본 연구에서 흰목이버섯의 추출조건별 추출수율과 기능성 성분의 함량을 비교하여 최적 추출조건을 분석하였으며, 모든 분석에서 R²이 0.9331~0.9462로 유의성을 보이는 것을 확인하였다. 각 독립변수에 따른 추출수율과 ergothioneine, β-glucan 성분을 분석한 결과 추출수율은 추출온도와 추출시료농도에 반비례했으며, 추출시간에는 큰 영향이 없었다. Ergothioneine 성분은 온도가 증가할수록 증가하며, 추출시간은 4.33 h가 가장 높았고 시료량에 큰 영향이 없었다. β-glucan 성분은 온도가 증가할수록 감소했으며, 추출시간에 큰 영향이 없었고 시료량이 21.2 mg/mL에서 가장 높았다. 모든 종속변수의 최대 독립변수는 온도 60 o C, 추출시간 4.33 h, 추출시료농도 16.6 mg/mL에서 추출수율 24.9%, ergothioneine 성분함량 66.8 ug/g, β-glucan 성분함량 34.9 g/100 g으로 나타났다.

This study was designed to evaluate the improvement in exercise endurance by Hovenia dulcis fruit hot water extract (HDWE) for the potential application as a functional food material. The effect was based on the evaluation of the forced-swimming capacity and changes in biochemical parameters in mice. Six-week-old mice were classified into four groups based on a randomized block design: the negative control group, the HDWE 50 mg/kg body weight group, the HDWE 200 mg/kg body weight group, and the 1-octacosanol 50 mg/kg body weight group (n=8 mice per group). The swimming exercise time in the HDWE-50 group was increased at 545±43 sec compared to the control group at 263±28 sec. The HDWE-200 fatigue indicators, lactate acid and ammonia content, decreased to 4.99±0.33 mg/mL and 0.63±0.05 mg/mL, respectively. In addition, the content of glycogen in the liver and muscle tended to increase in the group administered HDWE but was not significant. Likewise, the LDH and glucose contents were increased compared to the control group. The muscle damage indicators ALT (52.7±2.6 U/L) and AST (42.5±5.2 U/L) tended to decrease compared to the control group (76.4±10.2 U/L).

This study was carried out to determine the storage, cryotolerance, heat and drying resistance when the L. acidophilus growing at optimal temperature was changed growth temperature and to apply it to probiotics production so as to help intensify the viability of lactic acid bacteria in the products. L. acidophilus CT 01 was changed temperature at 22℃ and 28℃ after incubated at 37℃ for 18hours. The viable cell number increased to 4.9×108 and 5.0×108 cfu/mL, respectively in the changed temperature groups, but decreased in the control, rapidly down to 7.4×105 cfu/mL in 36 hours. L. acidophilus CT 01 was stored at 4℃ for 12 days after changed temperature at 22℃ and 28℃ for 48hours, changed temperature groups had a survival rate of 112% and 94.43%, higher than that of control (p<0.05). When stored at room temperature, survival rate in the control was 10.88%, very low, but changed temperature groups had very high survival rate, 74.43% and 85.62% (p<0.05). The cryotolerance of L. acidophilus CT 01 appeared higher than changed temperature groups was observed at 37℃ for 66hours. L. acidophilus CT 01 was heat treated at 60℃ for 15 minutes and 30 minutes to examine heat resistance after changed temperature, the heat resistance of L. acidophilus CT 01 appeared higher than control was observed changed temperature groups. L. acidophilus CT 01 was inoculated by 30% to the carrier after changed temperature, and dried at 50℃ for 12 hours. The highest survival rate of L. acidophilus CT 01 was observed changed temperature groups.