Male crickets (Order Orthoptera), producing sound for courting females and threatening other males, were chosen to perform researches for the better understanding of the microstructures of sound producing organs. It is known that cricket only makes sound for mating-related events. In this research, two patterns out chirping patterns were observed and analyzed. Each chirp was made of several nodes of waves, each node indicates a movement of wings – friction caused by file and plectrum located on wings. Although both wings possess file and plectrum, only the file on right wing and plectrum on left wing are used to produce sound. Each file consists of 126 teeth, where plectrum gets hooked. The teeth located on file have consistent gap between each other, proportional to the wave nodes acquired – except that the gap in the second region among equally divided six regions were shorter. In case of the usage of file, a cricket mostly uses second region to fifth region, since the teeth in first and sixth region are smaller. Since most of the researches made are mainly focused on the correlation between sound production and behavioral pattern, the current research project aims to reveal and provide thorough understanding on the sound producing organs of cricket, to suggest possible biomimetic applications in our daily lives.

Ips acuminatus is a minute bark beetle found in forest and can cause economic damage to pine and spruce trees. This beetle has well developed sensory system respond to both of visual and chemical stimuli. Both sexes have a pair of faceted compound eyes and another pair of knobbed antennae, work together to collect vital information. The antennae look similar in both sexes and consist of scape, pedicel, and segmented flagellum. The pedicel is the first segment by which the antenna is attached to the head and the scape is set in a membranous socket and surrounded by the antennal sclerite on which a single articulation occurs. The beetle’s antennae enlarge abruptly at the last segment of a flagellum giving the antenna a knobbed appearance. There are a number of sensory receptors, including olfactory and mechanical receptors. Here, the fine structural characteristics of the antennal sensory organs in male and female bark beetle Ips acuminatus (Coleoptera: Curculionidae: Scolytinae) were analysed with field emission scanning electron microscopy (FESEM).

Sperm cell development in spider species undergoes in testicular cyst, containing certain number of cells per cyst. As a germ cell matures through entire stages of spermatogenesis, testicular cysts rupture and produced spermatozoa are transferred in a form of cleistosperm. When mature spermatozoa pass through deferent duct, it is known that various types of seminal secretions are released into the lumen to provide auxillary functions to the mature sperms – such as nutrition, protection, or sperm release inside the female body. However, a peculiar type of seminal secretion was observed in this study. In the lumen of deferent duct, encapsulated seminal secretions are observed along with coiled sperm cells. Since the capsule is quite thick – as thick as the one of mature sperm cell, it is thought that the secretion capsule would be transferred as well along with the sperm cells into the spermathecae probably activiating sperm cells through decapsulation. Also, this study revealed that sperm storage in deferent duct occurs in droplet-by-droplet basis; which suggests possible sperm inducing mechanism. Since spider uses pedipalps in copulation to transfer its sperm cells, spider has to fill it accordingly. In other words, stored sperms in deferent duct are released in a droplet at once.

Entomopathogenic fungi formulated as wettable powders and suspension concentrates have been sprayed to crop pests for pest management. However, the use of fungal granules to control paddy field pests has not been fully explored. Herein, several Beauveria bassiana isolates (ERL 1170, 1578 and 836) were produced as granules using a millet-based solid culture. The granules were applied to the rice nursery 3 days before transplanting and their control efficacy against rice water weevils was determined in paddy fields. The solid cultures produced ~1×108conidiag-1ofmilletgrains10daysaftertheinoculation. The granules were applied to the soil in the rice nursery 3 days before the rice seedlings were transplanted in the paddy fields. Rice in plots with granules of ERL1578 had 17.3% leaf damage (74% control efficacy) 14 days post application, whereas rice plants in the non-treated control had 66.5% damage. Rice plants treated in the nursery with ERL1170 and ERL836 had 52~54% damage. In the rice plots previously treated with ERL1578 the smallest numbers of larvae and adults were observed 38 days post application. In laboratory conditions, ERL1578-treated larvae were tuned pink and covered with mycelial mass. Applications of millet-based B. bassiana granules on rice nursery soil can be an effective and efficient biological control strategy for the management of rice water weevils. This method is relatively inexpensive and requires less labor compared to practices involving the spraying of fungi directly on rice in paddy fields.

