Pellino, a highly conserved E3 ubiquitin ligase, is known to mediate ubiquitination of phosphorylated Interleukin-1 receptor-related kinase (IRAK) homologs in Toll signaling pathway. To understand the immunological function of TmPellino, we screened the knockdown efficiency of TmPellino by injecting TmPellino-specific dsRNA into T. molitor larvae. Subsequently, we investigated the larval mortality and the tissue-specific expression patterns of antimicrobial peptide (AMP) genes against microbial challenges. Interestingly, the results indicate that the expression of many AMP genes was upregulated in the Malpighian tubules of TmPellino-silenced T. molitor larvae. This study may provide basic information to understand how Tmpellino regulates AMPs production in T. molitor.

본 연구는 소양강댐 하류에서 서식하는 생태계교란 생물 종인 브라운송어와 그 먹이원으로 이용되는 저서성 대형무척추동물에 대한 파악을 위해 2022년부터 2023년까지 총 8회에 걸쳐 소양강댐 하류(St.1~St.3)와 지류 (St.4)에 대해 브라운송어와 공서종, 브라운송어의 위 내용물, 저서성 대형무척추동물의 종조성 및 기능군 분석을 실시하였다. 저서성 대형무척추동물의 경우, 하루살이목에서 가장 많은 분류군이 확인되었으며(27.1%), 그 중 붙는 무리(CL)와 헤엄치는 무리(SW)가 높은 비율을 차지하는 것으로 확인되었다. 브라운송어 채집 결과, 전장은 26∼246mm까지 총 105개체가 채집되었으며, 전장-체중 관계의 매개변수 b값이 3을 초과하여 안정적인 성장이 이루어지는 것으로 확인되었다. 브라운송어의 위 내용물에 대한 먹이원 분석 결과, 빙어(0.2%, TL: 246mm)와 육상곤충(2.7%, TL: 154mm, 183∼185mm)을 섭식한 개체는 매우 적었으며 상대적으로 전장이 큰 개체에서 확인 되었다. 대부분 수서곤충(73.8%)과 물 속에서 서식하는 비곤충류(23.3%)를 섭식하는 것으로 나타났다. 브라운 송어의 전장에 따른 먹이 섭식 패턴을 파악하기 위해 위 내용물에서 확인된 종들과의 상관분석을 실시한 결과, 브라운송어의 먹이원 중 유수성 환경 선호 종들의 경우 전장과 양의 상관관계(p<0.05)를 나타낸 반면, 모래 기질 이하의 흐름이 적은 서식처를 선호하는 종들의 경우 전장과 음의 상관관계(p<0.05)를 나타냈다.

Taguchi method is one of the most popular approaches for design optimization such that performance characteristics become robust to uncontrollable noise variables. However, most previous Taguchi method applications have addressed a single-characteristic problem. Problems with multiple characteristics are more common in practice. The multi-criteria decision making(MCDM) problem is to select the optimal one among multiple alternatives by integrating a number of criteria that may conflict with each other. Representative MCDM methods include TOPSIS(Technique for Order of Preference by Similarity to Ideal Solution), GRA(Grey Relational Analysis), PCA(Principal Component Analysis), fuzzy logic system, and so on. Therefore, numerous approaches have been conducted to deal with the multi-characteristic design problem by combining original Taguchi method and MCDM methods. In the MCDM problem, multiple criteria generally have different measurement units, which means that there may be a large difference in the physical value of the criteria and ultimately makes it difficult to integrate the measurements for the criteria. Therefore, the normalization technique is usually utilized to convert different units of criteria into one identical unit. There are four normalization techniques commonly used in MCDM problems, including vector normalization, linear scale transformation( max-min, max, or sum). However, the normalization techniques have several shortcomings and do not adequately incorporate the practical matters. For example, if certain alternative has maximum value of data for certain criterion, this alternative is considered as the solution in original process. However, if the maximum value of data does not satisfy the required degree of fulfillment of designer or customer, the alternative may not be considered as the solution. To solve this problem, this paper employs the desirability function that has been proposed in our previous research. The desirability function uses upper limit and lower limit in normalization process. The threshold points for establishing upper or lower limits let us know what degree of fulfillment of designer or customer is. This paper proposes a new design optimization technique for multi-characteristic design problem by integrating the Taguchi method and our desirability functions. Finally, the proposed technique is able to obtain the optimal solution that is robust to multi-characteristic performances.

