This study was conducted to examine the effects of exogenous gonadotropins (PMSG+hCG) and an antioxidant (cysteine) on in vitro maturation of bovine follicular oocytes. Cumulus-oocyte complexes (COCs) aspirated from 2 to 5 mm ovarian follicles were cultured for 22 to 24 hours in a modified bovine embryo culture medium (mBECM) supplemented with 3 mg/mL bovine serum albumin, to which PMSG (10 IU/mL) + hCG (10 IU/mL) and/or cysteine (0.6 mM) were added. When examined the expansion of cumulus ce1ls at the end of maturation culture, greater (p<0.05) expansion was found after addition of PMSG+hCG (79 to 96%) to mBECM than after no addition (0%), regardless of the presence or absence of cysteine in the medium. The addition of cysteine did not stimulate cumulus expansion, but a high proportion (92%) of expansion was achieved when COCs were cultured after the addition of PMSG+hCG and cysteine to the medium. No difference in the proportion of oocytes underwent germinal vesicle breakdown (initiation of maturation) was found after the addition of PMSG+hCG and/or cysteine to mBECM. However, nuclear maturation (development to the metaphase-II stage) of oocytes was significantly stimulated by the combined addition of PMSG+hCG and cysteine, compared with no addition. In conclusion, both exogenous gonadotropins and an antioxidant are important for nuclear maturation of bovine immature oocytes and these factors have a cell-specific stimulatory action.

These studies were undertaken to evaluate morphological normality and development competence in vitro of hyman oocytes following vitrificatioin using ethylene glycol and electron microscopic grid. Human immature oocytes retrieved from natural and stimulated cycles was vitrified at 0 or 48 h and 0, 8 to 15 or 24 to 28 h after maturation culture, respectively. In oocytes retrieved from unstimulated cycle, no signifciant differences were found in morphological normality (56 to 63%) and fertilization (31 to 37%) rates between the times of vitrification. In stimulated patients, however, more oocytes were morphologically normal when vitrified at 24 to 28 h than when vitrified at 0 or 8 to 15 h after maturation culture. Regardlesss of the hormonal stimulation, high cleavage rates(83 to 100%) were obtained in all treatment groups but did not differ significantly. Twenty to 43% of cleaved oocytes developed to the blastocyst stage at 6 days after IVF. These results suggest that vitrified oocytes from unstimulated and stimulated cycles could develop to the blastocyst stage, regardless of the stages of vitrification.

This experiment was undertaken to investigate the effect of administration of Agyoju or Mokhyang on the maintenance of pregnancy, delivery and sex ratio in the mice in different gestation periods. Adult female mice were administered orally at three different periods, from ovulation to implantation (Exp.1), from post-implantation to organogenesis (Exp.2), and from fetal growth to parturition (Exp.3). In Experiment 1, number of fetus implanted and mean body weight were not significantly different. However, the delivery of male offspring was significantly increased in Agyoju and Mokhyang administrated groups than control group. In Experiment 2, the number of fetuses implanted, live offsprins and their body weight at delivery were significantly increased in the Agyoju administered group than Mokhyang and control groups. In Experiment 3, the number of live offspring and sex ratio were not different in both treatments and control group. However, mean body weight at delivery was significantly increased in both treatment groups than that of control group. These results suggest that 1) Agyoju and Mokhyang have beneficial effects in maintenance of pregnacy, and that 2) The action of unknown component(s) in Agyoju may be related to selection of male spermatozoa for fertilization in vivo, and that 3) the administration of Agyju of Mojhyang during mid-and late-pregnance periods were shown the increment of body weight of live offspring without decrease of litter size.

핵 이식에 공여되는 수핵난자의 효과적인 동결보존을 위하여 한우 성숙난자를 1.0 M dimethylsulfoxide(DMSO) 또는 1.0 M glycerol이 함유된 동결보호제를 이용하여 처리하거나, 동결보호제 처리 후 완만동결법을 이용한 동결융핼르 시행하여 상기 실험처리로 야기되는 투명대 경화현상을 관찰하였다. 도축장 유래의 난소에서 미성숙난자를 채취한 후 10% 소 태아혈청을 함유한 TCM-199을 이용하여 22∼24 시간 동안 체외성숙배양을 이해

