For licensees who face the decommissioning project for the first time, even if they can utilize their experience in operation, they should be well prepared and assessed for the risks of dismantling activities reflecting the characteristics of decommissioning. This can be included in the risk management of the decommissioning project, but what we want to discuss in this study is the evaluation of the industrial risk of the actual work before the dismantling work is carried out. We would like to focus more on the review of dismantling activities subject to industrial risk assessment and a series of processes for risk assessment. The dismantling work plan will need to obtain approval from the supervisory department before work on the Systems, Structures, and Components (SSCs) can be carried out. At this time, risk assessment may be included among many safety-related required documents, which are divided into radiological and non-radiological risks. The target activities at Level 1 level can include preparation for dismantling and maintenance of facilities, dismantling big components, removing the contamination of concrete structures, managing radioactive waste, etc. In addition, it can be composed of preparation work, removal of connections, lifting/installation, cutting, radiation/radioactivity measurement, and withdrawal as detailed work stages of each item’s activities. For domestic nuclear decommissioning projects, two major performance organizations, licensees and contractors, must be considered. Regarding risk assessment, the licensee will have a supervisory department controlling decommissioning activities and an HSE department at the site, and a process will need to be established in consideration of the contractor’s work organization. Therefore, activities in the risk assessment process may be established. In this study, risk assessment was reviewed as safety-related matters to be considered when carrying out the dismantling work. Safety-related risk assessment is a necessary procedure for performing practical dismantling activities, and this should be considered well in advance. Therefore, work activities and criteria were established for risk assessment, and the performance process was assumed to apply them. In terms of the performance organization and the responsibilities and roles of the processes to be performed by each organization were constructed, and this can be referred to in the process of preparing for the decommissioning project.

CRISPR/Cas9-induced knock-out/-in can be occurred at specific locus in the genome by non-homologous end joining (NHEJ) or homology directed repair (HDR). Here, we demonstrate the targeted insertion into the specific loci of embryo fertilized by semen from transgenic cattle via CRISPR/Cas9 system. Recently, we published on the efficient generation of transgenic cattle using the DNA transposon system (Yum et al. Sci Rep. 2016 Jun 21;6:27185). In the study, eight transgenic cattle were born following transposon-mediated gene delivery system (Sleeping Beauty and Piggybac transposon system) via microinjection. In the analysis of their genome stability using next-generation sequencing, there was no significant difference in the number of genetic variants between transgenic and non-transgenic cattle. All the transgenic cattle have grown up to date (the oldest age: 33 months old, the youngest age: 15 months old) without any health issue. One of transgenic male cattle expressing GFP reached puberty and semen was collected. Over 200 frozen semen straws were produced and some were used for in vitro fertilization (IVF). On seven days after IVF, expression of GFP was observed at blastocyst stage and was seen in 80% of the embryos. Another application is to edit the GFP locus of the transgenic cattle because long-term and ubiquitous expression of transgene didn’t affect their health. In one cell stage embryos produced using GFP frozen-thawed semen, microinjection of sgRNA for GFP, Cas9, together with donor DNA that included RFP and homology arms to link the double-strand break of sgRNA target site into fertilized eggs resulted in expression of RFP. This indicated that the GFP locus of transgenic cattle shows potential candidates for stable insertion of the functional transgene. Knock-out/-in for editing GFP locus using CRISPR-Cas9 might be a valuable approach for the next generation of transgenic models by microinjection. In conclusion, we demonstrated P-112 that transgenic cattle via transposon system are healthy to date and germ-line competence was confirmed. The GFP locus will be used as the potential target site for future gene engineering via genome-editing technology. Finally, all those animals could be a valuable agricultural and veterinary science resource for studying the effects of gene manipulation on biomedical research and medicine. This work was supported by BK21 PLUS Program for Creative Veterinary Science and Seoul Milk Coop (SNU 550-20160004).

