본 연구에서는 예비 마스터가드너를 선발, 정보를 전달하고 활동을 촉진시켜 프로그램 진행을 위한 실질적인 관리를 담당 하는 코디네이터 양성을 위해 데이컴 분석을 통하여 마스터가드너 코디네이터의 전문성 기준과 직무모형을 제시하였다. 1, 2차 데이컴 위원회를 통해 임무, 작업, 작업 요소를 도출하고 작업에 필요한 지식, 기술, 태도를 확정하였다. 도출된 임무 및 작업 등을 바탕으로 마스터가드너 교육을 담당하고 있는 시군 농업기술센터 농촌지도사를 대상으로 직무 수행평가를 시행하였다. 마스터가드너 코디네이터 직무분석 결과, <A. 정원문화 이해>, <B. 정원기술 이해>, <C. 봉사활동 이해>, <D. 교육과정 개발>, <E. 교육과정 운영>, <F. 교육사후 관리>, <G. 전문성 개발> 등 7개의 임무를 도출하였다. 또한 <A. 정원 문화 이해>의 4개, <B. 정원 기술 이해>의 4개, <C. 봉사 활동 이해>의 6개, <D. 교육과정 개발>의 6개, <E. 교육과정 운영>의 8개, <F. 교육 사후 관리>의 6개, <G. 전문성 개발> 의 7개 작업까지 총 41개의 작업과 173개의 작업 요소를 도출 하였다. 이를 토대로 작업과 지식, 기술, 태도의 관계를 분석 하고 매트릭스로 제작하여 마스터가드너 코디네이터 직무 활동에 사용하도록 하였다. 본 연구의 의의는 마스터가드너 코디네이터의 역할과 활동 범위를 설정을 위한 직무모형을 제안함으로써 향후 마스터가드너 코디네이터 직무교육 표준화 기준을 수립하고 일자리 영역을 구축하는 등 후속 연구를 촉진 할 수 있는 토대될 수 있을 것이라 사료된다.

본 연구의 목적은 온도와 토양수분에 따른 마늘의 생육, 생리장해 및 수량에 미치는 영향을 구명하고자 실시 하였다. 실험은 온도가 6℃ 차이가 나는 온실에서 수행 되었는데, 정식부터 수확까지 외기(A)보다 3℃(A+3℃), 6℃(A+6℃) 고온조건, 토양수분은 적습(OI) 대비 수확기 무렵의 다습(EI) 처리를 하였다. 그 결과, 마늘 생육특성은 온도와 토양수분 조건(0.34m3/m3)에 따라 고도의 유의성을 보여 고온일수록 그리고 적습 처리구일수록 컸다. 초장은 외기보다 A+6℃-OI 처리구에서 47.4cm로 가장 컸으며, 엽장과 엽폭 역시 외기보다 A+6℃-OI 처리구가 각각 16.1cm, 2.4cm로 가장 컸다. 마늘재배 기간 중 외기보다 3℃, 6℃ 고온조건이 되면 스펀지마늘 발생율이 높아져 A+6℃-OI 처리구는 12.9% 발생하였고, A-EI 처리구에서는 전혀 발생하지 않았으며, 인편무게와 1쪽당 무게는 A+6℃ 고온구에서 크게 감소하여 수량이 외기 대비 A+6℃ 처리구는 평균 51%, A+3℃ 처리구는 평균 22% 감소하였다. 따라서, 마늘재배시 외기보다 6℃ 고온조건과 다습조건이 되면 상품수량 감소하고, 생리장해 발생이 많아지는 것으로 나타났다.

The objective of this study was to determine the effect of macrophages on growth of human colon cancer cells. The results showed that co-culture of colon cancer cells with macrophages inhibited the growth of colon cancer cells (HCT116 and SW620) depending on the number of macrophages, RAW 264.7 cells, and activated THP-1 cells accompanied by down regulation of pSTAT3 in cancer cells. We also found that expression and release of cancer cell growth inhibitory cytokines, IL-1 receptor antagonist (IL-1ra) and IL-10, was increased in macrophages. Blocking of the STAT3 pathway with specific inhibitor and siRNA of STAT3 abolished the growth of colon cancer cells and expression of IL-1ra and IL-10. In addition, neutralization of IL-1ra and IL-10 with antibodies resulted in reversal of macrophage-induced inhibition of cancer cell growth. These data showed that IL-1ra and IL-10 released from macrophages inhibit growth of colon cancer cells through inhibition of the STAT3 pathway.