Anti-phospholipid antihodies (aPL) have important roles in various pregnancy complications such as recurrent miscarrige, growth retardation, placental abruption and stillbirth. However, their biological actions on preimplantation development of oocytes are still unclear. In this study, we investigated whether either aPL containing sera or phospholipids could affect in vitro fertilization and development of mouse oocytes. Sera used in this study were collected from three patients and the presence of aPL in the sera was confirmed by enzymatic-linked immunosorbent assay. When mouse oocytes were cultured in a serum-free, Chatot, Ziomek and Bavister (CZB) medium (Experiment 1), addition of aPL-containing sera (10%) to CZB medium did not. significantly (P>0.05) influence sperm penetration of oocytes. However, development to the blastocyst stage was significantly (P<0.05) inhibited by serum addition, and formation of morulae (16-23% vs. 58%) and blastocysts (0-4% vs. 21%) was markedly reduced compared with no addition (Experiment 2). In Experiment 3, pronuclear stage embryos were cultured for 96 h in GZB medium supplemented with 1 g /ml phosphatidyl ethanolamine, 1 g/ml phosphatidyl inositol or 1 g /ml phosphatidyl choline. No increase in embryo development was found after addition of the phospholipids to CZB medium. These results suggest that 1) aPL have an inhibitory role in preimplantation development of mouse embryos, and that 2) the action of aPL may be related to a specific phospholid (s) rather than the tested phospholipids in the present study.

본 연구는 2종류의 하우스, 즉 1-2W 형 하우스와 무기둥하우스에, 시설억제재배, 촉성계배, 반측성재배 그리고 여름재배의 4 재배시기에 8개의 오이 품종을 재식하여, 재배시기에 따른 품종들의 암수꽃의 발생, 개체 당 과실수에 따른 퇴화 과실수, 그리고 수량을 조사 비교하여 각 작형에 알맞은 품종들을 추천하기 위해 수행하였다. 시설억제재배에서는 낙합계나 흑진주계 또는 입추백다다기오이가 생육 및 수량이 양호하였고, 촉성재배에서는 겨우살이청장오이, 입추백다다기오이 그리고 가을낙합오이가 추천된다. 반측성재배에서는 청장계 오이나 낙합계 오이가 비교적 양호한 수량을 보였으며 여름재배에서는 남부청장오이나 가을낙합오이가 양호하였다.

본 연구는 여러 오이 품종들의 억제작형에서의 생장과 수량을 조사하여 이 작형에서 적합한 품종을 선발하기 위해 수행하였다. 엽수에 있어서 청장계 오이가 가장 많았고 흑진주계 오이에서 가장 적어 영양생장으로는 청장계 오이가 가장 좋았다. 암꽃수에 있어서 흑진주계 오이가 가장 많았고 청장계 오이가 가장 적었다. 암꽃비율에서도 흑진주계 오이가 가장 높았으며 청장계 오이가 가장 낮았다. 퇴화 암꽃비율에서는 흑진주계 오이가 가장 높았으며 청장계 오이는 0%로 거의 퇴화를 보이지 않았다. 측지제거처리와 측지무제거 처리간 수량은 유이한 차이가 없었다. 수량에 있어서 흑진주계 오이가 가장 높았고 청장계 오이가 가장 낮아 억제작형재배에서 가장 적합한 품종은 흑진주계 오이인 것으로 나타났다.

재배시기, 시설형태 그리고 시설내 계배위치에 따른 가상환경의 차이와 이에 따른 양액계배 오이의 생장 반응을 알아보기 위하여 여름재배와 억제재배, 1-2W형 하우스와 무기둥 하우스에 양액계배를 통해 오이를 패배한 후 시설내 부위별 기온, 상대습도, 일사량과 생장해석을 통해 얻어진 생리적 형질들을 조사하여 비교한 결과는 다음과 같다. 하우스 내부의 평균기온이나 평균상대습도는 하우스 형태간 유의한 차이가 없었으나 일사량은 1-2W형 하우스가 무기둥 하우스에 비해 유의하게 높았다. 일누적일사량은 1-2W형에서는 남서쪽이 무기둥 하우스에서는 북서쪽이 대체로 높았다. 초장과 엽수는 억제재배보다 여름재배에서 높게 나타났으며 엽면적은 억제재배에서 많았다. 상대생장율은 순동화율 및 엽면적비율과 고도로 유의한 정의 상관관계를 보였으며 상대생장율에 대한 기여도는 순동화율이 엽면적비율보다 높은 것으로 나타났다. 작물생장율은 엽면적지수와 고도로 유의한 정의 상관을 보여 엽면적지수의 증대가 단위면적당 생산량의 증대에 크게 영향하는 것으로 나타났다. 전 생육기간의 일평균기온는 상대생장을 및 순동화율과 고도로 유의한 정의 상관관계가 있었다. 억제재배에서는 전 생육기간의 누적일사량과 상대생장을 및 순동화율과 유의한 정의 상관관계가 있었다.

본 연구는 단순배양체계내 소 난포란의 성숙에 요구된는 탄수화물 (glucose, lactate와 pyruvate)을 검토하기 위해 수행하였다. GV(germinal vesicle) 단계의 난구세포로 둘러싸인 소 난자를 단백질, 아미노산 및 호르몬이 첨가되지 않은 modified Tyrodes (mT)에서 24시간, 5% CO2 배양기를 이용하여 성숙배양하였다. Glucose 무첨가군 (0-61%)에 비해 5.6mM의 glucose 첨가군 (71