The purpose of this study was to analyze the problems that must be resolved in the short and long term to improve rice productivity in Nicaragua, where the current rice self-sufficiency rate is 73%. First, after selecting varieties with high adaptability to various cultivation environmental conditions, it is necessary to thoroughly manage seed purity to supply certified seeds. In rice cultivation technology, it needs to improve seedling standing and weeding effect by improving soil leveling, and watersaving cultivation technology. Also, proper fertilization and planting density must be established in irrigated and rain-fed areas. In addition, it is necessary to strengthen the capacity by collecting and training with the latest agricultural technology information, by revitalizing the union rather than the individual farmer. It is necessary to develop varieties highly adaptable to the Nicaragua cultivation environment, as well as to expand irrigation facilities and cultivation technology suitable for weather conditions June- July in rain-fed areas. Last, it is necessary to maintain the consistency of agricultural policy for continuous and stable rice production, in response to climate change such as drought or intermittent heavy rain.

In order to find out optimum seed production date according to different defoliation and flooding period of Italian ryegrass, this studies with Tetrone were canied out on the experimental field of Honam Crop Experiment Station from 1986 to 1988. Treatment

This study was conducted to find out some conditions for optimum cultivation of Angelica gigas Nakai by theinvestigation of root yield and decursin content from different seeding sizes and accumulative temperature. Accumulativetemperature from April to October was 4,309℃ on altitude 100m , 4,242℃ on 250m, 3,662℃ on 530m, 3,435℃ on 730m,and altitude 530~730m was less 650~870℃ than altitude 100m cultivation areas of A. gigas. Seedling stand rate wasincreased from 86.4% to more than 90% as accumulative temperature decreases, and was increased in above 7㎜ of seed-ling size, and 10% in non-mulching more than PE film mulching. Yield was increased as accumulative temperaturedecreases and in PE film mulching as 310.2㎏/10a. Also, Yield was increased in 7~9㎜ than seedling diameter 5~7㎜ as313.0㎏/10a. Decursin content of primary roots was increased as altitude rises, ie, as 2.55% on altitude 100m, 3.33% on250m, 5.51% on 530m, and 6.24% on 730m. Decursinol angelate content appeared little than decursin content as 1.08%on altitude 100m, 1.37% on 250m, 1.99% on 530m, 2.38% on 730m, and as altitude was heightened, content was increased.

황기의 자가불화합성 타파를 위한 노화수분 처리, NaCl 처리 및 CO2 처리에 대한 결과를 요약하면 다음과 같다. 비닐하우스에서 노화수분은 8월 하순에는 거의 수정이 되지 않았으며, 9월 상순부터 10월 상순까지는 개화 당일 부터 개화 후 2일까지 수정되었고, 9월 하순 개화 당일 수분한 것이 결협율 33.3%, 결실율 86.2%로 가장 양호하였다. 노지에서 노화수분은 대조구는 8월 하순부터 10월 상순까지 결협은 되었으나, 9월 중순과 9월 하순을 제외하고 결실이 되지 않았으며, 9월 하순의 개화 후 1일에 결협율 39%, 결실율 94.9%로 가장 양호하였다. NaCl 처리는 비닐하우스에서는 1% 농도 처리가 결협율은 10월에서 21.3%로 가장 좋았으나, 결실율은 9월 하순 66.7%로 가장 양호 하였고, 노지에서는 NaCl 농도 5%에서 결협율 7.3%, 결실율 90.9%로 가장 양호하였다. 식물생육상을 이용하여 CO2 700 ppm을 처리한 결과 무처리구와 큰 차이 없었다. 결론적으로 황기 자가불화합성 타파를 위하여 9월 중하순에 자화가 아닌 자가화를 개화당일 또는 개화 후 1일에 인공수분해주거나 NaCl 1~5%를 처리해주면 자가불화합성을 타파할 수 있다고 사료된다.

