2022년 1월부터 11월까지 부산지역에서 유통 중인 신선 농산물 210건을 대상으로 리스테리아균 분포 현황 및 병 원성 여부를 조사하였으며, 분리된 균주를 대상으로 혈청 형 및 유전자 지문분석을 통해 역학적 연관성을 확인하였 다. 조사 대상 신선농산물에서 총 40건의 리스테리아균이 검출되었으며, Listeria monocytogenes 등 4종의 리스테리 아균이 검출되었다. 이 중 L. innocua가 22건, L. monocytogenes 10건, L. grayi 6건, L. rocourtiae 2건으로 나타났으며, 이 중 식중독을 유발하는 L. monocytogenes균 은 팽이버섯에서만 검출되었다. L. monocytogenes의 병원 성을 유발하는 유전자인 iap, prfA, inlA, inlC, inlJ 및 hly 6종에 대한 분석 결과, 총 10개 균주 중 6개 균주에서 iap 등 6종의 병원성 유발 유전자가 검출되었으며, 4개 균주 에서 hly를 제외한 5종의 유전자가 검출되어 식중독 발생 잠재위험이 있음이 확인되었다. 지역 유통 식재료에 분포 하는 L. monocytogenes의 유전학적 유사도 및 오염원 추 이를 확인하기 위해 신선농산물에서 분리한 L. monocytogenes 10균주 및 2022년 부산지역 유통 가금류에 서 분리한 L. monocytogenes 2균주를 대상으로 혈청형 분 석 및 PFGE를 실시한 결과, 신선농산물 분리균주의 혈청 형은 1/2a 및 1/2b 두 가지 serotype으로 확인되었으며, 가 금류 분리균주는 모두 1/2a형으로 나타났다. 유전자지문 분석결과, 전체 균주의 유사도는 100-45.7%로 나타났고, 이 중 100% 상동성을 보인 균주들은 동일 생산농장 또는 동일지역 유래 팽이버섯에서 분리되어 오염원의 출처가 같음을 추측할 수 있었다. 신선농산물 분리균주와 유통 가금육 분리균주와의 유사도 확인 결과, 일부 팽이버섯 분 리균주와 가금육 분리균주의 유사도가 90.9-84.6%로 나타 났다. 농산물 및 축산물 생산시설간 오염원 이동 및 교차 가능성을 유추할 수 있었다.

The purpose of this study is to investigate dietary pattern and health status related to food habit and degree of fatigue of evening college students. A sample of 476 evening college students in Chonbuk area were surveyed by questionaire examining their food habit, nutrient intakes, degree of fatigue and cornell Medical Index(CMI). In this study, the male and female students were classified into two groups, respectively, the first group is not having jobs and the second is having jobs. The results were summerized as follows: 1. Evaluation status of food habit score indicated that the subjects were included in Fair and Poor group generally. Food habit score of female was significantly higher than score of male. For female students, food habit score of job group was significantly higher than score of non-job group(p〈0.05). 2. Average intakes of calorie and protein were 1869.1 kcal and 68.3 g in male and 1612.4 kcal and 58.3 g in female. Other nutrients, such as vitamin B1(1.1 mg), vitamin B2(1.2 mg) and ascorbic acid(41.0 mg) in male, iron(13.5 mg), vitamin B2(1.1 mg) and ascorbic acid(46.5 mg) in female were lower than the korean RDA. 3. The means of degree of physical, mental, neurosensory and CMI scores were 3.1, 1.9, 1.5 and 4.7 in male and 4.0, 2.4, 2.4 and 7.1 in female, respectively. For female students, though significancy was found between job group and non-job group in physical, neurosensory fatigue and CMI score(p〈0.05, 0.01), but there were not significant differences in these score for male students. 4. There were significant positive correlation between three types of fatigue and CMI, whereas score of food habit was negative correlation for degree of physical, mental fatigue and CMI. The intakes of protein and vitamin B1 were significantly related to the mental, neurosensory fatigue and CMI.

This study was surveyed the preference for the regional food and food choices according to Region, Age, Sex. The subjects were 943 residing in Seoul and Cheon Nam region. The summarized result are as follows; The survey about the preference for the regional food showed that subjects in Cheon Nam has higher preference for the Cheon Nam food and subjects in Seoul for the Seoul food respectively. The most subjects marked very high preference scores about familiar food. The preference for the regional food and food choices were effected by main growth region. Subjects brought up in Cheon Nam like Cheon Nam food especially fresh raw fishes(WHOI) and fermented fishes(CHOT GAL) more than those brought up in Seoul, although both of them reside in Seoul now. But they were brought up in Cheon Nam, subjects residing in Cheon Nam or Seoul now showed no difference in preference for Cheon Nam food. Subjects in Cheon Nam showed higher preperence for fresh raw fishes(WHOI), fermented fishes(CHOT GAL), especially in older men.