Recently, we constructed a novel recombinant baculovirus genome, bEasyBac, enabling easy and fast generation of pure recombinant baculovirus without any purification step. In the bEasyBac, bacteriophage lambda site-specific attachment (att) sites were introduced to facilitate the generation of recombinant viral genome by in vitro transposition. Moreover, extracellular RNase gene from Bacillus amyloliquefaciens, barnase, was expressed under the control of Cotesia plutellae bracovirus (CpBV) ORF3005 early promoter to negatively select against non-recombinant background. The bEasyBac could replicate in host insect cells only when the barnase gene was replaced to gene of interest by in vitro transposition. When the bEasyBac was transposed with pDualBac-EGFP and the EGFP expression efficiency along passage was investigated, the resulting recombinant virus, EasyBac-EGFP, showed comparable level of EGFP expression efficiency with the plaque-purified recombinant virus, AcEGFP, which was constructed using bAcGOZA system, whereas, the non-purified AcEGFP showed quite reduced level of EGFP along passages. Moreover, no non-recombinant backgrounds were detected from unpurified EasyBac-EGFP stocks. Based on these results, high-throughput condition for generation of multiple recombinant viruses in a time was established. These results suggest that the bEasyBac has an effective benefit enabling for high-throughput baculovirus expression vector without purifying recombinant virus.

Among the cultured products of Beauveria bassiana SFB-205 (KCCM 10892P), the supernatant showed the highest insecticidal activity against 2nd instars of Aphis gossypii (Aphididae) nymphs under glasshouse condition. The enzymes in the supernatant were confirmed as active materials, and the chitinase was finally selected as a QC factor for commercial production. However, the chitinase activity in the supernatant decreased by 11-folds due to the thermal stress at 50℃ for 2 h. To obtain thermal stability, the chitinase in the supernatant was adsorbed to a precipitable material and the pellet was freeze-dried (PCT/KR2007/005886). The adsorbent-A showed about 92.7% of harvesting efficiency which was higher than the other candidates. The chitinase activity of the freeze-dried powder was kept up about 82.0% of initial activity for the same thermal stress. Subsequently, an optimal formulation recipe was established to maximize long-term storage stability and efficacy. SFB-205 oil-based formulation was stable up to 18 months at room temperature. It showed 96.1% efficacy against 2nd instars of A. gossypii nymphs at 1 day after the treatment in the glasshouse. This novel approach can be a promising method to develop competitive biopesticies in the entomopathogenic fungi, even though it needs to be intensively studied.

The purpose 01' pl'esent study was to examine the molecular events in apoptosis by CoCl2, mimicking hypoxic cond ition and recovering effects by LED ir l'adiation on Human SH-SY5Y neuroblastoma cells The SOUl'ce 0 1' light for ir l'adiation was a continuous-wave LED emitting at a wavelenl양h of 590 nm, and manufactured that ene rgy density was 5 mW!cm2 on sample surface, After ir l'adiation, cell viabi lity was measured with BrdU , cell morphol ogy was examined with Diff- Quik staining, cell signaling was monitored with various apoptosis-related molecules using RNase Pl'otection Assay(RPA) , W11en treated with CoC12, apoptotic induction was found in the SH-SY5Y cells in a concentration-dependent and time-dependent manner , Diff-Quik s taining was revealed that DNA fragmentation re presented apoptosis was examined in CoC12-tl'eated group, Moreover, RPA assay of SH-SY5Y cclls lIs ing val'iolls apoptosis-related molecllles showed that the apoptotic cell population was mcreased J-loweve. there was sorne signifïcant change in LED irradiatied cells aftel' treatement of CoC12 The main mechanism for Lhese a poptosis appearecl to be mito c hondriεt - m ecliated pathway, such as cytochrome- c‘ caspase-9, caspase-3, pro-apototic protein ßax, anti-apototic protein Bcl-2, and death receptor• mediated pathway, such as Fas, cas pase- 8, a ncl TNFRl These results demonstrate that CoCI2 induce apoptosis in SH-SY5Y via different dual apop tosis pathway through death receptor pathway as well as mitochondria- dependent pathway and LED irradiation can recl llces the CoCl2-induced apoptosis by blocking their internal signaling pathway

