소나무의 유전다양성과 유전구조를 추정하기 위해 9개의 ESTP 표지를 13개 소나무 집단에 적용하였다. 소나무 집단의 유전다양성은 관찰된 대립유전자 수(A)가 2.2개, 유효 대립유 전자 수(Ae)가 1.8개, 다형적 유전자좌 비율(P)이 98.8%, 이형접 합도 관찰치(Ho)가 0.391, 이형접합도 기대치(He)가 0.402로 나 타났다. 안강과 강릉 집단을 제외한 11개 집단이 하디-바인베르 그 평형을 만족하였다. 집단간 유전분화도(FST)는 0.057으로, 동위효소나 nSSR 표지분석 결과보다 강하게 나타났다. 군집분 석에서 집단의 유전적 거리와 지리적 분포간에 뚜렷한 연관성 은 확인할 수 없었으며, 집단의 유전분화와 지리적 인접성도 상 관이 없는 것으로 나타났다(Mantel 검증, r = 0.017, P = 0.344). 유전자좌에 대한 FST-outlier 분석을 실시한 결과, 빈도 주의 방법에서는 FST 값이 신뢰하한 이하인 3개 유전자좌와 신 뢰상한 이상인 3개 유전자좌가 특이값으로 추정되었고, 베이즈 방법에서는 3개 유전자좌들만 특이값으로 확인되었다. 두 방법 에서 공히 특이값으로 판정된 3개 유전자좌(sams2+AluⅠ, sams2+RsaⅠ, PtNCS_p14A9+HaeⅢ)중 sams2 표지에서 유래 된 2개 유전자좌는 balancing selection의 영향을 받는 것으로 추정되었다.

In this study, simple sequence repeat (SSR) analyses were utilized for evaluation of genetic diversity and discrimination of 17 accessions. Five cultivars, which were developed from Korea, and 12 foreign accessions, which were collected from China, Japan, Russia and USA, were evaluated by nine markers out of 22 SSR markers. A total of 39 alleles were detected, ranging from 2 to 8, with an average of 4.3 alleles per locus. The expected heterozygosity and PIC values were 0.627 and 0.553, with a range from 0.21 (GB-PG-078) to 0.76 (GB-PG-142) and from 0.19 (GB-PG-078) to 0.70 (GB-PG-142), respectively. Four makers out of nine SSR markers, GB-PG-026, GB-PG-043, GB-PG-142 and GB-PG-177, were selected as key factors for discrimination of Korean ginseng cultivars and foreign accessions. All of Korean ginseng cultivars and foreign accessions were individually by the combination of four SSR markers. Consequently, the SSR markers developed in this study may prove useful for the evaluation of genetic diversity and discrimination of Korean ginseng cultivars and foreign accessions.

The principal objective of this study was to develop a discrimination method using SSR markers in Korean ginseng cultivars. Five cultivars--Chunpoong, Yunpoong, Gopoong, Sunpoong, and Kumpoong--were evaluated by nine markers out of 22 SSR markers. A total of 23 alleles were detected, ranging from 1 to 4, with an average of 2.6 alleles per locus, and an averages of gene diversity (GD) of 0.480. Nine markers were tested in order to distinguish among five Korean ginseng cultivars. Two markers out of nine SSR markers, GB-PG-065 and GB-PG-142, were selected as key markers for discrimination among Korean ginseng cultivars. Two genotypes were detected in GB-PG-065. Chunpoong and Kumpoong shared the same allele type, and Yunpoong, Gopoong, and Sunpoong shared another identical allele type. In the case of GB-PG-142, a specific allele type differentiated from those of other four cultivars was observed only in Sunpoong cultivar. Consequently, the SSR markers developed in this study may prove useful for the identification of Korean ginseng cultivars and the development of ginseng seed management systems, as well as tests to guarantee the purity of ginseng seeds.

This study describes an efficient approach to the development of DNA markers for use in distinguishing the Scrophularia species that have been used as useful medicinal crops. In order to distinguish Scrophularia species, DNA sequences of rpl-5 region in mitochondrial DNA of Scrophularia species were analysed for detecting sequence variations, and the PCR-RFLP method was applied for developing practicable DNA marker patterns. Several DNA variations were detected by the sequence comparison of rpl-5 region among Scrophularia species. Genetic relationship analysis of Scrophularia species was carried out based on these DNA variations. DNA variations of rpl-5 region were revealed that it was significantly efficient in genetic relationship analysis of Scrophularia species. In addition, Scrophularia species tested in this study were completely discriminated by four polymorphic genotypes by PCR-RFLP combined with Tsp509 I (^AATT) restriction enzyme. Our results suggested that DNA sequence variations of rpl-5 region were sufficiently useful for genetic relationship analysis of Scrophularia species. Polymorphic genotypes by PCR-RFLP using the Tsp509 I enzyme will be useful for discrimination of Scrophularia species as a practicable DNA markers.

국내자생 쑥속의 식물을 약용작물 및 산업화에 활용하기 위한 기초자료를 얻고자 본 연구를 수행하였다. 그 결과, 수집된 쑥속 식물들은 사철쑥 (A. capillaris)을 포함한 20종 1아종 2변종 24분류군으로 분류 되었으며, 이를 바탕으로 25개의 화기형질을 이용하여 주성분 분석과 군집분석을 수행하였다. 주성분 분석결과 제1주성분은 전체 분산의 44.73%, 제2주성분은 16.86%, 제 3주성분은 8.88%, 제4주성분은 7.07%의 기여율을 보였으며, 상위 제4주성분까지의 누적 기여율이 77.56%였다. 군집분석 결과, 자방의 퇴화, 아관목, 두화의 크기 등의 주요형질에 의해 크게 3개의 군으로 구분되어졌으며, 화기구조의 식별형질로는 기발표된 Dracunculus, Abrotanum, Absinthium 3절과 완전히 일치하지는 않았으나 국내 자생쑥의 분류형질로 활용이 가능하였다.