AUDIT-K(한국형 알코올 중독 간이선별 검사법)을 이용하여 일상적으로 생활하고 있는 상당수의 잠재적인 위험 음주자들을 선별 할 수 있었으며 연령, 종교, 결혼여부, 직업, 소득음주사망 가족력, 음주시작 연령, 흡연, BMI(체질량지수)는 통계적으로 유의한 차이가 없었으며, 학력, 음주기간만 통계적으로 유의하였다. 이러한 결과는 교육을 더 받은 사람의 남성이 알코올 소비가 더 높다고 한 선행연구와 부합하는 결과를 나타내었으며 교육수준 또한 무엇을 어떻게 배웠는가에 따른 차이를 살펴 볼 필요가 있음을 시사하며, 교육에 따른 알코올 소비도 각각 다른 결과를 나타냄을 알 수 있다. 또한 나이가 적을수록 술을 많이 마시면 음주기간이 길어질 것이고 기존의 음주시작 연령에 대한 연구와 일치하는 결과가 나타났다. 본 연구에서는 음주 기간이 알코올 사용 장애의 가장 큰 요인이었으며 음주 기간을 단축하기 위한 방법으로는 음주 시작연령을 최대한 늦출 수 있는 국가 정부 제도의 대책 및 교육이 필요하다고 생각된다.

In this study, soy-powder yogurt (SPY) with enhanced levels of γ-aminobutyric acid (GABA) and isoflavone aglycone was produced from sprouting high-protein soybeans (HPSs). The fermented steam-HPS sprouts (0 to 4 cm) were fermented (72 h) with Lactobacillus brevis, and the total free amino acids (FAAs) of the formed mixtures were determined to be 79.53, 489.93, 877.55, 780.53, and 979.97 mg/100 mL in the fermented HPS (FHPS), and the fermented steam-HPS with 0 cm (FSHPS-0), 1 cm (FSHPS-1), 2 cm (FSHPS-2), and 4 cm sprouting lengths (FSHPS-4), respectively. The levels of glutamic acid (GA) and GABA were observed to be the highest, 100.31 and 101.60 mg/100 mL, respectively, in the unfermented HPS (UFSHPS-1, 1 cm) and FSHPS-1 sprouts, respectively. Moreover, the total contents of the isoflavone glycoside form decreased proportionally to the increasing total levels of isoflavone aglycones after fermentation in FSHPS-0, FSHPS-1, FSHPS-2, and FSHPS-4. The levels of isoflavone aglycones were detected as 350.34, 289.15, 361.61, 445.05, and 491.25 μg/g in FHPS, FSHPS-0, FSHPS-1, FSHPS-2, and FSHPS-4, respectively. While FSHPS-1 exhibited the highest DPPH (63.28%) and ABTS (73.28%) radical scavenging activities, FSHPS-4 contained the highest isoflavone aglycone ratio (81.63%). All in all, the FSHPS-1 mixture prepared in this study exhibited high GABA content and functional prosperity, thereby making it suitable for potential applications in the soy-dairy industry.

This study was conducted to investigate the change of workers physiology and sensitivity according to the plant decoration activity using electroencephalographic (EEG), electrocardiogram, and semantic differential (SD) methods on 31 male and female workers in their twenties. Plant decoration activity and writing documents on a computer (WDOC) were carried out for 10 minutes each. The relative slow alpha power spectrum (RSA) has been highly exhibited at most measurement points at plant decoration activity compared to WDOC, and ratio of alpha to high beta spectrum (RAHB) was higher in the parietal lobe responsible for visual function in charge of visual function and showed stable and relaxed effect with little stress or tension. In addition, the relatively fast alpha power spectrum (RFA), which is responsible for visual function, after WDOC was higher than after plant decoration activity in the left parietal lobe (P <.05). Although not statistically significant, the relative high beta power spectrum (RHB) and the ratio of mid beta to theta (RMT), which are stress concentration indexes with tension, were highly regarded as a whole when performing WDOC. There was little difference in heart rate between after both activities. However, high frequency (HF) component indicating parasympathetic nervous system activity was more highly active after WDOC than Planting decoration activity, and the low frequency (LF) component indicating sympathetic nervous system activity tended to be lower after WDOC than after planting activity meaning that plant activity have a sense of stability than WDOC and these results were similar to brain wave analysis. In addition, the sense of comfort, naturalness, calm were significantly higher when the plant decoration activity was performed than when the WDOC was performed in the SD emotional vocabulary survey. These results could be interpreted that plant decoration activity induced more physiologically and psychologically stability and relaxation than WDOC, and enhanced attention ability in appropriate awakening state.