This study is intended to examine the tDCS and Morris Water maze training in Alzheimer’s disease(AD) rats on Tau protein expression. Experiment groups were divided into four groups and assigned 16 rats to each group. Group Ⅰ was a control group(AD induced by scopolamine); Group Ⅱ was a experimental control group(AD injured by scopolamine and treatment tacrine); Group Ⅲ was a group of tDCS application after AD injured by scopolamine; Group Ⅳ was a group of morris water maze training after AD injured by scopolamine. In cognition test, the outcome of group Ⅱ was significantly lower than the groups(p<.001). and group Ⅲ, Ⅳ were significantly low result at 14 days(p<.05). In histological finding, the experimental groups were destroy of micro vessels and finding of cell atropy and swelling. Group Ⅲ, Ⅳ were decreased in degeneration of liver and kidney cells. In immuno- histochemistric response of BDNF and tau protein in hippocampus, BDNF expression of Group Ⅱ was more increase than the other groups. and increase of BDNF expression was Ⅲ, Ⅳ were higher than group Ⅰ at 21 days. Tau protein expression of Group Ⅱ was more decrease than the other groups. and decrease of Tau protein expression was Ⅲ, Ⅳ were lower than group Ⅰ at 21 days. These result suggest that improved tDCS and morris water maze training after scopolamine induced is associated with dynamically altered expression of BDNF and Tau protein in hippocampus and that is related with cognitive function.

Mealworms, Tenebrio molitor (L.) is used as an important animal feed additive for growth promotion and health management, but potentially exposes to fungal infection. In this work, virulence of two species of entomopathogenic fungi against the insect, and the relationship between abiotic features and virulence were investigated. Secondly our consideration was also given to the effect of chemical fungicides on conidial germination for risk control. Between Beauveria bassiana (Bb) and Metarhizium roberstii (Mr) (previously M. anisopliae), Bb isolates had much higher virulence (~100% mortality in 3~4 days after the treatment), rather than Mr isolates in laboratory assays. Next, fungus-treated mealworms were kept at wheat bran at 20, 25, 30 and 35℃ with 3, 6, 9 times of water spray to the feeds for set-up of different humidity conditions. Inoculation of fungi to mealworms was conducted by fungal spray and feeding methods, which resulted in higher virulence in feeding method. In the feeding method, all temperature treatments except 35℃ showed high virulence against mealworms, but any significant relationship between virulence and humidity was not observed. In the chemical fungicide screening, fluazinam (CAS No. 79622-59-6) and mancozeb (8018-01-7) significantly inhibited the germination of Bb and Mr conidia. This work suggest that contamination of wheat bran with fungal pathogens, particularly B. bassiana may induce mycosis of mealworms, but introduction of effective fungicides possibly reduce fungal infection.

본 연구는 mushroom complete medium(MCM) 액체배지에 수삼 추출물(GE, 65°Bx)을 첨가하여 면역활성이 증진된 노루궁뎅이버섯(Hericium erinaceum) 균사체를 배양하고, 균사체로부터 활성다당성분을 분획하고자 하였다. MCM에 대하여 GE를 5, 10과 15%(v/v) 첨가한 액체배지에서 균사체를 배양하고, 각각의 조다당획분(HE-GE-5-CP, HE-GE-10-CP와 HE-GE-15-CP)으로 분획하여 면역활성을 측정한 결과, HE-GE-10-CP는 HE-GE-5-CP와 HE-GE-15-CP보다 높은 활성을 나타내었으며, GE를 첨가하지 않은 MCM에서 배양된 균사체 조다당획분(HE-CP)보다 유의적으로 증진된 면역활성을 나타내었다. 또한, HE-GE-10-CP의 DEAE-Sepharose CL-6B 분획물 중 가장 높은 활성을 나타낸 HE-GE-10-CP-II획분은 대조군인 HE-CP의 어떠한 획분보다도 유의적으로 높은 면역활성과 암 전이 억제활성을 나타내었다. 한편, 활성획분인 HE-GE-10-CP-II는 arabinose, rhamnose, galactose, glucose와 uronic acid(molar ratio; 0.34:0.26:0.99:1.00:0.39)로 구성되어 있으나, 대조군인 HE-CP의 동일용매 용출획분으로서 HE-GE-10-CP-II보다는 활성이 낮은 HE-CP-II는 fucose, mannose, galactose와 glucose(molar ratio; 0.32:0.55:1.00:0.96)를 함유하여 다른 구성당 분포를 나타내었다. 따라서 노루궁뎅이버섯 균사체 액체배양에서 수삼 추출물 첨가는 균사체의 구성당 변화를 통한 면역활성 증진에 관여하는 것으로 사료되어 기능성 소재 개발에 유용할 것으로 사료된다.