Today, as technology advances and market competition for products intensifies, the product design to improve customer satisfaction by accurately identifying customer needs is emerging as a very important issue for company. Accordingly, the customer-oriented or customer-centered design that maximizes customer satisfaction by grasping and analyzing customer requirements is in the spotlight as an important design theory. In this study, the customer-oriented design is defined as finding the optimal value of design variable with the maximum overall customer satisfaction while minimizing the difference in individual customer satisfaction responded to various customers from multiple product quality characteristics from the perspective of robust design. Therefore, this study presents a new method for modeling the customer preference structure as the different sets of desirability functions for multiple quality characteristics and proposes a new customer-oriented design approach by applying the desirability functions to Taguchi’s robust design process to deal with multi-characteristic design problem. Finally, the proposed method is illustrated with the Kansei engineering design problem of wine glass.

현대 사회가 복잡해지고 기술이 진보하면서 하나의 제품을 설계할 때 단일 특성이 아닌 다 특성을 갖는 제품을 설계하는 것이 요구되고 있다. 다 특성 설계 최적화 문제에서는 설계 변수의 최적값이 특성별로 다르게 되는 현상이 발생하고 이에 따라 하나의 특성에 대한 결과값이 좋아지면 다른 특성에 대한 결과값이 나빠지는 경우에 대해서 trade-off가 필요하다. 따라서 다 특성을 갖는 제품 설계 시 각각의 특성에 대한 성능을 최대화할 수 있는 효율적인 설계 방법의 필요성이 중요한 문제로 떠오르고 있다. 다 특성을 갖는 제품을 설계하는 연구는 오래전부터 중요한 문제로 인식되어 왔는데, 단일 특성에 대해 성능을 최대화하며 강건한 설계를 할 수 있는 다구치 기법과 다기준/다특성 의사 결정 방법인 TOPSIS(Technique for Order of Preference by Similarity to Ideal Solution), GRA(Grey relational analysis), PCA(Principle Component Analysis), Fuzzy Logic System을 통합한 연구들이 진행되어 왔다. 하지만 기존 연구들은 각각의 설계 대안들에 대해 다 특성 결과값에 대한 점수를 통합하여 종합 점수를 비교해 최적의 설계안을 도출할 뿐 설계자의 호감도에 대한 정보는 설계에 반영하지 못한다. 따라서 본 연구에서는 다구치 기법을 기반으로 각각의 특성들에 대한 설계자의 서로 다른 호감도를 함수로써 표현하여 강건성과 함께 설계자의 호감도를 동시에 설계에 반영할 수 있는 방법을 제안한다.

Today, as technology advances and market competition for products intensifies, the design to improve product satisfaction by accurately identifying customer requirements is emerging as a very important problem for company. Accordingly, Customer-Oriented Design, that maximizes customer satisfaction by grasping and analyzing customer requirements, is in the spotlight as an important design theory. In this study, Customer- Oriented Design is defined as finding the optimal value of the design variable with the maximum overall customer satisfaction while minimizing the difference in individual customer satisfaction responded to various customers from multiple product quality characteristics from the perspective of robust design. Therefore, in this work, we present a new method for generating a Desirability Function for each quality characteristic to deal with the multi characteristic parameter design with multiple quality characteristics. And we propose a new Customer-Oriented Design methodology that applies these Desirability Functions to Taguchi’s parameter design process.