This study aimed to analyze difference in clinical findings, including coronary artery complications, in patients with Kawasaki disease and respiratory symptoms with several respiratory infections. We studied 182 pediatric patients diagnosed with Kawasaki disease. Examinations for respiratory viral polymerase chain reaction were conducted in the group of patients with respiratory symptoms. Echocardiography was perfomed by a pediatric cardiologist, and laboratory findings were evaluated. Clinical manifestations and laboratory findings based on medical records were compared. There were no differences between patients with and without respiratory viral infections with respect to age, male-female ratio, coronary artery complications, Kawasaki disease-specific clinical manifestations, duration of fever, duration of hospitalization, or recurrence rate. There was a significant difference in C-reactive protein levels (55.6 vs. 73.9 mg/L) between the two groups, but the other laboratory findings. The rate of respiratory infections in pediatric patients with Kawasaki disease was similar to those reported in previous studies, and clinical manifestations and laboratory findings were not significantly different between the groups.

This study was taken place to find out the psychological change with comparing before and after the experience of installation of indoor garden with 55 of subjects out of 62 subjects. More than 50% of subject answered that the garden management would be difficult before they experience the installation but the pressure was decreased while the interest of plants and landscape increased, however, the burden of management decreased and the interest in the kinds of plants and landscape design tended to increase after the experience of the installation. The psychological effect of indoor garden of workers was significantly increased with the question of “Indoor garden improves effect of air cleaning.” after the experience of installation and the loyalty was statistically increased with the question of “Intend to invest more fund to purchase indoor plant” (p <.05). As a result of the comparison of two psychological indicators, the recovery index of the state according to experience of indoor gardening was significantly higher after experiencing the indoor garden than before the indoor gardening experience. As the result of sensibility evaluation of pleasant, natural and calm with semantic differential method, workers feel more of them with the indoor garden. In addition, the loyalty was statistically significantly higher in the question “I would be willing to invest more in plant purchase” after experiencing the composition indoor garden (p <.05). This result is similar to the result that the resident’s recovery index, pleasantness, calm feeling, and emotional index are higher the indoor garden in public resting area with indoor garden than without indoor garden. With this study, the perception of indoor garden changed as a result of the worker’s experience of indoor gardening in the workplace and the view of the indoor garden created by the workers. As the psychological healing effect of indoor garden increased, increase in the cost investment for the emotional health promotion of the workers is expected to have a positive effect on the increase of the indoor garden.

Background : This study was conducted to investigate the antioxidant activities and anticancer effect in 80% ethanol extract from foxtail millet (Setaria italica) and proso millet (Panicum miliaceum) and sorghum (Sorghum bicolor) varieties. Methods and Results : These extracts were evaluated for contents of total polyphenol and antioxidant activities using DPPH and ABTS radical scavenging activities. In concentration of 1000 ㎍/㎖, total polyphenol contents of foxtail millet, proso millet and sorghum were 7.68 - 12.74 ㎍․GAE/㎖, 13.01 - 14.79 ㎍․GAE/㎖ and 48.24 - 62.33 ㎍․GAE/㎖, respectively. Sorghum extracts showed strong radical scavenging activity comparable to well-known antioxidant, trolox. Anticancer effect was evaluated for MTT assay using AGS human gastric cancer cells. All extracts decreased growth of AGS cancer cells. Foxtail millet extracts were decreased about 40% of cell viability in all concentrations. Sorghum extracts were decreased cell viability, concentration dependent manner. Especially, Donganme extract (one of the sorghum variety) was the highest anti cancer effect under AGS cancer cells. Conclusion : Based on these results, millet and sorghum has potential health promoting bioactive compounds as functional food.