본 논 문 에서는 직사각형 형태의 사용후 핵연료 저장구조물 에 대한 내진해석을 다루었다. Eulerian 과 Lagrangian 의 두가지 해석방법 을 사용하여， 그 결 과를 비교하였다. Eulerian 접 근 방법에서는 유체 운동에 대한 Laplace 방정식의 경계치 문제를 푼 반면， Lagrangian 접 근 방법에서는 저 장구조물은 고체 유한요소로 모 형화 하였고， 내부유체 는 유체 유한요소로 보형 화 하였다. 유체영역 을 모형화 하는데 사용된 유체요소의 강성올 적 절히 산정하기 위하여 (1 x 1) 의 갑차적 분을 적 용하였다. 응답스펙 트럼 해석 법으로 유체 구조물 상관관계의 내 진 해석 올 수행한 결 과， 뚜 접 근 방볍으로 구한 벽면에 작용하는 유동압이 장 일치 함을 알 수 있었다. 또한 벽면 유연성의 영향을 포함할 경 우， 지 진 발생시 벽면에 작용 하는 유통 압이 크 게 증가할 수 있 음올 알았다.

In this study, a load duration curve was applied to the Jangseongcheon, one of the tributaries of the Yeongsan River, to assess whether the target water quality was achieved. In addition, pollution of the water body was investigated to develop and suggest the optimal management time with respect to polluted flow sections and monthly conditions. The average flow rates of sections JS1 and JS2 were 0.25 m3/s and 1.08 m3/s , respectively. The BOD and T-P for water-quality standards at JS1 were rated at II, whereas the COD and TOC were rated at III, thus indicating a fair level of water quality. By contrast, the BOD at JS2 was rated at III, the T-P at IV, and the TOC at V, indicating poor water quality in this section. The load duration curve was plotted using the actual flow data measured in eight-day intervals for eight years from 2011 to 2018 at locations JS1 and JS2 in the Jangsungcheon Basin. In an assessment using the load duration curve on whether the target water quality was met at location JS1, all of the water quality parameters (BOD, COD, TOC, T-N, T-P, and SS) satisfied the target water quality. By contrast, at location JS2, parameters COD, TOC, T-N, and T-P exceeded target values by more than 50%, indicating the target water quality was not met. The discharge loads of locations JS1 and JS2 were analyzed to identify the reasons the target water quality was exceeded. Results revealed that the land system contributed considerably. Furthermore, the discharge load of JS2 accounted for more than 80% of the load on the entire basin, excluding that of JS1. Therefore, the best method for restraining the inflow of pollutants into the stream near location JS2 must be applied to manage the water quality of the Jangsungcheon.

하천수사용 관리를 적정하게 관리하기 위해서는 객관적인 하천수사용량 자료가 수집되어야 한다. 그러나, 하천수 사용자의 취수여건을 고려하여 유량계 외에 수문조작 등의 간접적인 계측방법을 인정하고 있고, 사용자의 자발적인 보고에 의존함에 따라 자료의 객관화가 어려운 실정이다. 이에 한강홍수통제소에서는 농업용수 사용 비중이 커 하천유량 파악이 어려운 만경강의 고산~봉동 수위관측소 구간에 위치한 어우보 취수로에 V-ADCP를 이용한 계측시설을 설치 및 운영하고 있다. 본 연구에서는 V-ADCP로 측정된 유속을 이용하여 실시간 하천수사용량 산정을 위한 유속분포법의 적 용성을 평가하였다. 이를 위해 Chiu의 2차원 유속분포식의 매개변수 민감도를 분석하고, 실측유량 자료에 기초한 최적 매개변수를 산정하였다. 또한 수위-유량관계법, 지표유속법과 비교 평가하여 유속분포법의 특성을 분석하였다.

본 연구에서는 확률밀도함수의 서식처 적합도 지수를 사용하여 도심하천구간과 자연하천구간에서 유량점증방법론(Instream flow Incremental Methodology, IFIM)을 토대로 피라미 서식처의 생태유량을 모의하였다. 이와 같은 방법을 적용하기 위하여 본 연구에서는 PHABSIM 모형을 사용하였다. 본 연구에서는 서식처 적합도 지수(Kang, 2010)를 기초로 확률밀도함수의 매개변수를 조정하여 확률밀도함수의 서식처 적합도 지수를 개발하여 생태유량을 분석하였다. 그 결과, 도심하천구간에서는 정규분포가 자연하천구간에서는 2변수 log-pearson 분포가 Kang (2010)의 생태유량에 가장 근접하는 경향을 보였다. 확률밀도함수에 의한 서식처 적합도 지수와 하천구간별로 생태유량을 모의하여 확률론적 방법을 적용한 생태유량 산정기법을 제안하였다.