The fuel fabrication facility has been built and is being operated by KAERI since licensing research reactor fuel fabrication in 2004. After almost 20 years of operation, outdated equipment for fabrication or inspection has been replaced by automated, digitalized ones to assure a higher quality of nuclear fuels. However, the generation of a large amount of radioactive waste is another concern for the replacement in terms of its volume and various types of it that should be categorized before disposal. The regulatory body, NSSC (Nuclear Safety and Security Commission) released a notice related to the classification of radioactive wastes, and most accessory equipment can be classified into the clearance levels, called self-disposal waste. In this study, the practice of self-disposal of metal radioactive waste is carried out to reduce its volume and downgrade its radioactivity. For metal radioactive waste, which is expected to occupy the most amount, analysis status and legal limitations were performed as follows: First, the disposal plan was established after an investigation of the use history for equipment. Second, those were classified by types of materials, and their surface radio-contamination was measured for checking self-disposable or not. After collecting data, the plan for the self-disposal was written and submitted to the Korea Institute of Nuclear Safety (KINS) for approval.

‘Yuyeon’, a new ruminant-palatable barley cultivar, was developed from the cross between ‘Suwon311’ and SB86648〔CMB81A-2149 / Saeolbori // Buheung / Gangbori / 5 / Bera-olbori / Horisee-Buheung // bengei / 3 / Olbori-Daechi-Hagane / 4 / T.beared〕by the Honam Agricultural Research Institute (HARI), NICS, RDA in 2006. Among the cross made in 1996, a promising line, SB961012-B-B-1, showed good characteristics in potential forage yield in the yield trial tested in Suwon in 2001. In 2004, it was designated as ‘Suwon 406’ and placed in regional yield trials at five locations around Korea for three years from 2004 to 2006, and was released as the name of ‘Yuyeon’. It belong to maturity group Ⅲ and shows erect leaf type and compact spike with types of hoods. Its heading date was Apr. 27, which was 2 days earlier than that of the check cultivar ‘Olbori’ (‘Sunwoo’), and early dough stage, which was May. 30 which was same date of that of the check cultivar. ‘Yuyeon’ showed weaker winter hardiness, but better resistance to lodging, shattering and BaYMV than those of check cultivar. It showed higher crude protein content, grade of silage quality than those of check cultivar. The average forage yield in the regional yield trial was 10.47 MT/ha, which was 1% lower than that of the check cultivar. Cattles fed with silage from ‘Yuyeon’ (hood type) showed higher feed concentrate requirement (12.9 kg/day/body) and daily weight gain (1.46 kg/day) than those of cattles fed with silage from ‘Olbori’ (rough awn). This cultivar would be suitable for the area whose daily minimum temperature was above -8℃ in January in Korean peninsula.

A ruminant-palatable barley cultivar, Soman (Hordeum vulgare L.), with early maturing, high silage quality and high yield, was developed from the cross between SB79124〔Bengei-Ginomeo-Buheung-Morsen-Hagane/Gangbori〕and SB77189 〔Samjug/Riso82//Olbori〕in 1994. A promising line “SB941067-B-B-B-45” was selected in 1998. It performed well in the preliminary and advanced yield trials for two years from 2002 to 2003. The line was designated ‘Suwon 404’ through the regional yield trials (RYT) at six sites for three years from 2004 to 2006. Its growth habit was winter type (Ⅲ) with greenish leaves, medium culm and compact density of spike setting. Its heading date and early dough stage were Apr. 23 and May 26 respectively, which were 6 days earlier than those of check cultivar ‘Olbori’. ‘Soman’ showed weaker winter hardiness, but better resistance to lodging, shattering, and BaYMV (Barley yellow mosaic virus) than those of check cultivar ‘Sunwoo’. It showed 1.7% higher TDN content on whole-crop-silage than that of check cultivar ‘Sunwoo’. Its average of forage yield in regional yield trials was 10.31 MT/ha, which was 4% higher than that of the check cultivar.