In in vitro fermentation studies, feed samples can either be included in the in vitro rumen medium using filter bags or can be directly dispersed. The objective of this study was to investigate the effects of different pore sizes of filter bags on the rumen fermentation characteristics in an in vitro system. Corn, soybean meal, and timothy were ground to pass through a 1.0-mm screen and were formulated in the ratio of 70:7:23 based on DM, respectively. The formulated experimental diet (2g/DM) was put in F57 filter bags and R510 nylon bags (Ankom®) which pore sizes were 25 and 50 μm, respectively. An in vitro study was conducted to determine the rumen fermentation characteristics for 3, 6, 12, 24, and 48 h and rumen microbial community at 48 h of incubation. A significantly higher production of gas was observed in the R510 bags than in F57 at all the incubation times (p<0.01). IVDMD (p<0.01) and IVNDFD (p<0.01) were significantly higher, whereas pH (p<0.01) and NH3-N (p<0.01) were lower when R510 bags were used. In the VFA composition, acetate and butyrate were significantly higher (p<0.01) in R510 bags, and propionate and total VFA concentration did not differ (p=0.55 and 0.25, respectively) between F57 and R510 bags. The log copy numbers of bacteria and protozoa did not differ (p=0.69 and 0.94, respectively) between F57 and R510 bags, whereas those of fungi were significantly higher in R510 than in F57 bags (p<0.01). Therefore, the use of R510 may reflect actual rumen fermentation characteristics more precisely than those of F57 because increased gas production, nutrient digestibility and acetate, butyrate proportion were founded in R510.

Abstract. Inflammatory myofibroblastic tumor (IMT) is considered as a benign tumor with local aggressive course, consisting of myofibroblastic spindle cells with an inflammatory cells infiltration. IMTs are more usually found in the lung and very rarely in the mandible. We report an IMT of the mandible in a 54-year-old man. The patient complained of pain on the right side of mandible. Radiographically, the lesion was occupied in the right mandible with bone destruction. Although the initial diagnosis was an osteomyelitis, the histopathologic examination and immunohistochemistry revealed it to be an IMT. Histologically, the lesion was composed of inflammatory cells infiltration within a variably fibroblastic or myofibroblastic spindle cell background. Immunohistochemically, spindle cells stained with smooth muscle actin (SMA) and CD68 (KP1), but uniformly negative with desmin and cytokeratin.

Odontoblasts and/or dental pulp cells are responsible for tooth repair as well as dentin formation. Adhesion and migration are critical processes for tissue regeneration. This study was performed to clarify whether Pam3 modulates adhesion and migration of a murine odontoblast-like cell line, MDPC-23 cell and TLR2 signaling is engaged in this process. TLR2 expression in MDPC-23 cells was examined by RT-PCR. Adhesion assay was performed using type Ⅰ collagen-coated plates. Migration ability was determined by a commercial assay kit. Phosphorylation of IκB-α, JNK, p38, and ERK was examined by Western blot analysis. TLR2 was functionally expressed in MDPC-23 cells. Pam3CSK treatment enhanced adhesion and migration of MDPC-23 cells in a dose-dependent manner. Blockade of TLR2 using its antibody restored Pam3CSK-induced adhesion and migration of MDPC-23 cells. These findings indicate that Pam3CSK, an immune activator from gram negative bacteria, can promote adhesion and migration ability of MDPC-23 cells via TLR2.

가축분뇨의 시용은 단순한 비료효과 외에 유기자원의 자원화 및 토양환원으로 자연순환이란 측면에서 다양한 형태의 가축분뇨 이용이 시도되어 오고 있으며 본 시험은 돈분액비 시용 수준이 호밀의 수량 및 사료가치에 미치는 영향과 돈분액비의 적정 시용량을 구명하기 위하여 2000년 10월부터 2002년 5월까지 2년간 축산기술연구소 초지사료과 포장에서 시험을 실시하였으며 그 결과를 하면 다음과 같다. 돈분액비 200% 시용구가 월동 후 생육이 가장 양호하였고 도복

본 연구는 답리작에서 사일리지용 보리 우량품종 선발을 하고자 경기 수원(축산연), 전북익산(호시), 경남 밀양(영시)에서 1999∼2001년까지 3년간 생육단계별로 생육특성과 수량 등을 조사하였다. 수원지역 보리의 출수기는 4월 30일에서 5월 4일까지고 익산지역은 4월 24일에서 5월 3일 그리고 밀양지역은 4월 17일에서 29일까지였다. 그리고 황숙기 수확시 생초수량은 수원, 익산 그리고 밀양에서 각각 24.07∼29.88, 23.18∼32.07 그

본 시험은 가축분 시용 조건에서 교잡종중 우리나라에서 많이 재배하고 있는 장려품종 4종(P 988, Jumbo, TE-Hay-grazer, NC+ 855)을 공시하여 생육시기별, 잎과 줄기의 부위별 질산태 질소 함량과 당도 및 가축 기호도 등을 구명하였다. 가축분은 톱밥과 50:50으로 섞은 발효분으로 ha당 우분 40톤, 돈분 및 계분은 30톤을 파종 보름전 시용하였으며, 파종은 ha당 30kg의 종자를 휴폭 50cm로 1995년 4월 21일에 실시하

This study was conducted to investigate the effects of feed resources and collection system of nonconventional agricultural roughages, and reduction of environment pollution by turning non-conventional agricultural roughages to feed resources and diversif

본 시험은 봄철 속정건조에 의한 건조효과와 건초품질을 구명하여 양질의 건초를 조제코자, 알팔파(Vernal)과 연맥(Swan)을 공시하여 건조방법(건조제, conditioning, 대구조)을 달리하여 1997년 봄 수원 축산기술연구소에서 수행되었다. 수확시기는 6월 3일로 얄팔파는 개화초기, 연액은 춤수기였으며, 화학제인 건조제는 2%를 수확직선 처리 하였고, 물리적인 conditioning은 모델 GMR 2800 trail-type mower cond

Selection of oat variety according to cultivation period is an important to increase productivity of forage crop. To investigate the growth characters and dry matter productivity of oats according to cultivation period, early-maturing oat(EM0) and medium-

This study was conducted to investigate the effects of cutting frequency on dry matter yield and nutritive value between heading and headless varieties of sorghumxsudangrass hybrid in 1998. Treatments were var. TE hay grazer(heading type) and Jumbo(headle