Background : Ginseng is becoming depleted of virgin cultivation area due to the problem of replant failure. Ginseng farmers have become more burdensome in operating expense because they are more likely to go out to other cities in search of virgin cultivation area. In addition, the quality and yield of ginseng cultivated in one place for many years depend on the rapidly changing climate every year. The purpose of this study was to develop a method for continuous production of ginseng in a facility by solving the problems of replant failure and investigating basic soil composition and growth characteristics of ginseng for 2 - 6 years. Methods and Results : This study was carried out in a 90 ㎝ wide, 50cm deep and 22 m long bed made of sandwich panels in a 90% shaded facility for ginseng cultivation. In the lower part of the bed, a 100 ㎜ pipe for drainage and steam sterilization was installed, and the pearlite was filled at a height of 100 ㎜ as a drainage material. The soil for ginseng cultivation was put into the bed. Soil composition was tested in five combinations including virgin soil, yokto, peat moss, pearlite, and vermiculite with different composition ratios including control. The native seedlings were transplanted and grown from 2 years to 6 years. In the growth characteristics and yield of ginseng, the best treatments were virgin soil 55%, yokto 10%, Peatmoss 25%, Perlite 5% and 5% vermiculite. Also, the bulk density was reduced by 30% compared to the control. Soil pH and EC tended to increase slightly during all treatments. In the ginsenoside analysis, there were no unusual results for the soil composition and they were almost similar. Conclusion : As a method to continuously grow ginseng in the facility, we tried to grow ginseng by filling the soil in the bed. Soil composition should be within the range of chemistry and physics suitable for cultivation of ginseng, and it is necessary to analyze the economy and reduce the operating expense. In the future, researches on soil disinfection and nutrient management methods for continuous use should be continued.

Background : This study was carried out to investigate the changes of the growth of ginseng in the house when the light intensity was increased. Three shading screens were used: shading materials 85%, 77%, and 55% net. Changes in the growth of ginseng and the quality of red ginseng were investigated in these shading materials. Methods and Results : The test site was a three house constructed in the Punggi Ginseng Research Institute, Gyeongsangbuk - do Agricultural Research and Extension Services. The entrance to the house is located east-west, and the structure is double steel frame. The inside of the house is covered with vinyl, and 85%, 75%, 55% shading net is installed for each shading treatment. From the end of June to the middle of August, a 55% shading net was double covered to reduce the high temperature damage. The test material was grafted on March 20, 2013. The management of the irrigation water was from 30 kPa to 10 kPa. The aM-21A data log of Wise Sensing Co., Ltd. was used to measure the cultivation environment in the house. Photosynthesis was measured using Lci Compact Photosyntheisi System, adc, UK. Surveys were carried out in accordance with the inspection standards of the Rural Development Administration. The data were analyzed statistically using SPSS. Conclusion : The summer temperature of shading method was more than 2℃ higher than other treatments in 55% shading treatment and more than 6℃ in autumn. The light intensity of 90 μmol/s/㎡ was lower than that of August, and the light intensity was higher than 400 μmol at the end of September. The 55% shading method showed twice as much light in summer and more than four times in autumn. The amount of photosynthesis increased due to the light intensity of 55% treatment, especially in the morning of May. In the light of shading method, 85% light shading method showed the best growth at 2 years, but 55% at 3 years and more, and 5 years at 5 years.