Background : Tooth vitality is reflected by the health of dental pulp. Schisandrin C is a natural compound extracted from the fruit of Schisandra chinensis which has anti-inflammatory and anti-oxidant properties. The role of Schisandrin C on human dental pulp cells (HDPCs) has not been studied yet. This study examined the properties of Schisandrin C as an anti-inflammatory and anti-oxidant compound, and whether its characteristics promote mitochondrial biogenesis in HDPCs. Methods and Results : HDPCs were extracted from fresh third molars and cultured. Reactive oxidative stress (ROS) and nitric oxide (NO) formation were analyzed by a Muse cell analyzer. Western blotting and gelatin zymography were used to identify the presence of anti-oxidants, as well as inflammatory and mitochondrial biogenesis. Confocal microscopy was used for the detection of mitochondrial activity. Schisandrin C inhibited lipopolysaccharide (LPS)-stimulated inflammatory molecules; intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), matrix metalloproteinase-2 and -9 (MMP-2/9), NO production, ROS formation and the mitogen-activated protein (MAPK) pathway through minimizing the nuclear factor kappa B (NF-kB) translocation. Schisandrin C increased the expression of superoxide dismutase (SOD) enzymes as well as heme oxygenase-1 (HO-1) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1a) through the phosphorylated-protein kinase B (p-AKT) and nuclear factor erythroid 2-related factor-2 (Nrf-2) pathways. The anti-inflammatory and anti-oxidant properties of Schisandrin C promoted mitochondrial biogenesis. Conclusions : These results suggest that Schisandrin C may be used as an anti-inflammatory compound to reduce oral inflammation such as pulpitis.

In animal development, the mechanisms by which localized factors and organelles in egg cytoplasm were exactly distributed into each daughter cell are essential for formation of various cell types. During ascidian Halocynthia roretzi embryogenesis, ooplasmic mitochondria were mainly segregated into muscle and neural precursor cells. At the 32-cell stage, localized mitochondria in the B6.2 blastomeres were preferentially distributed into the B7.4 muscle precursors compared with the B7.3 mesenchyme/ notochord precursors. When the B6.2 blastomeres were isolated from the early 32-cell stage embryos and then allowed to divide 2 times of cell division, the resultant partial embryos showed symmetric distribution of mitochondria, and the partial embryos were composed of equal size cells. In normal development, cell fates of the B7.3 blastomere were correlated with the unequal cleavage of B6.2 lineage cells that normally occurs in the next two-cell division stages to produce a large B8.5 mesenchyme and a small B8.6 notochord cell. Mitochondria are distributed asymmetrically in both cells. When embryos were treated with FGF receptor inhibitor SU5402 and MEK inhibitor U0126 between the 32-cell and the early 64-cell stages, the resultant embryos showed equal cleavage pattern and symmetric distribution of mitochondria in daughter cells of the B6.2 blastomeres. However, blocking of Nodal and Notch signaling did not affect the cell division pattern and mitochondrial distribution in the B6.2 lineage blastomeres between the 32-cell and 110-cell stages. Therefore, it is likely that FGF/MEK signaling is involved in asymmetric distribution of mitochondria and unequal cleavage of the B6.2 lineage blastomeres in ascidian embryo.

Soybean self-sufficiency in Korea was 22.5% in year 2011, and as free trade agreement between Korea and US comes into effect, the amount of soybean importation increases. In 2012, paddy field soybean cultivation was over 0.1 million ha and it is expected to increase continuously due to rise in market price of soybean. Moreover, double cropping system including paddy field soybean is widely adopted nationwide, but studies on appropriate cultivar for this environment are insufficient. In this research, the effect of planting date and different cultivars on soybean growth and yield was investigated for three planting dates (June 20, July 5, and July 20) with 15 cultivars. According to ANOVA test, soybean yield was significantly different depending on sowing date and cultivars and interaction between sowing date and cultivars was also detected, meaning each of cultivars resulted different yield depending on sowing date. When planted on July 20, stem length, nod number, branch number and grain weight was decreased because of short growing period, resulting in yield decrease. Percentage of yield obtained from July 20 in contrast to that of June 20 showed that Pungwon, Nampung and Daepung recorded the least yield decrease and the highest yield when planted late. Evaluation of late-sowing-adaptable soybean cultivar in paddy field is now being carried on following last year.