sequence of the P. tenuipes Jocheon-1 HSP88 cDNA showed 77% identity to N. haematococca HSP88 and 45-76% identity to other fungi HSP88. Phylogenetic analysis and BLAST program analysis confirmed that the deduced amino acid sequences of the P. tenuipes Jocheon-1 HSP88 gene belonged to the ascomycetes group within the fungal clade and P. tenuipes Jocheon-1 HSP88 also contains the conserved ATPase domain at the N-terminal. The cDNA encoding P. tenuipes Jocheon-1 HSP88 was expressed as a 88 kDa polypeptide in baculovirus-infected insect Sf9 cells. Under different stress conditions, mRNA expression of P. tenuipes Jocheon-1 HSP88 were quantified by real-time PCR and the result showed that heat shock stress affected the mRNA expression levels of P. tenuipes Jocheon-1 HSP88.

The phase Ti-Nb-Sn-HA bio materials were successfully fabricated by high energy mechanical milling and pulse current activated sintering (PCAS). Ti-6Al-4V ELI alloy has been widely used as biomaterial. But the Al has been inducing Alzheimer disease and V is classified as toxic element. In this study, ultra fine sized Ti-Nb-Sn-HA powder was produced by high energy mechanical milling machine. The phase Ti-Nb-Sn-HA powders were obtained after 12hr milling from phase. And ultra fine grain sized Ti-Nb-Sn-HA composites could be fabricated using PCAS without grain growth. After sintering, the microstructures and phase-transformation of Ti-Nb-Sn-HA biomaterials were analyzed by scanning electron microscope (SEM) and X-ray diffraction (XRD). The relative density was obtained by Archimedes principle and the hardness was measured by Vickers hardness tester. The -Ti phase was obtained after 12h milling. As result of hardness and relative density, 12h milled Ti-Nb-Sn-HA composite has the highest values.

The main develop stages of forage breeding in China was reviewed. The achievements in forage germplasm collection, preservation and evaluation were briefed. Registration varieties were classified. The improvement of forage breeding methods and techniques were summarized. Finally, make prospects on forage breeding of China.

In this study, GSTs gene homology fragment from Mongolian wheatgrass(Agropyron mongolicum Keng) was isolated using homology-based method. one partials gene sequences have been obtained by reverse transcription polymerase chain reaction (RT-PCR). Sequence analysis using Software of DNAman and DNAuser etc, and showed that the cDNA sequences was 344 base pairs, encoding 62 amino acids. The partial gene had C-terminal conserved domains of substrate binding pocket (H-site) of GSTs superfamily. Homology comparison with GSTs gene amino acid sequences in other plants showed that it was 91% identical to 19E50 of wheat, 90% to pk0078 and 88% to gstf6. It was named as MwGSTs.

Thee kinds of explants, young spikes, immature and mature embryos, was compared in callus induction, embryogenic callus formation, differentiation and plant regeneration of Russian wildrye. Callus induction efficient of young spikes is highest. The imature embryos formed more somatic embryogenesis, and with highest frequency of embryogenic callus.

The cultivation of oat in China could be traced back to 2500 B.C. while the Qinghai province, which located in the North-East of the Qinghai-Tibetan Plateau, has been the major region for oat production due to its high quality caused by the unique climate say long sun-shine hours and large temperature discrepancy between days and nights that is beneficial to the accumulation of nutrients in oat plant. Oat has been grown in the Qinghai alpine region on the altitude ranging from 1600m to 3600m and regarded as an ideal forage plant due to its resistance to environment. The role of oat drew more attention in 1980s along with the implementing of so called four-matched policy, which compounds establishment of fenced rangeland, animal bars, fodder production base and settled living place for local herdsmen who used to be nomadic around the region. To make a full use of the oat resource in Qinghai province, a great deal research and extension with respect of oat had been engaged in the region. The author overviewed researches accomplished in Qinghai province on oat germplasm collection, breeding of fine oat variety, cultivate techniques, oat base establishment and production and marketing system establishment as well in terms of projects completed.

