The primary therapeutic approach for Brucella species infections has mainly been based on antibiotic treatment. However, the development of vaccines for brucellosis control remains controversial. Furthermore, there is currently no licensed vaccine available for human brucellosis. This study aims to evaluate the effect of a combination of recombinant protein vaccines against Brucella (B.) abortus infection using a mouse model. Two B. abortus genes, namely dapB and gpm, were cloned and expressed in competent Escherichia (E.) coli DH5α using the pCold-TF vector. Successfully cloned vectors were subjected to PCR amplification using specific primer pairs. The apparent sizes of dapB and gpm were detected at 807 bp and 621 bp, respectively. Besides, the purified recombinant proteins dapB and gpm were detected using SDS-PAGE electrophoresis with correct sizes of 82.86 kDa and 87.61 kDa, respectively. These recombinant proteins were used to immunize mice as a combined subunit vaccine (CSV) to elicit host immunity against B. abortus infection. Mice immunized with CSV exhibited increased proliferation of CD4+ and/or CD8+ T cells at week 7th and 9th before sacrifice, in comparison to the control group. Notably, CSV immunization showed a significant decrease in bacterial burden in the spleen compared to the control group. Altogether, CSV using dapB and gpm induced host adaptive immune response against Brucella infection, suggesting its potential as an effective new subunit vaccine candidate.

Extensive research and testing continue to be conducted for the development of vaccines targeting zoonotic diseases such as brucellosis. In this study, the potential of the DapB as a recombinant protein vaccine to effectively combat Brucella abortus 544 infection in BALB/c mice was evaluated. Western blotting assay results showed that recombinant protein DapB reacted with Brucella-positive serum, indicating its potential immunoreactivity. In vivo results showed that the peripheral blood CD4+ and CD8+ T cell population significantly increased in the DapB-immunized mice group after the first, second and third blood collection, compared to the control group that received PBS. Additionally, at the fourth blood collection, an increase in CD4+ T cell activation was observed in three vaccination groups compared to PBS negative control group. These results indicate the potential of DapB in stimulating cellular immunity. Fourteen days after infection, the bacterial load in the spleen was evaluated. The reduction in bacterial replication in the spleen by both DapB and RB51 highlights their protective efficacy against Brucella infection. These findings contribute to the ongoing efforts in developing effective vaccines against brucellosis and provide valuable insights for further research in this field.

Foot-and-mouth disease (FMD) is a very deadly and highly contagious disease that occurs in various cloven-hoofed animals. Korea imports vaccines including non-vaccinated serotype for the vaccine bank. The two company vaccines imported from 2019 to 2021 were evaluated through multiple tests based on national standard assay. SN titers for the vaccine of company A were more than 1.5 log10, those of company B vaccine in the geometric mean were at least 1.72. All imported vaccines exceeded the evaluation criteria, and the potency of each vaccine was above its own company standard. Stockpiled vaccines for emergency may help to control non-vaccinated serotypes of FMD outbreak nationally.

In NPP (nuclear power plant), boric acid is used as a neutron absorbent. So radioactive boric acid waste are generated from various waste streams such as discharge or leakage of reactor coolant water, floor drains, drainage of equipment for operation or maintenance, reactor letdown flows and etc. Depending on KHNP, 20,015 drum (200 L drum) of concentrated boric acid waste were stored in KOREA NPP until 2019. In previous study, our group suggested the waste up-cycling process synthesizing B4C neutron absorber using boric acid waste and activated carbon waste to innovatively reduce radioactive wastes. Radioactive activated carbon waste was utilized in off gas treatment system of NPP to capture nuclide such as I-131, C-14 and H-3. Activated carbon waste is treated as low-level radioactive waste and pre-treatment system for removing nuclide from the activated carbon waste is needed to use B4C up-cycling process. In this study, microwave treatment system is suggested to treat the activated carbon waste. Activated carbon waste was exposed to microwave for a few minutes and temperature of the waste was dramatically increased over 400°C. Nuclide in the activated carbon waste were selectively removed from the waste without massive production of secondary off gas waste.