The suspension system of special tracked vehicle is using hydraulic piston pump to adjust track tension and control vehicle position change. During operation of vehicle on rough field, failure of suspension control was occurred due to the piston pump failure. In this study, investigation was performed to analyze the cause of hydraulic piston pump failure. Main reason of piston pump failure is strong peak pressure and insufficient structural safety of shoe. The static stress analysis considering peak pressure was performed to find the weak point of the shoe. From the result, it is confirmed that pass hole of lubrication is the weakest point. Improved piston shoe shows 27% decrease in maximum stress and satisfies the design target which is less than 40% of stress margin.

상부 탑재형 노내계측기(TM-ICI) 개발은 원자로하부헤드 대신 원자로상부헤드로 계측기를 삽입함으로써 중대사고 위험을 줄이기 위해 진행 중이다. 이 개발 과제의 일환으로, NUREG/CR-6909와 Code Case N-761의 두 방법에 따라 TM-ICI 노즐에 대한 환경피로평가가 수행되었다. TM-ICI 노즐은 level A, level B 및 시험 조건에서의 과도조건에 따른 하중을 받는데 이에 대해 피로평가를 해야 한다. 원자로냉각재환경이 고려된 TM-ICI 노즐의 누적사용계수는 1이하로 평가되었고, 이는 ASME Code 허용기준을 만족한다.

본 논문에서는 원통형 쉘에 부착된 노즐의 구조 건전성평가를 수행하고 그 결과를 비교하기 위해 2차원(2D)과 3차원(3D)해석이 수행되었다. 현재 원자력 발전소에서 사용되는 3개의 노즐을 구조 건전성평가를 위해 선정하였고, 각각 노즐은 내부압력, 온도변화 및 외부하중을 받는다. 내부압력에 대한 2D 해석은 1.5이상의 계수 값을 이용하거나 응력집중 계수를 적용하여야 하고, 온도변화에 대한 2D와 3D 해석결과는 피복재의 유무와 상관없이 서로 거의 비슷하며, 외부하중에 대한 WRC Bulletin 297에 의한 해석결과는 3D 해석결과보다 더 보수적임을 확인할 수 있었다.

현재 전 세계적으로 설계단계에서 부식 생성물과 방사성 핵종의 양을 예측하는 프로그램에 대해서는 개발되거나 개발중인 프로그램이 다양하다. 그러나 원자력 발전소 해체 시 발생하는 방사화 부식생성물의 양을 평가하는 코드에 대한 개발은 이 루어지지 않고 있어 정확한 산정에 어려움이 있다. 원자로 용기, 원자로 구성품 및 인접 구조물에서의 특성 원소의 중성자 조 사로 인한 방사화재고량을 평가하기 위해서는 원자로의 고정된 구조물을 대표하는 모든 영역에서의 평균 중성자속과 구조 물의 물질조성 및 원자로 운전이력 등을 이용하여 평가해야 한다. 본 논문에서는 설계단계에서 사용되는 1차 계통의 부식생 성물과 방사성 핵종의 양을 예측하는 CORA, PACTOLE, CRUDSIM, CREAT 및 ACE 코드를 분석하였다. 향후 연구에서는 제 염해체 폐기물 발생량 평가에 대한 사용가능성과 개선점을 찾아 부식생성물량 산정에 정확성을 높이고자 한다.

It is generally accepted that chronic stress impairs female reproduction. It negatively affects ovarian function and the number of ovulated oocytes. Chronic stress lowers the number of retrieved oocytes. Ovarian follicular development is regulated by both pituitary-derived gonadotropins and intraovarian regulatory factors. The main corticosteroids are cortisol, cortisone, 11-deoxycortisol and corticosterone, cortisol being one of the most commonly used welfare and stress physiological indicator. In this study, we investigated the effect of cortisol level on progesterone patterns and ovulation in the dog. Cortisol and progesterone level of serum were analyzed by radioimmunoassay. The day of ovulation was considered as the day when serum progesterone concentration was 6.0∼8.0 ng/ml. In vivo dog oocytes were collected by flushing oviducts of mixed-breed bitches at three days after ovulation. We classified dogs as having group 1 (cortisol level, 0 ≤ or < 2 μg/dl), group 2 (corisol level, 2 ≤ or < 4 μg/dl), group 3 (cortisol level, 4 ≤ or < 6 μg/dl) and group 4 (cortisol level, 6 μg/dL ≤). The patterns of progesterone were not different in four cortisol groups. The average numbers of retrieved oocytes was not different in four cortisol groups. These results suggest that different cortisol levels on estrus dogs do not affect ovulation, number of ovulated oocytes and progesterone changes.

