Expression changes of stress-induced peroxidase (swpa2 and swpa4) and storage root-specific sporamin (spo-A and spo-B) genes were examined using qRT-PCR after treatment with wounding and bacterial pathogens on leaves of sweetpotato (Ipomoea batatas) plants. As a result of examining the expression change in the wounding treatment condition for 48 hours after treatment, which is a representative physical stress, the expression of all genes increased after 12 hours of wounding treatment, but the expression pattern of each gene group showed distinct differences thereafter. Expression levels of swpa2 and swpa4 strongly increased up to 36 or 48 hours after wounding treatment, whereas spo-A and spo-B expression levels strongly decreased after 24 or 36 hours after wounding treatment. Peroxidase and sporamin genes are involved in the early response after wounding treatment and, in particular, the peroxidase swpa2 and swpa4 genes are also involved in the late response after wounding treatment. Gene expression analysis after infection with P. chrysanthemi, which causes softness in sweetpotato, showed that the swpa2 and swpa4 genes were weakly induced after 8 hours and then strongly induced after 20 hours during pathogen infection. Expression of the spo-A gene was weakly induced in the pathogen-treated group after 20 hours, whereas spo-B showed an expression pattern similar to that of the peroxidase genes. The above results indicate that expression of the stress-induced peroxidase gene used in this study is induced not only by abiotic stress but also by biological stress caused by bacterial pathogen invasion and that peroxidase plays an important function in the initial defense response.

Organic scintillator is easy to manufacture a large size and the fluorescence decay time is short. However, it is not suitable for gamma measurement because it is composed of a low atomic number material. Organic scintillation detectors are widely used to check the presence or absence of radiation. The fluorescence of organic scintillators is produced by transitions between the energy levels of single molecules. In this study, an organic scintillator development study was conducted for use in gamma measurement, alternative materials for secondary solute used in basic organic scintillators were investigated, and the availability of alternative materials, detection characteristics, and neutron/gamma identification tests were performed. In other words, a secondary solute showing an improved energy transfer rate than the existing material was reported, and the performance was evaluated. 7-Diethylamino -4-methylcoumarin (DMC), selected as an alternative material, is a benzopyrone derivative in the form of colorless crystals, has high fluorescence and high quantum yield in the visible region, and has excellent light stability. In addition, it has a large Stokes shift characteristic, and solubility in solvent is good. Through this study, it was analyzed that the absorption wavelength range of DMC coincided with the emission wavelength range of PPO, which is the primary solute. Through this study, it was confirmed that the optimal concentration of DMC was 0.04wt%. As a result of performing gamma and neutron measurement tests using a DMC-based liquid scintillator, it showed good performance (FOM=1.42) compared to a commercial liquid scintillator. Therefore, the possibility of use as a secondary solute was demonstrated. Based on this, if studies on changes in the composition of secondary solute or the use of nanoparticles are conducted, it will be possible to manufacture and utilize a scintillator with improved efficiency compared to the existing scintillator.

The safe, efficient and cost-effective decommissioning and dismantling of radioactive facilities requires the accurate characterization of the radionuclide activities and dose rate environment. And it is critical across many nuclear industries to identify and locate sources of radiation accurately and quickly. One of the more challenging aspects of dealing with radiation is that you cannot see it directly, which can result in potential exposure when working in those environments. Generally, semiconductor detectors have better energy resolution than scintillation detectors, but the maximum achievable count rates are limited by long pulse signals. Whereas some high pure germanium detectors have been developed to operate at high count rates, and these HPGe detectors could obtain gamma-ray spectra at high count rates exceeding 1 Mcps. However, HPGe detectors require cooling devices to reduce the leak currents, which becomes disadvantageous when developing portable radiation detectors. Furthermore, chemicalcompound semiconductor detectors made of cadmium telluride and cadmium zinc telluride are popular, because they have good energy resolution and are available at room temperature. However, CdTe and CZT detectors develop irradiation-induced defects under intense gamma-ray fields. In this Review, we start with the fundamentals of gamma rays detection and review the recent developments in scintillators gamma-ray detectors. The key factors affecting the detector performance are summarized. We also give an outlook on the field, with emphasis on the challenges to be overcome.

Spent nuclear fuel still emits radionuclides and high heat that are dangerous to humans. In order to permanently isolate such spent nuclear fuel from human living areas, research is underway to construct a deep disposal system (500 m underground bedrock) consisting of natural and engineering barriers. In this study, plugs, which are engineering barriers consisting of disposal containers, buffer, backfill and plugs were investigated. The plug is one of the engineered barriers made of concrete to block the outflow of radioactive materials and the ingress of organisms, through the tunnel crosssection seals that are eventually discarded. General concrete leachate has a pH of 12.5 or higher and is highly alkaline, which induces dissolution of SiO2 components contained in the buffer and backfill. Dissolved SiO2 causes precipitation and cementation on the surface of the buffer and backfill, reducing performance. Therefore, the use of low-ph concrete is essential for deep, high-level waste disposal sites. Currently, Finland, Sweden, France, Switzerland, etc. have proposed low-ph concrete mix design and performance standards. For example, in Finland, cement, silica fume and fly ash are used as binders and the compressive strength is 50 MPa or more, and the leachate pH is 11 or less. In this research, test specimen fabrication and physical property tests (strength, pH) were performed based on mix design, proposed in Finland, Sweden, France and Switzerland. A cubic (50 mm×50 mm×50 mm) and a cylinder (Ø100 mm×200 mm) specimens were fabricated. Cubic and cylinder were made of mortar and concrete, respectively, depending on whether they included coarse aggregate. General concrete strength shows the characteristic that 70 to 80% of the 28th day of the second order appears on the 14th day of the second order and converges after the 28th day. As a result of mortar strength property evaluation, it increased by 30% from 90th to 28th. pH characterization was evaluated according to the powder dissolution method (ESL method) and leaching method (Leachate, EPA 1315) on cubic (mortar) and cylindrical (concrete) specimens, respectively. Mortar ph was measured at 9.78, a decrease of up to 20% from 90 days to 7 days. The physical property evaluation of concrete is currently underway and shows a trend of increasing strength and decreasing pH according to age. Consequently, we aim to present a low-ph concrete mix design for domestic highlevel radioactive waste disposal sites.