Lysophosphatidic acid (LPA) is a bioactive lipid messenger involved in the pathogenesis of chronic inflammation and various diseases. Recent studies have shown an association between periodontitis and neuroinflammatory diseases such as Alzheimer’s disease, stroke, and multiple sclerosis. However, the mechanistic relationship between periodontitis and neuroinflammatory diseases remains unclear. The current study found that lysophosphatidic acid receptors 1 (LPAR1) and 6 (LPAR6) exhibited increased expression in primary microglia and astrocytes. The primary astrocytes were then treated using medium conditioned to mimic periodontitis through addition of Porphyromonas gingivalis lipopolysaccharides, and an increased nitric oxide (NO) production was observed. Application of conditioned medium from human periodontal ligament stem cells with or without LPAR1 knockdown showed a decrease in the production of NO and expression of inducible nitric oxide synthase and interleukin 1 beta. These findings may contribute to our understanding of the mechanistic link between periodontitis and neuroinflammatory diseases.

The amount of temporarily stored spent nuclear fuel in South Korea will be reaching saturation in a near future. Therefore, it is an urgent issue to construct a spent nuclear fuel storage system. In order to construct the storage system, some coastal environmental characteristics such as temperature, pH, and chemical composition of sea water in South Korea have to be evaluated and predicted because they can affect in deterioration of the storage system. However, in South Korea, the coastal environmental characteristics of area where the storage system is likely to be built are not well established until now. In this study, a time-series deep-learning algorithm is developed using the Long-Short Term Memory (LSTM) algorithm to predict and evaluate the coastal environmental characteristics based on the wellestablished data from Korea Meteorological Administration (KMA) and Ministry of Oceans and Fisheries (MOF). As a result, by developing the predictive model to evaluate the coastal environmental characteristics, we intend to apply it for site evaluation to construct the spent nuclear fuel storage system or many other applications related to the nuclear as well.

Mesenchymal stem cells in the dental pulp exhibit a tendency for differentiation into various dental lineages and hold great potential as a major conduit for regenerative treatment in dentistry. Although they can be readily isolated from teeth, the exact characteristics of these stem cells have not been fully understood so far. When compared to twodimensional (2D) cultures, three-dimensional (3D) cultures have the advantage of enriching the stem cell population. Hence, 3D-organoid culture and 3D-sphere culture were applied to dental pulp cells in the current study. Although the establishment of the organoid culture proved unsuccessful, the 3D-sphere culture readily initiated the stable generation of cell aggregates, which continued to grow and could be passaged to the second round. Interestingly, a significant increase in SOX2 expression was detected in the 3D-spheroid culture compared to the 2D culture. These results indicate the enrichment of the stemness-high population in the 3D-sphere culture. Thus, 3D-sphere culture may act as a link between the conventional and 3D-organoid cultures and aid in understanding the characteristics of dental pulp stem cells.

본 연구는 원통형 종이포트를 활용한 토마토 육묘시, 염스트레스를 활용하여 고온기 도장 억제가능성을 검토하기 위하여 수행되었다. 시험구는 K2SO4, KCl과 KH2PO4을 각 5, 10 dS·m-1로 처리하였고, 또한, 토마토 모종에 고염도의 칼륨을 처리하여 수분 및 저온스트레스 환경에서의 적응성 및 생존성을 조사하였다. 조사결과, 처리 농도가 높아질수록 지상·지하부 건물중, 옆면적, 순동화율 (NAR)이 감소하고, 경경과 충실도는 증가하였다. 수분 스트레스 처리 이후, 대조구는 심한 위조현상을 보였지만, KCl처리구는 양호하였다. 상대수분함량은 대조구에서 23%, KCl처리구에서 8% 감소 하였다. 또한, 대조구에 비하여 KCl 처리구는 저장시(9, 12 및 15°C) 모종의 손상 비율이 낮았다. 이와 같은 결과로 보아, KCl과 같은 고농도의 칼륨 처리가 원통형 종이포트 토마토 육묘의 도장 억제에 효과적이며 환경 스트레스 내성을 향상시키는 것으로 판단된다.

