Day-care center is one of living micro-environments for children. In urban area, day-care centers may be influencedby air pollutants emitted from the vehicle exhaust. In this work, diurnal variation of major pollutants and effectof outdoor air on indoor air quality were investigated using real-time instruments for a day-care center locatednear the heavy road. 48-h continuous monitoring at both indoor and outdoor were made in summer. The day-care center equipped with ceiling system air-conditioners was operated from 7:30 a.m. to 19:30 p.m. Indoor CO₂concentration responded greatly to the human activity. Indoor NO₂ concentration shows a big difference betweendaytime and nighttime, implying that outdoor NO₂ may penetrate into the indoor through opening of doors orwindows during the daytime. Indoor to outdoor concentration ratio of submicron particle surface area is <1 dueto the penetration of outdoor ultrafine particles.

본 연구에서는 냉동요구르트 제조 시 trehalose와 sorbitol 첨가가 유산균의 동결변성방지 및 향기성분의 변화에 미치는 영향을 조사하고자 실시하였다. 요구르트를 L. bulgaricus와 S. thermophilus 단독균주 및 혼합균주로 발효시켰을 때 처리구 모두 trehalose와 sorbitol 첨가에 의한 발효과정 중 유산균의 증식효과는 나타나지 않았다. 요구르트를 6주간 냉동저장하는 동안 trehalose와 sorbitol 첨가에 의한 모든 처리구에서 유산균수의 차이가 없었으며, MRS 액상배지에 trehalose와 sorbitol을 각각 2%, 5% 첨가하여 6주간 냉동저장하였을 때는 5% trehalose 처리구의 동결변성방지 효과가 가장 우수한 것으로 나타났다. 냉동저장 기간 동안 고형분 함량이 12%인 처리구와 20%인 처리구의 생균수는 큰 차이가 나타나지 않아 고형분 함량 차이에 의한 trehalose와 sorbitol 효과에는 차이가 없는 결과를 나타내었다. 요구르트의 동결과 해동을 반복하였을 경우 trehalose와 sorbitol 모두 2% 첨가구보다 5% 첨가구가 우수한 동결변성방지 효과를 나타내었다. 전반적으로 Trehalose 첨가구가 sorbitol 첨가구보다 2%, 5% 모두 동결변성방지 효과가 좋은 결과를 나타내었다. Trehalose와 sorbitol을 첨가하여 요구르트를 제조하였을 때 향미성분의 함량은 대조구와 유사하였으며 일반적인 요구르트의 향미를 나타내었다. 냉동저장 중 요구르트 향미성분은 손실되거나, 함량비율의 변화가 발생하지 않았다. 이상의 결과로부터 요구르트의 풍미에는 영향을 미치지 않으면서 냉동저장에 의한 유산균의 동결변성방지제로서의 trehalose첨가가 냉동요구르트의 유산균 안정화에 효과가 있다고 하겠다.

In this study, dietary Rhus verniciflua Stockes were feeded on the porks and Hanwoo beef to determinate sanitation effects. this experiment was performed to measure oxygen contents and total flavor compounds of pork and beef inoculated Pseudomonas aeruginosa in headspace with SPME-GC. The headspace oxygen contents in Hanwoo beef were no difference on the diet supplementation ratio of Rhus verniciflua Stokes during storage at 5℃. Headspace oxygen contents of teratment dieted for 5 and 6months were higher than that of treatment dieted for 3 and 4months. No remarkable difference was observed in headspace oxygen contents in pork between control and treatment during storage at 0℃. Oxygen contents in pork of treatment diet containing 4% Rhus verniciflua Stokes was higher than that of treatment were lower than that of control on Hanwoo beef. Flavor compounds were the lowest in diet supplemented 6% of Rhus verniciflua Stokes during 4 months. Flavor compounds of pork is no change at 0℃. The longer storage periods It had the more increasing total flavor compounds and Rhus verniciflua Stokes supplement ratio had no effects on change of total flavor compounds. Such as this experiment, Flavor compounds and oxygen contents in headspace could be analyzed by SPME-GC method promptly and easily in accordance with, it is expected to apply SPME-GC method to evaluation of food quality.