Background : This study was performed to investigate the morphological and growth characteristics of tetraploid Polygonum multiflorum L. with colchicine treatment, and to clarify its effective induction and time for the increase of rhizome hypertrophy and effective components. Methods and Results : The induction of tetraploid P. multiflorum were done with colchicine treatment of 0.1, 0.5, and 1.0% conc. and the time was treated with 12, 24, and 48 hrs, respectively. DMSO 1% is treated for the effective penetration of colchicine with constant-temperature oven at 24C. Leaf explant was added the HR-A solution stained with HR-B solution, and then determined whether DNA contents of the doubles or not. Seed stand rate showed lower tendency as higher treatment concentration and as delayed the treatment time. When treated in DMSO 1% with 48 hr, seed stand was so small rate with 2%, and treated in 0.5% with 12 hr, seed stand was recorded higher rate with 33%. When treated in 0.5% colchicine with 24 hr, chimera and tetraploid induction rates were highest and obtained 29 individual chimera and 5 individual tetraploid. On overground growing, mean stem diameter of tetraploid (11.3 ㎜) is two times thicker than that of diploid (6.4 ㎜), with the vigorous growth. leaf length and width of tetraploid was 9.1 and 6.2 ㎝, respectively, and that of diploid was 6.8, and 5.1 ㎝, respectively. Stem diameter of a scape was also more thicker tendency in tetraploid than in diploid, chlorophyll contents over 29% existed in tetraploid. Investigated result from stoma no. and size of leaf with 400 magnification, stoma no. of diploid (26 individual) was four times higher than that of tetraploid (7 individuals), the size of tetraploid (38 ㎛) is twice longer in size of diploid (18 ㎛) and stoma area are broaden in four times. Seed length of tetraploid (3 ㎜) is longer than that of diploid (22 ㎜); but, seed width of tetraploid (22 ㎜) is widen than that of diploid (14 ㎜) in seed size comparison. That is, seed length is bigger in 1.4 times and seed width is bigger in 1.6 times, and seed surface area is broaden in two times. Conclusion : From above results, overground growing of tetraploid P. multiflorum is fairly better than that of native P. multiflorum. In the future, this tetraploid variety will promote to register when recognize the excellence than native species after tested the disease tolerance, underground growing characteristics, and key index component.

Background : The effect of ridge-up bed cultivation and stem training method on the plant growth and yield of Cynanachum willfordii was investigated. Methods and Results : Domestic variety of Cynanachum willfordii was tested at Chungbuk ARES under the following conditions. Nonsupporting (custom) and I-form supporting was used for stem training method, and ridge height was set as 30 (custom) and 60 cm (high ridge). Planting spacing was adjusted to 30 (interrow spacing) × 20 cm (intrarow spacing), and other major management was followed the method of standard cultivation for medicinal crop. Investigated result from leaf characteristics, leaf length was longer in high ridge cultivation (HRC) as 11.1 cm than custom cultivation (CC) as 10.6 cm. Leaf width is proved to be 12.8 (HRC) > 11.2 cm (CC). Leaf number is proved to be 294 (HRC) > 254/plant (CC), with higher number of 44/plant at HRC and weight/10 leaves were more heavy at HRC (4.9 g) than that of CC (2.6 g). It was more fruit setting at HRC over 15/plant. According to the stem training method and ridge height from nonsupporting cultivation, main root was 4.0 (CC), higher than that of HRC over 0.5/plant. However, root length was more longer in HRC (28.6) than that of CC (25.0 cm). Main root diameter was also showed more thicker pattern in HRC. From staking cultivation, root number of HRC was 7/plant, it was recorded more 3/plant than that of CC, and it was also same pattern in main root length and root diameter. By the standard of commercial root, yield of living roots in nonsupporting cultivation were 59.0g/plant (HRC), it was more heavy over 10.4g/plant than that of CC. In staking cultivation, HRC were recorded as 74.2g/plant, more heavy 6.9g/plant than that of CC. Yield from I-form support stem training and ridge-up bed cultivation of HRC was higher approxmiately 52.7% than that of CC. Conclusion : Overall growth by high ridge cultivation of I-form support was good and yield of HRC was also increased over 52.7% than that of CC.

This experiment was conducted to clarify the effect of double layer mulching on reducing the labor required to weed control and Leaf sheath training of the garlic cultivation. Six mulching methods(non-mulching, transparent P.E., rice hull+transparent P.E., sawdust+transparent P.E., rice straw+transparent P.E., black P.E. film+transparent P.E.) were used for the experiment, and transparent P.E film was removed on April 10. Weed occurrence was in the order of black P.E. film<sawdust

충북농업기술원 마늘연구소에서는 2000년부터 수집한 유전자원 중 한지형인 ‘단양종’ 보다 조숙다수성이며 불완전추대의 특성을 가진 계통을 5년 동안 지속 선발하여 ‘단산’ 품종을 육성하였다. ‘단산’ 마늘의 추대 특성은 불완전 추대형으로 ‘단양종’의 완전 추대형과 뚜렷한 구별성을 갖는다. 따라서 화경이 출현하지 않아 수확 전에 쫑을 뽑을 필요가 없으며 숙기도 6일 정도 빨라 수확기 때 장마를 피할 수 있는 장점을 갖고 있다. 2차 생장비율은 3.3%로 ‘단양종’이 17.3% 보다 매우 낮아 이차생장율이 적은 상품성 마늘을 생산할 수 있다. ‘단산’의 통의 크기는 ‘단양종’ 보다 크고, 쪽 껍질의 색은 갈색을 띠며 안토시아닌 줄무늬가 존재한다. 수량은 1,131 kg/10 a로 ‘단양종’ 보다 18% 정도 높다.