This study is to identify the maintenance service quality of eco-friendly cars, which are rapidly increasing recently. Research is conducted by synthesizing research from the perspectives of internal employees and external customers by using the service profit chain model. Specifically, it is to study the overall structural relationship between internal customer satisfaction, physical quality, interaction quality, outcome quality, external customer satisfaction and long-term orientation. For the study, 202 questionnaires were collected from internal employees and 204 questionnaires from external customers. The results of testing the research hypotheses targeting the internal employee group are as follows. As a result of testing hypothesis 1, internal customer satisfaction has a significant positive (+) effect on physical quality and interaction quality. As a result of testing hypothesis 2, the service quality of eco-friendly car maintenance has a significant positive (+) effect on each other. As a result of testing hypothesis 3, physical quality and outcome quality have a significant positive (+) effect on external customer satisfaction. The results of testing the research hypotheses targeting an external customer group are as follows. As a result of testing hypothesis 2, in the relationship between eco-friendly car maintenance service quality, physical quality has a significant positive (+) effect on interaction quality, and interaction quality has a significant positive (+) effect on outcome quality. As a result of testing hypothesis 3, interaction quality and outcome quality have a significant positive (+) effect on external customer satisfaction. As a result of testing Hypothesis 4, external customer satisfaction has a significant positive (+) effect only on intention to reuse. Finally, as a result of examining the difference in perception between the internal employee group and the external customer group in eco-friendly car maintenance service quality and external customer satisfaction, it was verified that there was a significant difference only in outcome quality and external customer satisfaction.

Sperm cryopreservation is an important method of assisted reproductive techniques and storing genetic resources. It plays a vital role in genetic improvement, livestock industrial preservation of endangered species, and clinical practice. Consequently, the cryopreservation technique is well organized through various studies, especially on Korean native cattle (Hanwoo). However, the cryopreservation technique of Korean native brindled cattle, which is one of the native cattle species in Korea, is not well organized. Therefore, it is necessary to develop a Supplementary Table technique for the cryopreservation of Korean native brindled cattle. For this purpose, it is important to first evaluate the quality of the currently produced cryopreserved sperm of Korean native brindled cattle. In this study, we randomly selected 72 individual Korean native brindled cattle semen samples collected from 8 different region research centers and used them to evaluate sperm functions. We focused on the quality evaluation of cryopreserved Korean native brindled cattle semen following the measurement of motion kinematics, capacitation status, intracellular ATP level, sperm motility, and cell viability. Then, the values of each of the eight groups were derived from various sperm parameters of nine individual samples, including sperm motility, kinematics, cellular motility, and intracellular ATP levels, which were used to compare and evaluate sperm function. Overall, differences in various sperm parameters were observed between most of the research centers. Particularly, the deviations of motility and motion kinematics were high according to the sample. Therefore, we suggest that it is necessary to develop a standard method for the cryopreservation of Korean native brindled cattle semen. We also suggest the need for sperm quality evaluation of the cryopreserved semen of Korean native brindled cattle before using artificial insemination to attain a high fertility rate.

In recent years, consumers have become more interested in convenient lifestyles, leading to increased use of salted cabbages for preparation of kimchi. This study aimed to investigate the safety of heavy metals, pesticide residues, and foodborne pathogens in commercial salted cabbages in Seoul from August to November, 2014. The survey, which was conducted to determine whether or not salted cabbages were prepared under the highest sanitary conditions, showed that Seoulites are interested in purchasing hygienic and safe salted cabbages. The average amounts (range) of Pb and Cd found in 30 salted cabbage samples were 0.007 (0.000~0.063) mg/kg and 0.004 (0.000~0.012) mg/kg, respectively. The cabbages were analyzed for residues from 285 types of pesticides using the multiresidue method. Residues for pesticides were not detected. Major foodborne pathogens, specifically Escherichia coli, Salmonella spp., Listeria monocytogenes, Campylobacter jejuni, Staphylococcus aureus, Vibrio parahaemolyticus, Enterohemorrhagic Escherichia coli, Bacillus cereus, Clostridium perfringens and Norovirus, were also not detected.