The objective of this study was to monitor health conditions of four genetically identical somatic cells cloned Labrador retriever puppies by estimation of body weight and analysis of hematologic and serologic characteristics. Naturally ovulated oocytes and donor cells were used for somatic cell nuclear transfer (SCNT). Donor cells and enucleated oocytes were followed by electric fusion, chemical activation and surgical embryo transfer into the oviducts of surrogate females. Two recipients became pregnant; two maintained pregnancy to term, and four live puppies were delivered by Caesarean section. The cloned Labrador retrievers were genetically identical to the nuclear donor dog. The body weight of clone-1, -2, -3, and -4 was increased from 0.66, 0.40, 0.39, and 0.37 kg at birth to 6.2, 6.6, 6.2, and 6.0 kg at 8 weeks of age, respectively. Although clone-4 had lower numbers of RBC than reference range, the most of RBC and WBC related heamatologic results of cloned puppies were not different when compared to reference range. In serological analysis, Glucose, ALP and inorganic phosphate level of four cloned puppies was significantly higher than the reference ranges. However, there was no significant difference among four cloned dogs. This study suggests that cloned puppies derived from SCNT did not have remarkable health problems, at least in the growth pattern and hematological and serological parameters.

Phosphoprotein Enriched in Astrocytes (PEA15) is a 15kD-sized intracellular signaling protein, highly expressed in astrocytes and constitutively expressed in peripheral tissues. Recently it was found that PEA15 expression was elevated in patients suffering type 2 diabetes and suggested to be involved in the syndrome of insulin resistance. To investigate the functional role of PEA15 for the control of blood glucose level, we produced a transgenic pig over-expressing mouse PEA15 (mPEA15). As a model animal, pig has many advantages. They have a higher fecundity and a short generation time and are physiologically similar to human. Using the transgenic pig, we carried out a series of experiments to establish a link between PEA15 expression and the insulin resistance. Our results suggested that, compared with control pig, mPEA15 pig has, (1) a higher blood resistin level, (2) a lower cell membrane-embeded GLUT4 level, and (3) a lower glucose clearing ability based on oral glucose tolerance test (OGTT). When our results combined, it can be concluded that mPEA15 over-expressing pig has many symptoms of insulin resistance and these pigs will become a useful disease model to investigate diabetes mellitus in the near future.

본 연구는 실리콘계 정포제의 특성에 따른 폴리우레탄 폼 지수제의 cell 구조와 흡수량 변화를 알아보기 위하여 6종의 정포제를 사 용하여 폴리우레탄 폼 지수제를 제조하여 FE-SEM으로 분석한 결과 실리콘 정포제의 실록산 주사슬 말단에 PO n개가 결합되어 있는 DC-193, DC-2585, DC-5125, DC-198의 cell 구조는 close cell로 확인이 되었고, 실리콘 정포제의 실록산 주사슬 말단에 EO n개가 결합 되어 있는 DC-5043과 DC-5598은 open cell 구조로 나타났다. 또한 cell 구조 변화에 따른 흡수량 에서는 close cell의 크기가 가장 세밀하고 균일한 DC-193의 흡수량이 가장 적게 나타나 내수성이 가장 우수한 것으로 나타났으며 open cell의 크기가 가장 크게 형성된 DC-5043의 흡수량이 가 장 많은 것으로 나타났다. 이들의 방수성능을 콘크리트 구조물을 모사하여 시험한 결과 누수가 없음을 확인하였다.