Background : The area of ginseng cultivation has nearly exhausted in the main ginseng growing area in Korea but virgin fields need for ginseng cultivation due to continuous cropping injury. To count for the decrease of the virgin fields of ginseng cultivation, technical improvement to increase, or maintain the amount of harvest are necessary in ginseng cultivation. The Ginseng Rain-sheltered Shade House is one of the efficient cultivation techniques as reducing the damages caused by natural disaster such as blight, high temperature & humidity, dry, and heavy rain & snow. However a soil description needs to be developed to reduce the period of re-cultivation because ginseng has to be cultivated at the only one ginseng rain-sheltered house without soil or cultivation change. Methods and Results : This study was carried out in the ginseng rain-sheltered house where ginseng had cultivated and harvested one time. Each section of Cultivation of sudan grass+flooding, rice straw+flooding, and flooding was covered with vinyl and then, all areas were solarized through the summer season. The soil temperature was inspected at 5cm, 10cm, 15cm, and 20cm below ground. As a result, the soil temperature of the place with vinyl covered and solarized was approached to 40℃ regularly during the period of high temperature in July and August. After the solarization, the inspection result of Cylindrocarpon destructans spore density checked by Real time PCR was that C. destructans spores were not found at the place where sudan glass+flooding and rice straw+flooding were used. However, the detected number of C. destructans spores in the inspection condition was roughly 9 at the soil samples from the fields which were flooded and solarized, and 33 at those from the repeatedly cultivated fields. The evaluations of aerial part growth by measurements were good in the following order: Sudan grass+flooding, rice straw+flooding, and flooding. Conclusion : The purpose of this study was to reduce the period of ginseng re-cultivation in a ginseng rain-sheltered shade house. The incidence rate of the pathogens distantly decreased by the treatments of sudan glass + flooding, rice straw+flooding, and solarization.

Background : This experiment was carried out to improve the labor-intensive task to construct of the traditional ginseng shading system in Korea. In this experiment the selected shading materials was new 6 kinds that HDPE - knitted- monofilament and taped PE Net, with taking into account of the amount of light and the amount of leakage to screen the UV stabilized 0.3% - 0.6%. Compared with the traditional shading material, the black-shading material (wt. 200g/㎡) and the yellow shading material (wt. 300g/㎡) was suitable for the ginseng growing environments in Korea. Methods and Results : The installation of shading system was the 45 degree slope -2 meter height from the ground with the selected shading materials for the wide structure ginseng cultivation. Temperature and light intensity was measured with a week interval from the surface to a height of 30cm. The two kinds of the 1st selected materials and the traditional black + blue two layer shading materials, aluminum screen, Canada Gintec shading material, total 5 kinds of materials were installed in wide structure. The growing environmental analysis was measured using data logs (watchdog 1600series). Conclusion : In the first selection of shading materials for Ginseng wide structure, the levels of light intensity were higher in order of blue-shading> Aluminum- shading> black-shading order. The temperatures of the under wide structures were lower in order of the black-shading< aluminum-shading screen< blue-shading order, especially the temperature of the black shading material was lower than the outside temperature. For the second selection of shading material of the ginseng wide structure, the black and yellow shading material were superior to the other materials, in the amount of light shading material and growing conditions and the degree of leakage etc. The temperatures of mid-May in wide structures of ginseng cultivation were higher in order of aluminum-screen> Canada shading materials (Gintec Co.) 2 layer material> Yellow shading> black shading order, and also the amounts of light intensity also was shown to be with the same manner