Acute myocardial infarction (AMI) as an acute form and a catastrophic manifestation of coronary artery disease (CAD) is the major cause of morbidity and mortality worldwide. The prevalence and incidence of myocardial infarction can be influenced by demographic, biological and psychosocial factors, and it is rare in childhood and adolescent years. Contrary to the elderly, where cardiac attack is mainly caused by CAD, exercise-induced cardiac attack is relatively more prevalent in a young adult. We report here on a case of ST-segment elevation myocardial infarction (STEMI) in a young adult during vigorous physical activity and we review the relevant literatures.

Background : Platycodon grandiflorum root (PGR) was one of the primary herbs used in a phlegm-relieving herb from the past. Platycoside compounds on PGR may exhibit neuroprotective, antimicrobial, anti-inflammatory, anti-cancer, anti-allergy, improved insulin resistance, and cholesterol-lowering properties. In order to developing a concentrate product that improved the functionality and preference of PGR, it was fermented using lactic acid bacteria (Lactobacillus plantarum N76-10 and 56-12). Methods and Results : The concentrate products were created by PGR-concentrate (PGRC, 60 ºBrix) mixed with fermented PGR-extract (FPGRE, 2 ºBrix) at the level of 0, 50, 100, 150 and 200%. Sweetness and preference were supplemented by other added materials including honey, oligosaccharide, concentrate of jujube (60 ºBrix) and pear (60 ºBrix), and cactus Chounnyouncho extract (2 ºBrix). The products were put into investigation for their preference of taste, antimicrobial activity in accordance with amount of FPGRE. When it comes to preference of taste, the most favor is adding 100% of FPGRE on PGRC. The product added 150% FPGRE exhibited a strong microbial anti-proliferation in all four kinds (Corynrbacterium diphtheriae, Klebsiella pnneumoniae, Staphylococcus aureus, and Streptococcus pyogenes) of bacteria inducing bronchus diseases and was higher antimicrobial activity than concentrate without FPGRE. In terms of the sensory evaluation (taste, texture and visco-elasticity), concentrate mixed with FPGRE (10), jujube concentrate (2), pear concentrate (10), cactus Chounnyouncho extract (10), oligosaccharide (2), honey (1) and xanthan gum (0.02) showed the highest scores. Conclusion : Thus, A PGR concentrate was made by adding FPGRE (100%) and it was increased organoleptic quality, antimicrobial activity. These studies may provide new product development for effective utilization on Platycodon grandiflorum root.

Background : For the GAP standard cultivation, weed occurrence pattern was investigated by planting density and mulching method of Bupleurum falcatum L. Methods and Results : Vinyl and rice straw were used for mulching. The planting density were 10, 20, and 30 cm, and the spacing between plants were 5 ㎝. The amount of weed emergence were examined twice at the end of July and at the end of September. The degree of importance was based on the Braun-Branquet’s dominance value distinction criterion. As a result, the kind of total weeds emerged during the cultivation of Bupleurum falcatum L. were identified as 20 species in 10 families. Amount of weed’s occurrence were the highest in rice straw covering after 10 × 5 ㎝ sowing, and it were lowest in vinyl mulching after 30 × 5 ㎝ sowing. The frequency of weed emergence was highest with 10 species of 8 families in rice straw mulching treatment. On the other hand, the treatment with the least emergence frequency of weeds was 30 × 5 ㎝ sowing after vinyl mulching, and weeds of 7 species of 6 families appeared. In the second survey in September, weeds of 15 species in 9 families were identified in the test field. The amount of weed’s occurrence were the highest in rice straw mulching after 10 × 5 ㎝ sowing, and it were the least in the case of vinyl mulching after 30 × 5 ㎝ sowing. The frequency of emergence of weeds was highest in rice straw mulching after 20 × 5 ㎝ sowing, and the kind of weeds were 11 species of 8 families. The treatment of the lowest emergence frequency of weeds was 30 × 5 ㎝ sowing after vinyl mulching. Three kinds of weeds occurred. Conclusion : From the above results, it is expected that we can suppress the weed occurrence at the planting density of 30 × 5 ㎝ after vinyl mulching at the sowing of Bupleurum falcatum L.

Stent thrombosis (ST) is the sudden occlusion of a stented coronary artery because of thrombus formation. ST is a rare, but can result in life-threatening complications. Early stent thrombosis occurs frequently after primary percutaneous coronary intervention (PCI) for acute ST-segment elevation myocardial infarction (STEMI). Here, we report a case of a 59-year-old man with acute stent thrombosis immediately after primary PCI with drug-eluting stent for acute STEMI.