Fifty percent ethanol extract of Lythrum salicaria Linne root (LSR) was tested in vitro on antioxidant activity, and furthermore was investigated on antioxidative and fibrosis protecting activities in CCL4-induced liver fibrosis rat model. Ratio of hepatic GSH/GSSG (reduced glutathione/oxidized glutathione) as bio-parameter of antioxidant level in CCL4 plus LSR-treated rats for 6 weeks significantly increased from 2.8- to 5.7-fold than that of CCL4-treated rats at p 〈 0.05. Thiobarbituric acid reactive substances (TBARS) contents in CCL4 plus LSR-treated rats ranged from 1.57- to 2.19-fold of normal rats and were lower than those in CCL4 plus silymarin-treated rats (1.78~2.46-fold of normal rats) (p 〈 0.05). Amounts of hydroxyproline of liver tissue showing the content of total collagen, a parameter of fibrosis, in CCL4 plus LSR-administrated rat livers were 4.9~8.8μg/mg (-47~-71%, compared with that in CCL4-treated rat livers (16.6μg/mg tissue), which were significantly lower than those in CCL4 plus silymarin-administrated rats being 8.4~11.7μg/mg (-30~-50%). This collagen reducing effect of liver tissue in CCL4 plus LSR-treated rats was supported by histological observation using microscopy assay. From the results, we conclude that the root of L. salicaria have efficient antioxidant potential and effective antifibrotic activities.

광범위한 지역의 재배면적과 생산량을 신속하고 경제적으로 모니터링 할 수 있는 광학(LANDSAT) 중해상도 위성 영상을 활용하여 벼 재배면적, 생육 및 수량을 모니터링 할 수 있는지를 검토하였다. 1. 식생지수와 수량과의 관계를 살펴보면, EVI를 제외한 모든 식생지수와 수량간에는 정의 상관관계를 보였으며, NDVISWIR이나 EVISWIR과는 유의성이 없었다. NDVI가 RVI보다 수량과의 상관도가 다소 높았으며, 수량과 가장 밀접한 식생지수는 NDVIR (r = 0.68)이었다 2. LANDSAT 단일시기 영상(2004년 7월 29일)을 활용하여 서산간척지 지역내 벼 수량을 모니터링한 결과 NDVIR 와 백미수량간에는 1차 직선관계(R2 = 0.46)가 성립하였으며, 필 지중에서 면적이 다소 적거나 주변에 반사특성에 영향하는 요인이 있는 지역을 제외하여 살펴본 결과 추정도가 다소 높아졌다(R2 = 0.66). 3. 논구역 벡터를 사용하여 논구역 정보를 추출하는 기존의 방법 대신 raster 기반의 논구역 masking을 제작하여 논 구역 정보를 추출하였는데 이 방법을 통해 쉽고 빠르게 논구역 정보를 추출할 수 있었다. 4. 연차별 지역적용가능성을 검토하기 위해 1994년 7월 26일 경기도내 7개 시군의 논구역 masking을 제작하여 NDVIred를 추출하고 NDVIR -수량관계식을 이용하여 수량을 추정한 결과 1:1 line에 근접하여 비교적 잘 일치되었다.

An advanced extraction method by ultrasonic extraction with applied solid phase extraction (SPE) has been developed for the determination of simultaneous eight major ginsenosides, namely ginsenosides Rg1, Re, Rf, Rb1, Rg2, Rc, Rb2, and Rd in the root of Panax ginseng. Four extraction methods including n-BuOH reflux extraction (Method A), 70% EtOH reflux extraction (Method B), 50% MeOH reflux extraction with SPE (Method C), and 50% MeOH ultrasonication with SPE clean-up process (Method D) were investigated for the determination of eight major ginsenosides. Total contents of ginsenosides were highest by extraction of Method C as 2.408±0.011%. However, Method D was evaluated as relatively simpler and more efficient method due to short extraction time, small solvent consumption and less expensive, compared to conservative reflux method. Ginsenosides were also satisfactorily separated with good resolution and the accuracy range was between 1.05 and 4.06% as relative standard deviation (RSD) by Method D. SPE condition and HPLC condition were further optimized for determination of eight major ginsenosides by the ultrasonic extraction method. Conclusively, ultrasonic extraction of 2 g sample of ginseng using ultrasonic bath and 1 loading for SPE was evaluated as proper condition for extraction of ginseng.