This study was conducted to elucidate the occurrence area and the density of mosquito larvae in Eunpyeong Newtown, Seoul, Korea. Sampling of mosquito larvae was carried out 2 times using a dipping method at 130 points in august, 2014. A total of 1977.6 individuals were collected. Aedini larvae such as Stegomyia albopicta (Skuse) (=Aedes albopictus) or Hulecoeteomyia koreica (Edwards) (=Oclerotatus koreicus) were the most frequently collected than Culex spp. and the Anopheles spp. Mosquito larvae were surveyed in the street inlet (a rain drip box). The positive correlation of the larval density was found with the street inlet point with leaf mold and the depth of water. In the result of spatial statistics analysis, all points larvae collected were clustered and the tendency of spatial autocorrelation was apparent, but points over 20 individuals were dispersed. This result can be assumed that the mosquito larvae are able to occur to stagnant water in all places of this area. To control to mosquito occurrence, we think integrated methods are needed such as biological, ecological, chemical, physical control methods in various aspects.

Mimosa pudica (미모사), Argemone mexicana (멕시코 가시양귀비) Leucus aspara (꿀풀과 일종), Polygonum hydropiper (여뀌), Blumea lacera (국화과 일종) 등 5종의 식물 헥산추출물들의 팥바구미 성충에 대한 살충, 성충우화억제력 및 녹두 종실피해 방제력이 검증되었다. 그 결과, 밭바구미 성충 살충력은 35-69%이었으며, 녹두에 추출물을 처리한 후 성충우화 방제율은 33-63%, 종실 피해 방제율은 13-49%이었다. 추출물의 농도가 증가할수록 살충력, 성충우화 및 종실피해 방제율 등이 증가하는 것으로 나타났다. 이와 같이 5종의 식물 헥산추출물들은 밭바구미 친환경 방제제로 이용가능성이 있을 것으로 판단된다.

Despite that porcine spermatogonial stem cells (pSSCs) have been regarded as a practical tool for preserving eternally genetic backgrounds derived from pigs with high performance in the economic traits or phenotypes of specific human diseases, there were no reports about precise definition of niche conditions promoting proliferation and maintenance of pSSCs. Accordingly, we tried to determine niche conditions supporting proliferation and maintenance of undifferentiated pSSCs for short-term. For these, undifferentiated pSSCs were progressively cultured in different composition of culture medium, seeding density of pSSCs, type of feeder cells and concentration of growth factors, and then total number of and alkaline phosphatase (AP) activity of pSSCs were investigated at post-6 day culture. As the results, the culture of 4x105 pSSCs on mitotically in activated 2x105 STO cells in the mouse embryonic stem cell culture medium (mESCCM) supplemented with 30 ng/ml glial cell line-derived neurotrophic factor (GDNF) was identified as the best niche condition supporting effectively the short-term maintenance of undifferentiated pSSCs. Moreover, the optimized short-term culture system will be a basis for developing long-term culture system of pSSCs in the following researches.

The true bug genus Paromius from Korea is revised taxonomically. It comprises the following three species: P. exiguus (Distant, 1883), P. jejunus (Distant, 1883) and P. gracilis (Rambur, 1839). Among them, P. jejunus (Distant, 1883) is recorded for the first time in Korea. The pest bug injurious to rice culture, known as P. exiguus (Distant,1883), has been recorded erroneously by the previous authors from Korea due to the misidentification, which is rectified here with the support of morphological evidence. All the species of this genus are classified with proper redescriptions, and an identification key to species is provided with illustrations.

Thermal death kinetics was examined to develop a heat treatment method to control the Pine wood nematode, Bursaphelenchus xylophilus, in wood packing materials for export goods. To determine the feasibility of microwave irradiation as an alternative treatment, we performed laboratory experiments on the blocks (200x200x250mm) of Douglas-fir artificially infested with live adults with subsequent 2.45GHz microwave energy irradiation. The 100% mortality of treated Pine wood nematode was achieved above 64℃ of the measured wood temperature, regardless of irradiated time. All measurement points in the blocks reached 64℃ within 84-312 seconds with microwave irradiation by wood weight. Likewise, the 100% mortality of nematodes were observed by hot water treatment on 62℃. We need further experimental verification, but commercial microwave (2.45GHz) treatment is a feasible alternative to conventional heat treatment to control Pine wood nematode on the wood packing materials for export goods.