Background : The production of healthy ginseng seedling is a critical part of growing 5-6-year-old fresh ginseng. Recently, for the problem of replant failure, it becomes more and more difficult to find first planted field for ginseng seedling cultivation. In this situation, This study was conducted to examine the possibility of seedling production practices of ginseng by utilizing the multi-beds cultivation system as a way to produce ginseng seedling stably. Methods and Results : This experiment was conducted by installing a 3-layed cultivation beds using 50㎜ sandwich panels. As the 1st and 2nd beds get insufficient amount of light, fluorescent lights were installed in 2 rows to further lighten up. And the 3rd bed used natural light. The bed soil used was formulated by mixing saprolite, yakto and peat moss. The control was cultivated under natural light on 1-layed bed with commercial bed soil for ginseng seedling. The commercial bed soil had pH 7.24, higher than the standard of permission, 6.5; and its calcium and sodium contents were 10.7, and 0.85 cmol+/㎏, respectively, higher than the standard. The available phosphate concentration was 34.0㎎/㎏, lower than the appropriate level of 100 ㎎/㎏. The mixing bed soil also had pH 7.69, higher than the standard but its nitrate and phosphate were within the appropriate range. The intra-facility light quantities of the 1st and 2nd beds were constant at around 100 μmol during days. But the light quantity of the 3rd bed was 51.4 μmol in average daily value. The roots of the 1st-3rd beds were found to weigh 0.58 – 0.73 g while those of the control group, about 0.47g to show heavier root weight of the multi-beds cultivation ginseng than that of the control. As for the yield, the control showed 145 ㎏/10a whereas the 1st-3rd beds showed higher levels between 183 – 228 ㎏/10a. Conclusion : In the multi-beds cultivation of ginseng seedling, fluorescent lamps seem to have provided sufficient light as an artificial light source. The soil for ginseng seedling production is different from ginseng cultivation field soil, additional study will be also necessary to set up the physiochemical range of bed soil for ginseng seedling.

Background : Recently, there is a urgent demand for development of new varieties with enhanced resistance to various biotic/abiotic stresses. In order to develop ginseng varieties with such traits, systematic breeding program and comprehensive field studies are prerequisite. Methods and Results : ‘Cheonmyeong' seeds were collected in 2008 from the farmer field of Buyeo. Physiological investigation and propagation were conducted from 2009 to 2011. It was given the name 'Eumseong No. 8 through the observed yield trial from 2012 to 2013 and local adaptability was carried out from 2014 to 2015. All phenotypes including agronomic characteristics, seed yield, and physiological response to biotic/abiotic stresses were investigated according to the ginseng GAP and UPOV guidelines. Yellow-red leaf and pink berry at maturing stage were observed. The time of emergence, flowering and berry maturity of the ‘Cheonmyeong’ were faster than those of ‘Chunpoong’. Stem length of ‘Cheonmyeong’ was shorter than that of ‘Chunpoong’, whereas stem diameter was thicker than that of ‘Chunpoong’. Main root length was shorter but main root diameter is thicker than that of ‘Chunpoong’. Number of seeds of ‘Cheonmyeong’ was fewer than that of ‘Chunpoong’ but 1,000-seeds weight and stratification rate were higher than those of ‘Chunpoong’. The yield performance of this variety was 661 kg/10 a in local adaptability test for two years, which is 22% higher than that of ‘Chunpoong’. ‘Cheonmyeong’ showed strong resistance to phytophthora blight, mulberry mealybug and nematode and moderate resistance to alternaria blight. ‘Cheonmyeong’ did almost not occur yellow spot of aerial part and rusty skin of root, show moderate resistance at high temperatures. Conclusion : Our study demonstrated that ‘Cheonmyeong’ is an ideal variety with heavier root weight and enhanced stress resistance and contribute will enhance biotic/abiotic stress resistance and increase the farmers' income.

Background : This study was carried out to understand the effect of seedling weight (SW) on growth and flowering in Panax ginseng. Methods and Results : The testing materials were Chunpoong (CP), Yunpoong (YP) and Jakyeongjong (JK). The increase of seedling (1yr) weight led to an increase in ratio of flowering plant and in number of flower per plant. The seed setting rate of two year-old plant (CP, YP, JK) increased with increase of SW at the planting time (PT) and number of flower per plant of three year-old plant (CP, YP) increased also. In the two year-old plant (JK), the ratio of three leaves per plant was 8.8, 19.6, 31.0, 42.0, 44.7 and 58.2%, respectively, in the SW of >0.6, 0.6~0.8, 0.8~1.0, 1.02~1.2, 1.2~1.4 and 1.4g<. The growth of ginseng plant was good with increase of SW at the PT. Conclusion : There was a highly positive correlation between seedling weight and flowering characteristics.