The question of conflict of jurisdictions between courts and arbitral tribunals not merely constitutes a purely theoretical issue, but it also has significance in the legal practice. When many countries show allowance to arbitration and support its development to the greatest extent, few courts in China fail to tackle the relationships between arbitration and courts. By means of an analysis of possible conflict of the jur isdictions between courts and arbitral tribunals, this article pleas for a supportive attitude of courts with respect to arbitration, so as to reduce the impact of courts’ judicial supervision on the due arbitration proceeding. In-depth theoretical discussions will develop on the principle of “competence-competence” and its application in China, justifying the conclusion that the essence of this principle is to have disputes between parties in arbitration agreements solved within the arbitration system and thereby to respect the parties’ decision to submit to arbitration.

Sprites, elves and blue jets are collectively denominated as the upper atmospheric transient luminous events (TLEs). They are recently discovered optical flashes between active thunderstorms and the ionosphere. In this report, a brief introduction to the most important characteristics of TLEs is given. Since 2001, scientists from the National Cheng Kung University have been performing yearly summer campaigns from various locations in Taiwan. The main achievements of their yearly campaign are presented.

This paper concerns with the relationship between the visual perception of the degree of pucker or wrinkles of garment surfaces and the geometrical parameters of surfaces. In this study, four potentially relevant parameters of the surface profile are considered, namely, the variance (σ2), the cutting frequency (Fc), the effective disparity curvature (Dce) (Defined as the average disparity curvature of the wrinkled surface over the eyeball distance of the observer) and the frequency component of the disparity curvature ( Dcf). Based on the experiments using garment seams having varying degree of pucker (i.e. the wrinkles along a seam line), it was found that, while the logarithm of each of these four parameters has a strong linear relationship with the visually perceived degree of wrinkles, following the Web-Fetchner Law, the effective disparity curvature ( Dce) and the frequency component of the disparity curvature (Dcf) appeared to have stronger relationships with the visual perception. This finding is in agreement with the suggestion by Rogers & Cagenello that human visual system may compute the disparity curvature in discriminating curved surfaces. It also suggested an objective method of measuring the degree of surface wrinkles.

With the rapid development of the Internet, mobile Internet, big data, cloud computing and other technologies as well as the continuous improvement of logistics infrastructure, cross-border e-commerce has been developing rapidly in China. As a close neighbor of South Korea, the development of crossborder e-commerce has given wings to the development of trade between China and South Korea. Shandong Province, as an eastern coastal city of China, has a unique advantage in trade with Korea. In this paper, we will study the current situation of trade development between Shandong and Korea, analyze its problems, and put forward corresponding countermeasures and suggestions.

China and South Korea, as countries belonging to the East Asian cultural circle, have always attached great importance to education service trade. Nowadays, economic diversification has become the trend of world development, education service trade has become an important factor affecting the comprehensive strength competition between countries. China and the ROK are close in geographical location and have profound cultural origins. Over the years, the two countries have conducted fruitful bilateral cultural exchanges. This paper takes China and South Korea as the research object, and the comparative study of Sino-South Korea education service trade is of great significance to promote the development of China’s cultural products and service trade. Enhancing the competitiveness of China’s trade in cultural products and services can further improve China’s cultural soft power, so as to improve the development strategy of service trade and enhance its international influence in the context of China’s economic globalization.

Today, with the deepening cooperation between China and South Korea on public service advertising, it is very necessary to conduct a comparative study on public service advertising between China and South Korea. Due to the cultural differences between China and South Korea, there are certain differences in the theme, appeal methods and audience acceptance psychology of public service advertisements. This paper makes a comparative analysis of the two countries’ TV public service advertisements, aiming to explore the different presentation of theme design, creativity, appeal performance and advertising effect in the two countries’ TV public service advertisements and analyze the influencing factors of the difference formation, as well as to analyze and discuss the development strategies of Chinese and Korean public service advertisements.