본 연구는 429점의 식물자원의 항산화활성을 검색하고 새로운 잠재적인 항산화후보물질을 발굴하기 위해 수행하였다. 본 연구에서는 DPPH 라디칼에 대한 소거능과 리놀렌산 산화에 대한 저해활성에 대한 실험이 이루어졌다. 실험결과 Sanguisorba officinalis (flower), Sedum kamtschaticum (flower, root) 그리고 Rumex obtusifolius (flower)가 특히 우수한 활성을 나타내었는데, 이들 식물은 각각 8.12, 9.4, 9.9, 11μg/ml의 DPPH 라디칼에 대한 소거활성 (SC50)과 80.4, 80.1, 84.5, 88.0%의 리놀렌산 산화에 대한 저해활성을 나타내었다. 또한, 1차 선발된 식물 10점에 대한 추가 실험에서, Sedum middendorffianum (root)이 superoxide 라디칼에 대한 소거능 (SC50, 39.4μg/ml)과 CuSO4로 유도된 저밀도지단백질(LDL)의 산화에 대해 우수한 활성 (53.8%)을 나타내었으며, Gleditsia japonica (thorn)는 LDL 산화에 대한 저해활성이 우수 (71.4%)하고 총페놀 함량 (958.5%)이 높은 것으로 확인되었다.

This study was conducted to determine the characteristics of fertilization process and embryo development of Astragalus membranaceus Bunge (Astragali Radix) to provide basic data needed in its breeding. A. membranaceus showed poor seed setting when self-pollination was induced. When artificial pollination was induced, it showed less than 5% bearing in late August, but more than 13% bearing from the beginning of September 4th. The flower size was about 17.0 mm×4.0 mm and pistils and stamens had the same length of 15.0mm at flowering stage. When self-pollination or cross-pollination was induced, pollen tubes extended to an ovule. While pollen tube was extending to the ovule, reproductive cell split and formed two male generative nuclei and a vegetative nucleus. In the case of self-pollination, fertilized embryo was not observed, but was formed in the case of cross-pollination. A. membranaceus is noted to have zygote self-incompatibility. In the case of cross-pollination, fertilization was observed in 6 to 8 h after pollination, where apical cell derivatives split after fertilization. A spherical pro-embryo was then formed three days after fertilization. The seed attained full shape with a seed coat showing its distinctive contour 15 days after fertilization. Thus, A. membranaceus in Leguminosae family is found to have zygote selfincompatibility although its flower shape is shown to match the self-compatibility plant.

국내자생 쑥속의 식물을 약용작물 및 산업화에 활용하기 위한 기초자료를 얻고자 본 연구를 수행하였다. 그 결과, 수집된 쑥속 식물들은 사철쑥 (A. capillaris)을 포함한 20종 1아종 2변종 24분류군으로 분류 되었으며, 이를 바탕으로 25개의 화기형질을 이용하여 주성분 분석과 군집분석을 수행하였다. 주성분 분석결과 제1주성분은 전체 분산의 44.73%, 제2주성분은 16.86%, 제 3주성분은 8.88%, 제4주성분은 7.07%의 기여율을 보였으며, 상위 제4주성분까지의 누적 기여율이 77.56%였다. 군집분석 결과, 자방의 퇴화, 아관목, 두화의 크기 등의 주요형질에 의해 크게 3개의 군으로 구분되어졌으며, 화기구조의 식별형질로는 기발표된 Dracunculus, Abrotanum, Absinthium 3절과 완전히 일치하지는 않았으나 국내 자생쑥의 분류형질로 활용이 가능하였다.