We would like to present three aspects of Jeju global governance of World Heritages in Jeju Island. To accomplish its international designations, including the UNESCO triple crown, intangible cultural heritages, and RAMSAR wetlands sites, the Jeju Government has created a GIS system where 5 types of land-use have been successfully designated and managed for the preservation of the Environment: 1) Absolutely Preserved Zone, 2) Relatively Preserved Zone, 3) City Planning District, 4) Preserved Eco-System District, 5) Preserved Scenery District. Through these 5 land-use categories, we can see how as a whole, the land is managed, protected, and kept pristine.Jeju must embody the three goals of the Jeju Declaration: “Scaling up Conservation,” “Nature-based Solutions,” and “Sustainability in Action.” Jeju has the ability to mobilize communities to work for biodiversity conservation and sustainable development. Jeju also has the ability to halt biodiversity loss and apply nature-based solutions to conserve biodiversity, enhance resilience, and so improve the well-being of the people on the island and in turn to improve the well-being of the people on the planet. To actualize practices of 4 Jeju Motions (M067; Establishment of an Integrated Management System for UNESCO Protected Areas, M 108 : Supporting the Sustainability of Jeju Haenyeos as a Unique Marine Ecology Stewardship, M 162 : The Development of an Evaluation and Certification System for World Environment Hubs and unapproved motion, M 181 : Protection of the People, Nature, Culture and Heritage of Gangjeong Village), Jeju National University opens Peace Island Leadership School. We also need to involve in an initiativeto integrate each component into a framework of a World Environment University grounded in the Island province of Jeju in South Korea. Within that framework the establishment of a Green Growth and Travelism Institute is a priority element. As a torchbearer of the green growth 2050 vision, this is envisaged as the centre of a virtual global network of related organizations and institutions.

The gene encoding an esterase enzyme was cloned from a metagenomic library of cow rumen bacteria. The esterase gene (est2R) was 2,120 bp in length, encoding a protein of 516 amino acid residues with a calculated molecular weight of 57,286 Da. The molecular weight of the enzyme was estimated to be 57,000 Da by SDS-PAGE. Est2R shared 35.6% amino acid identity with esterase (CAH19079) of uncultured prokaryote. The Est2R was most active at 20-40°C, and showed optimum at 30°C and pH 8.0. The most activity of Est2R for the different chain length of p-nitrophenyl ester group as substrate was p-nitrophenyl acetate. Moreover, the enzyme was found to be most active without organic solvent, followed by 98% active with ethanol, and the enzyme activity was highly affected by the acetonitrile. The enzyme was significantly inhibited by Zn2+ but stimulated by Ca2+. So, novel esterase gene est2R is likely to obtain from cow rumen metagenome and supposed to use for industrial purpose.

A carboxymethyl cellulase gene, cel5B, was cloned, sequenced, and expressed in Escherichia coli. pRCS20 in E. coli was identified from metagenomic cosmid library of cow rumen for cellulase activity on a carboxymethyl cellulose agar plates. Cosmid clone (RCS20) was partially digested with Sau3AI, ligated into BamHI site of pBluescript II SK+ vector, and transformed into E. coli DH5α. The insert DNA of 1.3 kb was obtained, designated cel5B, which has the activity of hydrolyzation of CMC. The cel5B gene had an open reading frame (ORF) of 1,059 bp encoding 352 amino acids with a signal peptide of 48 amino acids and the conserved region, VIYEIYNEPL, belongs to the glycosyl hydrolase family 5. The molecular mass of Cel5B protein expressed from E. coli DH5α exhibited to be about 34 kDa by CMC-SDS-PAGE. The optimal pH was 8.0, and the optimal temperature was about 50℃ for its enzymatic activity.