Lysophosphatidic acid (LPA) is a lipid messenger mediated by G protein-coupled receptors (LPAR1-6). It is involved in the pathogenesis of certain chronic inflammatory and autoimmune diseases. In addition, it controls the self-renewal and differentiation of stem cells. Recent research has demonstrated the close relationship between periodontitis and various diseases in the human body. However, the precise role of LPA in the development of periodontitis has not been studied. We identified that LPAR1 was highly expressed in human periodontal ligament stem cells (PDLSCs). In periodontitis-mimicking conditions with Porphyromonas gingivalis -derived lipopolysaccharide (Pg-LPS) treatment, PDLSCs exhibited a considerable reduction in the cellular viability and osteogenic differentiation potential, in addition to an increase in the inflammatory responses including tumor necrosis factor-α and interleukin-1β expression and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation. Of the various LPAR antagonists, pre-treatment with AM095, an LPAR1 inhibitor, showed a positive effect on the restoration of cellular viability and osteogenic differentiation, accompanied by a decrease in NF-κB signaling, and action against Pg-LPS. These findings suggest that the modulation of LPAR1 activity will assist in checking the progression of periodontitis and in its treatment.

Clear cell odontogenic carcinoma (CCOC), a very rare neoplasm located mostly in the mandible, has been regarded as a benign tumor. However, due to the accumulation of case reports, CCOC has been reclassified as a malignant entity by the World Health Organization. Patients with CCOC present with regional swelling and periodontal indications with variable pain, often remaining misdiagnosed for a long period. CCOC has slow growth but aggressive behavior, requiring radical resection. Histologic analysis revealed the monophasic, biphasic, and ameloblastic types of CCOC with clear cells and a mixed combination of polygonal and palisading cells. At the molecular level, CCOC shows the expression of cytokeratin and epithelial membrane antigen, along with markers that assign CCOC to the sarcoma family. At the genetic level, Ewing sarcoma breakpoint region 1-activating transcription factor 1 fusion is regarded as the key feature for identification. Nevertheless, the scarcity of cases and dependence on histological data delay the development of an efficient therapy. Regarding the high recurrence rate and the potential of distant metastasis, further characterization of CCOC is necessary for an early and accurate diagnosis.

We develop forecast models of daily probabilities of major flares (M- and X-class) based on empirical relationships between photospheric magnetic parameters and daily flaring rates from May 2010 to April 2018. In this study, we consider ten magnetic parameters characterizing size, distribution, and non-potentiality of vector magnetic fields from Solar Dynamics Observatory (SDO)/Helioseismic and Magnetic Imager (HMI) and Geostationary Operational Environmental Satellites (GOES) X-ray flare data. The magnetic parameters are classified into three types: the total unsigned parameters, the total signed parameters, and the mean parameters. We divide the data into two sets chronologically: 70% for training and 30% for testing. The empirical relationships between the parameters and flaring rates are used to predict flare occurrence probabilities for a given magnetic parameter value. Major results of this study are as follows. First, major flare occurrence rates are well correlated with ten parameters having correlation coefficients above 0.85. Second, logarithmic values of flaring rates are well approximated by linear equations. Third, using total unsigned and signed parameters achieved better performance for predicting flares than the mean parameters in terms of verification measures of probabilistic and converted binary forecasts. We conclude that the total quantity of non-potentiality of magnetic fields is crucial for flare forecasting among the magnetic parameters considered in this study. When this model is applied for operational use, it can be used using the data of 21:00 TAI with a slight underestimation of 2–6.3%.