Background : Ginseng farmers prefer first planted field because of replant failure. But the accumulation of nutrients is higher in the first planted field due to using large amounts of chemical fertilizers and manure. The higher phosphate concentration in preparation field decreased emergence ratio and occurred leaf chlorosis in ginseng. The purpose of this study was to develop a technology for reducing phosphate concentration in preparation field of ginseng. Methods and Results : This study was performed in farm's fild located Yeongju in 2015. Treatment of plots consisted of sudangrass, maize, deep plowing + sudangrass and microbes(Rhodobacter sphaeroides, Bacillus subtilis, streptomyces griseus) + sudangrass. sudangrass and maize were sown in early May to 5kg / 10a. Microbes diluted 500 times were treated to drench the soil before sowing crops(400L/10a). The main survey was yield of crop, soil chemical properties, reduction ratio of phosphate and changes in available phosphate. Reduction ratio of phosphate in the plot of maize and Rhodobacter sphaeroides were 27.9% and 24.3%, respectively, which were higher 2∼5% than the control plot. Fresh weight yield was the highest with 11.1ton / 10a in the plot of maize and ratio of dry matter was the highest with 27% in the plot of maize. Conclusion : The phosphate removal ability of maize was better than sudangrass in phosphate accumulation soil. Subsoil reversed using excavators used by some farmers could not mitigate of phosphate and salts concentration in preparation field. The soil that phosphate concentration is more than 1,000mg/kg is required a long-term management.

Background : The Ginseng has been one of the crops that represents Korea, and the main export items in the 1960s, Now it occupies the first exports of agricultural products. The Eco-friendly cultivation of Ginseng was difficult for various reasons. But the cultivation in Green House can be suppressed disease occurrence, when utilizing an environmentally friendly materials has the advantage that can dramatically increase the income by possible organic production. For other crops, it made up a lot of studies on water management, But the studies on water management of ginseng is somewhat lacking. Therefore, this study was conducted to establish appropriate soil- moisture-management-systems within the house of ginseng cultivation. Methods and Results : The type of house is a double steel structure. The first layer was PE film, the second layer was the blue shading net and 75% black shading net. The Soil texture was silt loam and the Soil Series was YEONGOK Series. Install the house on the adjacent paddy soil and upland soil, and planting on April 30, 2011 which was cultivated by the straw mulching. The irrigation start point was the 40, 30, 20kpa, and the end point was treated with 15, 10kpa. Soil moisture measurements were determined by tension meter(Jet Fill) depth 20cm in soil. Growth and Yield Survey was conducted in accordance with the Rural Development Administration (RDA) standard research. Statistical analysis of the results were analyzed using the Microsoft's Excel 2003. Conclusion : In a survey due to differences in soil traits and Irrigation point, from 2 years to 4years of root weight and yield of Ginseng were heavier in the upland, especially 30kpa irrigation point treatment was heaviest than the other treatments. From 5year to 6 Years of ginseng has showed heavier in paddy soil, the most treatment was 30kpa- 15kpa irrigation point. This trend was the same in upland soil. Soil moisture management in the case of silt loam, the humid-state-control was more advantageous than dry-state control in upland and paddy soil.

Background : Correct identification of Panax species is important to ensure food quality, safety, authenticity and health for consumers. This paper describes a high resolution melting (HRM) analysis based method using internal transcribed spacer (ITS) and 5.8S ribosomal DNA barcoding regions as target (Bar-HRM) to obtain barcoding information for the major Panax species and to identify the origin of ginseng plant. Methods and Results : A PCR-based approach, Bar-HRM was developed to discriminate among Panax species. In this study, the ITS1, ITS2, and 5.8S rDNA genes were targeted for testing, since these have been identified as suitable genes for use in the identification of Panax species. The HRM analysis generated cluster patterns that were specific and sensitive enough to detect small sequence differences among the tested Panax species. Conclusion : The results of this study show that the HRM curve analysis of the ITS regions and 5.8S rDNA sequences is a simple, quick, and reproducible method. It can simultaneously identify three Panax species and screen for variants. Thus, ITS1HRM and 5.8SHRM primer sets can be used to distinguish among Panax species.