장수명 핵분열생성물인 79Se와 99Tc는 자연수 중에서 용해도가 클 뿐더러 음이온으로 존재하여 방사성폐 기물 처분장에서 주요 관심핵종들로 고려되고 있다. 본 연구에서는 KURT 지하수의 다양한 pH와 산화-환 원 조건에서 셀레늄과 테크네튬의 Solubility Limiting Solid Phase (SLSP)로 알려진 FeSe2와 TcO2의 용해 도를 측정하였다. 또한, 지화학코드를 이용하여 실험과 유사조건에서 이들의 용해도와 주요 화학종을 계 산하였다. 실험 및 계산으로부터 pH 8∼9.5와 Eh=-0.3∼-0.4 V 조건에서 FeSe2의 용해도는 1x10-6 mol/L 이하이며, 주 용해 화학종은 HSe-로 판단된다. TcO2의 경우는 pH 6∼9.5와 Eh<-0.1 V 영역에서 용해도와 주 용해 화학종이 각각 5x10-8∼1x10-9 mol/L와 TcO(OH)2로 나타났지만, Eh=-0.35 V조건에서는 주 용해화학종이 pH가 10.5∼12와 12이상에서 각각 TcO(OH)3 -와 TcO4 -로 계산되었다.

Antimicrobial peptides are widely found in living organisms and are known to play a critical role in innate immunity. Numerous antimicrobial peptides from diverse species appear to be effective against pathogenic microorganisms of bacteria, fungi, protozoa, and viruses. Because antibiotic resistance is a global health issue in the fight against pathogenic microorganisms, there has been an urgent need for development of new antibiotic substances. In the current study, we performed yeast two hybrid screening using Beclin1 bait in order to find new peptide antibiotics from a random peptide library. Two candidate peptides from the screening were expressed in a yeast secretory system of Pichia pastoris and tested for any antimicrobial activity against Staphylococcus aureus, MRSA, MRSA2242, MRSA2250, Lactobacillus casei, and Lactobacillus acidophilus. Disc clear zone assay and spectrophotometric analysis revealed that the two peptides exert a decent activity against the pathogenic bacteria, in contrast to minimal effect on the commensal Lactobacillus strains. Taken together, this study presents novel peptides with antibacterial activity against the pathogenic forms of Staphylococcus aureus and suggests the possibility that these peptides, upon further characterization, may be developed as clinically useful antibiotics.

We present a study searching for globular cluster systems (GCSs) of two face-on low surface bright- ness galaxies (LSBGs), UGC 5981 and UGC 6614. Based on HSTWFPC2 images of F555Wand F814W filters, we detect 12 and 18 GC candidates for UGC 5981 and UGC 6614, respectively. Although these two LSBGs have very different bulge properties, they have very similar specific frequencies (SN) of 0.1. However, SN ~ 0.1 is quite small even for their late morphological types, albeit within errors. This suggests that LSBGs have had star formation histories lacking dominant initial starburst events while accumulating their stellar masses through sporadic star formation activities.

Anthocyanins are naturally occuring phytochemicals and the main components of the coloring of plants, flowers and fruits. They are known to elicit antioxidative, anti-inflammatory and cancer preventive activity. In this study, we investigated anthocyanins in black / yellow soybean seedcoats using different methods of detection - thin layer chromatography (TLC), capillary zone electrophoresis (CZE) and HPLC analysis. The anthocyanins in soybean seedcoats were extracted by five independent methods of extraction and the aglycons (anthocyanidins) of the corresponding anthocyanins were prepared by acid mediated hydrolysis. The anthocyanin / anthocyanidin in black soybean seedcoat showed characteristic TLC mobility, CZE electrophoretic retention and HPLC migration time while little of anthocyanins were detected from yellow soybean seedcoat. The extracted anthocyanins showed pH dependent retention time in CZE and spectral change in UV-Vis spectrum. HPLC analysis of the hydrolyzed extract of black soybean seedcoat identified the presence of four anthocyanidins. The major anthocyanin in black soybean seedcoat was cyanin (cyanidin-3-O-glucoside), with the relative order of anthocyanidin in cyanidin > delphinidin > petunidin > pelargonidin.