The growing demand for nano-structured composite materials and sustainable processes for next generation CO2 capture technologies has necessitated the need to develop novel and cost-effective synthetic routes for solid CO2 adsorbents based on hypercross-linked polymers (HCPs) and reduced graphene oxide (RGO) microporous sorbent materials with improved physico-chemical properties. The most important selection is modification of the synthesized microporous sorbent materials by the incorporation of RGO, giving rise to composite materials that combine the properties of both. These hybrid materials will be of great potential for carbon capture and storage (CCS) applications, especially for post-combustion CO2 capture, owing to the increase in CO2 capturing efficiency and selectivity to CO2 compared to other flue gases. Herein, we report a facile and effective approach for fabrication of HCPs-supported reduced graphene oxide composites. The microporous HCPs was synthesized using 4,4′-bis(chloromethyl)-1,1′-biphenyl monomer by Friedel–Crafts alkylation. The RGO was prepared by modified Hammers method. The as-synthesized composites were characterized by TEM, SEM, FTIR, TGA and N2 adsorption–desorption isotherm. The HCP/RGO composite showed maximum CO2 adsorption of 5.1 wt% than the HCPs alone at 40 °C and 1 atm.

The global mushroom industry has grown rapidly in recent years in terms of beneficial effects, market value, and demand. India has a wide range of agro-climatic conditions and is largely an agricultural country with a cultivated area of about 4.37 %, generating about 620 million tons of agro waste annually. Mushroom cultivation not only helps recycle agro wastes, but also fills the nutritional gap prevalent among a large population of India. Recently, government industrial policy and creative innovation has promoted research and other endeavors aiming towards the cultivation of mushrooms. Mushroom cultivation in India was initiated in Solan, in the mid-sixties. Mushroom cultivation has been successful in temperate regions of the Himalayas, the Western Ghats, and the hills of northeast India. Recently, many unemployed people have begun to adopt mushroom cultivation as a means of self-employment. It is high time that Indian mushroom cultivators and consumers became aware of the nutritional and medicinal values of cultivated and wild species of mushrooms. The total mushroom production in India between 2010 and 2017 was approximately 0.13 million tons, accounting for a 4.3% increase in the average growth rate of mushrooms per annum. In particular, the total production of white button mushrooms is the highest, with a share of about 73% of total mushroom production. In this review article, we have analyzed the current scenario of the Indian mushroom industry and its contribution to the economic growth of the country.

Rats are an important laboratory animal for biomedical research. Though rats have some physiology and genetic similarities to human, several technical issues such as delicate in vitro culture system and low survival rate after pronuclear microinjection have hindered the development of transgenic rat generation. Accordingly, in this study, to produce transgenic rat, we established transposon-mediated insertional mutagenesis by cytoplasmic microinjection. The sleeping beauty transposon (SB) and SB-transposase recognize the precise genome integration into a TA nucleotide by ‘cut-and-paste’ mechanism. It mediates stable integration and reliable long-term expression. DNA, 0.4ng/ul SB vector (IR/DR-EF1a-eGFP-2A-IL2-pA-IR/DR) and mRNA, 5ng/ul SB-transposase were injected to 1-cell stage embryo and one transgenic rat was generated after full-term gestation. To confirm the genome insertion, GFP was detected by PCR. Further, this method was applied to generate transgenic rats producing Cas9 protein. DNA, 0.4ng/ul SB vector (IR/DR-CAG-Cas9-2A-eGFP-pA-IR/DR) and mRNA, 5ng/ul SB-transposase were injected to 1-cell stage embryo. Some of the in vitro cultured embryos showed GFP positive at blastocyst stage and Cas9 sequence was detected by PCR. One stillbirth pup was born to date and genome PCR on Cas9 was positive. In summary, the SB transposon system could be a highly effective method that contribute to the production of transgenic rats. If the protocols will be optimized, we successfully generated efficiently transgenic rats for human models by SB system. This work was supported by BK21 PLUS Program for Creative Veterinary Science, the National Research Foundation of Korea (2017R1A2B3004972) and the Technology Development Program (S2566872) by MSS.

Xyrosaris Meyrick, 1907 is a small genus of the family Yponomeutidae, established by Meyrick (1907) for its type species Xyrosaris dyropa Meyrick, 1907. A total of nine species were recognized in the world. Most of species of the genus have been described by Meyrick at the early 20th century. Up to date, X. lichneuta Meyrick, 1918 has been reported from Korea. In the present study, we review the genus Xyrosaris from Korea, with two new species: X. sp.1 and X. sp.2. Key to species, illustrations of adult, male and female genitalia, diagnosis and short description are provided.