The high quality of gene set is necessary to study the functional research of genes. Although perilla is cultivated as an oil crop and as a vegetable crop in Asian countries such as Korea, Japan, northeast China and Nepal, the reference genome is absent. To assembly perilla gene set, we sequenced the various tissues of perilla (Perilla citriodora) RNA-seq with Illumina HiSeq platform, generating 548,549,314 short reads. When de novo transcriptome assembly was performed with five samples, 86,396 and 38,413 transcripts were assembled as total and representative transcripts, respectively. Using 1,917,424 proteins at Phytozome ver. 9.1, we annotated the perilla assembled transcripts, and 66,139(76.55%) and 24,030(62.55%) transcripts showed the similarity with known plant proteins (E-value < 1e-10) as total and representative transcripts, respectively. Among the diverse molecular functions, we were interested in the regulatory components, such as transcription factor and transcription regulator. Using this data, we identified 499 transcripts annotated the putative transcription factor differentially expressed transcripts. 165 putative transcription factors were significantly expressed in perilla flower and 121 putative transcription factors in both leaf and flower. This study provides the perilla reference gene set and the understanding of the molecular regulation of transcription factor dependent on the tissue.

In here, we screened drought tolerant varieties with modified leaf water loss rate assay and visual drought tolerant phenotype in the greenhouse conditions with more than 800 varieties. Among these varieties, Samgang, Gumei4 and Apo showed the lowest of leaf water loss rate and strong drought tolerant phenotype. To identify drought QTLs with Samgang variety, we developed the doubled-haploid (DH) population consist of 101 lines derived from a cross the drought tolerant cultivar Samgang and the drought sensitive cultivar Nagdong. To score the drought phenotype degrees of this population, we withheld water for 6 weeks and treated the watering for 7 days. After watering, visual phenotype was observed 1 to 9 degree according to the standard evaluation system for rice, IRRI. Drought sensitive parent Nagdong was almost died and was scored as 9 degree, while tolerant parent Samgang showed slightly leaf tip drying phenotype and was scored as 3 degree in our experimental conditions. Three main QTLs were detected on chromosome 2, 6, and 11 with this visual phenotype. We also measured relative water contend of these population under drought stress conditions, and got one main QTL on chromosome 11. The QTL loci on chromosome 11 with flanking markers RM26755-RM287 has a function for visual phenotype and relative water content under drought conditions.

Rice is a staple food crop for more than half of the world population. Severe losses of rice production was caused by various environmental conditions such as cold, heat and flooding annually. Rice is a highly sensitive to low temperature below 15-20 ℃ because of originating from tropical or subtropical climates. Especially, seedling of rice is easily damaged to low temperature and result in seedling yellowing, growth retardation, reduced tillering and yield losses at last. We used a recombinant inbreeding lines (RIL) population of 384 individuals derived from a cross between Hanareum 2, a highly cold sensitive variety and Unkwang, a cold tolerant variety for molecular mapping of QTLs related to cold tolerance. Seedling discoloration of each lines and parents caused by cold response were investigated in field condition after transplanting. And leaf samples of RIL population were collected for evaluation of chlorophyll content using 80% acetone extraction. The seedling of each lines and parents was subjected to low temperate by 5~13 ℃ during 14 days. The cold recovery score (CRS) of RILs was recorded after 4 days recovery period according to standard evaluation system (SES, IRRI). Total of eight QTLs were detected on chromosome 1, 7, 8, 10, 11 and 12 using cold tolerance traits, chlorophyll content, seedling discoloration and cold recovery score in 384 RILs. The qCRS12, which detected on chromosome 12 between two flanking markers id12002113, id12002563 (1.1 Mbp) showed 25 LOD score with 26% of phenotypic variation of cold recovery score in RILs population. The positive allele contributing to cold tolerance came from the cold tolerant parent Unkwang. The result may provide useful information for a marker-assisted breeding program to improve cold tolerant in rice.