원자전달 라디칼 중합을 이용하여 poly(epichlorohydrine) (PECH)를 주사슬로 한 양친성 가지형 공중합체를 합성하였다. PECH로부터 poly(methyl methacrylate)(PMMA) 및 poly(butyl methacrylate)(PBMA)의 가지형 중합이 성공적임을 1H NMR과 FT-IR분석을 통해 확인하였다. 합성한 가지형 공중합체에 KI나 LiI 염을 도입하였을 때, ether 신축진동 피크가 낮은 wavenumber영역으로 이동하였으며, 이는 배위결합 상호작용 때문이다. PECH-g-PBMA 복합체의 이온 전도도는 PECH-g-PMMA 복합체에 비해 항상 높게 나타났는데, 이는 고무상인 PBMA 사슬의 높은 이동성으로부터 기인한 것으로 확인되었다. 최고 이온전도도 값은 질량비 10 wt%의 KI가 도입된 PECH-g-PBMA 전해질체에서 2.7 × 10 -5 S/cm로 나타났다.

Poly(vinyl chloride)-g-poly(styrene sulfonic acid) (PVC-g-PSSA) 가지형 공중합체를 합성한 후, 이를 이용하여 80℃에서 열적으로 환원하여 은 나노입자를 제조하였다. 반응 시간을 바꿈에 따라 다양한 구조의 은 나노입자를 제조하는데 성공하였다. 1시간 정도의 짧은 반응 시간에서는 가지형 공중합체의 미세 상분리 구조를 크게 변화시키지 않고 5 nm 크기의 작은 은 나노입자가 생성되었다. 5시간 정도의 중간 반응 시간에서는 30 내지 50 nm 정도의 크기를 갖는 은 나노입자가 생성되었다. 18시간 정도의 긴 반응 시간에서는, 은입자가 뭉친 허리케인 모양의 은 집합체가 관찰되었다.

본 논문에서는 마이크로스트립 패치 RFID 태그 안테나를 제안하였다. 안테나는 짧은 스트립과 오픈 스터브, 패치, 그라운드로 구성되어있다. 제안된 안테나는 FR-4기찬을 사용하였고, 유전율은 3.5, 탄젠트 손실은 0.02이다. 전체적인 안테나의 크기는 길이가 100mm, 넓이는 26mm, 높이는 6mm이다. 기판의 두께는 5.93mm, 그라운드와 패치의 두께는 0.035mm이다. 제안된 안테나의 중심주파수는 UHF대역인 915MHz에서 형성하고 있다.

국내 벼 건조기간의 기상조건에 적합한 열펌프를 설계, 제작하여 기본 성능을 측정하고, 건조온도 20-50oC 범위에서 벼 건조실험을 통하여 건조특성 및 소요에너지를 분석하였다. 열펌프는 건압축 냉동 사이클에서 냉동효과는 173.8 kJ/kg이었으며, 냉매순환량은 49.6 kg/hr이었다. 따라서, 성능계수는 5.5로 표준냉동사이클에서 냉매 R-22의 성능계수 4.0에 비해 높은 값을 나타내었으며, 목표 건조공기의 온도 30oC 및 상대습도 40%에 도달하는 시간은 6분 및 7분으로 만족할 만한 수준이었다. 건조온도와 곡온과의 온도차이는 건조온도 21.9oC에서는 약 1.5oC, 건조온도 48.7oC에서는 약 8.5oC로서, 건조실에서 상승한 곡온은 템퍼링실에서 충분한 냉각이 이루어지는 것으로 판단되었다. 건조온도 21.9, 30.7 38.8 및 48.7oC에서 건조속도는 0.29, 0.61, 0.85 및 1.25%/hr로 나타나 상용건조기와 유사한 수준이었다. 건조온도 21.9oC에서 소요에너지는 325 kJ/kg, 건조온도 30.7, 38.8 및 48.7oC에서는 667, 692 및 776 kJ/kg로 나타나 외기조건에 따라 건조소요에너지의 차이가 발생했지만, 화석연료를 사용하는 상용 화력건조기의 벼 건조 소요에너지 4,000-5,000 kJ/kg에 비해 평균 86% 절감되는 것으로 나타났다.